LIVIVO - Search results -

Search results

Result 1 - 10 of total 318

Search options

Article ; Online: Investigating the Sensitivity of Nasal or Throat Swabs: Combination of Both Swabs Increases the Sensitivity of SARS-CoV-2 Rapid Antigen Tests.

Goodall, Barbara L / LeBlanc, Jason J / Hatchette, Todd F / Barrett, Lisa / Patriquin, Glenn

2022 Volume 10, Issue 4, Page(s) e0021722

Abstract: The COVID-19 pandemic has been hallmarked by several waves of variants of concern (VoCs), each with novel challenges. Currently, the highly transmissible Omicron VoC is predominant worldwide, and sore throat is common, among other cold-like symptoms. ... ...

Abstract	The COVID-19 pandemic has been hallmarked by several waves of variants of concern (VoCs), each with novel challenges. Currently, the highly transmissible Omicron VoC is predominant worldwide, and sore throat is common, among other cold-like symptoms. Anecdotes on social media have suggested that sampling one's throat can increase the sensitivity for Omicron detection by antigen-based rapid testing devices (Ag-RDTs). This work aimed to improve the local testing strategy and determine whether the sensitivity of Ag-RDTs designed for nasal sampling is altered with the use of self-administered throat swabs in self-perceived asymptomatic individuals. This investigation used a common Ag-RDT (i.e., Abbott Panbio COVID-19 Ag rapid test device) to compare three sampling sites: nasal swab, throat swab, and combined nasal/throat. All Ag-RDT results were confirmed with molecular testing from residual test buffer. Compared to reverse transcriptase PCR (RT-PCR), samples from nasal or throat swabs each detected 64.5% of SARS-CoV-2 cases; however, combining the contributions of each swab increased the positive percent agreement (PPA) with RT-PCR to 88.7%. This trend was also evident with the Rapid Response Ag-RDT (BTNX), which uses more flexible swabs than does the Panbio. When nasal swab collection was compared to paired sampling of the nose/throat using a single swab with the Panbio Ag-RDT, the PPAs were 68.4% and 81.6%, respectively. No false-positive results were observed with nasal, throat, or combined nasal/throat sampling. Self-administered throat and nasal/throat swabs both had >90% acceptability. These findings support the use of self-collected combined nasal/throat sampling for Ag-RDT-based SARS-CoV-2 detection in self-perceived asymptomatic individuals.
MeSH term(s)	COVID-19/diagnosis ; Humans ; Pandemics ; Pharynx ; SARS-CoV-2 ; Sensitivity and Specificity
Language	English
Publishing date	2022-06-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.00217-22
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Self-limited gastrointestinal bleeding in COVID-19.

Barrett, Lisa F / Lo, Kevin Bryan / Stanek, Steven R / Walter, James W

Clinics and research in hepatology and gastroenterology

2020 Volume 44, Issue 4, Page(s) e77–e80

MeSH term(s)	Aged ; Betacoronavirus ; COVID-19 ; Coronavirus Infections/blood ; Coronavirus Infections/complications ; Female ; Gastrointestinal Hemorrhage/blood ; Gastrointestinal Hemorrhage/etiology ; Hemoglobin A/analysis ; Humans ; Male ; Melena/etiology ; Pandemics ; Pneumonia, Viral/blood ; Pneumonia, Viral/complications ; Retrospective Studies ; SARS-CoV-2
Chemical Substances	Hemoglobin A (9034-51-9)
Keywords	covid19
Language	English
Publishing date	2020-07-15
Publishing country	France
Document type	Case Reports ; Letter
ZDB-ID	2594333-9
ISSN	2210-741X ; 2210-7401
ISSN (online)	2210-741X
ISSN	2210-7401
DOI	10.1016/j.clinre.2020.06.015
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Taqman PACMAN: a simple molecular approach for positive rapid antigen test confirmation during periods of low prevalence.

McCracken, Gregory R / Patriquin, Glenn / Hatchette, Todd F / Davidson, Ross J / Goodall, Barbara / Barrett, Lisa / MacDonald, James / Heinstein, Charles / Pettipas, Janice / Ross, John / LeBlanc, Jason J

Microbiology spectrum

2024 Volume 12, Issue 5, Page(s) e0407323

Abstract: Antigen-based rapid diagnostic tests (Ag-RDTs) were widely deployed to enhance SARS-CoV-2 testing capacity during the COVID-19 pandemic. Consistent with national guidance for low prevalence settings, positive Ag-RDTs were confirmed using nucleic acid ... ...

Abstract	Antigen-based rapid diagnostic tests (Ag-RDTs) were widely deployed to enhance SARS-CoV-2 testing capacity during the COVID-19 pandemic. Consistent with national guidance for low prevalence settings, positive Ag-RDTs were confirmed using nucleic acid amplification tests (NAATs) to avoid false positive results. However, increasing demands for positive Ag-RDT confirmation competed with other testing priorities in clinical laboratories. This work hypothesized that real-time RT-PCR without nucleic acid extraction (NAE) would be sufficiently sensitive to support positive Ag-RDT confirmation. Ag-RDT and NAAT results from community-based asymptomatic testing sites prior to the omicron variant wave were compared to calculate the weekly false positive rate (FPR) and false detection rate (FDR). Real-time RT-PCR was compared with and without NAE using 752 specimens previously tested positive for SARS-CoV-2 using commercial NAATs and 344 specimens from Ag-RDT-positive individuals. The impact of SARS-CoV-2 prevalence on laboratory resources required to sustain Ag-RDT confirmation was modeled for the RT-PCR with and without NAE. Overall, FPR was low [0.07% (222/330,763)] in asymptomatic testing sites, but FDR was high [30.7% (222/724)]. When RT-PCR was compared with and without NAE, 100% concordance was obtained with NAAT-positive specimens, including those from Ag-RDT-positive individuals. NAE-free RT-PCR significantly reduced time to results, human resources, and overall costs. A 30.7% FDR reaffirms the need for NAAT-based confirmation of positive Ag-RDT results during low SARS-CoV-2 prevalence. NAE-free RT-PCR was shown to be a simple and cost-sparing NAAT-based solution for positive Ag-RDT confirmation, and its implementation supported data-driven broader Ag-RDT deployment into communities, workplaces, and households. Importance: Rapid antigen testing for SARS-CoV-2 was widely deployed during the COVID-19 pandemic. In settings of low prevalence, national guidance recommends that positive antigen test results be confirmed with molecular testing. Given the high testing burden on clinical laboratories during the COVID-19 pandemic, the high volume of positive antigen tests submitted for confirmatory testing posed challenges for laboratory workflow. This study demonstrated that a simple PCR method without prior nucleic acid purification is an accurate and cost-effective solution for positive rapid antigen test confirmation. Implementing this method allowed molecular confirmatory testing for positive antigen tests to be sustained as antigen testing was expanded into large populations such as workplaces, schools, and households.
MeSH term(s)	Humans ; COVID-19/diagnosis ; COVID-19/epidemiology ; SARS-CoV-2/genetics ; SARS-CoV-2/immunology ; SARS-CoV-2/isolation & purification ; Antigens, Viral ; Sensitivity and Specificity ; Prevalence ; False Positive Reactions ; COVID-19 Serological Testing/methods ; COVID-19 Nucleic Acid Testing/methods ; Nucleic Acid Amplification Techniques/methods ; Real-Time Polymerase Chain Reaction/methods
Chemical Substances	Antigens, Viral
Language	English
Publishing date	2024-04-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.04073-23
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Infection of a prosthetic knee joint with

Barrett, Lisa F / Saragadam, Sailaja Devi / DiMaria, Christina N / Delgado-Daza, Alejandro

Oxford medical case reports

2020 Volume 2020, Issue 8, Page(s) omaa057

Abstract: We present the first reported case of prosthetic joint infection caused ... ...

Abstract	We present the first reported case of prosthetic joint infection caused by
Language	English
Publishing date	2020-08-10
Publishing country	England
Document type	Case Reports
ZDB-ID	2766251-2
ISSN	2053-8855
ISSN	2053-8855
DOI	10.1093/omcr/omaa057
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Genomic landscape of diploid and aneuploid microsatellite stable early onset colorectal cancer.

Zhou, Yumei / Chen, Xianfeng / Chen, Jun / Kendrick, Conner D / Ramanathan, Ramesh K / Graham, Rondell P / Kossick, Kimberlee F / Boardman, Lisa A / Barrett, Michael T

Scientific reports

2024 Volume 14, Issue 1, Page(s) 9368

Abstract: Although colorectal cancer (CRC) remains the second leading cause of cancer-related death in the United States, the overall incidence and mortality from the disease have declined in recent decades. In contrast, there has been a steady increase in the ... ...

Abstract	Although colorectal cancer (CRC) remains the second leading cause of cancer-related death in the United States, the overall incidence and mortality from the disease have declined in recent decades. In contrast, there has been a steady increase in the incidence of CRC in individuals under 50 years of age. Hereditary syndromes contribute disproportionately to early onset CRC (EOCRC). These include microsatellite instability high (MSI+) tumors arising in patients with Lynch Syndrome. However, most EOCRCs are not associated with familial syndromes or MSI+ genotypes. Comprehensive genomic profiling has provided the basis of improved more personalized treatments for older CRC patients. However, less is known about the basis of sporadic EOCRC. To define the genomic landscape of EOCRC we used DNA content flow sorting to isolate diploid and aneuploid tumor fractions from 21 non-hereditary cases. We then generated whole exome mutational profiles for each case and whole genome copy number, telomere length, and EGFR immunohistochemistry (IHC) analyses on subsets of samples. These results discriminate the molecular features of diploid and aneuploid EOCRC and provide a basis for larger population-based studies and the development of effective strategies to monitor and treat this emerging disease.
MeSH term(s)	Humans ; Aneuploidy ; Colorectal Neoplasms/genetics ; Diploidy ; Microsatellite Instability ; Middle Aged ; Female ; Male ; Adult ; Mutation ; ErbB Receptors/genetics ; Age of Onset ; Genomics/methods
Chemical Substances	ErbB Receptors (EC 2.7.10.1) ; EGFR protein, human (EC 2.7.10.1)
Language	English
Publishing date	2024-04-23
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-59398-5
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Improving the study of brain-behavior relationships by revisiting basic assumptions.

Westlin, Christiana / Theriault, Jordan E / Katsumi, Yuta / Nieto-Castanon, Alfonso / Kucyi, Aaron / Ruf, Sebastian F / Brown, Sarah M / Pavel, Misha / Erdogmus, Deniz / Brooks, Dana H / Quigley, Karen S / Whitfield-Gabrieli, Susan / Barrett, Lisa Feldman

Trends in cognitive sciences

2023 Volume 27, Issue 3, Page(s) 246–257

Abstract: Neuroimaging research has been at the forefront of concerns regarding the failure of experimental findings to replicate. In the study of brain-behavior relationships, past failures to find replicable and robust effects have been attributed to ... ...

Abstract	Neuroimaging research has been at the forefront of concerns regarding the failure of experimental findings to replicate. In the study of brain-behavior relationships, past failures to find replicable and robust effects have been attributed to methodological shortcomings. Methodological rigor is important, but there are other overlooked possibilities: most published studies share three foundational assumptions, often implicitly, that may be faulty. In this paper, we consider the empirical evidence from human brain imaging and the study of non-human animals that calls each foundational assumption into question. We then consider the opportunities for a robust science of brain-behavior relationships that await if scientists ground their research efforts in revised assumptions supported by current empirical evidence.
MeSH term(s)	Animals ; Humans ; Brain/diagnostic imaging ; Neuroimaging/methods
Language	English
Publishing date	2023-02-02
Publishing country	England
Document type	Journal Article ; Review ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2010989-1
ISSN	1879-307X ; 1364-6613
ISSN (online)	1879-307X
ISSN	1364-6613
DOI	10.1016/j.tics.2022.12.015
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Self-limited gastrointestinal bleeding in COVID-19

Barrett, Lisa F / Lo, Kevin Bryan / Stanek, Steven R / Walter, James W

Clin Res Hepatol Gastroenterol

Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #645711
Database	COVID19

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Self-limited gastrointestinal bleeding in COVID-19

Barrett, Lisa F. / Lo, Kevin Bryan / Stanek, Steven R. / Walter, James W.

Clinics and Research in Hepatology and Gastroenterology

2020 Volume 44, Issue 4, Page(s) e77–e80

Keywords	Gastroenterology ; covid19
Language	English
Publisher	Elsevier BV
Publishing country	us
Document type	Article ; Online
ZDB-ID	2594333-9
ISSN	2210-741X ; 2210-7401
ISSN (online)	2210-741X
ISSN	2210-7401
DOI	10.1016/j.clinre.2020.06.015
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Order via subito

Details ▾
- Full text online
- Order with fees

Article ; Online: Comparison between Nasal and Nasopharyngeal Swabs for SARS-CoV-2 Rapid Antigen Detection in an Asymptomatic Population, and Direct Confirmation by RT-PCR from the Residual Buffer.

Patriquin, Glenn / LeBlanc, Jason J / Williams, Catherine / Hatchette, Todd F / Ross, John / Barrett, Lisa / Davidson, Ross

Microbiology spectrum

2022 Volume 10, Issue 1, Page(s) e0245521

Abstract: Containment measures employed during the COVID-19 pandemic included prompt recognition of cases, isolation, and contact tracing. Bilateral nasal (NA) swabs applied to a commercial antigen-based rapid diagnostic test (Ag-RDT) offer a simpler and more ... ...

Abstract	Containment measures employed during the COVID-19 pandemic included prompt recognition of cases, isolation, and contact tracing. Bilateral nasal (NA) swabs applied to a commercial antigen-based rapid diagnostic test (Ag-RDT) offer a simpler and more comfortable alternative to nasopharyngeal (NP) collection; however, little is known about the sensitivity of this method in an asymptomatic population. Participants in community-based asymptomatic testing sites were screened for SARS-CoV-2 using an Ag-RDT with NP sampling. Positive individuals returned for confirmatory molecular testing and consented to repeating the Ag-RDT using a bilateral NA swab for comparison. Residual test buffer (RTB) from Ag-RDTs was subjected to real-time reverse transcription-PCR (RT-PCR). Of 123,617 asymptomatic individuals, 197 NP Ag-RDT-positive participants were included, with 175 confirmed positive by RT-PCR. Of these cases, 154 were identified from the NA swab collection with Ag-RDT, with a sensitivity of 88.0% compared to the NP swab collection. Stratifying results by RT-PCR cycle threshold demonstrated that sensitivity of the nasal collection method varied based on the cycle threshold (
MeSH term(s)	Antigens, Viral/analysis ; Antigens, Viral/genetics ; Antigens, Viral/immunology ; Asymptomatic Diseases ; COVID-19/diagnosis ; COVID-19/virology ; COVID-19 Serological Testing ; Carrier State/virology ; Humans ; Nasopharynx/virology ; Nose/virology ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2/classification ; SARS-CoV-2/genetics ; SARS-CoV-2/immunology ; SARS-CoV-2/isolation & purification ; Sensitivity and Specificity ; Specimen Handling/methods
Chemical Substances	Antigens, Viral
Language	English
Publishing date	2022-02-16
Publishing country	United States
Document type	Comparative Study ; Journal Article
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.02455-21
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Avoiding False-Positive SARS-CoV-2 Rapid Antigen Test Results with Point-of-Care Molecular Testing on Residual Test Buffer.

LeBlanc, Jason J / McCracken, Gregory R / Goodall, Barbara / Hatchette, Todd F / Barrett, Lisa / Ross, John / Davidson, Ross J / Patriquin, Glenn

Microbiology spectrum

2022 Volume 10, Issue 4, Page(s) e0063922

Abstract: Antigen-based rapid diagnostic tests (Ag-RDTs) have been widely used for the detection of SARS-CoV-2 during the coronavirus disease 2019 (COVID-19) pandemic. In settings of low disease prevalence, such as asymptomatic community testing, national ... ...

Abstract	Antigen-based rapid diagnostic tests (Ag-RDTs) have been widely used for the detection of SARS-CoV-2 during the coronavirus disease 2019 (COVID-19) pandemic. In settings of low disease prevalence, such as asymptomatic community testing, national guidelines recommend confirmation of positive Ag-RDT results with a nucleic acid amplification test (NAAT). This often requires patients to be recalled for repeat specimen recollection and subsequent testing in reference laboratories. This project assessed the use of a point-of-care molecular NAAT for SARS-CoV-2 detection (i.e., ID NOW), which was performed on-site at a volunteer-led asymptomatic community testing site on the residual test buffer (RTB) from positive Ag-RDTs. The ID NOW NAAT assay was performed on RTB from two Ag-RDTs: the Abbott Panbio and BTNX Rapid Response assays. Results of ID NOW were compared to real-time RT-PCR at a reference laboratory. Along with investigations into the clinical performance of ID NOW on RTB, analytical specificity was assessed with a panel of various respiratory organisms. Of the Ag-RDTs results evaluated, all 354 Ag-RDTs results characterized as true positives by RT-PCR were accurately identified with ID NOW testing of RTB. No SARS-CoV-2 detections by ID NOW were observed from 10 specimens characterized as false-positive Ag-RDTs, or from contrived specimens with various respiratory organisms. The use of on-site molecular testing on RTB provides a suitable option for rapid confirmatory testing of positive Ag-RDTs, thereby obviating the need for specimen recollection for molecular testing at local reference laboratories.
MeSH term(s)	COVID-19/diagnosis ; Humans ; Molecular Diagnostic Techniques/methods ; Pandemics ; Point-of-Care Systems ; SARS-CoV-2/genetics ; Sensitivity and Specificity
Language	English
Publishing date	2022-07-13
Publishing country	United States
Document type	Journal Article
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.00639-22
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito