LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 61

Search options

  1. Book ; Thesis: Regulation of glucagon secretion from pancreatic alpha cells

    De Marinis, Yang Zhang

    (Doctoral dissertation series ; 2010,4)

    2010  

    Author's details Yang Zhang De Marinis
    Series title Doctoral dissertation series ; 2010,4
    Collection
    Language English
    Size Getr. Zählung : Ill., graph. Darst.
    Publishing country Sweden
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Lund, Univ., Diss., 2010
    Note Zsfassung in schwed. Sprache ; Enth. 3 Sonderabdr.
    HBZ-ID HT016830769
    ISBN 978-91-86443-18-4 ; 91-86443-18-6
    Database Catalogue ZB MED Medicine, Health

    Kategorien

  2. Article ; Online: Elevated circulating follistatin associates with increased risk of mortality and cardiometabolic disorders.

    Pan, Jingxue / Nilsson, Jan / Engström, Gunnar / De Marinis, Yang

    Nutrition, metabolism, and cardiovascular diseases : NMCD

    2023  Volume 34, Issue 2, Page(s) 418–425

    Abstract: Background and aims: Previous study showed that elevated circulating hepatokine follistatin (FST) associates with an increased risk of type 2 diabetes by inducing adipose tissue insulin resistance. Here we explore further the relationships between ... ...

    Abstract Background and aims: Previous study showed that elevated circulating hepatokine follistatin (FST) associates with an increased risk of type 2 diabetes by inducing adipose tissue insulin resistance. Here we explore further the relationships between plasma FST levels with mortality and health outcomes.
    Methods and results: The population-based Malmö Diet Cancer cardiovascular cohort (n = 4733, age 45-68 years) was used to study plasma FST in relation to incidence of health outcomes, by linkage with national patient registers. Cox regression analysis was used to assess the associations of plasma FST and outcomes, with adjustments for multiple potential confounding factors. During the mean follow-up time of 22.64 ± 5.84 years in 4,733 individuals, 526 had incident stroke, 432 had ischemic stroke, 530 had incident coronary events (CE), 339 had incident heart failure (HF), 320 had incident chronic kidney disease (CKD) and 1,843 individuals died. Hazard ratio (HR) per standard deviation increase in FST levels adjusted for multiple risk factors was 1.05 (95%CI: 1.00-1.11, p = 0.036) for mortality; 1.10 (95%CI: 1.00-1.20, p = 0.042) for stroke; 1.13 (95%CI: 1.03-1.25, p = 0.014) for ischemic stroke; 1.16 (95%CI: 1.03-1.30, p = 0.015) for HF; and 1.38 (95%CI: 1.12-1.70, p = 0.003) for a diagnosis of CKD. In MDC-CC individuals without prevalent or incident diabetes, the association between FST and stroke, CE and CKD remained significant; but not with mortality or HF.
    Conclusions: Elevated circulating FST associates with an increased risk of mortality and HF, which partly may be mediated by diabetes. FST also associated with stroke, ischemic stroke, CE and CKD, independently of established risk factors including diabetes.
    MeSH term(s) Aged ; Humans ; Middle Aged ; Diabetes Mellitus, Type 2/diagnosis ; Follistatin ; Heart Failure ; Ischemic Stroke ; Renal Insufficiency, Chronic ; Stroke/diagnosis
    Chemical Substances Follistatin ; FST protein, human
    Language English
    Publishing date 2023-09-19
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1067704-5
    ISSN 1590-3729 ; 0939-4753
    ISSN (online) 1590-3729
    ISSN 0939-4753
    DOI 10.1016/j.numecd.2023.09.012
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  3. Article ; Online: Deep learning to automatically classify very large sets of preoperative and postoperative shoulder arthroplasty radiographs.

    Yang, Linjun / Oeding, Jacob F / de Marinis, Rodrigo / Marigi, Erick / Sanchez-Sotelo, Joaquin

    Journal of shoulder and elbow surgery

    2023  Volume 33, Issue 4, Page(s) 773–780

    Abstract: Background: Joint arthroplasty registries usually lack information on medical imaging owing to the laborious process of observing and recording, as well as the lack of standard methods to transfer the imaging information to the registries, which can ... ...

    Abstract Background: Joint arthroplasty registries usually lack information on medical imaging owing to the laborious process of observing and recording, as well as the lack of standard methods to transfer the imaging information to the registries, which can limit the investigation of various research questions. Artificial intelligence (AI) algorithms can automate imaging-feature identification with high accuracy and efficiency. With the purpose of enriching shoulder arthroplasty registries with organized imaging information, it was hypothesized that an automated AI algorithm could be developed to classify and organize preoperative and postoperative radiographs from shoulder arthroplasty patients according to laterality, radiographic projection, and implant type.
    Methods: This study used a cohort of 2303 shoulder radiographs from 1724 shoulder arthroplasty patients. Two observers manually labeled all radiographs according to (1) laterality (left or right), (2) projection (anteroposterior, axillary, or lateral), and (3) whether the radiograph was a preoperative radiograph or showed an anatomic total shoulder arthroplasty or a reverse shoulder arthroplasty. All these labeled radiographs were randomly split into developmental and testing sets at the patient level and based on stratification. By use of 10-fold cross-validation, a 3-task deep-learning algorithm was trained on the developmental set to classify the 3 aforementioned characteristics. The trained algorithm was then evaluated on the testing set using quantitative metrics and visual evaluation techniques.
    Results: The trained algorithm perfectly classified laterality (F1 scores [harmonic mean values of precision and sensitivity] of 100% on the testing set). When classifying the imaging projection, the algorithm achieved F1 scores of 99.2%, 100%, and 100% on anteroposterior, axillary, and lateral views, respectively. When classifying the implant type, the model achieved F1 scores of 100%, 95.2%, and 100% on preoperative radiographs, anatomic total shoulder arthroplasty radiographs, and reverse shoulder arthroplasty radiographs, respectively. Visual evaluation using integrated maps showed that the algorithm focused on the relevant patient body and prosthesis parts for classification. It took the algorithm 20.3 seconds to analyze 502 images.
    Conclusions: We developed an efficient, accurate, and reliable AI algorithm to automatically identify key imaging features of laterality, imaging view, and implant type in shoulder radiographs. This algorithm represents the first step to automatically classify and organize shoulder radiographs on a large scale in very little time, which will profoundly enrich shoulder arthroplasty registries.
    MeSH term(s) Humans ; Arthroplasty, Replacement, Shoulder ; Shoulder Joint/diagnostic imaging ; Shoulder Joint/surgery ; Deep Learning ; Artificial Intelligence ; Radiography ; Retrospective Studies
    Language English
    Publishing date 2023-10-23
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1170782-3
    ISSN 1532-6500 ; 1058-2746
    ISSN (online) 1532-6500
    ISSN 1058-2746
    DOI 10.1016/j.jse.2023.09.021
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  4. Article ; Online: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line.

    Aldén, Markus / Olofsson Falla, Francisko / Yang, Daowei / Barghouth, Mohammad / Luan, Cheng / Rasmussen, Magnus / De Marinis, Yang

    Current issues in molecular biology

    2022  Volume 44, Issue 3, Page(s) 1115–1126

    Abstract: Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the BNT162b2 injection. Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse-transcribed ... ...

    Abstract Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the BNT162b2 injection. Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse-transcribed and integrated into the genome of human cells. In this study, we investigated the effect of BNT162b2 on the human liver cell line Huh7 in vitro. Huh7 cells were exposed to BNT162b2, and quantitative PCR was performed on RNA extracted from the cells. We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. PCR on genomic DNA of Huh7 cells exposed to BNT162b2 amplified the DNA sequence unique to BNT162b2. Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. We also show that BNT162b2 mRNA is reverse transcribed intracellularly into DNA in as fast as 6 h upon BNT162b2 exposure.
    Language English
    Publishing date 2022-02-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2000024-8
    ISSN 1467-3045 ; 1467-3037
    ISSN (online) 1467-3045
    ISSN 1467-3037
    DOI 10.3390/cimb44030073
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  5. Article ; Online: Post-outbreak serological screening for SARS-CoV-2 infection in healthcare workers at a Swedish University Hospital.

    Strand, Rasmus / Fernström, Nils / Holmberg, Anna / De Marinis, Yang / Fraenkel, Carl-Johan / Rasmussen, Magnus

    Infectious diseases (London, England)

    2021  Volume 53, Issue 9, Page(s) 707–712

    Abstract: Background: Nosocomial outbreaks of coronavirus disease 2019 (COVID-19) can have devastating consequences from both a resource cost and patient healthcare perspective. Relying on reverse transcription-polymerase chain reaction (RT-PCR) for identifying ... ...

    Abstract Background: Nosocomial outbreaks of coronavirus disease 2019 (COVID-19) can have devastating consequences from both a resource cost and patient healthcare perspective. Relying on reverse transcription-polymerase chain reaction (RT-PCR) for identifying infected individuals may result in missed cases. Screening for antibodies after an outbreak can help to find missed cases and better illuminate routes of transmission.
    Methods: In this study, we present the results of a serological screening of the healthcare workers (HCWs) on a ward for infectious diseases in Sweden with a point-of-care antibody test 8 weeks after an outbreak of COVID-19. In all, 107/123 (87%) of HCWs who were tested with RT-PCR in the outbreak investigation participated in this study on seroprevalence. Participants were also asked to fill out a questionnaire entailing epidemiological data. The cohort was stratified by RT-PCR result and the resulting groups were compared to each other.
    Results: Six (8%) HCWs who were tested RT-PCR negative during the outbreak investigation had developed specific IgG antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). These HCWs had all worked shifts with colleagues who later were tested RT-PCR positive during the outbreak.
    Conclusions: Our results indicate that a serological follow-up screening after an outbreak may be used as a complement to virus detection in an outbreak situation. However, immunoglobulin (Ig) G-detection should also be performed at the start of an outbreak, to facilitate interpretation of the results.
    MeSH term(s) Antibodies, Viral ; COVID-19 ; Disease Outbreaks ; Health Personnel ; Humans ; SARS-CoV-2 ; Seroepidemiologic Studies ; Sweden/epidemiology
    Chemical Substances Antibodies, Viral
    Language English
    Publishing date 2021-05-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 2839775-7
    ISSN 2374-4243 ; 2374-4235
    ISSN (online) 2374-4243
    ISSN 2374-4235
    DOI 10.1080/23744235.2021.1925739
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  6. Article ; Online: Current clinical applications of artificial intelligence in shoulder surgery: what the busy shoulder surgeon needs to know and what's coming next.

    de Marinis, Rodrigo / Marigi, Erick M / Atwan, Yousif / Yang, Linjun / Oeding, Jacob F / Gupta, Puneet / Pareek, Ayoosh / Sanchez-Sotelo, Joaquin / Sperling, John W

    JSES reviews, reports, and techniques

    2023  Volume 3, Issue 4, Page(s) 447–453

    Abstract: Background: Artificial intelligence (AI) is a continuously expanding field with the potential to transform a variety of industries-including health care-by providing automation, efficiency, precision, accuracy, and decision-making support for simple and ...

    Abstract Background: Artificial intelligence (AI) is a continuously expanding field with the potential to transform a variety of industries-including health care-by providing automation, efficiency, precision, accuracy, and decision-making support for simple and complex tasks. Basic knowledge of the key features as well as limitations of AI is paramount to understand current developments in this field and to successfully apply them to shoulder surgery. The purpose of the present review is to provide an overview of AI within orthopedics and shoulder surgery exploring current and forthcoming AI applications.
    Methods: PubMed and Scopus databases were searched to provide a narrative review of the most relevant literature on AI applications in shoulder surgery.
    Results: Despite the enormous clinical and research potential of AI, orthopedic surgery has been a relatively late adopter of AI technologies. Image evaluation, surgical planning, aiding decision-making, and facilitating patient evaluations over time are some of the current areas of development with enormous opportunities to improve surgical practice, research, and education. Furthermore, the advancement of AI-driven strategies has the potential to create a more efficient medical system that may reduce the overall cost of delivering and implementing quality health care for patients with shoulder pathology.
    Conclusion: AI is an expanding field with the potential for broad clinical and research applications in orthopedic surgery. Many challenges still need to be addressed to fully leverage the potential of AI to clinical practice and research such as privacy issues, data ownership, and external validation of the proposed models.
    Language English
    Publishing date 2023-09-07
    Publishing country Netherlands
    Document type Journal Article ; Review
    ISSN 2666-6391
    ISSN (online) 2666-6391
    DOI 10.1016/j.xrrt.2023.07.008
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  7. Article ; Online: Association between SARS-CoV-2 and exposure risks in health care workers and university employees - a cross-sectional study.

    Nygren, David / Norén, Jonas / De Marinis, Yang / Holmberg, Anna / Fraenkel, Carl-Johan / Rasmussen, Magnus

    Infectious diseases (London, England)

    2021  Volume 53, Issue 6, Page(s) 460–468

    Abstract: Background: In health care workers SARS-CoV-2 has been shown to be an occupational health risk, often associated with transmission between health care workers. Yet, insufficient information on transmission dynamics has been presented to elucidate the ... ...

    Abstract Background: In health care workers SARS-CoV-2 has been shown to be an occupational health risk, often associated with transmission between health care workers. Yet, insufficient information on transmission dynamics has been presented to elucidate the precise risk factors for contracting SARS-CoV-2 in this group.
    Methods: In this cross-sectional study, we investigated association between questionnaire answers on potential exposure situations and SARS-CoV-2-positivity. Health care workers with and without COVID-19-patient contact at nine units at Skåne University Hospitals in Malmö and Lund, Sweden and university employees from Lund University, Sweden were enrolled. To limit impact of health care worker to health care worker transmission, units with known outbreaks were excluded. A SARS-CoV-2-positive case was defined by a previous positive PCR or anti-SARS-CoV-2 IgG in the
    Results: SARS-CoV-2-positivity was detected in 11/51 (22%) health care workers in COVID-19-units, 10/220 (5%) in non-COVID-19-units and 11/192 (6%) University employees (
    Conclusion: Our study confirmed previous findings of elevated risk of acquiring SARS-CoV-2 in health care workers in COVID-19-units, despite exclusion of units with known outbreaks. Interestingly, health care workers in non-COVID-19-units had similar risk as University employees. Further measures to improve the safety of health care workers might be needed.KEY POINTSPrevious findings of elevated risk of contracting SARS-CoV-2 in health care workers with COVID-19 patient contact was confirmed, despite exclusion of wards with known SARS-CoV-2 outbreaks. Further measures to improve the safety of health care workers might be needed.
    MeSH term(s) COVID-19 ; Cross-Sectional Studies ; Health Personnel ; Humans ; SARS-CoV-2 ; Sweden
    Language English
    Publishing date 2021-03-09
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2839775-7
    ISSN 2374-4243 ; 2374-4235
    ISSN (online) 2374-4243
    ISSN 2374-4235
    DOI 10.1080/23744235.2021.1892819
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  8. Article: Detection of SARS-CoV-2 by rapid antigen tests on saliva in hospitalized patients with COVID-19.

    De Marinis, Yang / Pesola, Anne-Katrine / Söderlund Strand, Anna / Norman, Astrid / Pernow, Gustav / Aldén, Markus / Yang, Runtao / Rasmussen, Magnus

    Infection ecology & epidemiology

    2021  Volume 11, Issue 1, Page(s) 1993535

    Abstract: Background: The COVID-19 pandemic presents great challenges on transmission prevention, and rapid diagnosis is essential to reduce the disease spread. Various diagnostic methods are available to identify an ongoing infection by nasopharyngeal (NPH) swab ...

    Abstract Background: The COVID-19 pandemic presents great challenges on transmission prevention, and rapid diagnosis is essential to reduce the disease spread. Various diagnostic methods are available to identify an ongoing infection by nasopharyngeal (NPH) swab sampling. However, the procedure requires handling by health care professionals, and therefore limits the application in household and community settings.
    Objectives: In this study, we aimed to determine if the detection of SARS-CoV-2 can be performed alternatively on saliva specimens by rapid antigen test.
    Study design: Saliva and NPH specimens were collected from 44 patients with confirmed COVID-19. To assess the diagnostic accuracy of point-of-care SARS-CoV-2 rapid antigen test on saliva specimens, we compared the performance of four test products.
    Results: RT-qPCR was performed and NPH and saliva sampling had similar Ct values, which associated with disease duration. All four antigen tests showed similar trend in detecting SARS-CoV-2 in saliva, but with variation in the ability to detect positive cases. The rapid antigen test with the best performance could detect up to 67% of the positive cases with Ct values lower than 25, and disease duration shorter than 10 days.
    Conclusion: Our study therefore supports saliva testing as an alternative diagnostic procedure to NPH testing, and that rapid antigen test on saliva provides a potential complement to PCR test to meet increasing screening demand.
    Language English
    Publishing date 2021-10-29
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2627673-2
    ISSN 2000-8686
    ISSN 2000-8686
    DOI 10.1080/20008686.2021.1993535
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  9. Article ; Online: PTPD: predicting therapeutic peptides by deep learning and word2vec.

    Wu, Chuanyan / Gao, Rui / Zhang, Yusen / De Marinis, Yang

    BMC bioinformatics

    2019  Volume 20, Issue 1, Page(s) 456

    Abstract: Background In the search for therapeutic peptides for disease treatments, many efforts have been made to identify various functional peptides from large numbers of peptide sequence databases. In this paper, we propose an effective computational model ... ...

    Abstract *: Background In the search for therapeutic peptides for disease treatments, many efforts have been made to identify various functional peptides from large numbers of peptide sequence databases. In this paper, we propose an effective computational model that uses deep learning and word2vec to predict therapeutic peptides (PTPD). *: Results Representation vectors of all k-mers were obtained through word2vec based on k-mer co-existence information. The original peptide sequences were then divided into k-mers using the windowing method. The peptide sequences were mapped to the input layer by the embedding vector obtained by word2vec. Three types of filters in the convolutional layers, as well as dropout and max-pooling operations, were applied to construct feature maps. These feature maps were concatenated into a fully connected dense layer, and rectified linear units (ReLU) and dropout operations were included to avoid over-fitting of PTPD. The classification probabilities were generated by a sigmoid function. PTPD was then validated using two datasets: an independent anticancer peptide dataset and a virulent protein dataset, on which it achieved accuracies of 96% and 94%, respectively. *: Conclusions PTPD identified novel therapeutic peptides efficiently, and it is suitable for application as a useful tool in therapeutic peptide design.
    MeSH term(s) Computational Biology/methods ; Databases, Nucleic Acid ; Deep Learning ; Drug Discovery ; Peptides/therapeutic use
    Chemical Substances Peptides
    Language English
    Publishing date 2019-09-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 2041484-5
    ISSN 1471-2105 ; 1471-2105
    ISSN (online) 1471-2105
    ISSN 1471-2105
    DOI 10.1186/s12859-019-3006-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

  10. Article ; Online: FS-GBDT: identification multicancer-risk module via a feature selection algorithm by integrating Fisher score and GBDT.

    Zhang, Jialin / Xu, Da / Hao, Kaijing / Zhang, Yusen / Chen, Wei / Liu, Jiaguo / Gao, Rui / Wu, Chuanyan / De Marinis, Yang

    Briefings in bioinformatics

    2021  Volume 22, Issue 3

    Abstract: Cancer is a highly heterogeneous disease caused by dysregulation in different cell types and tissues. However, different cancers may share common mechanisms. It is critical to identify decisive genes involved in the development and progression of cancer, ...

    Abstract Cancer is a highly heterogeneous disease caused by dysregulation in different cell types and tissues. However, different cancers may share common mechanisms. It is critical to identify decisive genes involved in the development and progression of cancer, and joint analysis of multiple cancers may help to discover overlapping mechanisms among different cancers. In this study, we proposed a fusion feature selection framework attributed to ensemble method named Fisher score and Gradient Boosting Decision Tree (FS-GBDT) to select robust and decisive feature genes in high-dimensional gene expression datasets. Joint analysis of 11 human cancers types was conducted to explore the key feature genes subset of cancer. To verify the efficacy of FS-GBDT, we compared it with four other common feature selection algorithms by Support Vector Machine (SVM) classifier. The algorithm achieved highest indicators, outperforms other four methods. In addition, we performed gene ontology analysis and literature validation of the key gene subset, and this subset were classified into several functional modules. Functional modules can be used as markers of disease to replace single gene which is difficult to be found repeatedly in applications of gene chip, and to study the core mechanisms of cancer.
    MeSH term(s) Algorithms ; Cluster Analysis ; Computational Biology/methods ; Decision Trees ; Gene Expression Profiling/classification ; Gene Expression Profiling/methods ; Gene Expression Regulation, Neoplastic ; Gene Ontology ; Humans ; Neoplasms/genetics ; Neoplasms/pathology ; Reproducibility of Results ; Support Vector Machine
    Language English
    Publishing date 2021-05-19
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2068142-2
    ISSN 1477-4054 ; 1467-5463
    ISSN (online) 1477-4054
    ISSN 1467-5463
    DOI 10.1093/bib/bbaa189
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

To top