LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 36

Search options

  1. Book ; Thesis: Hepatic stellate cells induce gut tropism of CD8+ T lymphocytes

    Maschmeyer, Patrick

    2015  

    Author's details vorgelegt von Diplom-Ingenieur, Master of Science Patrick Maschmeyer
    Language German
    Size XXII, 176 Seiten, Illustrationen, 21 cm
    Publishing place Berlin
    Publishing country Germany
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Dissertation, Technische Universität Berlin, 2015
    HBZ-ID HT019470996
    Database Catalogue ZB MED Medicine, Health

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2017 D 1089 order for loan Magazin

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: ATACing single cells with phages.

    Maschmeyer, Patrick / Haas, Simon

    Molecular cell

    2022  Volume 82, Issue 2, Page(s) 234–236

    Abstract: Fiskin et al. (2021) developed a "multi-omics" approach that integrates phage-displayed single-domain antibodies ("nanobodies") with the assay for transposase-accessible chromatin (PHAGE-ATAC) to simultaneously determine protein expression, chromatin ... ...

    Abstract Fiskin et al. (2021) developed a "multi-omics" approach that integrates phage-displayed single-domain antibodies ("nanobodies") with the assay for transposase-accessible chromatin (PHAGE-ATAC) to simultaneously determine protein expression, chromatin accessibility, and mitochondrial DNA mutations (for clonal tracing) in single cells.
    MeSH term(s) Bacteriophages/genetics ; Chromatin ; High-Throughput Nucleotide Sequencing ; Sequence Analysis, DNA ; Transposases
    Chemical Substances Chromatin ; Transposases (EC 2.7.7.-)
    Language English
    Publishing date 2022-01-22
    Publishing country United States
    Document type Journal Article ; Comment
    ZDB-ID 1415236-8
    ISSN 1097-4164 ; 1097-2765
    ISSN (online) 1097-4164
    ISSN 1097-2765
    DOI 10.1016/j.molcel.2021.12.028
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 5055: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article: ATACing single cells with phages

    Maschmeyer, Patrick / Haas, Simon

    Molecular cell. 2022 Jan. 20, v. 82, no. 2

    2022  

    Abstract: Fiskin et al. (2021) developed a “multi-omics” approach that integrates phage-displayed single-domain antibodies (“nanobodies”) with the assay for transposase-accessible chromatin (PHAGE-ATAC) to simultaneously determine protein expression, chromatin ... ...

    Abstract Fiskin et al. (2021) developed a “multi-omics” approach that integrates phage-displayed single-domain antibodies (“nanobodies”) with the assay for transposase-accessible chromatin (PHAGE-ATAC) to simultaneously determine protein expression, chromatin accessibility, and mitochondrial DNA mutations (for clonal tracing) in single cells.
    Keywords chromatin ; mitochondrial DNA ; protein synthesis
    Language English
    Dates of publication 2022-0120
    Size p. 234-236.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 1415236-8
    ISSN 1097-4164 ; 1097-2765
    ISSN (online) 1097-4164
    ISSN 1097-2765
    DOI 10.1016/j.molcel.2021.12.028
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 2005/501: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  4. Article ; Online: Murine T-Cell Transfer Colitis as a Model for Inflammatory Bowel Disease.

    Maschmeyer, Patrick / Zimmermann, Jakob / Kühl, Anja Andrea

    Methods in molecular biology (Clifton, N.J.)

    2021  Volume 2285, Page(s) 349–373

    Abstract: Inflammatory bowel disease (IBD) is a group of severe chronic inflammatory conditions of the human gastrointestinal tract. Murine models of colitis have been invaluable tools to improve the understanding of IBD development and pathogenesis. While the ... ...

    Abstract Inflammatory bowel disease (IBD) is a group of severe chronic inflammatory conditions of the human gastrointestinal tract. Murine models of colitis have been invaluable tools to improve the understanding of IBD development and pathogenesis. While the disease etiology of IBD is complex and multifactorial, CD4
    MeSH term(s) Adoptive Transfer ; Animals ; Cell Separation ; Colitis/immunology ; Colitis/metabolism ; Colitis/pathology ; Colon/immunology ; Colon/metabolism ; Colon/pathology ; Disease Models, Animal ; Flow Cytometry ; Homeodomain Proteins/genetics ; Mice, Inbred C57BL ; Mice, Knockout ; Research Design ; T-Lymphocytes, Helper-Inducer/immunology ; T-Lymphocytes, Helper-Inducer/metabolism ; T-Lymphocytes, Helper-Inducer/transplantation ; Workflow ; Mice
    Chemical Substances Homeodomain Proteins ; RAG-1 protein (128559-51-3)
    Language English
    Publishing date 2021-04-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-1311-5_26
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article: Nachrichten der Gesellschaft für Kinder- und Jugendrheumatologie

    Niewerth, Martina / Maschmeyer, Patrick / Reiser, Christiane

    Arthritis und Rheuma

    2020  Volume 40, Issue 06, Page(s) 453–455

    Language German
    Publishing date 2020-12-01
    Publisher Georg Thieme Verlag KG
    Publishing place Stuttgart ; New York
    Document type Article
    ZDB-ID 2223481-0
    ISSN 2567-5753 ; 0176-5167
    ISSN (online) 2567-5753
    ISSN 0176-5167
    DOI 10.1055/a-1287-2885
    Database Thieme publisher's database

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: Long-Chain Hydrocarbons from Nonthermal Plasma-Driven Biogas Upcycling.

    Knezevic, Josip / Zhang, Tianqi / Zhou, Renwu / Hong, Jungmi / Zhou, Rusen / Barnett, Christopher / Song, Qiang / Gao, Yuting / Xu, Wanping / Liu, Dingxin / Proschogo, Nicholas / Mohanty, Biswaranjan / Strachan, Jyah / Soltani, Behdad / Li, Fengwang / Maschmeyer, Thomas / Lovell, Emma C / Cullen, Patrick J

    Journal of the American Chemical Society

    2024  

    Abstract: The burgeoning necessity to discover new methodologies for the synthesis of long-chain hydrocarbons and oxygenates, independent of traditional reliance on high-temperature, high-pressure, and fossil fuel-based carbon, is increasingly urgent. In this ... ...

    Abstract The burgeoning necessity to discover new methodologies for the synthesis of long-chain hydrocarbons and oxygenates, independent of traditional reliance on high-temperature, high-pressure, and fossil fuel-based carbon, is increasingly urgent. In this context, we introduce a nonthermal plasma-based strategy for the initiation and propagation of long-chain carbon growth from biogas constituents (CO
    Language English
    Publishing date 2024-04-30
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3155-0
    ISSN 1520-5126 ; 0002-7863
    ISSN (online) 1520-5126
    ISSN 0002-7863
    DOI 10.1021/jacs.4c01641
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 4' 55/32: Show issues
    Z 7.3: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  7. Article ; Online: GARP on hepatic stellate cells is essential for the development of liver fibrosis.

    Zhang, Xiaolong / Sharma, Pankaj / Maschmeyer, Patrick / Hu, Yu / Lou, Mumeng / Kim, Jessica / Fujii, Hodaka / Unutmaz, Derya / Schwabe, Robert F / Winau, Florian

    Journal of hepatology

    2023  Volume 79, Issue 5, Page(s) 1214–1225

    Abstract: Background & aims: Glycoprotein A repetitions predominant (GARP) is a membrane protein that functions as a latent TGF-β docking molecule. While the immune regulatory properties of GARP on blood cells have been studied, the function of GARP on tissue ... ...

    Abstract Background & aims: Glycoprotein A repetitions predominant (GARP) is a membrane protein that functions as a latent TGF-β docking molecule. While the immune regulatory properties of GARP on blood cells have been studied, the function of GARP on tissue stromal cells remains unclear. Here, we investigate the role of GARP expressed on hepatic stellate cells (HSCs) in the development of liver fibrosis.
    Methods: The function of GARP on HSCs was explored in toxin-induced and metabolic liver fibrosis models, using conditional GARP-deficient mice or a newly generated inducible system for HSC-specific gene ablation. Primary mouse and human HSCs were isolated to evaluate the contribution of GARP to the activation of latent TGF-β. Moreover, cell contraction of HSCs in the context of TGF-β activation was tested in a GARP-dependent fashion.
    Results: Mice lacking GARP in HSCs were protected from developing liver fibrosis. Therapeutically deleting GARP on HSCs alleviated the fibrotic process in established disease. Furthermore, natural killer T cells exacerbated hepatic fibrosis by inducing GARP expression on HSCs through IL-4 production. Mechanistically, GARP facilitated fibrogenesis by activating TGF-β and enhancing endothelin-1-mediated HSC contraction. Functional GARP was expressed on human HSCs and significantly upregulated in the livers of patients with fibrosis. Lastly, deletion of GARP on HSCs did not augment inflammation or liver damage.
    Conclusions: GARP expressed on HSCs drives the development of liver fibrosis via cell contraction-mediated activation of latent TGF-β. Considering that systemic blockade of TGF-β has major side effects, we highlight a therapeutic niche provided by GARP and surface-mediated TGF-β activation. Thus, our findings suggest an important role of GARP on HSCs as a promising target for the treatment of liver fibrosis.
    Impact and implications: Liver fibrosis represents a substantial and increasing public health burden globally, for which specific treatments are not available. Glycoprotein A repetitions predominant (GARP) is a membrane protein that functions as a latent TGF-β docking molecule. Here, we show that GARP expressed on hepatic stellate cells drives the development of liver fibrosis. Our findings suggest GARP as a novel target for the treatment of fibrotic disease.
    Language English
    Publishing date 2023-06-20
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 605953-3
    ISSN 1600-0641 ; 0168-8278
    ISSN (online) 1600-0641
    ISSN 0168-8278
    DOI 10.1016/j.jhep.2023.05.043
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2027: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article ; Online: Immunological memory in rheumatic inflammation - a roadblock to tolerance induction.

    Maschmeyer, Patrick / Chang, Hyun-Dong / Cheng, Qingyu / Mashreghi, Mir-Farzin / Hiepe, Falk / Alexander, Tobias / Radbruch, Andreas

    Nature reviews. Rheumatology

    2021  Volume 17, Issue 5, Page(s) 291–305

    Abstract: Why do we still have no cure for chronic inflammatory diseases? One reason could be that current therapies are based on the assumption that chronic inflammation is driven by persistent 'acute' immune reactions. Here we discuss a paradigm shift by ... ...

    Abstract Why do we still have no cure for chronic inflammatory diseases? One reason could be that current therapies are based on the assumption that chronic inflammation is driven by persistent 'acute' immune reactions. Here we discuss a paradigm shift by suggesting that beyond these reactions, chronic inflammation is driven by imprinted, pathogenic 'memory' cells of the immune system. This rationale is based on the observation that in patients with chronic inflammatory rheumatic diseases refractory to conventional immunosuppressive therapies, therapy-free remission can be achieved by resetting the immune system; that is, by ablating immune cells and regenerating the immune system from stem cells. The success of this approach identifies antigen-experienced and imprinted immune cells as essential and sufficient drivers of inflammation. The 'dark side' of immunological memory primarily involves memory plasma cells secreting pathogenic antibodies and memory T lymphocytes secreting pathogenic cytokines and chemokines, but can also involve cells of innate immunity. New therapeutic strategies should address the persistence of these memory cells. Selective targeting of pathogenic immune memory cells could be based on their specificity, which is challenging, or on their lifestyle, which differs from that of protective immune memory cells, in particular for pathogenic T lymphocytes. The adaptations of such pathogenic memory cells to chronic inflammation offers entirely new therapeutic options for their selective ablation and the regeneration of immunological tolerance.
    MeSH term(s) Animals ; Humans ; Immune Tolerance ; Immunity, Innate ; Immunologic Memory/immunology ; Immunosuppression/methods ; Immunosuppressive Agents/therapeutic use ; Rheumatic Diseases/drug therapy ; Rheumatic Diseases/immunology
    Chemical Substances Immunosuppressive Agents
    Language English
    Publishing date 2021-04-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2491532-4
    ISSN 1759-4804 ; 1759-4790
    ISSN (online) 1759-4804
    ISSN 1759-4790
    DOI 10.1038/s41584-021-00601-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 6338: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  9. Article ; Online: Clonally expanded PD-1-expressing T cells are enriched in synovial fluid of juvenile idiopathic arthritis patients.

    Vanni, Anna / Mazzoni, Alessio / Semeraro, Roberto / Capone, Manuela / Maschmeyer, Patrick / Lamacchia, Giulia / Salvati, Lorenzo / Carnasciali, Alberto / Farahvachi, Parham / Giani, Teresa / Simonini, Gabriele / Filocamo, Giovanni / Romano, Micol / Liotta, Francesco / Mashreghi, Mir-Farzin / Cosmi, Lorenzo / Cimaz, Rolando / Magi, Alberto / Maggi, Laura /
    Annunziato, Francesco

    European journal of immunology

    2023  Volume 53, Issue 7, Page(s) e2250162

    Abstract: Juvenile idiopathic arthritis (JIA) is the most common chronic rheumatic condition in childhood. The disease etiology remains largely unknown; however, a key role in JIA pathogenesis is surely mediated by T cells. T-lymphocytes activity is controlled via ...

    Abstract Juvenile idiopathic arthritis (JIA) is the most common chronic rheumatic condition in childhood. The disease etiology remains largely unknown; however, a key role in JIA pathogenesis is surely mediated by T cells. T-lymphocytes activity is controlled via signals, known as immune checkpoints. Delivering an inhibitory signal or blocking a stimulatory signal to achieve immune suppression is critical in autoimmune diseases. However, the role of immune checkpoints in chronic inflammation and autoimmunity must still be deciphered. In this study, we investigated at the single-cell level the feature of T cells in JIA chronic inflammation, both at the transcriptome level via single-cell RNA sequencing and at the protein level by flow cytometry. We found that despite the heterogeneity in the composition of synovial CD4
    MeSH term(s) Humans ; Arthritis, Juvenile ; Synovial Fluid ; Programmed Cell Death 1 Receptor ; CD8-Positive T-Lymphocytes ; CD4-Positive T-Lymphocytes ; Inflammation
    Chemical Substances Programmed Cell Death 1 Receptor
    Language English
    Publishing date 2023-05-08
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Comment
    ZDB-ID 120108-6
    ISSN 1521-4141 ; 0014-2980
    ISSN (online) 1521-4141
    ISSN 0014-2980
    DOI 10.1002/eji.202250162
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 489: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 85: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  10. Article: Human regulatory T cells locally differentiate and are functionally heterogeneous within the inflamed arthritic joint.

    Lutter, Lisanne / van der Wal, M Marlot / Brand, Eelco C / Maschmeyer, Patrick / Vastert, Sebastiaan / Mashreghi, Mir-Farzin / van Loosdregt, Jorg / van Wijk, Femke

    Clinical & translational immunology

    2022  Volume 11, Issue 10, Page(s) e1420

    Abstract: Objective: Tregs are crucial for immune regulation, and environment-driven adaptation of effector (e)Tregs is essential for local functioning. However, the extent of human Treg heterogeneity in inflammatory settings is unclear.: Methods: We combined ... ...

    Abstract Objective: Tregs are crucial for immune regulation, and environment-driven adaptation of effector (e)Tregs is essential for local functioning. However, the extent of human Treg heterogeneity in inflammatory settings is unclear.
    Methods: We combined single-cell RNA- and TCR-sequencing on Tregs derived from three to six patients with juvenile idiopathic arthritis (JIA) to investigate the functional heterogeneity of human synovial fluid (SF)-derived Tregs from inflamed joints. Confirmation and suppressive function of the identified Treg clusters was assessed by flow cytometry.
    Results: Four Treg clusters were identified; incoming, activated eTregs with either a dominant suppressive or cytotoxic profile, and GPR56
    Conclusion: Our study reveals a heterogeneous population of Tregs at the site of inflammation in JIA. SF Treg differentiate to a classical eTreg profile with a more dominant suppressive or cytotoxic profile that share a similar TCR repertoire, or towards GPR56
    Language English
    Publishing date 2022-09-30
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 2694482-0
    ISSN 2050-0068
    ISSN 2050-0068
    DOI 10.1002/cti2.1420
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top