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  1. Article ; Online: Lake Erie ice is a repository of organisms.

    Iwaloye, Opeoluwa F / Michaud, Brenna / Alloy, Tessa / D'Souza, Nigel / McKay, R Michael L / Morris, Paul / Gura, Colby / Rogers, Scott O

    Microbiology resource announcements

    2024  Volume 13, Issue 4, Page(s) e0109423

    Abstract: Organism abundance and diversity were assessed in Lake Erie ice samples using sequences derived from a combined metagenomic and metatranscriptomic analysis. The 68,417 unique sequences were from Bacteria (77.5%), Eukarya (22.3%), and Archaea (0.2%) and ... ...

    Abstract Organism abundance and diversity were assessed in Lake Erie ice samples using sequences derived from a combined metagenomic and metatranscriptomic analysis. The 68,417 unique sequences were from Bacteria (77.5%), Eukarya (22.3%), and Archaea (0.2%) and indicated diverse species of organisms from 32 bacterial, 8 eukaryotic, and 2 archaeal taxonomic groups.
    Language English
    Publishing date 2024-02-27
    Publishing country United States
    Document type Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/mra.01094-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Next-generation COVID-19 vaccines: here come the proteins.

    Blakney, Anna K / McKay, Paul F

    Lancet (London, England)

    2021  Volume 397, Issue 10275, Page(s) 643–645

    MeSH term(s) Adult ; COVID-19 ; COVID-19 Vaccines ; Double-Blind Method ; Humans ; Protein Subunits ; SARS-CoV-2 ; Viral Vaccines
    Chemical Substances COVID-19 Vaccines ; Protein Subunits ; Viral Vaccines
    Language English
    Publishing date 2021-01-29
    Publishing country England
    Document type Journal Article ; Comment
    ZDB-ID 3306-6
    ISSN 1474-547X ; 0023-7507 ; 0140-6736
    ISSN (online) 1474-547X
    ISSN 0023-7507 ; 0140-6736
    DOI 10.1016/S0140-6736(21)00258-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: An Overview of Rift Valley Fever Vaccine Development Strategies.

    Kitandwe, Paul Kato / McKay, Paul F / Kaleebu, Pontiano / Shattock, Robin J

    Vaccines

    2022  Volume 10, Issue 11

    Abstract: Rift Valley fever (RVF) is a mosquito-borne viral zoonosis that causes high fetal and neonatal mortality in ruminants and a mild to fatal hemorrhagic fever in humans. There are no licensed RVF vaccines for human use while for livestock, commercially ... ...

    Abstract Rift Valley fever (RVF) is a mosquito-borne viral zoonosis that causes high fetal and neonatal mortality in ruminants and a mild to fatal hemorrhagic fever in humans. There are no licensed RVF vaccines for human use while for livestock, commercially available vaccines are all either live attenuated or inactivated and have undesirable characteristics. The live attenuated RVF vaccines are associated with teratogenicity and residual virulence in ruminants while the inactivated ones require multiple immunisations to induce and maintain protective immunity. Additionally, nearly all licensed RVF vaccines lack the differentiating infected from vaccinated animals (DIVA) property making them inappropriate for use in RVF nonendemic countries. To address these limitations, novel DIVA-compatible RVF vaccines with better safety and efficacy than the licensed ones are being developed, aided fundamentally by a better understanding of the molecular biology of the RVF virus and advancements in recombinant DNA technology. For some of these candidate RVF vaccines, sterilizing immunity has been demonstrated in the discovery/feasibility phase with minimal adverse effects. This review highlights the progress made to date in RVF vaccine research and development and discusses the outstanding research gaps.
    Language English
    Publishing date 2022-10-25
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines10111794
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  4. Article ; Online: Chlamydia trachomatis: Cell biology, immunology and vaccination.

    Murray, Sam M / McKay, Paul F

    Vaccine

    2021  Volume 39, Issue 22, Page(s) 2965–2975

    Abstract: Chlamydia trachomatis is the causative agent of a highly prevalent sexually transmitted bacterial disease and is associated with a number of severe disease complications. Current therapy options are successful at treating disease, but patients are left ... ...

    Abstract Chlamydia trachomatis is the causative agent of a highly prevalent sexually transmitted bacterial disease and is associated with a number of severe disease complications. Current therapy options are successful at treating disease, but patients are left without protective immunity and do not benefit the majority asymptomatic patients who do not seek treatment. As such, there is a clear need for a broad acting, protective vaccine that can prevent transmission and protect against symptomatic disease presentation. There are three key elements that underlie successful vaccine development: 1) Chlamydia biology and immune-evasion adaptations, 2) the correlates of protection that prevent disease in natural and experimental infection, 3) reflection upon the evidence provided by previous vaccine attempts. In this review, we give an overview of the unique intra-cellular biology of C. trachomatis and give insight into the dynamic combination of adaptations that allow Chlamydia to subvert host immunity and survive within the cell. We explore the current understanding of chlamydial immunity in animal models and in humans and characterise the key immune correlates of protection against infection. We discuss in detail the specific immune interactions involved in protection, with relevance placed on the CD4+ T lymphocyte and B lymphocyte responses that are key to pathogen clearance. Finally, we provide a timeline of C. trachomatis vaccine research to date and evaluate the successes and failures in development so far. With insight from these three key elements of research, we suggest potential solutions for chlamydial vaccine development and promising avenues for further exploration.
    MeSH term(s) Animals ; Bacterial Vaccines ; CD4-Positive T-Lymphocytes ; Chlamydia Infections/prevention & control ; Chlamydia trachomatis ; Humans ; Vaccination
    Chemical Substances Bacterial Vaccines
    Language English
    Publishing date 2021-03-24
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 605674-x
    ISSN 1873-2518 ; 0264-410X
    ISSN (online) 1873-2518
    ISSN 0264-410X
    DOI 10.1016/j.vaccine.2021.03.043
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  5. Article: A self-amplifying RNA vaccine provides protection in a murine model of bubonic plague.

    Shattock, Robin John / Andrianaivoarimanana, Voahangy / McKay, Paul F / Randriantseheno, Lovasoa Nomena / Murugaiah, Valarmathy / Samnuan, K / Rogers, Paul / Tregoning, John S / Rajerison, Minoarisoa / Moore, Kristoffer M / Laws, Thomas Robert / Williamson, E Diane

    Frontiers in microbiology

    2023  Volume 14, Page(s) 1247041

    Abstract: Mice were immunized with a combination of self-amplifying (sa) RNA constructs for the F1 and V antigens ... ...

    Abstract Mice were immunized with a combination of self-amplifying (sa) RNA constructs for the F1 and V antigens of
    Language English
    Publishing date 2023-11-02
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2023.1247041
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: An Overview of Rift Valley Fever Vaccine Development Strategies

    Paul Kato Kitandwe / Paul F. McKay / Pontiano Kaleebu / Robin J. Shattock

    Vaccines, Vol 10, Iss 1794, p

    2022  Volume 1794

    Abstract: Rift Valley fever (RVF) is a mosquito-borne viral zoonosis that causes high fetal and neonatal mortality in ruminants and a mild to fatal hemorrhagic fever in humans. There are no licensed RVF vaccines for human use while for livestock, commercially ... ...

    Abstract Rift Valley fever (RVF) is a mosquito-borne viral zoonosis that causes high fetal and neonatal mortality in ruminants and a mild to fatal hemorrhagic fever in humans. There are no licensed RVF vaccines for human use while for livestock, commercially available vaccines are all either live attenuated or inactivated and have undesirable characteristics. The live attenuated RVF vaccines are associated with teratogenicity and residual virulence in ruminants while the inactivated ones require multiple immunisations to induce and maintain protective immunity. Additionally, nearly all licensed RVF vaccines lack the differentiating infected from vaccinated animals (DIVA) property making them inappropriate for use in RVF nonendemic countries. To address these limitations, novel DIVA-compatible RVF vaccines with better safety and efficacy than the licensed ones are being developed, aided fundamentally by a better understanding of the molecular biology of the RVF virus and advancements in recombinant DNA technology. For some of these candidate RVF vaccines, sterilizing immunity has been demonstrated in the discovery/feasibility phase with minimal adverse effects. This review highlights the progress made to date in RVF vaccine research and development and discusses the outstanding research gaps.
    Keywords Rift Valley fever virus ; RVFV ; Rift Valley fever vaccine ; RVF ; Medicine ; R
    Language English
    Publishing date 2022-10-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Charge neutralized poly(β-amino ester) polyplex nanoparticles for delivery of self-amplifying RNA.

    Dastgerdi, Nazgol Karimi / Gumus, Nurcan / Bayraktutan, Hulya / Jackson, Darryl / Polra, Krunal / McKay, Paul F / Atyabi, Fatemeh / Dinarvand, Rassoul / Shattock, Robin J / Martinez-Pomares, Luisa / Gurnani, Pratik / Alexander, Cameron

    Nanoscale advances

    2024  Volume 6, Issue 5, Page(s) 1409–1422

    Abstract: Therapeutic self-amplifying RNA (saRNA) is a promising approach for disease treatment, as it can be administered in lower doses than messenger RNA (mRNA) to achieve comparable protein production levels. However, saRNA requires an appropriate delivery ... ...

    Abstract Therapeutic self-amplifying RNA (saRNA) is a promising approach for disease treatment, as it can be administered in lower doses than messenger RNA (mRNA) to achieve comparable protein production levels. However, saRNA requires an appropriate delivery vehicle to protect it during transit and facilitate its transfection. A widely-adopted approach has been to use polycations to condense these large anionic macromolecules into polyplex nanoparticles, however their high charge density often elicits cytotoxic effects. In this study we postulated that we could improve the potency and tolerability of such delivery vehicles by co-formulating poly(β-amino ester)s saRNA polyplexes with a non-toxic anionic polymer, γ-polyglutamic acid (γ-PGA) to neutralize partially this positive charge. Accordingly, we prepared a poly(β-amino ester) from 1,6-hexanedioldiacrylate (HDDA) and 4-aminobutanol (ABOL) and initially evaluated the physicochemical properties of the binary polyplexes (
    Language English
    Publishing date 2024-01-24
    Publishing country England
    Document type Journal Article
    ISSN 2516-0230
    ISSN (online) 2516-0230
    DOI 10.1039/d3na00794d
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Increasing human monoclonal antibody cloning efficiency with a whole-cell modified immunoglobulin-capture assay (mICA).

    Siris, Sara / Gladstone, Camilla A / Guo, Yanping / Patel, Radhika / Pinder, Christopher L / Shattock, Robin J / McKay, Paul F / Langford, Paul R / Bidmos, Fadil A

    Frontiers in immunology

    2023  Volume 14, Page(s) 1184510

    Abstract: Expression cloning of fully human monoclonal antibodies (hmAbs) is seeing powerful utility in the field of vaccinology, especially for elucidating vaccine-induced B-cell responses and novel vaccine candidate antigen discovery. Precision of the hmAb ... ...

    Abstract Expression cloning of fully human monoclonal antibodies (hmAbs) is seeing powerful utility in the field of vaccinology, especially for elucidating vaccine-induced B-cell responses and novel vaccine candidate antigen discovery. Precision of the hmAb cloning process relies on efficient isolation of hmAb-producing plasmablasts of interest. Previously, a novel immunoglobulin-capture assay (ICA) was developed, using single protein vaccine antigens, to enhance the pathogen-specific hmAb cloning output. Here, we report a novel modification of this single-antigen ICA using formalin-treated, fluorescently stained whole cell suspensions of the human bacterial invasive pathogens,
    MeSH term(s) Humans ; Antibodies, Monoclonal ; Suspensions ; Bacterial Vaccines ; Pneumococcal Vaccines ; Streptococcus pneumoniae/genetics ; Meningococcal Vaccines ; Antigens, Bacterial/genetics ; Cloning, Molecular
    Chemical Substances Antibodies, Monoclonal ; Suspensions ; Bacterial Vaccines ; Pneumococcal Vaccines ; Meningococcal Vaccines ; Antigens, Bacterial
    Language English
    Publishing date 2023-06-02
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2023.1184510
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Chlamydia trachomatis: Cell biology, immunology and vaccination

    Murray, Sam M / McKay, Paul F

    Vaccine. 2021 May 21, v. 39, no. 22

    2021  

    Abstract: Chlamydia trachomatis is the causative agent of a highly prevalent sexually transmitted bacterial disease and is associated with a number of severe disease complications. Current therapy options are successful at treating disease, but patients are left ... ...

    Abstract Chlamydia trachomatis is the causative agent of a highly prevalent sexually transmitted bacterial disease and is associated with a number of severe disease complications. Current therapy options are successful at treating disease, but patients are left without protective immunity and do not benefit the majority asymptomatic patients who do not seek treatment. As such, there is a clear need for a broad acting, protective vaccine that can prevent transmission and protect against symptomatic disease presentation. There are three key elements that underlie successful vaccine development: 1) Chlamydia biology and immune-evasion adaptations, 2) the correlates of protection that prevent disease in natural and experimental infection, 3) reflection upon the evidence provided by previous vaccine attempts. In this review, we give an overview of the unique intra-cellular biology of C. trachomatis and give insight into the dynamic combination of adaptations that allow Chlamydia to subvert host immunity and survive within the cell. We explore the current understanding of chlamydial immunity in animal models and in humans and characterise the key immune correlates of protection against infection. We discuss in detail the specific immune interactions involved in protection, with relevance placed on the CD4+ T lymphocyte and B lymphocyte responses that are key to pathogen clearance. Finally, we provide a timeline of C. trachomatis vaccine research to date and evaluate the successes and failures in development so far. With insight from these three key elements of research, we suggest potential solutions for chlamydial vaccine development and promising avenues for further exploration.
    Keywords B-lymphocytes ; Chlamydia trachomatis ; T-lymphocytes ; disease severity ; immune evasion ; immunity ; pathogens ; vaccination ; vaccine development ; vaccines
    Language English
    Dates of publication 2021-0521
    Size p. 2965-2975.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 605674-x
    ISSN 1873-2518 ; 0264-410X
    ISSN (online) 1873-2518
    ISSN 0264-410X
    DOI 10.1016/j.vaccine.2021.03.043
    Database NAL-Catalogue (AGRICOLA)

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  10. Article ; Online: Use of Chlamydial Elementary Bodies as Probes to Isolate Pathogen-Specific Human Monoclonal Antibodies.

    Pinder, Christopher L / McKay, Paul F / Shattock, Robin J

    Methods in molecular biology (Clifton, N.J.)

    2020  Volume 2183, Page(s) 19–28

    Abstract: Chlamydia trachomatis is one of the most prevalent sexually transmitted infectious agents in the world and the leading cause of infectious blindness. The role of antibodies in the prevention and clearance of infection is still not fully understood, but ... ...

    Abstract Chlamydia trachomatis is one of the most prevalent sexually transmitted infectious agents in the world and the leading cause of infectious blindness. The role of antibodies in the prevention and clearance of infection is still not fully understood, but the analysis of the immunoglobulin response to novel vaccine candidates is an important part of many of these studies. In this chapter, we describe a novel method to identify and isolate Chlamydia-specific memory B cells by fluorescence-activated cell sorting (FACS) using fluorescently labeled whole bacteria from cryopreserved human PBMC samples. This method allows for live single cells to be sorted for cell culture, in vitro assays, single-cell RNA sequencing, and cloning of paired heavy and light chains for recombinant monoclonal antibody production.
    MeSH term(s) Antibodies, Bacterial/immunology ; Antibodies, Monoclonal/immunology ; Antibodies, Monoclonal/isolation & purification ; Antibody Specificity/immunology ; Antigens, Bacterial/immunology ; B-Lymphocytes/immunology ; B-Lymphocytes/metabolism ; Bacterial Vaccines/immunology ; Chlamydia/immunology ; Cryopreservation ; Humans ; Immunoglobulin G/immunology ; Immunoglobulin G/isolation & purification ; Immunologic Memory ; Leukocytes, Mononuclear/immunology ; Leukocytes, Mononuclear/metabolism ; Molecular Probes
    Chemical Substances Antibodies, Bacterial ; Antibodies, Monoclonal ; Antigens, Bacterial ; Bacterial Vaccines ; Immunoglobulin G ; Molecular Probes
    Language English
    Publishing date 2020-09-21
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-0795-4_3
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