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  1. Article ; Online: 16S rRNA sequencing-based evaluation of the protective effects of Hua-Zhuo-Jie-Du on rats with chronic atrophic gastritis.

    Zhou, Pingping / Yang, Tianxiao / Xu, Miaochan / Zhao, Yuejia / Shen, Pengpeng / Wang, Yangang

    BMC complementary medicine and therapies

    2022  Volume 22, Issue 1, Page(s) 71

    Abstract: ... atrophic gastritis (CAG). Hua-Zhuo-Jie-Du (HZJD) has been shown to be an effective Chinese herbal preparation ...

    Abstract Background: Disturbance of the intestinal flora is a pathogenic factor for chronic atrophic gastritis (CAG). Hua-Zhuo-Jie-Du (HZJD) has been shown to be an effective Chinese herbal preparation for treating CAG. However, the effects of HZJD on the intestinal flora of CAG is unclear. In this study, we probed the regulating effects of HZJD on intestinal microbes in CAG rats using 16S rRNA gene sequencing.
    Methods: High-performance liquid chromatography (HPLC) analysis was used to perform quality control of HZJD preparations. We then administered 1-methyl-3-nitro-1-nitrosoguanidine (200 μg/ml) to Sprague-Dawley rats to establish a CAG model. HZJD and vitacoenzyme were administered orally to these rats over a 10 week period. Hematoxylin and eosin (H&E) staining was performed to observe the histopathology of CAG rats. A rarefaction curve, species accumulation curve, Chao1 index, and ACE index were calculated to assess the alpha diversity. Principal component analysis (PCA), non-metric multi-dimensional scaling (NMDS), and unweighted pair group method with arithmetic mean (UPGMA) were conducted to examine the beta diversity. The LEfSe method was used to identify differential bacteria. Differential function analysis used PCA based on KEGG function prediction.
    Results: HPLC showed that our HZJD preparation method was feasible. H&E staining showed that HZJD significantly improved the pathological state of the gastric mucosa in CAG rats. The rarefaction curve and species accumulation curve showed that the sequencing data were reasonable. The Chao1 and ACE indices were significantly increased in CAG rats compared to the N group. Following HZJD and vitacoenzyme treatment, the Chao1 and ACE indices were decreased. PCA, NMDS, and UPGMA results showed that the M group was separated from the N, HZJD, and V groups, and LEfSe results showed that the relative abundance of Akkermansia, Oscillospira, Prevotella, and CF231 were significantly higher in the N group. Proteobacteria and Escherichia were significantly enriched in the M group, Allobaculum, Bacteroides, Jeotgalicoccus, Corynebacterium, and Sporosarcina were significantly enriched in the V group, and Firmicutes, Lactobacillus, and Turicibacter were significantly enriched in the HZJD group.
    Conclusion: HZJD exhibited a therapeutic effect on the intestinal flora of CAG rats.
    MeSH term(s) Animals ; Gastric Mucosa ; Gastritis, Atrophic/drug therapy ; Gastritis, Atrophic/pathology ; Gastrointestinal Microbiome ; RNA, Ribosomal, 16S/genetics ; Rats ; Rats, Sprague-Dawley
    Chemical Substances RNA, Ribosomal, 16S
    Language English
    Publishing date 2022-03-16
    Publishing country England
    Document type Journal Article
    ISSN 2662-7671
    ISSN (online) 2662-7671
    DOI 10.1186/s12906-022-03542-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: 16S rRNA sequencing-based evaluation of the protective effects of Hua-Zhuo-Jie-Du on rats with chronic atrophic gastritis

    Zhou, Pingping / Yang, Tianxiao / Xu, Miaochan / Zhao, Yuejia / Shen, Pengpeng / Wang, Yangang

    BMC Complement Med Ther. 2022 Dec., v. 22, no. 1 p.71-71

    2022  

    Abstract: ... atrophic gastritis (CAG). Hua-Zhuo-Jie-Du (HZJD) has been shown to be an effective Chinese herbal preparation ...

    Abstract BACKGROUND: Disturbance of the intestinal flora is a pathogenic factor for chronic atrophic gastritis (CAG). Hua-Zhuo-Jie-Du (HZJD) has been shown to be an effective Chinese herbal preparation for treating CAG. However, the effects of HZJD on the intestinal flora of CAG is unclear. In this study, we probed the regulating effects of HZJD on intestinal microbes in CAG rats using 16S rRNA gene sequencing. METHODS: High-performance liquid chromatography (HPLC) analysis was used to perform quality control of HZJD preparations. We then administered 1-methyl-3-nitro-1-nitrosoguanidine (200 μg/ml) to Sprague–Dawley rats to establish a CAG model. HZJD and vitacoenzyme were administered orally to these rats over a 10 week period. Hematoxylin and eosin (H&E) staining was performed to observe the histopathology of CAG rats. A rarefaction curve, species accumulation curve, Chao1 index, and ACE index were calculated to assess the alpha diversity. Principal component analysis (PCA), non-metric multi-dimensional scaling (NMDS), and unweighted pair group method with arithmetic mean (UPGMA) were conducted to examine the beta diversity. The LEfSe method was used to identify differential bacteria. Differential function analysis used PCA based on KEGG function prediction. RESULTS: HPLC showed that our HZJD preparation method was feasible. H&E staining showed that HZJD significantly improved the pathological state of the gastric mucosa in CAG rats. The rarefaction curve and species accumulation curve showed that the sequencing data were reasonable. The Chao1 and ACE indices were significantly increased in CAG rats compared to the N group. Following HZJD and vitacoenzyme treatment, the Chao1 and ACE indices were decreased. PCA, NMDS, and UPGMA results showed that the M group was separated from the N, HZJD, and V groups, and LEfSe results showed that the relative abundance of Akkermansia, Oscillospira, Prevotella, and CF231 were significantly higher in the N group. Proteobacteria and Escherichia were significantly enriched in the M group, Allobaculum, Bacteroides, Jeotgalicoccus, Corynebacterium, and Sporosarcina were significantly enriched in the V group, and Firmicutes, Lactobacillus, and Turicibacter were significantly enriched in the HZJD group. CONCLUSION: HZJD exhibited a therapeutic effect on the intestinal flora of CAG rats.
    Keywords Bacteroides ; Corynebacterium ; Escherichia ; Jeotgalicoccus ; Lactobacillus ; Oscillospira ; Prevotella ; Sporosarcina ; complement ; eosin ; gastric mucosa ; gastritis ; genes ; high performance liquid chromatography ; histopathology ; intestinal microorganisms ; intestines ; models ; pathological processes and conditions ; prediction ; principal component analysis ; quality control ; species diversity ; therapeutics
    Language English
    Dates of publication 2022-12
    Size p. 71.
    Publishing place BioMed Central
    Document type Article ; Online
    ISSN 2662-7671
    DOI 10.1186/s12906-022-03542-z
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Determination of the protective effects of Hua‐Zhuo‐Jie‐Du in chronic atrophic gastritis by regulating intestinal microbiota and metabolites

    Pingping Zhou / Xinyu Hao / Yu Liu / Zeqi Yang / Miaochan Xu / Shaowei Liu / Shixiong Zhang / Tianxiao Yang / Xiaomei Wang / Yangang Wang

    Chinese Medicine, Vol 16, Iss 1, Pp 1-

    combination of liquid chromatograph mass spectrometer metabolic profiling and 16S rRNA gene sequencing

    2021  Volume 19

    Abstract: Abstract Background Hua-Zhuo-Jie-Du (HZJD), a Chinese herbal prescription consisting of 11 herbs ...

    Abstract Abstract Background Hua-Zhuo-Jie-Du (HZJD), a Chinese herbal prescription consisting of 11 herbs, is commonly used in China to treat chronic atrophic gastritis (CAG). We aimed to determine the effect of HZJD on the microbiome-associated metabolic changes in CAG rats. Methods The CAG rat models were induced by 1-methyl-3-nitro-1-nitrosoguanidine (MNNG) combined with irregular fasting and 2% sodium salicylate, which was intragastrically administrated in fasted animals for 24 weeks. The CAG rats in the Chinese medicine (CM) group were administered a daily dose of 14.81 g/kg/day HZJD, and the vitacoenzyme (V) group were administered a daily dose of 0.08 g/kg/day vitacoenzyme. All animals were treated for 10 consecutive weeks, consecutively. Hematoxylin and eosin (H&E) staining was used to assess the histopathological changes in the gastric tissues. An integrated approach based on liquid chromatograph mass spectrometer (LC-MS) metabolic profiling combined with 16S rRNA gene sequencing was carried out to assess the effects of HZJD on CAG rats. Spearman analysis was used to calculate the correlation coefficient between the different intestinal microbiota and the metabolites. Results The H&E results indicated that HZJD could improve the pathological condition of CAG rats. The LC–MS results indicated that HZJD could significantly improve 21 gastric mucosal tissue perturbed metabolites in CAG rats; the affected metabolites were found to be involved in multiple metabolic pathways, such as the central carbon metabolism in cancer. The results of 16S rRNA gene sequencing indicated that HZJD could regulate the diversity, microbial composition, and abundance of the intestinal microbiota of CAG rats. Following HZJD treatment, the relative abundance of Turicibacter was increased, and the relative abundance of Desulfococcus and Escherichia were decreased in the CM group when compared with the M group. Spearman analysis revealed that perturbed intestinal microbes had a strong correlation with differential metabolites, ...
    Keywords Hua‐Zhuo‐Jie‐Du ; Chronic atrophic gastritis ; LC–MS ; 16S rRNA gene sequencing ; Other systems of medicine ; RZ201-999
    Subject code 610
    Language English
    Publishing date 2021-05-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article: Determination of the protective effects of Hua-Zhuo-Jie-Du in chronic atrophic gastritis by regulating intestinal microbiota and metabolites: combination of liquid chromatograph mass spectrometer metabolic profiling and 16S rRNA gene sequencing.

    Zhou, Pingping / Hao, Xinyu / Liu, Yu / Yang, Zeqi / Xu, Miaochan / Liu, Shaowei / Zhang, Shixiong / Yang, Tianxiao / Wang, Xiaomei / Wang, Yangang

    Chinese medicine

    2021  Volume 16, Issue 1, Page(s) 37

    Abstract: Background: Hua-Zhuo-Jie-Du (HZJD), a Chinese herbal prescription consisting of 11 herbs, is ...

    Abstract Background: Hua-Zhuo-Jie-Du (HZJD), a Chinese herbal prescription consisting of 11 herbs, is commonly used in China to treat chronic atrophic gastritis (CAG). We aimed to determine the effect of HZJD on the microbiome-associated metabolic changes in CAG rats.
    Methods: The CAG rat models were induced by 1-methyl-3-nitro-1-nitrosoguanidine (MNNG) combined with irregular fasting and 2% sodium salicylate, which was intragastrically administrated in fasted animals for 24 weeks. The CAG rats in the Chinese medicine (CM) group were administered a daily dose of 14.81 g/kg/day HZJD, and the vitacoenzyme (V) group were administered a daily dose of 0.08 g/kg/day vitacoenzyme. All animals were treated for 10 consecutive weeks, consecutively. Hematoxylin and eosin (H&E) staining was used to assess the histopathological changes in the gastric tissues. An integrated approach based on liquid chromatograph mass spectrometer (LC-MS) metabolic profiling combined with 16S rRNA gene sequencing was carried out to assess the effects of HZJD on CAG rats. Spearman analysis was used to calculate the correlation coefficient between the different intestinal microbiota and the metabolites.
    Results: The H&E results indicated that HZJD could improve the pathological condition of CAG rats. The LC-MS results indicated that HZJD could significantly improve 21 gastric mucosal tissue perturbed metabolites in CAG rats; the affected metabolites were found to be involved in multiple metabolic pathways, such as the central carbon metabolism in cancer. The results of 16S rRNA gene sequencing indicated that HZJD could regulate the diversity, microbial composition, and abundance of the intestinal microbiota of CAG rats. Following HZJD treatment, the relative abundance of Turicibacter was increased, and the relative abundance of Desulfococcus and Escherichia were decreased in the CM group when compared with the M group. Spearman analysis revealed that perturbed intestinal microbes had a strong correlation with differential metabolites, Escherichia exhibited a negative correlation with l-Leucine, Turicibacter was negatively correlated with urea, and Desulfococcus exhibited a positive correlation with trimethylamine, and a negative correlation with choline.
    Conclusions: HZJD could protect CAG by regulating intestinal microbiota and its metabolites.
    Language English
    Publishing date 2021-05-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 2260322-0
    ISSN 1749-8546
    ISSN 1749-8546
    DOI 10.1186/s13020-021-00445-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Book: Zai pi pi shu jia zhuo mi cang

    Zhou, Jing / Hu, Ningna

    (Wo xi huan qu wei han zi you xi shu ; 我喜欢趣味汉字游戏书)

    2006  

    Author's details Zhou Jing zhu Hu Ningna hui
    Series title Wo xi huan qu wei han zi you xi shu
    我喜欢趣味汉字游戏书
    Language Chinese
    Size 30 S., ill
    Publisher Jiangsu shao nian er tong chu ban she
    Publishing place Nanjing
    Document type Book
    ISBN 9787534637001 ; 7534637007
    Database Former special subject collection: coastal and deep sea fishing

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  6. Article: Acute bacterial encephalitis complicated with recurrent nasopharyngeal carcinoma associated with

    Zhuo, Xiaohuang / Zhou, Yongzhao / Liu, Ling

    Frontiers in neurology

    2023  Volume 13, Page(s) 965939

    Abstract: Elizabethkingia ... ...

    Abstract Elizabethkingia miricola
    Language English
    Publishing date 2023-01-27
    Publishing country Switzerland
    Document type Case Reports
    ZDB-ID 2564214-5
    ISSN 1664-2295
    ISSN 1664-2295
    DOI 10.3389/fneur.2022.965939
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Joint deep autoencoder and subgraph augmentation for inferring microbial responses to drugs.

    Zhou, Zhecheng / Zhuo, Linlin / Fu, Xiangzheng / Zou, Quan

    Briefings in bioinformatics

    2024  Volume 25, Issue 1

    Abstract: Exploring microbial stress responses to drugs is crucial for the advancement of new therapeutic methods. While current artificial intelligence methodologies have expedited our understanding of potential microbial responses to drugs, the models are ... ...

    Abstract Exploring microbial stress responses to drugs is crucial for the advancement of new therapeutic methods. While current artificial intelligence methodologies have expedited our understanding of potential microbial responses to drugs, the models are constrained by the imprecise representation of microbes and drugs. To this end, we combine deep autoencoder and subgraph augmentation technology for the first time to propose a model called JDASA-MRD, which can identify the potential indistinguishable responses of microbes to drugs. In the JDASA-MRD model, we begin by feeding the established similarity matrices of microbe and drug into the deep autoencoder, enabling to extract robust initial features of both microbes and drugs. Subsequently, we employ the MinHash and HyperLogLog algorithms to account intersections and cardinality data between microbe and drug subgraphs, thus deeply extracting the multi-hop neighborhood information of nodes. Finally, by integrating the initial node features with subgraph topological information, we leverage graph neural network technology to predict the microbes' responses to drugs, offering a more effective solution to the 'over-smoothing' challenge. Comparative analyses on multiple public datasets confirm that the JDASA-MRD model's performance surpasses that of current state-of-the-art models. This research aims to offer a more profound insight into the adaptability of microbes to drugs and to furnish pivotal guidance for drug treatment strategies. Our data and code are publicly available at: https://github.com/ZZCrazy00/JDASA-MRD.
    MeSH term(s) Artificial Intelligence ; Algorithms ; Neural Networks, Computer
    Language English
    Publishing date 2024-01-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2068142-2
    ISSN 1477-4054 ; 1467-5463
    ISSN (online) 1477-4054
    ISSN 1467-5463
    DOI 10.1093/bib/bbad483
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Dinitrogen Activation and Addition to Unsaturated C-E (E=C, N, O, S) Bonds Mediated by Transition Metal Complexes.

    Zhuo, Qingde / Zhou, Xiaoxi / Shima, Takanori / Hou, Zhaomin

    Angewandte Chemie (International ed. in English)

    2023  Volume 62, Issue 19, Page(s) e202218606

    Abstract: Dinitrogen ( ... ...

    Abstract Dinitrogen (N
    Language English
    Publishing date 2023-02-20
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    DOI 10.1002/anie.202218606
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: ALKBH1

    Zhang, Xinxin / Zhou, Chunlei / Zhao, Yemu / Deng, Changmi / Wu, Haiyan / Zhuo, Zhenjian / He, Jing

    Journal of Cancer

    2024  Volume 15, Issue 2, Page(s) 526–532

    Abstract: Neuroblastoma is a highly malignant extracranial solid tumor in pediatrics. ALKBH1 as a recently discovered DNA N6-methyldeoxyadenosine (6mA) demethylase closely links to tumorigenesis. Whether ... ...

    Abstract Neuroblastoma is a highly malignant extracranial solid tumor in pediatrics. ALKBH1 as a recently discovered DNA N6-methyldeoxyadenosine (6mA) demethylase closely links to tumorigenesis. Whether the
    Language English
    Publishing date 2024-01-01
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 2573318-7
    ISSN 1837-9664
    ISSN 1837-9664
    DOI 10.7150/jca.89271
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: A rare case of benign pancreatic schwannoma with regional lymph node metastasis.

    Zhuo, Yuejian / Zhou, Xuejun / Cao, Ping / Zhang, Dongdong

    Asian journal of surgery

    2023  Volume 46, Issue 10, Page(s) 4418–4419

    MeSH term(s) Humans ; Lymphatic Metastasis/pathology ; Lymph Nodes/pathology ; Lymph Node Excision ; Neurilemmoma/diagnostic imaging ; Neurilemmoma/surgery ; Pancreatic Neoplasms/diagnostic imaging ; Pancreatic Neoplasms/surgery ; Pancreatic Neoplasms/pathology
    Language English
    Publishing date 2023-04-30
    Publishing country Netherlands
    Document type Case Reports ; Letter
    ZDB-ID 1068461-x
    ISSN 0219-3108 ; 1015-9584
    ISSN (online) 0219-3108
    ISSN 1015-9584
    DOI 10.1016/j.asjsur.2023.04.085
    Database MEDical Literature Analysis and Retrieval System OnLINE

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