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Article ; Online: Comparison of Zinc Oxide Nanoparticle Integration into Non-Woven Fabrics Using Different Functionalisation Methods for Prospective Application as Active Facemasks.

Ferreira, Tânia / Vale, Ana Catarina / Pinto, Alexandra C / Costa, Rita V / Pais, Vânia / Sousa, Diana / Gomes, Fernanda / Pinto, Graça / Dias, José Guilherme / Moreira, Inês P / Mota, Carlos / Bessa, João / Antunes, Joana C / Henriques, Mariana / Cunha, Fernando / Fangueiro, Raul

Polymers

2023 Volume 15, Issue 17

Abstract: ... preparedness. In this work, different polypropylene non-woven fabrics (NWF) were characterised regarding ... their antimicrobial properties. The functionalised fabrics obtained via dip-pad-dry unveiled the most promising data, with 0.017 ...

Abstract	The development of advanced facemasks stands out as a paramount priority in enhancing healthcare preparedness. In this work, different polypropylene non-woven fabrics (NWF) were characterised regarding their structural, physicochemical and comfort-related properties. The selected NWF for the intermediate layer was functionalised with zinc oxide nanoparticles (ZnO NPs) 0.3 and 1.2wt% using three different methods: electrospinning, dip-pad-dry and exhaustion. After the confirmation of ZnO NP content and distribution within the textile fibres by morphological and chemical analysis, the samples were evaluated regarding their antimicrobial properties. The functionalised fabrics obtained via dip-pad-dry unveiled the most promising data, with 0.017 ± 0.013wt% ZnO NPs being mostly located at the fibre's surface and capable of total eradication of
Language	English
Publishing date	2023-08-22
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527146-5
ISSN	2073-4360 ; 2073-4360
ISSN (online)	2073-4360
ISSN	2073-4360
DOI	10.3390/polym15173499
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Article: Impact of fabrics from transgenic flax on cultures of skin cells.

Gąsiorowski, Kazimierz / Gębarowski, Tomasz / Moreira, Helena / Kulma, Anna / Szatkowski, Michał / Szopa, Jan

Advances in clinical and experimental medicine : official organ Wroclaw Medical University

2019 Volume 28, Issue 4, Page(s) 431–438

Abstract: ... The objective of this study was to compare the impact of flax fabrics from transgenic plants overexpressing ... phenolic acids and flavonoids (W92) and polyhydroxybutyrate (M48), as well as fabric from non-transgenic plant ... Nike) on cultures of human skin cells.: Material and methods: Flax fabric pieces as well as water ...

Abstract	Background: The development of a new type of wound dressing material that can support skin regeneration is an important challenge to improve treatment of chronic, non-healing wounds. Objectives: The objective of this study was to compare the impact of flax fabrics from transgenic plants overexpressing phenolic acids and flavonoids (W92) and polyhydroxybutyrate (M48), as well as fabric from non-transgenic plant (Nike) on cultures of human skin cells. Material and methods: Flax fabric pieces as well as water extracts from the fabrics were co-cultured with human skin cells: keratinocytes, fibroblasts, dermal microvascular endothelial cells, and with monocytoid cell line (THP1) for 48 h. Cell viability and proliferation were assessed with the sulforhodamine B colorimetric assay. Intracellular reactive oxygen species (ROS) was estimated with the 2'7 dichlorodihydrofluorescein diacetate (DCFH-DA) oxidation method. Endothelial cell migration was measured with the scratch assay. The results were compared with the multi-criteria analysis (MCA) procedure. Results: Tested flax fabrics released flavonoids and polyhydroxybutyrate to cell culture media, as it was determined by means of the high performance liquid chromatography (HPLC) method. Fabrics from transgenic plants W92 and M48 promoted proliferation of keratinocytes and fibroblasts. Water extracts from flax fabric diminished the proliferation of monocytoid cells, decreased oxidative burst in activated THP1 cells and accelerated the velocity of dermal microvascular cell migration. The MCA proved that the sum of beneficial effects estimated in human skin cell cultures was higher (by 47% and by 34% with W92 and M48, respectively) than that of non-transgenic flax fabric (Nike). Conclusions: The W92 and M48 fabrics should be further studied as candidates for elaboration of new types of bandages, able to improve skin wound healing.
MeSH term(s)	Biotechnology ; Cell Movement ; Fibroblasts/drug effects ; Fibroblasts/metabolism ; Flax/genetics ; Genetic Engineering/methods ; Humans ; Plant Preparations/pharmacology ; Plants, Genetically Modified ; Skin
Chemical Substances	Plant Preparations
Language	English
Publishing date	2019-01-17
Publishing country	Poland
Document type	Comparative Study ; Journal Article
ZDB-ID	2270257-X
ISSN	1899-5276 ; 1230-025X
ISSN	1899-5276 ; 1230-025X
DOI	10.17219/acem/92563
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Article ; Online: Traditional Fabric and Medicinal Use Are the Leading Factors of In Situ Conservation of Gossypium barbadense in Central Brazil

Kálita Cristina Moreira Cardoso / Guilherme Hoffmann Barroso / Fabio Oliveira Freitas / Ivandilson Pessoa Pinto de Menezes / Catarina Fernandes Silva / Nair Helena Castro Arriel / Valdinei Sofiatti / Lucia Vieira Hoffmann

Sustainability, Vol 15, Iss 4552, p

2023 Volume 4552

Abstract: ... of Gossypium barbadense . Medicinal use is more determinant than hand weaving in deciding to keep plants and seeds. Fabric ...

Abstract	The Sea Island cotton Gossypium barbadense has been present in Brazil for at least 750 years. Cultivated worldwide, the fibres present superior quality; therefore, farmers’ seeds are an important genetic resource and in situ maintenance is essential to complement ex situ conservation. To understand how the species has been conserved in situ and investigate the socio-economic aspects which may ensure the continuity of its conservation, we conducted expeditions to three different municipalities situated in Brazilian Cerrado, Goiás state, Brazil—one of which is a traditional community, the quilombo Kalunga community—interviewed plant maintainers and compared our results with data from the Brazilian Institute of Geography and Statistics. There is hand spinning and hand weaving for home uses and commercialization within and outside the traditional community, which contribute to the continuity of the in situ conservation of Gossypium barbadense . Medicinal use is more determinant than hand weaving in deciding to keep plants and seeds. Fabric handicraft is a predominantly female, low-income activity. Interviews with cotton hand spinners indicated that in situ maintenance may be favoured by access to weaving looms and improvement in the marketing and sale of their products. Policies valuing handicrafts can ensure the continuity of biodiversity and disseminate and vivify traditions in addition to maintaining an income for the artisans.
Keywords	genetic resources ; in situ maintenance ; medicinal plants ; Pima cotton ; quilombo ; Sea Island cotton ; Environmental effects of industries and plants ; TD194-195 ; Renewable energy sources ; TJ807-830 ; Environmental sciences ; GE1-350
Subject code	333
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Computer-aided technology for fabricating removable partial denture frameworks: A systematic review and meta-analysis.

Pordeus, Mariana Domingues / Santiago Junior, Joel Ferreira / Venante, Helena Sandrini / Bringel da Costa, Rodrigo Moreira / Chappuis Chocano, Ana Paula / Porto, Vinicius Carvalho

The Journal of prosthetic dentistry

2021 Volume 128, Issue 3, Page(s) 331–340

Abstract: ... search of studies published up to September 2019 was performed in PubMed/MEDLINE, Web of Science ...

Abstract	Statement of problem: A consensus that establishes the indications and clinical performance of removable partial denture (RPD) frameworks designed and manufactured with computer-aided design and computer-aided manufacturing (CAD-CAM) systems is lacking. Purpose: The purpose of this systematic review and meta-analysis was to evaluate the currently published literature investigating different CAD-CAM methods and techniques for RPD manufacturing and their clinical performance. Material and methods: A comprehensive search of studies published up to September 2019 was performed in PubMed/MEDLINE, Web of Science, Cochrane Library, and SciELO databases according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA Statement) criteria and was registered and approved in the International Prospective Register of Systematic Reviews (PROSPERO: CRD42020152197). The population, intervention, comparison, outcome (PICO) question was "Do the CAD-CAM frameworks have similar performances to those fabricated by conventional techniques?" The meta-analysis included clinical and in vitro studies based on the effect size and test of Null (2-Tail) with 95% confidence interval (CI). Clinical and in vitro studies were selected and analyzed separately. Results: A total of 15 articles out of 358 were selected. For clinical studies, quantitative analysis with a sample of 25 participants showed a mean discrepancy between occlusal rests and rest seats of 184.91 μm (95% CI: 152.6 μm-217.15 μm) and heterogeneity (I Conclusions: Clinical studies and in vitro research on CAD-CAM planning and manufacturing of removable prosthesis frameworks are still sparse. However, preliminary data indicate a similar fit and esthetic improvement when compared with the conventional technique.
MeSH term(s)	Benzophenones ; Chromium ; Cobalt ; Computer-Aided Design ; Computers ; Denture, Partial, Removable ; Esthetics, Dental ; Humans ; Polymers ; Technology
Chemical Substances	Benzophenones ; Polymers ; Chromium (0R0008Q3JB) ; polyetheretherketone (31694-16-3) ; Cobalt (3G0H8C9362)
Language	English
Publishing date	2021-02-11
Publishing country	United States
Document type	Journal Article ; Meta-Analysis ; Review ; Systematic Review
ZDB-ID	218157-5
ISSN	1097-6841 ; 0022-3913
ISSN (online)	1097-6841
ISSN	0022-3913
DOI	10.1016/j.prosdent.2020.06.006
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Article: Development of Piezoresistive Sensors Based on Graphene Nanoplatelets Screen-Printed on Woven and Knitted Fabrics: Optimisation of Active Layer Formulation and Transversal/Longitudinal Textile Direction.

Arruda, Luisa M / Moreira, Inês P / Sanivada, Usha Kiran / Carvalho, Helder / Fangueiro, Raul

Materials (Basel, Switzerland)

2022 Volume 15, Issue 15

Abstract: ... Therefore, the objective of this study was to investigate the influence of the fabric orientation on both knitted and woven ... physical properties of these screen-printed fabrics were analysed using Field Emission Scanning Electron Microscopy ...

Abstract	Although the force/pressure applied onto a textile substrate through a uniaxial compression is constant and independent of the yarn direction, it should be noted that such mechanical action causes a geometric change in the substrate, which can be identified by the reduction in its lateral thickness. Therefore, the objective of this study was to investigate the influence of the fabric orientation on both knitted and woven pressure sensors, in order to generate knowledge for a better design process during textile piezoresistive sensor development. For this purpose, these distinct textile structures were doped with different concentrations of graphene nanoplatelets (GNPs), using the screen-printing technique. The chemical and physical properties of these screen-printed fabrics were analysed using Field Emission Scanning Electron Microscopy, Ground State Diffuse Reflectance and Raman Spectroscopy. Samples were subjected to tests determining linear electrical surface resistance and piezoresistive behaviour. In the results, a higher presence of conductive material was found in woven structures. For the doped samples, the electrical resistance varied between 10
Language	English
Publishing date	2022-07-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma15155185
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Article ; Online: ViralFP: A Web Application of Viral Fusion Proteins.

Moreira, Pedro / Sequeira, Ana Marta / Pereira, Sara / Rodrigues, Rúben / Rocha, Miguel / Lousa, Diana

Frontiers in medical technology

2021 Volume 3, Page(s) 722392

Abstract: ... available through a free-to-use web server accessible through the URL https://viralfp.bio.di.uminho.pt ... This web application contains several bioinformatic tools, such as Clustal sequence alignment and Weblogo ... of our immune system and their potential as therapeutic targets, this web application aims to foster our ability ...

Abstract	Viral fusion proteins are attached to the membrane of enveloped viruses (a group that includes Coronaviruses, Dengue, HIV and Influenza) and catalyze fusion between the viral and host membranes, enabling the virus to insert its genetic material into the host cell. Given the importance of these biomolecules, this work presents a centralized database containing the most relevant information on viral fusion proteins, available through a free-to-use web server accessible through the URL https://viralfp.bio.di.uminho.pt/. This web application contains several bioinformatic tools, such as Clustal sequence alignment and Weblogo, including as well a machine learning-based tool capable of predicting the location of fusion peptides (the component of fusion proteins that inserts into the host's cell membrane) within the fusion protein sequence. Given the crucial role of these proteins in viral infection, their importance as natural targets of our immune system and their potential as therapeutic targets, this web application aims to foster our ability to fight pathogenic viruses.
Language	English
Publishing date	2021-08-23
Publishing country	Switzerland
Document type	Journal Article
ISSN	2673-3129
ISSN (online)	2673-3129
DOI	10.3389/fmedt.2021.722392
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Article ; Online: Evaluating the Feasibility of Emotion Expressions in Avatars Created From Real Person Photos: Pilot Web-Based Survey of Virtual Reality Software.

Dechsling, Anders / Cogo-Moreira, Hugo / Gangestad, Jonathan Spydevold / Johannessen, Sandra Nettum / Nordahl-Hansen, Anders

JMIR formative research

2023 Volume 7, Page(s) e44632

Abstract: ... on the web containing 27 different images of avatars transformed, using a software, from real images ...

Abstract	Background: The availability and potential of virtual reality (VR) has led to an increase of its application. VR is suggested to be helpful in training elements of social competence but with an emphasis on interventions being tailored. Recognizing facial expressions is an important social skill and thus a target for training. Using VR in training these skills could have advantages over desktop alternatives. Children with autism, for instance, appear to prefer avatars over real images when assessing facial expressions. Available software provides the opportunity to transform profile pictures into avatars, thereby giving the possibility of tailoring according to an individual's own environment. However, the emotions provided by such software should be validated before application. Objective: Our aim was to investigate whether available software is a quick, easy, and viable way of providing emotion expressions in avatars transformed from real images. Methods: A total of 401 participants from a general population completed a survey on the web containing 27 different images of avatars transformed, using a software, from real images. We calculated the reliability of each image and their level of difficulty using a structural equation modeling approach. We used Bayesian confirmatory factor analysis testing under a multidimensional first-order correlated factor structure where faces showing the same emotions represented a latent variable. Results: Few emotions were correctly perceived and rated as higher than other emotions. The factor loadings indicating the discrimination of the image were around 0.7, which means 49% shared variance with the latent factor that the face is linked with. The standardized thresholds indicating the difficulty level of the images are mostly around average, and the highest correlation is between faces showing happiness and anger. Conclusions: Only using a software to transform profile pictures to avatars is not sufficient to provide valid emotion expressions. Adjustments are needed to increase faces' discrimination (eg, increasing reliabilities). The faces showed average levels of difficulty, meaning that they are neither very difficult nor very easy to perceive, which fits a general population. Adjustments should be made for specific populations and when applying this technology in clinical practice.
Language	English
Publishing date	2023-05-11
Publishing country	Canada
Document type	Journal Article
ISSN	2561-326X
ISSN (online)	2561-326X
DOI	10.2196/44632
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Article: IMPACT OF FABRICS FROM TRANSGENIC FLAX PLANT ON HUMAN DERMAL FIBROBLASTS IN VITRO PROLIFERATION.

Gebarowski, Tomasz / Moreira, Helena / Szyjka, Anna / Wiatrak, Benita / Wojtasik, Wioletta / Kulma, Anna / Szopa, Jan / Gasiorowski, Kazimierz

Acta poloniae pharmaceutica

2017 Volume 74, Issue 2, Page(s) 642–652

Abstract: ... of beneficial action of transgenic flax fabrics on wound healing, in the present study the impact of flax fabric ... was investigated. NHDF cell cultures were exposed for 48 h to specific area of flax fabric cuts, made ... to harvested cell cultures). None of the tested flax fabrics were cytotoxic to fibroblast cultures and also did ...

Abstract	Previously it was documented that transgenic flax plants, which contained an increased level of polyphenolic compounds, significantly improved healing of skin wounds lesions. In order to recognize mechanisms of beneficial action of transgenic flax fabrics on wound healing, in the present study the impact of flax fabric pieces/cuts from three types of transgenic flax on normal human dermal fibroblasts primary culture (NHDF) was investigated. NHDF cell cultures were exposed for 48 h to specific area of flax fabric cuts, made from M50, B 14 and M50+B14 (intertwined fibers of M and B), or parallely, extracts from fibers of the tested flax materials to cell culture medium. Cultures were inspected for cell viability, proliferation, cell cycle changes and for their resistance to oxidative stress (consecutive addition of H₂,O₂, to harvested cell cultures). None of the tested flax fabrics were cytotoxic to fibroblast cultures and also did not increase significantly a frequency of apoptotic cells in cultures. In the comet assay, the tested flax fabrics revealed significant protective effect on DNA damage ciused by addition of H₂0₂ to the cultures at the end of incubation time. Fabrics from transgenic flax significantly enhanced fibroblasts proliferation in vitro estimated with the SRB test. Flow cytometric analysis revealed higher frequency of cells in the S phase, in the presence of transgenic flax fabrics. Fabrics from B14 and M50+B14 flax are the most potent activators of NHDF cells in applied in vityo tests, hence they could be recommended for elaboration of new type bandage, able to improve skin wound healing.
MeSH term(s)	Animals ; Apoptosis/drug effects ; BALB 3T3 Cells ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; DNA Damage/drug effects ; Fibroblasts/drug effects ; Fibroblasts/metabolism ; Fibroblasts/pathology ; Flax/enzymology ; Flax/genetics ; Flax/growth & development ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Plant ; Humans ; Hydrogen Peroxide/toxicity ; Mice ; Oxidative Stress/drug effects ; Plant Preparations/pharmacology ; Plant Preparations/toxicity ; Plants, Genetically Modified/enzymology ; Plants, Genetically Modified/genetics ; Plants, Genetically Modified/growth & development ; S Phase/drug effects ; Textiles ; Time Factors ; Wound Healing/drug effects
Chemical Substances	Plant Preparations ; Hydrogen Peroxide (BBX060AN9V)
Language	English
Publishing date	2017-03
Publishing country	Poland
Document type	Journal Article
ZDB-ID	40045-2
ISSN	0001-6837
ISSN	0001-6837
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Article ; Online: V79 Fibroblasts Are Protected Against Reactive Oxygen Species by Flax Fabric.

Skórkowska-Telichowska, Katarzyna / Kulma, Anna / Gębarowski, Tomasz / Wojtasik, Wioleta / Kostyn, Kamil / Moreira, Helena / Szyjka, Anna / Boba, Aleksandra / Preisner, Marta / Mierziak, Justyna / Arendt, Malgorzata / Kostyn, Anna / Szatkowski, Michał / Szopa, Jan / Gąsiorowski, Kazimierz

Applied biochemistry and biotechnology

2017 Volume 184, Issue 1, Page(s) 366–385

Abstract: Chinese hamster pulmonary fibroblasts (V79 cells) pre-treated with flax fabrics derived from non ...

Abstract	Chinese hamster pulmonary fibroblasts (V79 cells) pre-treated with flax fabrics derived from non-modified or genetically engineered flax fibres and treated with H
MeSH term(s)	Animals ; Cell Line ; Comet Assay ; Cricetinae ; Fibroblasts/drug effects ; Flax ; Plants, Genetically Modified ; Reactive Oxygen Species/metabolism
Chemical Substances	Reactive Oxygen Species
Language	English
Publishing date	2017-07-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	392344-7
ISSN	1559-0291 ; 0273-2289
ISSN (online)	1559-0291
ISSN	0273-2289
DOI	10.1007/s12010-017-2552-y
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Article ; Online: A precision overview of genomic resistance screening in Ecuadorian isolates of Mycobacterium tuberculosis using web-based bioinformatics tools.

Morey-León, Gabriel / Mejía-Ponce, Paulina M / Granda Pardo, Juan Carlos / Muñoz-Mawyin, Karen / Fernández-Cadena, Juan Carlos / García-Moreira, Evelyn / Andrade-Molina, Derly / Licona-Cassani, Cuauhtémoc / Berná, Luisa

PloS one

2023 Volume 18, Issue 12, Page(s) e0294670

Abstract: ... of web-based tools, including Mykrobe, TB-profiler, PhyResSE, KvarQ, and SAM-TB, for detecting resistance ... We also identified that in our study, only 50% of mutations characterized by the web-based tools ... and identifying a more efficient and user-friendly web-based tool to characterize resistance ...

Abstract	Introduction: Tuberculosis (TB) is among the deadliest diseases worldwide, and its impact is mainly due to the continuous emergence of resistant isolates during treatment due to the laborious process of resistance diagnosis, nonadherence to treatment and circulation of previously resistant isolates of Mycobacterium tuberculosis. In this study, we evaluated the performance and functionalities of web-based tools, including Mykrobe, TB-profiler, PhyResSE, KvarQ, and SAM-TB, for detecting resistance in 88 Ecuadorian isolates of Mycobacterium tuberculosis drug susceptibility tested previously. Statistical analysis was used to determine the correlation between genomic and phenotypic analysis. Our results showed that with the exception of KvarQ, all tools had the highest correlation with the conventional drug susceptibility test (DST) for global resistance detection (98% agreement and 0.941 Cohen's kappa), while SAM-TB, PhyResSE, TB-profiler and Mykrobe had better correlations with DST for first-line drug analysis individually. We also identified that in our study, only 50% of mutations characterized by the web-based tools in the rpoB, katG, embB, pncA, gyrA and rrs regions were canonical and included in the World Health Organization (WHO) catalogue. Our findings suggest that SAM-TB, PhyResSE, TB-profiler and Mykrobe were efficient in determining canonical resistance-related mutations, but more analysis is needed to improve second-line detection. Improving surveillance programs using whole-genome sequencing tools for first-line drugs, MDR-TB and XDR-TB is essential to understand the molecular epidemiology of TB in Ecuador. Importance: Tuberculosis, an infectious disease caused by Mycobacterium tuberculosis, most commonly affects the lungs and is often spread through the air when infected people cough, sneeze, or spit. However, despite the existence of effective drug treatment, patient adherence, long duration of treatment, and late diagnosis have reduced the effectiveness of therapy and increased drug resistance. The increase in resistant cases, added to the impact of the COVID-19 pandemic, has highlighted the importance of implementing efficient and timely diagnostic methodologies worldwide. The significance of our research is in evaluating and identifying a more efficient and user-friendly web-based tool to characterize resistance in Mycobacterium tuberculosis by whole-genome sequencing, which will allow more routine application to improve TB strain surveillance programs locally.
MeSH term(s)	Humans ; Mycobacterium tuberculosis ; Antitubercular Agents/pharmacology ; Antitubercular Agents/therapeutic use ; Ecuador/epidemiology ; Pandemics ; Tuberculosis, Multidrug-Resistant/diagnosis ; Tuberculosis, Multidrug-Resistant/drug therapy ; Tuberculosis, Multidrug-Resistant/epidemiology ; Computational Biology ; Genomics ; Mutation ; Microbial Sensitivity Tests ; Internet ; Drug Resistance, Multiple, Bacterial/genetics
Chemical Substances	Antitubercular Agents
Language	English
Publishing date	2023-12-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0294670
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