Result 1 - 10 of total 12
Journal of Patan Academy of Health Sciences
| Keywords | covid19 |
|---|---|
| Publisher | WHO |
| Document type | Article |
| Note | WHO #Covidence: #925894 |
| Database | COVID19 |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
bioRxiv
Abstract: Various reports of decreased analytical sensitivities of real-time PCR-based detection of Coronavirus Disease 2019 (COVID-19) have been associated with occurrence of mutations in the target area of primer/probe binding. Knowledge about propensities of ... ...
| Abstract | Various reports of decreased analytical sensitivities of real-time PCR-based detection of Coronavirus Disease 2019 (COVID-19) have been associated with occurrence of mutations in the target area of primer/probe binding. Knowledge about propensities of different genes to undergo mutation can inform researchers to select optimal genes to target for the qPCR design. We analyzed supplementary data from over 45 thousand SARS-CoV-2 genomes provided by Mercatelli et al to calculate the unique and prevalent mutations in different genes of SARS-CoV-2. We found that non-structural proteins in the ORF1ab region were more conserved compared to structural genes. Further factors which need to be relied upon for proper selection of genes for qPCR design are discussed. |
|---|---|
| Keywords | covid19 |
| Language | English |
| Publishing date | 2021-04-05 |
| Publisher | Cold Spring Harbor Laboratory |
| Document type | Article ; Online |
| DOI | 10.1101/2021.04.04.438420 |
| Database | COVID19 |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
Journal of Patan Academy of Health Sciences; Vol 7 No; 4-6 ; 2091-2757 ; 2091-2749
2020 Volume 1
| Keywords | covid19 |
|---|---|
| Language | English |
| Publishing date | 2020-04-30 |
| Publisher | Patan Academy of Health Sciences |
| Publishing country | np |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
Biosafety and health
2020 Volume 2, Issue 2, Page(s) 53–56
Abstract: The infrastructure needed to detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection (COVID-19) that complies completely with WHO guidelines is lacking across many parts of the globe, especially in developing countries, including ... ...
| Abstract | The infrastructure needed to detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection (COVID-19) that complies completely with WHO guidelines is lacking across many parts of the globe, especially in developing countries, including Nepal. We outline the problems faced by such countries and suggest that the national and international community should collaborate in the development and adoption of novel protocols for the rapid detection of COVID-19 according to locally available infrastructure, in order to fight against the outbreak. |
|---|---|
| Language | English |
| Publishing date | 2020-05-13 |
| Publishing country | Netherlands |
| Document type | Editorial |
| ISSN | 2590-0536 |
| ISSN (online) | 2590-0536 |
| DOI | 10.1016/j.bsheal.2020.05.002 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
2022 Volume 2022, Page(s) 2965666
Abstract: The genome sequence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been evolving via genomic drifts resulting in "emerging/drifting variants" circulating worldwide. The construction of polymerase chain reaction (PCR) assays for the ... ...
| Abstract | The genome sequence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been evolving via genomic drifts resulting in "emerging/drifting variants" circulating worldwide. The construction of polymerase chain reaction (PCR) assays for the reliable, efficient, and specific diagnosis of the drifting variants of SARS-CoV-2 is specifically governed by the selection and construction of primers and probes. The efficiency of molecular diagnosis is impacted by the identity/homology of the genome sequence of SARS-CoV-2 with other coronaviruses, drifting variants or variants of concern (VOCs) circulating in communities, inherent capacity of mutation(s) of various target genes of SARS-CoV-2, and concentration of genes of interest in host cells. The precise amplicon selection and construction of primers and probes for PCR-based assays can efficiently discriminate specific SARS-CoV-2 drifting variants. The construction of single nucleotide polymorphism (SNP)-specific primers and probes for PCR assays is pivotal to specifically distinguish SARS-CoV-2 variants present in the communities and contributes to better diagnosis and prevention of the ongoing COVID-19 pandemic. In this study, we have utilized |
|---|---|
| Language | English |
| Publishing date | 2022-05-13 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 2625776-2 |
| ISSN | 1687-8647 ; 1687-8639 |
| ISSN (online) | 1687-8647 |
| ISSN | 1687-8639 |
| DOI | 10.1155/2022/2965666 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
2020 Volume 53, Page(s) 101599
Abstract: Most of the COVID-19 cases in Nepal are in the Southern districts of Nepal bordering India with travel histories to India.•Very few positive cases of COVID-19 are detected in Nepal which could either be due to early national lockdown.•Low PCR positivity ...
| Abstract | •Most of the COVID-19 cases in Nepal are in the Southern districts of Nepal bordering India with travel histories to India.•Very few positive cases of COVID-19 are detected in Nepal which could either be due to early national lockdown.•Low PCR positivity rates could also be due to inefficiency of the PCR methods.•Whole genomes of 93 clinical samples from COVID-19 patients were analyzed to find the primer and probe binding sites.•Mutations in probe binding sites were found which could impact PCR efficiency resulting in false negative results. |
|---|---|
| MeSH term(s) | Betacoronavirus/classification ; Betacoronavirus/genetics ; Betacoronavirus/pathogenicity ; COVID-19 ; COVID-19 Testing ; Clinical Laboratory Techniques/methods ; Coronavirus Infections/diagnosis ; Coronavirus Infections/epidemiology ; Coronavirus Infections/transmission ; Coronavirus Infections/virology ; DNA Probes/standards ; False Negative Reactions ; Genome, Viral ; Humans ; India/epidemiology ; Mutation ; Nepal/epidemiology ; Pandemics ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/epidemiology ; Pneumonia, Viral/transmission ; Pneumonia, Viral/virology ; RNA, Viral/genetics ; Reagent Kits, Diagnostic/standards ; Reverse Transcriptase Polymerase Chain Reaction/standards ; SARS-CoV-2 ; Severity of Illness Index ; Transients and Migrants |
| Chemical Substances | DNA Probes ; RNA, Viral ; Reagent Kits, Diagnostic |
| Keywords | covid19 |
| Language | English |
| Publishing date | 2020-05-18 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 639082-1 |
| ISSN | 1096-1194 ; 0890-8508 |
| ISSN (online) | 1096-1194 |
| ISSN | 0890-8508 |
| DOI | 10.1016/j.mcp.2020.101599 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Zs.A 2230: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
Biosafety and Health
Nepal as a case study
2020 Volume 2, Issue 2, Page(s) 53–56
| Keywords | covid19 |
|---|---|
| Language | English |
| Publisher | Elsevier BV |
| Publishing country | us |
| Document type | Article ; Online |
| ISSN | 2590-0536 |
| DOI | 10.1016/j.bsheal.2020.05.002 |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
2020 Volume 53, Page(s) 101599
| Keywords | Cell Biology ; Molecular Biology ; covid19 |
|---|---|
| Language | English |
| Publisher | Elsevier BV |
| Publishing country | us |
| Document type | Article ; Online |
| ZDB-ID | 639082-1 |
| ISSN | 1096-1194 ; 0890-8508 |
| ISSN (online) | 1096-1194 |
| ISSN | 0890-8508 |
| DOI | 10.1016/j.mcp.2020.101599 |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
| Zs.A 2230: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
Biosafety and Health, Vol 2, Iss 2, Pp 53-
Nepal as a case study
2020 Volume 56
Abstract: The infrastructure needed to detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection (COVID-19) that complies completely with WHO guidelines is lacking across many parts of the globe, especially in developing countries, including ... ...
| Abstract | The infrastructure needed to detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection (COVID-19) that complies completely with WHO guidelines is lacking across many parts of the globe, especially in developing countries, including Nepal. We outline the problems faced by such countries and suggest that the national and international community should collaborate in the development and adoption of novel protocols for the rapid detection of COVID-19 according to locally available infrastructure, in order to fight against the outbreak. |
|---|---|
| Keywords | COVID-19 ; SARS-CoV-2 ; Loop-mediated isothermal amplification (LAMP) assay ; Biosafety Level-2 (BSL-2) ; Infectious and parasitic diseases ; RC109-216 ; Public aspects of medicine ; RA1-1270 ; covid19 |
| Language | English |
| Publishing date | 2020-06-01T00:00:00Z |
| Publisher | Elsevier |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
medRxiv
Abstract: The novel corona virus disease -2019 (COVID-19) pandemic has caused a massive global public health havoc. Recent clinical trials carried out in China has found a promising therapeutic application of chloroquine and hydroxychloroquine for COVID-19. This ... ...
| Abstract | The novel corona virus disease -2019 (COVID-19) pandemic has caused a massive global public health havoc. Recent clinical trials carried out in China has found a promising therapeutic application of chloroquine and hydroxychloroquine for COVID-19. This study meticulously evaluated the various dosages of chloroquine and hydroxychloroquine utilized in clinical trials registered in Chinese and US clinical trial registries for the treatment of pneumonia caused by SARS-CoV-2. |
|---|---|
| Keywords | covid19 |
| Language | English |
| Publishing date | 2020-03-27 |
| Publisher | Cold Spring Harbor Laboratory Press |
| Document type | Article ; Online |
| DOI | 10.1101/2020.03.22.20040964 |
| Database | COVID19 |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.