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Article ; Online: S-nitrosothiols and H

Reis, Adriana Karla Cardoso Amorim / Stern, Arnold / Monteiro, Hugo Pequeno

2019 Volume 27, Page(s) 101190

Abstract: Nitric Oxide (NO) and Hydrogen Sulfide ( ... ...

Abstract	Nitric Oxide (NO) and Hydrogen Sulfide (H
MeSH term(s)	Animals ; Antineoplastic Agents/pharmacology ; Humans ; Hydrogen Sulfide/pharmacology ; Neoplasms/drug therapy ; Neoplasms/metabolism ; Nitric Oxide/metabolism ; Oxidation-Reduction/drug effects ; Protein Processing, Post-Translational/drug effects ; S-Nitrosothiols/pharmacology
Chemical Substances	Antineoplastic Agents ; S-Nitrosothiols ; Nitric Oxide (31C4KY9ESH) ; Hydrogen Sulfide (YY9FVM7NSN)
Language	English
Publishing date	2019-04-05
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2701011-9
ISSN	2213-2317 ; 2213-2317
ISSN (online)	2213-2317
ISSN	2213-2317
DOI	10.1016/j.redox.2019.101190
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Article ; Online: Morphoanatomical study of the species Zanthoxylum kleinii (R.S. Cowan) P.G. Waterman (Rutaceae).

Fladzinski, Karine Amorim / Armstrong, Lorene / Dos Anjos, Cintia Aparecida / da Rocha, Leandro Taborda / Miguel, Obdulio Gomes / Miguel, Marilis Dallarmi / Montrucchio, Deise Prehs / de Fátima Gaspari Dias, Josiane

Microscopy research and technique

2024

Abstract: ... of Zanthoxylum L. are distributed throughout the country, and among them is the species Zanthoxylum kleinii (R.S ...

Abstract	The order Sapindales is comprised of nine families and in Brazil it is represented by six, including Rutaceae Juss., which constitutes the largest group of this order. A variety of species of Zanthoxylum L. are distributed throughout the country, and among them is the species Zanthoxylum kleinii (R.S. Cowan) P.G. Waterman, which is found in the states of Brazil. This study aimed to characterize the morphoanatomy of the leaf, petiole, rachis, and stem of the species Z. kleinii. Histochemical tests were performed, and the sections were visualized under optical and scanning electron microscopy. The analysis showed that the morphoanatomical characteristics of the species are: hypoestomatic leaflets; stomata classified as anomocytic, tetracytic, and anisocytic; dorsiventral mesophyll; cavities that produce a secretion of lipid nature, present in the leaflet, rachis, and petiole; colleters distributed in the leaf; presence of simple non-glandular trichomes in all structures; and prismatic crystals in the petiole. Histochemical tests indicated the presence of phenolic and lipophilic compounds, mucilage, and lignin. With the result of this research, it was possible to identify the nature of the compounds secreted by the secretory structures of the leaves; in addition, the morphoanatomical characterization of Z. kleinii can provide relevant data for future studies for other organs of the species not yet described. Furthermore, contributing concomitantly with data for the genus, in this way, supporting to differentiate them. RESEARCH HIGHLIGHTS: Ultrastructural features observed by microscopic techniques. Calcium oxalate crystals present in the rachis. Microchemical tests confirmed the presence of colleters in the leaflet.
Language	English
Publishing date	2024-03-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	1099714-3
ISSN	1097-0029 ; 1059-910X
ISSN (online)	1097-0029
ISSN	1059-910X
DOI	10.1002/jemt.24564
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Article ; Online: Brazil’s quest for autonomy in Asia

Samuel Conde Amorim / Laura C. Ferreira-Pereira

Revista Brasileira de Política Internacional, Vol 64, Iss

the role of strategic partnerships with China and Japan

2021 Volume 2

Abstract: ... Japan, between 2003 and 2020, and assessing how these privileged rapports contributed to the country’s ...

Abstract	Abstract This article aims at examining the strategic partnerships Brazil developed with China and Japan, between 2003 and 2020, and assessing how these privileged rapports contributed to the country’s quest for autonomy. This will be made through the lenses of Pragmatic Institutionalism and against the backdrop of major developments in global and regional governance that impacted on the Brazilian autonomist project.
Keywords	Autonomy ; Brazil ; China ; Japan ; Pragmatic Institutionalism ; Strategic Partnerships ; Political science ; J ; International relations ; JZ2-6530
Language	English
Publishing date	2021-07-01T00:00:00Z
Publisher	Instituto Brasileiro de Relações Internacionais
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Antifungal effect of (R) and (S)-citronellal enantiomers and their predictive mechanism of action on Candida albicans from voriconazole-resistant onychomycoses.

Gouveia, R G / Oliveira, N R / Andrade-Júnior, F P / Ferreira, R C / Amorim, G M W / Silva, D K F / Duarte, S S / Medeiros, C I S / Oliveira-Filho, A A / Lima, E O

Brazilian journal of biology = Revista brasleira de biologia

2023 Volume 83, Page(s) e271530

Abstract: ... by yeasts. This study investigated the antifungal effect of (R) and (S)-citronellal enantiomers, as well ... resistant to voriconazole and sensitive to the enantiomers (R) and (S)-citronellal at a dose of 256 and 32 ... and (S)-citronellal interacting with two important enzymes: 1,3-β-glucan synthase and lanosterol 14α ...

Abstract	Onychomycosis is the most common disease affecting the nail unit and accounts for at least 50% of all nail diseases. In addition, Candida albicans is responsible for approximately 70% of onychomycoses caused by yeasts. This study investigated the antifungal effect of (R) and (S)-citronellal enantiomers, as well as its predictive mechanism of action on C. albicans from voriconazole-resistant onychomycoses. For this purpose, in vitro broth microdilution and molecular docking techniques were applied in a predictive and complementary manner to the mechanisms of action. The main results of this study indicate that C. albicans was resistant to voriconazole and sensitive to the enantiomers (R) and (S)-citronellal at a dose of 256 and 32 µg/mL respectively. In addition, there was an increase in the minimum inhibitory concentration (MIC) of the enantiomers in the presence of sorbitol and ergosterol, indicating that these molecules possibly affect the integrity of the cell wall and cell membrane of C. albicans. Molecular docking with key biosynthesis proteins and maintenance of the fungal cell wall and plasma membrane demonstrated the possibility of (R) and (S)-citronellal interacting with two important enzymes: 1,3-β-glucan synthase and lanosterol 14α-demethylase. Therefore, the findings of this study indicate that the (R) and (S)-citronellal enantiomers are fungicidal on C. albicans from onychomycoses and probably these substances cause damage to the cell wall and cell membrane of these micro-organisms possibly by interacting with enzymes in the biosynthesis of these fungal structures.
MeSH term(s)	Antifungal Agents ; Voriconazole ; Candida albicans ; Onychomycosis ; Molecular Docking Simulation
Chemical Substances	Antifungal Agents ; Voriconazole (JFU09I87TR) ; citronellal (QB99VZZ7GZ)
Language	English
Publishing date	2023-05-22
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2036257-2
ISSN	1678-4375 ; 1519-6984
ISSN (online)	1678-4375
ISSN	1519-6984
DOI	10.1590/1519-6984.271530
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Article ; Online: Adolescent’s movement behaviors and built environment

Isabella Toledo Caetano / Valter Paulo Neves Miranda / Fernanda Karina dos Santos / Paulo Roberto dos Santos Amorim

BMC Public Health, Vol 21, Iss 1, Pp 1-

a latent class analysis

2021 Volume 14

Abstract: Abstract Background Latent class analysis (LCA) is an alternative and innovative approach to verify the relation of the various combinations of the constructed environment and movement behavior (levels of physical activity, sedentary behavior, and sleep) ...

Abstract	Abstract Background Latent class analysis (LCA) is an alternative and innovative approach to verify the relation of the various combinations of the constructed environment and movement behavior (levels of physical activity, sedentary behavior, and sleep) characteristics. This study aimed to identify latent classes based on the characteristics of the neighborhood environment perceived by adolescents and their association with gender, socioeconomic status (SS), body composition and movement behaviors. Methods This cross-sectional study includes 309 Brazilian adolescents (14 to 16 years old, 57% female). The characteristics of the neighborhood environment perceived were analyzed by the Neighborhood Walkability for Youth Scale. Accelerometers were used for a week to evaluate the movement behaviors. Questionnaires assessed the screen times, total sitting time (TST), and sociodemographic characteristics. LCA was used for modeling the “Perceived Enviroment” variable, having been conducted in the poLCA (Polychromous Variable Latent Class Analysis) package of the R statistical software. Results Three classes were recognized: class 1, “Best Perceived Environment” with 23.03% of adolescents; class 2, “Moderate Perceived Environment”, 63.33%; and class 3, “Worst Perceived Environment”, 13.67%. Light physical activity (LPA), TST, and SS were associated with class prevalence. The adolescents with medium and low SS were, respectively, 3.42 (95% CI 1.62–7.21) and 4.18 (95% CI 1.66–10.50) more likely to belong to class 2, and those with low SS were 5.21 (95% CI 1.35–20.13) more likely to belong to class 3. Class 1 adolescents were associated with a lower chance (OR: 0.09, 95% CI 0.02–0.55) of involvement in ‘adequate LPA time’ compared to class 3. Class 1 adolescents were associated with a lower chance (OR: 0.31, 95% CI 0.12–0.79) of involvement in ‘adequate TST’ compared to class 2. There was a difference between the LPA and TST classes; class 3 presented a longer time in LPA than class 1; class 1 had higher TST than the other ...
Keywords	Built environment ; Neighborhood ; Accelerometer ; Movement behaviors ; Latent class analysis ; Public aspects of medicine ; RA1-1270
Subject code	796
Language	English
Publishing date	2021-10-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Intraspecific variation in short-beaked common dolphin’s whistle repertoire

Pagliani, Bruna / Amorim, Thiago O. S. / De Castro, Franciele R. / Andriolo, Artur

Bioacoustics. 2022 Jan. 02, v. 31, no. 1

2022

Abstract: Dolphins’ whistles are strongly diversified mainly related to physiology and behaviour. We present the whistle characteristics and intraspecific variations of short-beaked common dolphins Delphinus delphis of the Western South Atlantic Ocean (WSAO). A ... ...

Abstract	Dolphins’ whistles are strongly diversified mainly related to physiology and behaviour. We present the whistle characteristics and intraspecific variations of short-beaked common dolphins Delphinus delphis of the Western South Atlantic Ocean (WSAO). A total of 1630 whistles from six encounters were analysed considering group size, contours and acoustical parameters. No significant correlation was found (p > 0.05) between number of whistles and the group size. Upsweep, downsweep and constant were the most frequent contours. A positive relation between the downsweep contour and group size was detected (p < 0.05). Minimum, maximum, centre, peak, initial and final frequencies, frequency range, frequency gradient, duration and inflection points were extracted. The frequencies ranged from 1.34 to 35.23 kHz and the duration varied from 0.11 to 2.16 seconds. Comparing encounters between years through Bonferroni method, minimum frequency, duration, and frequency range were significantly different. C5.0 decision tree showed that whistles of 2014 and 2015 had correct classifications (67.0% and 54.0%, respectively). The nMDS grouped the Atlantic and Pacific oceans based on inflection points and duration, further corroborated through PERMANOVA (p < 0.05). Our results suggest the influence of certain parameters related to intraspecific variation. Whistles comparison with other localities corroborates with differences in geographical scale.
Keywords	Delphinus delphis ; bioacoustics ; decision support systems ; group size ; intraspecific variation ; physiology ; Atlantic Ocean
Language	English
Dates of publication	2022-0102
Size	p. 1-16.
Publishing place	Taylor & Francis
Document type	Article
ISSN	2165-0586
DOI	10.1080/09524622.2020.1858449
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: "Molecular analysis of the rare S-s- red blood cell phenotype in blood donors and patients in south-east Brazil".

Santos, Flávia Leite Souza / Cuter, Talitha Baldin / Rodrigues, Evandra Strazza / Bettarello, Êmile Cristina / Ubiali, Eugenia Maria Amorim / Castilho, Lilian Maria / Kashima, Simone / Covas, Dimas Tadeu

Vox sanguinis

2019 Volume 114, Issue 3, Page(s) 262–267

Abstract: Background and objectives: The rare S-s- phenotype has two main molecular backgrounds. GYPB ... deletions give rise to the S-s-U- phenotype, which loses the expression of the U antigen, while variant GYPB ... alleles usually lead to the S-s-U: Material and methods: Five patients and 25 donors with the S-s ...

Abstract	Background and objectives: The rare S-s- phenotype has two main molecular backgrounds. GYPB deletions give rise to the S-s-U- phenotype, which loses the expression of the U antigen, while variant GYPB alleles usually lead to the S-s-U Material and methods: Five patients and 25 donors with the S-s- phenotype were investigated through real-time PCR for the GYPBS/s polymorphism, followed by an allele-specific/RFLP-PCR for GYPB deletion (GYPBNull) and for its main variants: GYPBP2 and GYPBNY. DNA sequencing was conducted in one sample. Results: Two samples were heterozygous GYPBP2/GYPBNY, eight were homozygous/hemizygous for GYPBP2 and 19 samples were homozygous for GYPBNull. A hybrid gene (GYPB-E-B.Ros) was found in one sample after discrepant results in the initial tests. Conclusion: GYPB deletion is the main mechanism responsible for the S-s- phenotype in our donors and patients. It is essential to evaluate the main GYPB variant alleles when genotyping in order to obtain the correct prediction of the phenotype. Hybrid genes lead to discrepancies between genotype and phenotype and may not be detected by conventional molecular assays.
MeSH term(s)	Blood Donors ; Blood Group Antigens/genetics ; Brazil ; Erythrocytes/metabolism ; Gene Frequency ; Genotype ; Glycophorin/genetics ; Humans ; Phenotype ; Polymorphism, Genetic
Chemical Substances	Blood Group Antigens ; GYPB protein, human ; Glycophorin
Language	English
Publishing date	2019-02-19
Publishing country	England
Document type	Journal Article
ZDB-ID	80313-3
ISSN	1423-0410 ; 0042-9007
ISSN (online)	1423-0410
ISSN	0042-9007
DOI	10.1111/vox.12751
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Article ; Online: S-nitrosothiols and H2S donors

Adriana Karla Cardoso Amorim Reis / Arnold Stern / Hugo Pequeno Monteiro

Redox Biology, Vol 27, Iss , Pp - (2019)

Potential chemo-therapeutic agents in cancer

2019

Abstract: Nitric Oxide (NO) and Hydrogen Sulfide (H2S) are components of an “interactome”, which is defined as a redox system involving the interactions of RSS, RNS and ROS. Chemical interaction by these species is common and is characterized by one and two ... ...

Abstract	Nitric Oxide (NO) and Hydrogen Sulfide (H2S) are components of an “interactome”, which is defined as a redox system involving the interactions of RSS, RNS and ROS. Chemical interaction by these species is common and is characterized by one and two electron oxidation, nitrosylation, nitration and sulfuration/polysulfidation reactions. NO and H2S are gases that penetrate cell membranes, are synthesized by specific enzymes, are ubiquitous, regulate protein activities through post-translational modifications and participate in cell signaling. The two molecules at high concentrations compared to physiological concentrations may result in cellular damage particularly through their interaction with other reactive species. NO and H2S can interact with each other and form a variety of molecular species which may have constructive or destructive behavior depending on the cell type, the cellular environment (ex. oxygen tension, pH, redox state), where the products are produced and in what concentrations. Cross talk exists between NO and H2S, whereby they can influence the generation and signaling behavior of each other. Given the above mentioned properties of NO and H2S and studies in cancer cells and animal models employing NO and H2S donors that generate higher than physiological concentrations of NO and H2S and are effective in killing cancer cells but not normal cells, lend credence to the possibility of the utility of these donors in an approach to the treatment of cancer.
Keywords	Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2019-10-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Successful 3D culture and transplantation of mouse isolated preantral follicles in hydrogel of bioengineered Wharton’s jelly

Elnaz Zand / Elias Rajablou / Seyedeh Fatemeh Siadat / Bahare Beiki / Vahid Akbarinejad / Christiani Andrade Amorim / Mojtaba Rezazadeh Valojerdi / Leila Alsadat Tahaei / Rouhollah Fathi

PLoS ONE, Vol 18, Iss

2023 Volume 9

Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Zika and microcephaly on Fiocruz’s Facebook

Paloma da Silva Barreto / Luís de Amorim / Marcelo Pereira Garcia / Carla Almeida

RECIIS, Vol 14, Iss

the search for dialogue with the population and the action against rumors about the epidemic

2020 Volume 1

Abstract: This article analyzes how Oswaldo Cruz Foundation’s page on Facebook approached the epidemic ...

Abstract	This article analyzes how Oswaldo Cruz Foundation’s page on Facebook approached the epidemic of zika and microcephaly between May 2015 and May 2016. Following procedures of the content analysis, we highlight characteristics of the posts on the topic based on their frequency, frame, format and engagement. We also qualitatively analyze part of the comments of the posts with the highest level of engagement. The results show the great attention received by the theme on the page and bring to evidence the prioritization of the scientific and political-institutional frame, the use of science to confer credibility to the content and the popularity of the posts about rumors among the public. There was also little interaction on the part of Fiocruz and small variation of the formats used in the posts, with prioritization of traditional resources.
Keywords	comunicação e saúde ; divulgação científica ; zika ; microcefalia ; facebook ; Communication. Mass media ; P87-96 ; Public aspects of medicine ; RA1-1270
Language	English
Publishing date	2020-03-01T00:00:00Z
Publisher	Instituto de Comunicação e Informação Científica e Tecnológica em Saúde (Icict) da Fundação Oswaldo Cruz (Fiocruz)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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