LIVIVO - Search results -

Search results

Result 1 - 10 of total 142741

Article: p

Smith, Megan D / Tassoulas, Lambros J / Biernath, Troy A / Richman, Jack E / Aukema, Kelly G / Wackett, Lawrence P

Computational and structural biotechnology journal

2021 Volume 19, Page(s) 3087–3096

Abstract: The OleA enzyme is distinct amongst thiolase enzymes in binding two long (≥ ... ...

Abstract	The OleA enzyme is distinct amongst thiolase enzymes in binding two long (≥C
Language	English
Publishing date	2021-05-21
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2694435-2
ISSN	2001-0370
ISSN	2001-0370
DOI	10.1016/j.csbj.2021.05.031
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- Full text online
- Order with fees

Book ; Online: Constraining the Astrophysical p Process

Palmisano-Kyle, Alicia / Spyrou, Artemis / DeYoung, Paul / Gastis, Panagiotis / Olivas-Gomez, Orlando / Harris, Caley / Liddick, Sean / Lyons, Stephanie / Pereira, Jorge / Richard, Andrea / Simon, Anna / Smith, Mallory / Tsantiri, Artemis / Zegers, Remco

Cross Section Measurement of the 84Kr(p,g)85Rb Reaction in Inverse Kinematics

2023

Abstract: ... from and how they are made. This work focuses on the p process, a nucleosynthesis process that consists ... of stability. These nuclei, known as the p nuclei, cannot be made through the well-known neutron-capture ... processes. Currently p-process models rely heavily on theory to provide the relevant reaction rates ...

Abstract	One of the biggest questions in nuclear astrophysics is understanding where the elements come from and how they are made. This work focuses on the p process, a nucleosynthesis process that consists of a series of photodisintegration reactions responsible for producing stable isotopes on the proton-rich side of stability. These nuclei, known as the p nuclei, cannot be made through the well-known neutron-capture processes. Currently p-process models rely heavily on theory to provide the relevant reaction rates to predict the final p-nuclei abundances and more experimental data is needed. The present work reports on an experiment performed with the SuN detector at the National Superconducting Cyclotron Laboratory, NSCL, at Michigan State University using the ReA facility to measure the $^{84}$Kr(p,$\gamma$)$^{85}$Rb reaction cross section in inverse kinematics. The reverse $^{85}$Rb($\gamma$,p)$^{84}$Kr reaction is a branching point in the p-process reaction network that was highlighted as an important reaction in sensitivity studies in the production of the $^{78}$Kr p nucleus. A new hydrogen gas target was designed and fabricated and a new analysis technique for background subtraction and efficiency calculations of the detector were developed. The experimental cross section is compared to standard statistical model calculations using the NON-SMOKER and TALYS codes.
Keywords	Nuclear Experiment
Subject code	660
Publishing date	2023-03-13
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: High-performance p-type V

Ainabayev, Ardak / Walls, Brian / Mullarkey, Daragh / Caffrey, David / Fleischer, Karsten / Smith, Christopher M / McGlinchey, Amy / Casey, Daniel / McCormack, Sarah J / Shvets, Igor

Scientific reports

2024 Volume 14, Issue 1, Page(s) 1928

Abstract: High-quality epitaxial p-type V ...

Abstract	High-quality epitaxial p-type V
Language	English
Publishing date	2024-01-22
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-52024-4
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Mapping of RNase P Ribozyme Regions in Proximity with a Human RNase P Subunit Protein Using Fe(II)-EDTA Cleavage and Nuclease Footprint Analyses.

Trang, Phong / Smith, Adam / Liu, Fenyong

Methods in molecular biology (Clifton, N.J.)

2023 Volume 2666, Page(s) 55–67

Abstract: Ribonuclease P (RNase P), which may consist of both protein subunits and a catalytic RNA part, is ... responsible for 5' maturation of tRNA by cleaving the 5'-leader sequence. In Escherichia coli, RNase P ... contains a catalytic RNA subunit (M1 RNA) and a protein factor (C5 protein). In human cells, RNase P ...

Abstract	Ribonuclease P (RNase P), which may consist of both protein subunits and a catalytic RNA part, is responsible for 5' maturation of tRNA by cleaving the 5'-leader sequence. In Escherichia coli, RNase P contains a catalytic RNA subunit (M1 RNA) and a protein factor (C5 protein). In human cells, RNase P holoenzyme consists of an RNA subunit (H1 RNA) and multiple protein subunits that include human RPP29 protein. M1GS, a sequence specific targeting ribozyme derived from M1 RNA, can be constructed to target a specific mRNA to degrade it in vitro. Recent studies have shown that M1GS ribozymes are efficient in blocking the expression of viral mRNAs in cultured cells and in animals. These results suggest that RNase P ribozymes have the potential to be useful in basic research and in clinical applications. It has been shown that RNase P binding proteins, such as C5 protein and RPP29, can enhance the activities of M1GS RNA in processing a natural tRNA substrate and a target mRNA. Understanding how RPP29 binds to M1GS RNA and enhances the enzyme's catalytic activity will provide great insight into developing more robust gene-targeting ribozymes for in vivo application. In this chapter, we describe the methods of using Fe(II)-ethylenediaminetetraacetic acid (EDTA) cleavage and nuclease footprint analyses to determine the regions of a M1GS ribozyme that are in proximity to RPP29 protein.
MeSH term(s)	Animals ; Humans ; Ribonuclease P/genetics ; Ribonuclease P/metabolism ; RNA, Catalytic/metabolism ; Edetic Acid ; Protein Subunits/metabolism ; RNA/chemistry ; RNA, Messenger/genetics ; Escherichia coli/metabolism ; Endonucleases/metabolism
Chemical Substances	Ribonuclease P (EC 3.1.26.5) ; RNA, Catalytic ; Fe(II)-EDTA (15651-72-6) ; Edetic Acid (9G34HU7RV0) ; Protein Subunits ; RNA (63231-63-0) ; RNA, Messenger ; Endonucleases (EC 3.1.-)
Language	English
Publishing date	2023-05-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-3191-1_4
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article ; Online: Comparison of the PCPLC Database to NSQIP-P: A Patient Matched Comparison of Surgical Complications Following Repair of Anorectal Malformation.

McNevin, Kathryn / Nicassio, Lauren / Rice-Townsend, Samuel E / Katz, Cindy B / Goldin, Adam / Avansino, Jeffrey / Calkins, Casey M / Durham, Megan M / Page, Kent / Ralls, Matthew W / Reeder, Ron W / Rentea, Rebecca M / Rollins, Michael D / Saadai, Payam / Wood, Richard J / van Leeuwen, Kathleen D / Smith, Caitlin A

Journal of pediatric surgery

2024 Volume 59, Issue 5, Page(s) 997–1002

Abstract: ... outcomes identified in the PCPLC registry with the NSQIP-P database for patients undergoing surgical repair ... participating in NSQIP-P who underwent ARM repair younger than 12 months old were reviewed for 30-day ... complications. These patients were matched to their NSQIP-P record using their hospital identification number ...

Abstract	Background: Anorectal malformations (ARM) are rare and heterogenous which creates a challenge in conducting research and offering recommendations for best practice. The Pediatric Colorectal and Pelvic Learning Consortium (PCPLC) was formed in 2016 to address this challenge and created a shared national data registry to collect information about pediatric colorectal patients. There has been no external validation of the data collected. We sought to evaluate the database by performing a patient matched analysis comparing 30-day outcomes identified in the PCPLC registry with the NSQIP-P database for patients undergoing surgical repair of ARM. Methods: Patients captured in the PCPLC database from 2016 to 2021 at institutions also participating in NSQIP-P who underwent ARM repair younger than 12 months old were reviewed for 30-day complications. These patients were matched to their NSQIP-P record using their hospital identification number, and records were compared for concordance in identified complications. Results: A total of 591 patient records met inclusion criteria in the PCPLC database. Of these, 180 patients were also reviewed by NSQIP-P. One hundred and fifty-six patient records had no complications recorded. Twenty-four patient records had a complication listed in one or both databases. There was a 91 % concordance rate between databases. When excluding complications not tracked in the PCPLC registry, this agreement improved to 93 %. Conclusion: Including all patients evaluated for this subpopulation, a 91 % concordance rate was observed when comparing PCPLC collected complications to NSQIP-P. Future efforts can focus on further validating the data within the PCPLC for other patient populations. Level of evidence: V.
MeSH term(s)	Humans ; Child ; Infant ; Postoperative Complications/epidemiology ; Postoperative Complications/etiology ; Anorectal Malformations/surgery ; Anorectal Malformations/complications ; Registries ; Databases, Factual ; Colorectal Neoplasms/complications ; Quality Improvement ; Retrospective Studies
Language	English
Publishing date	2024-01-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	80165-3
ISSN	1531-5037 ; 0022-3468
ISSN (online)	1531-5037
ISSN	0022-3468
DOI	10.1016/j.jpedsurg.2024.01.004
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 607: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: The Precision Between Transcutaneous Carbon Dioxide Versus P

Garlapati, Pranav / Vali, Payam / Lakshminrusimha, Satyan / Smith, Brian J / Zavorsky, Gerald S

Respiratory care

2024 Volume 69, Issue 3, Page(s) 339–344

Abstract: ... hypothermia and high-frequency ventilation. Fluctuations in P: Methods: This was a retrospective chart ... A range of 2-27 simultaneous P: Results: A linear mixed-effect model demonstrated that P: Conclusions ... The neonates' P ...

Abstract	Background: Infants with hypoxic-ischemic encephalopathy are often treated with therapeutic hypothermia and high-frequency ventilation. Fluctuations in P Methods: This was a retrospective chart review of 10 neonates who underwent therapeutic hypothermia requiring respiratory support over 2 y. A range of 2-27 simultaneous P Results: A linear mixed-effect model demonstrated that P Conclusions: The neonates' P
MeSH term(s)	Infant, Newborn ; Humans ; Carbon Dioxide ; Blood Gas Monitoring, Transcutaneous ; Retrospective Studies ; Partial Pressure ; Hypothermia, Induced
Chemical Substances	Carbon Dioxide (142M471B3J)
Language	English
Publishing date	2024-02-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	603252-7
ISSN	1943-3654 ; 0098-9142 ; 0020-1324
ISSN (online)	1943-3654
ISSN	0098-9142 ; 0020-1324
DOI	10.4187/respcare.10696
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 2285: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Reply to Figueira et al.: Can NAD(P)

Smith, Cody D / Schmidt, Cameron A / Fisher-Wellman, Kelsey H / Neufer, P Darrell

The Journal of biological chemistry

2021 Volume 296, Page(s) 100378

MeSH term(s)	Energy Metabolism ; Hydrogen Peroxide ; Mitochondria/metabolism ; NAD/metabolism ; NADP Transhydrogenase, AB-Specific/metabolism
Chemical Substances	NAD (0U46U6E8UK) ; Hydrogen Peroxide (BBX060AN9V) ; NADP Transhydrogenase, AB-Specific (EC 1.6.1.2)
Language	English
Publishing date	2021-03-15
Publishing country	United States
Document type	Letter ; Comment
ZDB-ID	2997-x
ISSN	1083-351X ; 0021-9258
ISSN (online)	1083-351X
ISSN	0021-9258
DOI	10.1016/j.jbc.2021.100378
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Uc I Zs.108: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Plasma p-tau181/Aβ

Fowler, Christopher J / Stoops, Erik / Rainey-Smith, Stephanie R / Vanmechelen, Eugeen / Vanbrabant, Jeroen / Dewit, Nele / Mauroo, Kimberley / Maruff, Paul / Rowe, Christopher C / Fripp, Jurgen / Li, Qiao-Xin / Bourgeat, Pierrick / Collins, Steven J / Martins, Ralph N / Masters, Colin L / Doecke, James D

Alzheimer's & dementia (Amsterdam, Netherlands)

2022 Volume 14, Issue 1, Page(s) e12375

Abstract: ... study.: Results: The p-tau181/Aβ: Discussion: Plasma p-tau181/Aβ ...

Abstract	Background: In Alzheimer's disease (AD), plasma amyloid beta (Aβ) Methods: Prototype Simoa assays were used to measure plasma samples from participants who were either cognitively normal (CN) or had mild cognitive impairment (MCI)/AD in the Australian Imaging, Biomarkers and Lifestyle (AIBL) study. Results: The p-tau181/Aβ Discussion: Plasma p-tau181/Aβ
Language	English
Publishing date	2022-11-25
Publishing country	United States
Document type	Journal Article
ZDB-ID	2832898-X
ISSN	2352-8729
ISSN	2352-8729
DOI	10.1002/dad2.12375
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

Article ; Online: Measurement of Direct-Photon Cross Section and Double-Helicity Asymmetry at sqrt[s]=510 GeV in p[over →]+p[over →] Collisions.

Abdulameer, N J / Acharya, U / Adare, A / Aidala, C / Ajitanand, N N / Akiba, Y / Akimoto, R / Alfred, M / Apadula, N / Aramaki, Y / Asano, H / Atomssa, E T / Awes, T C / Azmoun, B / Babintsev, V / Bai, M / Bandara, N S / Bannier, B / Barish, K N /

Bathe, S / Bazilevsky, A / Beaumier, M / Beckman, S / Belmont, R / Berdnikov, A / Berdnikov, Y / Bichon, L / Black, D / Blankenship, B / Bok, J S / Borisov, V / Boyle, K / Brooks, M L / Bryslawskyj, J / Buesching, H / Bumazhnov, V / Campbell, S / Canoa Roman, V / Chen, C-H / Chiu, M / Chi, C Y / Choi, I J / Choi, J B / Chujo, T / Citron, Z / Connors, M / Corliss, R / Corrales Morales, Y / Csanád, M / Csörgő, T / Datta, A / Daugherity, M S / David, G / Dean, C T / DeBlasio, K / Dehmelt, K / Denisov, A / Deshpande, A / Desmond, E J / Ding, L / Dion, A / Doomra, V / Do, J H / Drees, A / Drees, K A / Durham, J M / Durum, A / En'yo, H / Enokizono, A / Esha, R / Fadem, B / Fan, W / Feege, N / Fields, D E / Finger, M / Firak, D / Fitzgerald, D / Fokin, S L / Frantz, J E / Franz, A / Frawley, A D / Gallus, P / Gal, C / Garg, P / Ge, H / Giles, M / Giordano, F / Glenn, A / Goto, Y / Grau, N / Greene, S V / Grosse Perdekamp, M / Gunji, T / Guragain, H / Gu, Y / Hachiya, T / Haggerty, J S / Hahn, K I / Hamagaki, H / Hanks, J / Han, S Y / Harvey, M / Hasegawa, S / Hemmick, T K / He, X / Hill, J C / Hodges, A / Hollis, R S / Homma, K / Hong, B / Hoshino, T / Huang, J / Ikeda, Y / Imai, K / Imazu, Y / Inaba, M / Iordanova, A / Isenhower, D / Ivanishchev, D / Jacak, B V / Jeon, S J / Jezghani, M / Jiang, X / Ji, Z / Johnson, B M / Joo, E / Joo, K S / Jouan, D / Jumper, D S / Kang, J H / Kang, J S / Kawall, D / Kazantsev, A V / Key, J A / Khachatryan, V / Khanzadeev, A / Khatiwada, A / Kihara, K / Kim, C / Kim, D H / Kim, D J / Kim, E-J / Kim, H-J / Kim, M / Kim, T / Kim, Y K / Kincses, D / Kingan, A / Kistenev, E / Klatsky, J / Kleinjan, D / Kline, P / Koblesky, T / Kofarago, M / Koster, J / Kotov, D / Kovacs, L / Kurgyis, B / Kurita, K / Kurosawa, M / Kwon, Y / Lajoie, J G / Larionova, D / Lebedev, A / Lee, K B / Lee, S H / Leitch, M J / Leitgab, M / Lewis, N A / Lim, S H / Liu, M X / Li, X / Loomis, D A / Lynch, D / Lökös, S / Majoros, T / Makdisi, Y I / Makek, M / Manion, A / Manko, V I / Mannel, E / McCumber, M / McGaughey, P L / McGlinchey, D / McKinney, C / Meles, A / Mendoza, M / Meredith, B / Miake, Y / Mignerey, A C / Miller, A J / Milov, A / Mishra, D K / Mitchell, J T / Mitrankova, M / Mitrankov, Iu / Miyasaka, S / Mizuno, S / Mondal, M M / Montuenga, P / Moon, T / Morrison, D P / Moukhanova, T V / Muhammad, A / Mulilo, B / Murakami, T / Murata, J / Mwai, A / Nagamiya, S / Nagle, J L / Nagy, M I / Nakagawa, I / Nakagomi, H / Nakano, K / Nattrass, C / Nelson, S / Netrakanti, P K / Nihashi, M / Niida, T / Nouicer, R / Novitzky, N / Nukazuka, G / Nyanin, A S / O'Brien, E / Ogilvie, C A / Oh, J / Orjuela Koop, J D / Orosz, M / Osborn, J D / Oskarsson, A / Ozawa, K / Pak, R / Pantuev, V / Papavassiliou, V / Park, J S / Park, S / Patel, L / Patel, M / Pate, S F / Peng, J-C / Peng, W / Perepelitsa, D V / Perera, G D N / Peressounko, D Yu / PerezLara, C E / Perry, J / Petti, R / Pinkenburg, C / Pinson, R / Pisani, R P / Potekhin, M / Pun, A / Purschke, M L / Radzevich, P V / Rak, J / Ramasubramanian, N / Ravinovich, I / Read, K F / Reynolds, D / Riabov, V / Riabov, Y / Richford, D / Riveli, N / Roach, D / Rolnick, S D / Rosati, M / Rowan, Z / Rubin, J G / Runchey, J / Saito, N / Sakaguchi, T / Sako, H / Samsonov, V / Sarsour, M / Sato, S / Sawada, S / Schaefer, B / Schmoll, B K / Sedgwick, K / Seele, J / Seidl, R / Sen, A / Seto, R / Sett, P / Sexton, A / Sharma, D / Shein, I / Shibata, M / Shibata, T-A / Shigaki, K / Shimomura, M / Shi, Z / Shukla, P / Sickles, A / Silva, C L / Silvermyr, D / Singh, B K / Singh, C P / Singh, V / Slunečka, M / Smith, K L / Soltz, R A / Sondheim, W E / Sorensen, S P / Sourikova, I V / Stankus, P W / Stepanov, M / Stoll, S P / Sugitate, T / Sukhanov, A / Sumita, T / Sun, J / Sun, Z / Sziklai, J / Takahama, R / Takahara, A / Taketani, A / Tanida, K / Tannenbaum, M J / Tarafdar, S / Taranenko, A / Timilsina, A / Todoroki, T / Tomášek, M / Torii, H / Towell, M / Towell, R / Towell, R S / Tserruya, I / Ueda, Y / Ujvari, B / van Hecke, H W / Vargyas, M / Velkovska, J / Virius, M / Vrba, V / Vznuzdaev, E / Wang, X R / Wang, Z / Watanabe, D / Watanabe, Y / Watanabe, Y S / Wei, F / Whitaker, S / Wolin, S / Wong, C P / Woody, C L / Wysocki, M / Xia, B / Xue, L / Yalcin, S / Yamaguchi, Y L / Yanovich, A / Yoon, I / Younus, I / Yushmanov, I E / Zajc, W A / Zelenski, A / Zou, L

Physical review letters

2023 Volume 130, Issue 25, Page(s) 251901

Abstract: ... production in p[over →]+p[over →] collisions at sqrt[s]=510 GeV. The measurements have been performed ...

Abstract	We present measurements of the cross section and double-helicity asymmetry A_{LL} of direct-photon production in p[over →]+p[over →] collisions at sqrt[s]=510 GeV. The measurements have been performed at midrapidity (\|η\|<0.25) with the PHENIX detector at the Relativistic Heavy Ion Collider. At relativistic energies, direct photons are dominantly produced from the initial quark-gluon hard scattering and do not interact via the strong force at leading order. Therefore, at sqrt[s]=510 GeV, where leading-order-effects dominate, these measurements provide clean and direct access to the gluon helicity in the polarized proton in the gluon-momentum-fraction range 0.02<x<0.08, with direct sensitivity to the sign of the gluon contribution.<br />
MeSH term(s)	Photons ; Food ; Motion
Language	English
Publishing date	2023-07-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	208853-8
ISSN	1079-7114 ; 0031-9007
ISSN (online)	1079-7114
ISSN	0031-9007
DOI	10.1103/PhysRevLett.130.251901
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 4' 58/153: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: p-tau Ser356 is associated with Alzheimer's disease pathology and is lowered in brain slice cultures using the NUAK inhibitor WZ4003.

Taylor, Lewis W / Simzer, Elizabeth M / Pimblett, Claire / Lacey-Solymar, Oscar T T / McGeachan, Robert I / Meftah, Soraya / Rose, Jamie L / Spires-Jones, Maxwell P / Holt, Kristján / Catterson, James H / Koch, Henner / Liaquat, Imran / Clarke, Jonathan H / Skidmore, John / Smith, Colin / Booker, Sam A / Brennan, Paul M / Spires-Jones, Tara L / Durrant, Claire S

Acta neuropathologica

2024 Volume 147, Issue 1, Page(s) 7

Abstract: ... provides a detailed characterisation of the association of p-tau Ser356 with progression ... of Alzheimer's disease pathology, identifying a Braak stage-dependent increase in p-tau Ser356 protein levels and ... resolution array tomography imaging, that p-tau Ser356 co-localises with synapses in AD postmortem brain ...

Abstract	Tau hyperphosphorylation and aggregation is a common feature of many dementia-causing neurodegenerative diseases. Tau can be phosphorylated at up to 85 different sites, and there is increasing interest in whether tau phosphorylation at specific epitopes, by specific kinases, plays an important role in disease progression. The AMP-activated protein kinase (AMPK)-related enzyme NUAK1 has been identified as a potential mediator of tau pathology, whereby NUAK1-mediated phosphorylation of tau at Ser356 prevents the degradation of tau by the proteasome, further exacerbating tau hyperphosphorylation and accumulation. This study provides a detailed characterisation of the association of p-tau Ser356 with progression of Alzheimer's disease pathology, identifying a Braak stage-dependent increase in p-tau Ser356 protein levels and an almost ubiquitous presence in neurofibrillary tangles. We also demonstrate, using sub-diffraction-limit resolution array tomography imaging, that p-tau Ser356 co-localises with synapses in AD postmortem brain tissue, increasing evidence that this form of tau may play important roles in AD progression. To assess the potential impacts of pharmacological NUAK inhibition in an ex vivo system that retains multiple cell types and brain-relevant neuronal architecture, we treated postnatal mouse organotypic brain slice cultures from wildtype or APP/PS1 littermates with the commercially available NUAK1/2 inhibitor WZ4003. Whilst there were no genotype-specific effects, we found that WZ4003 results in a culture-phase-dependent loss of total tau and p-tau Ser356, which corresponds with a reduction in neuronal and synaptic proteins. By contrast, application of WZ4003 to live human brain slice cultures results in a specific lowering of p-tau Ser356, alongside increased neuronal tubulin protein. This work identifies differential responses of postnatal mouse organotypic brain slice cultures and adult human brain slice cultures to NUAK1 inhibition that will be important to consider in future work developing tau-targeting therapeutics for human disease.
MeSH term(s)	Adult ; Humans ; Animals ; Mice ; Alzheimer Disease ; Brain ; Anilides ; Neurofibrillary Tangles ; Protein Kinases ; Repressor Proteins
Chemical Substances	WZ4003 ; Anilides ; NUAK1 protein, human (EC 2.7.1.-) ; Protein Kinases (EC 2.7.-) ; Repressor Proteins
Language	English
Publishing date	2024-01-04
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1079-0
ISSN	1432-0533 ; 0001-6322
ISSN (online)	1432-0533
ISSN	0001-6322
DOI	10.1007/s00401-023-02667-w
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ui II Zs.188: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

Full text online

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito