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Article ; Online: Noninvasive Neuromodulation of Peripheral Nerve Pathways Using Ultrasound and Its Current Therapeutic Implications.

Puleo, Christopher / Cotero, Victoria

Cold Spring Harbor perspectives in medicine

2020 Volume 10, Issue 2

Abstract: This review describes work from several research groups in which ultrasound is being used to target the peripheral nervous system and perform neuromodulation noninvasively. Although these techniques are in their infancy compared to implant-based and ... ...

Abstract	This review describes work from several research groups in which ultrasound is being used to target the peripheral nervous system and perform neuromodulation noninvasively. Although these techniques are in their infancy compared to implant-based and electrical nerve stimulation, if successful this new noninvasive method for neuromodulation could solve many of the challenges facing the field of bioelectronic medicine. The work outlined herein shows results in which two different (potentially therapeutic) targets are stimulated, a neuroimmune pathway within the spleen and a nutrient/sensory pathway within the liver. Both data and discussion are provided that compare this new noninvasive technique to implant-based nerve stimulation.
MeSH term(s)	Afferent Pathways/immunology ; Afferent Pathways/physiology ; Animals ; Humans ; Neuroimmunomodulation/physiology ; Peripheral Nerves/physiology ; Spleen/immunology ; Spleen/innervation ; Spleen/physiology ; Ultrasonic Therapy/methods
Language	English
Publishing date	2020-02-03
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
ISSN	2157-1422
ISSN (online)	2157-1422
DOI	10.1101/cshperspect.a034215
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Accelerating cutaneous healing in a rodent model of type II diabetes utilizing non-invasive focused ultrasound targeted at the spleen.

Morton, Christine / Cotero, Victoria / Ashe, Jeffrey / Ginty, Fiona / Puleo, Christopher

Frontiers in neuroscience

2022 Volume 16, Page(s) 1039960

Abstract: Healing of wounds is delayed in Type 2 Diabetes Mellitus (T2DM), and new treatment approaches are urgently needed. Our earlier work showed that splenic pulsed focused ultrasound (pFUS) alters inflammatory cytokines in models of acute endotoxemia and ... ...

Abstract	Healing of wounds is delayed in Type 2 Diabetes Mellitus (T2DM), and new treatment approaches are urgently needed. Our earlier work showed that splenic pulsed focused ultrasound (pFUS) alters inflammatory cytokines in models of acute endotoxemia and pneumonia
Language	English
Publishing date	2022-11-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2411902-7
ISSN	1662-453X ; 1662-4548
ISSN (online)	1662-453X
ISSN	1662-4548
DOI	10.3389/fnins.2022.1039960
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Article ; Online: Factors associated with baseline and serial changes in circulating NT-proBNP and high-sensitivity cardiac troponin T in a population-based cohort (Dallas Heart Study).

Puleo, Christopher W / Ayers, Colby R / Garg, Sonia / Neeland, Ian J / Lewis, Alana A / Pandey, Ambarish / Drazner, Mark H / de Lemos, James A

Biomarkers in medicine

2021 Volume 15, Issue 16, Page(s) 1487–1498

Abstract: Aim: ...

Abstract	Aim:
MeSH term(s)	Adult ; Aged ; Biomarkers/blood ; Female ; Heart Failure/blood ; Humans ; Male ; Middle Aged ; Natriuretic Peptide, Brain/blood ; Peptide Fragments/blood ; Risk Factors ; Troponin T/blood
Chemical Substances	Biomarkers ; Peptide Fragments ; Troponin T ; pro-brain natriuretic peptide (1-76) ; Natriuretic Peptide, Brain (114471-18-0)
Language	English
Publishing date	2021-10-19
Publishing country	England
Document type	Clinical Trial, Phase I ; Clinical Trial, Phase II ; Journal Article ; Multicenter Study ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2481014-9
ISSN	1752-0371 ; 1752-0363
ISSN (online)	1752-0371
ISSN	1752-0363
DOI	10.2217/bmm-2021-0055
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Article: Rapid Minimum Inhibitory Concentration (MIC) Analysis Using Lyophilized Reagent Beads in a Novel Multiphase, Single-Vessel Assay.

Khire, Tejas Suresh / Gao, Wei / Bales, Brian / Hsieh, Kuangwen / Grossmann, Greg / Park, Dong Jin M / O'Keefe, Christine / Brown-Countess, Arnyah / Peterson, Sara / Chen, Fan-En / Lenigk, Ralf / Trick, Alex / Wang, Tza-Huei / Puleo, Christopher

Antibiotics (Basel, Switzerland)

2023 Volume 12, Issue 11

Abstract: Antimicrobial resistance (AMR) is a global threat fueled by incorrect (and overuse) of antibiotic drugs, giving rise to the evolution of multi- and extreme drug-resistant bacterial strains. The longer time to antibiotic administration (TTA) associated ... ...

Abstract	Antimicrobial resistance (AMR) is a global threat fueled by incorrect (and overuse) of antibiotic drugs, giving rise to the evolution of multi- and extreme drug-resistant bacterial strains. The longer time to antibiotic administration (TTA) associated with the gold standard bacterial culture method has been responsible for the empirical usage of antibiotics and is a key factor in the rise of AMR. While polymerase chain reaction (PCR) and other nucleic acid amplification methods are rapidly replacing traditional culture methods, their scope has been restricted mainly to detect genotypic determinants of resistance and provide little to no information on phenotypic susceptibility to antibiotics. The work presented here aims to provide phenotypic antimicrobial susceptibility testing (AST) information by pairing short growth periods (~3-4 h) with downstream PCR assays to ultimately predict minimum inhibitory concentration (MIC) values of antibiotic treatment. To further simplify the dual workflows of the AST and PCR assays, these reactions are carried out in a single-vessel format (PCR tube) using novel lyophilized reagent beads (LRBs), which store dried PCR reagents along with primers and enzymes, and antibiotic drugs separately. The two reactions are separated in space and time using a melting paraffin wax seal, thus eliminating the need to transfer reagents across different consumables and minimizing user interactions. Finally, these two-step single-vessel reactions are multiplexed by using a microfluidic manifold that allows simultaneous testing of an unknown bacterial sample against different antibiotics at varying concentrations. The LRBs used in the microfluidic system showed no interference with the bacterial growth and PCR assays and provided an innovative platform for rapid point-of-care diagnostics (POC-Dx).
Language	English
Publishing date	2023-11-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2681345-2
ISSN	2079-6382
ISSN	2079-6382
DOI	10.3390/antibiotics12111641
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Article ; Online: Accelerating cutaneous healing in a rodent model of type II diabetes utilizing non-invasive focused ultrasound targeted at the spleen

Christine Morton / Victoria Cotero / Jeffrey Ashe / Fiona Ginty / Christopher Puleo

Frontiers in Neuroscience, Vol

2022 Volume 16

Abstract	Healing of wounds is delayed in Type 2 Diabetes Mellitus (T2DM), and new treatment approaches are urgently needed. Our earlier work showed that splenic pulsed focused ultrasound (pFUS) alters inflammatory cytokines in models of acute endotoxemia and pneumonia via modulation of the cholinergic anti-inflammatory pathway (CAP) (ref below). Based on these earlier results, we hypothesized that daily splenic exposure to pFUS during wound healing would accelerate closure rate via altered systemic cytokine titers. In this study, we applied non-invasive ultrasound directed to the spleen of a rodent model [Zucker Diabetic Sprague Dawley (ZDSD) rats] of T2DM with full thickness cutaneous excisional wounds in an attempt to accelerate wound healing via normalization of T2DM-driven aberrant cytokine expression. Daily (1x/day, Monday-Friday) pFUS pulses were targeted externally to the spleen area for 3 min over the course of 15 days. Wound diameter was measured daily, and levels of cytokines were evaluated in spleen and wound bed lysates. Non-invasive splenic pFUS accelerated wound closure by up to 4.5 days vs. sham controls. The time to heal in all treated groups was comparable to that of healthy rats from previously published studies (ref below), suggesting that the pFUS treatment restored a normal wound healing phenotype to the ZDSD rats. IL-6 was lower in stimulated spleen (-2.24 ± 0.81 Log2FC, p = 0.02) while L-selectin was higher in the wound bed of stimulated rodents (2.53 ± 0.72 Log2FC, p = 0.003). In summary, splenic pFUS accelerates healing in a T2DM rat model, demonstrating the potential of the method to provide a novel, non-invasive approach for wound care in diabetes.
Keywords	bioelectronic medicine ; nerve stimulation ; neuromodulation ; therapy ; ultrasound ; wound care ; Neurosciences. Biological psychiatry. Neuropsychiatry ; RC321-571
Subject code	630
Language	English
Publishing date	2022-11-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: A developmental atlas of somatosensory diversification and maturation in the dorsal root ganglia by single-cell mass cytometry.

Keeler, Austin B / Van Deusen, Amy L / Gadani, Irene C / Williams, Corey M / Goggin, Sarah M / Hirt, Ashley K / Vradenburgh, Shayla A / Fread, Kristen I / Puleo, Emily A / Jin, Lucy / Calhan, O Yipkin / Deppmann, Christopher D / Zunder, Eli R

Nature neuroscience

2022 Volume 25, Issue 11, Page(s) 1543–1558

Abstract: Precisely controlled development of the somatosensory system is essential for detecting pain, itch, temperature, mechanical touch and body position. To investigate the protein-level changes that occur during somatosensory development, we performed single- ...

Abstract	Precisely controlled development of the somatosensory system is essential for detecting pain, itch, temperature, mechanical touch and body position. To investigate the protein-level changes that occur during somatosensory development, we performed single-cell mass cytometry on dorsal root ganglia from C57/BL6 mice of both sexes, with litter replicates collected daily from embryonic day 11.5 to postnatal day 4. Measuring nearly 3 million cells, we quantified 30 molecularly distinct somatosensory glial and 41 distinct neuronal states across all timepoints. Analysis of differentiation trajectories revealed rare cells that co-express two or more Trk receptors and over-express stem cell markers, suggesting that these neurotrophic factor receptors play a role in cell fate specification. Comparison to previous RNA-based studies identified substantial differences between many protein-mRNA pairs, demonstrating the importance of protein-level measurements to identify functional cell states. Overall, this study demonstrates that mass cytometry is a high-throughput, scalable platform to rapidly phenotype somatosensory tissues.
MeSH term(s)	Male ; Female ; Mice ; Animals ; Ganglia, Spinal/physiology ; Neurons/physiology ; Neuroglia ; Cell Differentiation ; RNA, Messenger/genetics
Chemical Substances	RNA, Messenger
Language	English
Publishing date	2022-10-27
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	1420596-8
ISSN	1546-1726 ; 1097-6256
ISSN (online)	1546-1726
ISSN	1097-6256
DOI	10.1038/s41593-022-01181-8
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Zs.A 4891: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Targeted peripheral focused ultrasound stimulation attenuates obesity-induced metabolic and inflammatory dysfunctions.

Huerta, Tomás S / Devarajan, Alex / Tsaava, Tea / Rishi, Arvind / Cotero, Victoria / Puleo, Christopher / Ashe, Jeffrey / Coleman, Thomas R / Chang, Eric H / Tracey, Kevin J / Chavan, Sangeeta S

Scientific reports

2021 Volume 11, Issue 1, Page(s) 5083

Abstract: Obesity, a growing health concern, is associated with an increased risk of morbidity and mortality. Chronic low-grade inflammation is implicated in obesity-driven metabolic complications. Peripheral focused ultrasound stimulation (pFUS) is an emerging ... ...

Abstract	Obesity, a growing health concern, is associated with an increased risk of morbidity and mortality. Chronic low-grade inflammation is implicated in obesity-driven metabolic complications. Peripheral focused ultrasound stimulation (pFUS) is an emerging non-invasive technology that modulates inflammation. Here, we reasoned that focused ultrasound stimulation of the liver may alleviate obesity-related inflammation and other comorbidities. After 8 weeks on a high-fat high-carbohydrate "Western" diet, C57BL/6J mice were subjected to either sham stimulation or focused ultrasound stimulation at the porta hepatis. Daily liver-focused ultrasound stimulation for 8 weeks significantly decreased body weight, circulating lipids and mitigated dysregulation of adipokines. In addition, liver-focused ultrasound stimulation significantly reduced hepatic cytokine levels and leukocyte infiltration. Our findings demonstrate the efficacy of hepatic focused ultrasound for alleviating obesity and obesity-associated complications in mice. These findings suggest a previously unrecognized potential of hepatic focused ultrasound as a possible novel noninvasive approach in the context of obesity.
MeSH term(s)	Adipokines/blood ; Adipose Tissue/metabolism ; Adipose Tissue/radiation effects ; Adiposity/radiation effects ; Animals ; Cytokines/blood ; Diet, High-Fat/adverse effects ; Diet, Western/adverse effects ; Inflammation/metabolism ; Inflammation/therapy ; Lipid Metabolism/radiation effects ; Liver/metabolism ; Liver/radiation effects ; Male ; Mice ; Mice, Inbred C57BL ; Obesity/blood ; Obesity/etiology ; Obesity/therapy ; Signal Transduction/radiation effects ; Ultrasonic Therapy/methods ; Weight Gain/radiation effects
Chemical Substances	Adipokines ; Cytokines
Language	English
Publishing date	2021-03-03
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-84330-6
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Article ; Online: Robotic versus standard laparoscopic elective colectomy: where are the benefits?

Kulaylat, Audrey S / Mirkin, Katelin A / Puleo, Frances J / Hollenbeak, Christopher S / Messaris, Evangelos

The Journal of surgical research

2017 Volume 224, Page(s) 72–78

Abstract: Background: Robotic approaches for colorectal surgery have been growing in popularity as experience with the new technology develops, but are frequently associated with longer operative time. It is unclear whether prolonged operative duration in robotic ...

Abstract	Background: Robotic approaches for colorectal surgery have been growing in popularity as experience with the new technology develops, but are frequently associated with longer operative time. It is unclear whether prolonged operative duration in robotic cases translates to increased morbidity. This study aims to compare the outcomes of non-emergent laparoscopic and robotic colon resections. Methods: Patients undergoing non-emergent laparoscopic (LC) or robotic (RC) colon resections were identified in National Surgical Quality Improvement Project (2013-2015). Patients were matched 1:1 between cohorts using propensity score matching. To account for the prolonged operative time associated with robotic cases, operative times were stratified into approach-specific (LC or RC) tertiles (low, medium, and high) as covariates in the matching algorithm. Results: RC increased significantly over time and had lower conversion rates (6.0% among RC versus 11.5% among LC, P < 0.001). RC cases were longer (226 min versus 178 min, P < 0.001). Unadjusted complication rates were higher in the LC cohort (17.5% versus 15.2%, P < 0.001). After propensity score matching, RC was not associated with a significant difference in postoperative morbidity (15.2% among RC versus 15.9% among LC, P = 0.434). The robotic approach was associated with a one-half day shorter length of stay (4.6 d versus 5.2 d, P < 0.001), but similar 30-day readmission rates (8.9% versus 8.3%, P = 0.368). Conclusions: After controlling for operative duration and patient covariates, RC was associated with similar rates of postoperative morbidity, but decreased conversion rates and shorter length of stay. Further studies examining costs are needed to evaluate whether these benefits offset the increased costs associated with robotic approaches.
MeSH term(s)	Adult ; Aged ; Colectomy/adverse effects ; Colectomy/methods ; Elective Surgical Procedures/adverse effects ; Elective Surgical Procedures/methods ; Female ; Humans ; Laparoscopy/adverse effects ; Laparoscopy/methods ; Length of Stay ; Male ; Middle Aged ; Operative Time ; Robotic Surgical Procedures/adverse effects ; Robotic Surgical Procedures/methods
Language	English
Publishing date	2017-12-22
Publishing country	United States
Document type	Comparative Study ; Journal Article
ZDB-ID	80170-7
ISSN	1095-8673 ; 0022-4804
ISSN (online)	1095-8673
ISSN	0022-4804
DOI	10.1016/j.jss.2017.11.059
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Article ; Online: What is the significance of the in transit or interval sentinel node in melanoma?

Zager, Jonathan S / Puleo, Christopher A / Sondak, Vernon K

Annals of surgical oncology

2011 Volume 18, Issue 12, Page(s) 3232–3234

MeSH term(s)	Female ; Humans ; Lymph Nodes/pathology ; Lymph Nodes/surgery ; Lymphoscintigraphy ; Male ; Melanoma/pathology ; Melanoma/surgery ; Skin Neoplasms/pathology ; Skin Neoplasms/surgery
Language	English
Publishing date	2011-11
Publishing country	United States
Document type	Comment ; Editorial
ZDB-ID	1200469-8
ISSN	1534-4681 ; 1068-9265
ISSN (online)	1534-4681
ISSN	1068-9265
DOI	10.1245/s10434-011-1996-5
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Article: Droplet-Based Single-Cell Measurements of 16S rRNA Enable Integrated Bacteria Identification and Pheno-Molecular Antimicrobial Susceptibility Testing from Clinical Samples in 30 min.

Kaushik, Aniruddha M / Hsieh, Kuangwen / Mach, Kathleen E / Lewis, Shawna / Puleo, Christopher M / Carroll, Karen C / Liao, Joseph C / Wang, Tza-Huei

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

2021 Volume 8, Issue 6, Page(s) 2003419

Abstract: Empiric broad-spectrum antimicrobial treatments of urinary tract infections (UTIs) have contributed to widespread antimicrobial resistance. Clinical adoption of evidence-based treatments necessitates rapid diagnostic methods for pathogen identification ( ... ...

Abstract	Empiric broad-spectrum antimicrobial treatments of urinary tract infections (UTIs) have contributed to widespread antimicrobial resistance. Clinical adoption of evidence-based treatments necessitates rapid diagnostic methods for pathogen identification (ID) and antimicrobial susceptibility testing (AST) with minimal sample preparation. In response, a microfluidic droplet-based platform is developed for achieving both ID and AST from urine samples within 30 min. In this platform, fluorogenic hybridization probes are utilized to detect 16S rRNA from single bacterial cells encapsulated in picoliter droplets, enabling molecular identification of uropathogenic bacteria directly from urine in as little as 16 min. Moreover, in-droplet single-bacterial measurements of 16S rRNA provide a surrogate for AST, shortening the exposure time to 10 min for gentamicin and ciprofloxacin. A fully integrated device and screening workflow were developed to test urine specimens for one of seven unique diagnostic outcomes including the presence/absence of Gram-negative bacteria, molecular ID of the bacteriaas
Language	English
Publishing date	2021-02-01
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2808093-2
ISSN	2198-3844
ISSN	2198-3844
DOI	10.1002/advs.202003419
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