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  1. Article ; Online: Development and Characterization of Hybrid, Temperature Sensing and Heating Yarns with Color Change.

    Junge, Theresa / Brendgen, Rike / Grassmann, Carsten / Weide, Thomas / Schwarz-Pfeiffer, Anne

    Sensors (Basel, Switzerland)

    2023  Volume 23, Issue 16

    Abstract: A person's body temperature is an important indicator of their health status. A deviation of that temperature by just 2 °C already has or can lead to serious consequences, such as fever or hypothermia. Hence, the development of a temperature-sensing and ... ...

    Abstract A person's body temperature is an important indicator of their health status. A deviation of that temperature by just 2 °C already has or can lead to serious consequences, such as fever or hypothermia. Hence, the development of a temperature-sensing and heatable yarn is an important step toward enabling and improving the monitoring and regulation of a person's body temperature. This technology offers benefits to several industries, such as health care and sports. This paper focuses on the characterization and development of a hybrid yarn, which can measure and visualize temperature changes through a thermoresistive and thermochromic effect. Moreover, the yarn is able to serve as a flexible heating element by connecting to a power source. The structure of the yarn is designed in three layers. Each layer and component ensures the functionality and flexibility of the yarn and additional compatibility with further processing steps. A flexible stainless steel core was used as the heat-sensitive and heat-conducting material. The layer of polyester wrapped around the stainless steel yarn improves the wearing comfort and serves as substrate material for the thermochromic coating. The resulting hybrid yarn has a reproducible sensory function and changes its resistance by 0.15 Ω between 20 and 60 °C for a length of 30 cm. In addition, the yarn has a uniform and reproducible heating power, so that temperature steps can be achieved at a defined length by selecting certain voltages. The thermochromic color change is clearly visible between 28 and 29 °C. Due to its textile structure, the hybrid sensory and actuating yarn can easily be incorporated into a woven fabric or into a textile by means of joining technology sewing.
    Language English
    Publishing date 2023-08-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2052857-7
    ISSN 1424-8220 ; 1424-8220
    ISSN (online) 1424-8220
    ISSN 1424-8220
    DOI 10.3390/s23167076
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Book ; Online ; Thesis: Untersuchung des Einflusses von Filaminen auf das Aktin-Zytoskelett und die Adhäsivität mechanisch gedehnter Podozyten

    Greiten, Jonas Konstantin [Verfasser] / Endlich, Nicole [Akademischer Betreuer] / Endlich, Nicole [Gutachter] / Weide, Thomas [Gutachter]

    2023  

    Author's details Jonas Konstantin Greiten ; Gutachter: Nicole Endlich, Thomas Weide ; Betreuer: Nicole Endlich
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language German
    Publisher Universität Greifswald
    Publishing place Greifswald
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  3. Book ; Online ; Thesis: Zellbiologische Studien über EPB4.1L5 und seine Beziehung zu Crumbs2 in HEK293T und AB8-Zellen

    Brüning, Klara Kristin [Verfasser] / Weide, Thomas [Akademischer Betreuer]

    2021  

    Author's details Klara Kristin Brüning ; Betreuer: Thomas Weide
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language German
    Publisher Universitäts- und Landesbibliothek Münster
    Publishing place Münster
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  4. Article ; Online: PATJ inhibits histone deacetylase 7 to control tight junction formation and cell polarity.

    Fiedler, Julia / Moennig, Thomas / Hinrichs, Johanna H / Weber, Annika / Wagner, Thomas / Hemmer, Tim / Schröter, Rita / Weide, Thomas / Epting, Daniel / Bergmann, Carsten / Nedvetsky, Pavel / Krahn, Michael P

    Cellular and molecular life sciences : CMLS

    2023  Volume 80, Issue 11, Page(s) 333

    Abstract: The conserved multiple PDZ-domain containing protein PATJ stabilizes the Crumbs-Pals1 complex to regulate apical-basal polarity and tight junction formation in epithelial cells. However, the molecular mechanism of PATJ's function in these processes is ... ...

    Abstract The conserved multiple PDZ-domain containing protein PATJ stabilizes the Crumbs-Pals1 complex to regulate apical-basal polarity and tight junction formation in epithelial cells. However, the molecular mechanism of PATJ's function in these processes is still unclear. In this study, we demonstrate that knockout of PATJ in epithelial cells results in tight junction defects as well as in a disturbed apical-basal polarity and impaired lumen formation in three-dimensional cyst assays. Mechanistically, we found PATJ to associate with and inhibit histone deacetylase 7 (HDAC7). Inhibition or downregulation of HDAC7 restores polarity and lumen formation. Gene expression analysis of PATJ-deficient cells revealed an impaired expression of genes involved in cell junction assembly and membrane organization, which is rescued by the downregulation of HDAC7. Notably, the function of PATJ regulating HDAC7-dependent cilia formation does not depend on its canonical interaction partner, Pals1, indicating a new role of PATJ, which is distinct from its function in the Crumbs complex. By contrast, polarity and lumen phenotypes observed in Pals1- and PATJ-deficient epithelial cells can be rescued by inhibition of HDAC7, suggesting that the main function of this polarity complex in this process is to modulate the transcriptional profile of epithelial cells by inhibiting HDAC7.
    MeSH term(s) Cell Polarity ; Tight Junctions ; Biological Assay ; Down-Regulation ; Histone Deacetylases/genetics
    Chemical Substances Histone Deacetylases (EC 3.5.1.98)
    Language English
    Publishing date 2023-10-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1358415-7
    ISSN 1420-9071 ; 1420-682X
    ISSN (online) 1420-9071
    ISSN 1420-682X
    DOI 10.1007/s00018-023-04994-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: PALS1 is a key regulator of the lateral distribution of tight junction proteins in renal epithelial cells.

    Groh, Ann-Christin / Möller-Kerutt, Annika / Gilhaus, Kevin / Höffken, Verena / Nedvetsky, Pavel / Kleimann, Simon / Behrens, Malina / Ghosh, Sujasha / Hansen, Uwe / Krahn, Michael P / Ebnet, Klaus / Pavenstädt, Hermann / Ludwig, Alexander / Weide, Thomas

    Journal of cell science

    2024  Volume 137, Issue 5

    Abstract: The evolutionarily conserved apical Crumbs (CRB) complex, consisting of the core components CRB3a (an isoform of CRB3), PALS1 and PATJ, plays a key role in epithelial cell-cell contact formation and cell polarization. Recently, we observed that deletion ... ...

    Abstract The evolutionarily conserved apical Crumbs (CRB) complex, consisting of the core components CRB3a (an isoform of CRB3), PALS1 and PATJ, plays a key role in epithelial cell-cell contact formation and cell polarization. Recently, we observed that deletion of one Pals1 allele in mice results in functional haploinsufficiency characterized by renal cysts. Here, to address the role of PALS1 at the cellular level, we generated CRISPR/Cas9-mediated PALS1-knockout MDCKII cell lines. The loss of PALS1 resulted in increased paracellular permeability, indicating an epithelial barrier defect. This defect was associated with a redistribution of several tight junction-associated proteins from bicellular to tricellular contacts. PALS1-dependent localization of tight junction proteins at bicellular junctions required its interaction with PATJ. Importantly, reestablishment of the tight junction belt upon transient F-actin depolymerization or upon Ca2+ removal was strongly delayed in PALS1-deficient cells. Additionally, the cytoskeleton regulator RhoA was redistributed from junctions into the cytosol under PALS1 knockout. Together, our data uncover a critical role of PALS1 in the coupling of tight junction proteins to the F-actin cytoskeleton, which ensures their correct distribution along bicellular junctions and the formation of tight epithelial barrier.
    MeSH term(s) Animals ; Mice ; Actin Cytoskeleton ; Actins ; Cytoskeleton ; Cytosol ; Epithelial Cells ; Tight Junction Proteins ; Nucleoside-Phosphate Kinase/genetics ; Membrane Proteins/genetics
    Chemical Substances Actins ; Tight Junction Proteins ; Mpp5 protein, mouse (EC 2.7.4.8) ; Nucleoside-Phosphate Kinase (EC 2.7.4.4) ; Membrane Proteins
    Language English
    Publishing date 2024-01-24
    Publishing country England
    Document type Journal Article
    ZDB-ID 2993-2
    ISSN 1477-9137 ; 0021-9533
    ISSN (online) 1477-9137
    ISSN 0021-9533
    DOI 10.1242/jcs.261303
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Book ; Online: Checkpoint/Restart for Lagrangian particle mesh with AMR in community code FLASH-X

    Jain, Rajeev / Weide, Klaus / Chawdhary, Saurabh / Klostermann, Thomas

    2021  

    Abstract: In this work we present the design decisions and advantages for accomplishing cross mesh format checkpoint-restart in community code FLASH-X. AMReX and Paramesh are the two AMR mesh formats developed and supported by FLASH-X. We also highlight strong and ...

    Abstract In this work we present the design decisions and advantages for accomplishing cross mesh format checkpoint-restart in community code FLASH-X. AMReX and Paramesh are the two AMR mesh formats developed and supported by FLASH-X. We also highlight strong and weak scaling study of existing HDF5 I/O checkpoint writing along with new ideas and results (presented during talk) for utilizing heterogeneous compute architectures for improved I/O performance.

    Comment: chkpt symposium
    Keywords Computer Science - Distributed ; Parallel ; and Cluster Computing
    Publishing date 2021-03-06
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Loss of surface transport is a main cellular pathomechanism of CRB2 variants causing podocytopathies.

    Möller-Kerutt, Annika / Schönhoff, Birgit / Rellmann, Yvonne / George, Britta / Braun, Daniela Anne / Pavenstädt, Hermann / Weide, Thomas

    Life science alliance

    2022  Volume 6, Issue 3

    Abstract: Crumbs2 (CRB2) is a central component of the renal filtration barrier and part of the slit diaphragm, a unique cell contact formed by glomerular podocytes. ... ...

    Abstract Crumbs2 (CRB2) is a central component of the renal filtration barrier and part of the slit diaphragm, a unique cell contact formed by glomerular podocytes. Some
    MeSH term(s) Humans ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Carrier Proteins/metabolism ; Nephrotic Syndrome/metabolism ; Cell Membrane/metabolism ; Mutation, Missense/genetics
    Chemical Substances Membrane Proteins ; Carrier Proteins ; CRB2 protein, human
    Language English
    Publishing date 2022-12-22
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2575-1077
    ISSN (online) 2575-1077
    DOI 10.26508/lsa.202201649
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Impact of Pals1 on Expression and Localization of Transporters Belonging to the Solute Carrier Family.

    Berghaus, Carmen / Groh, Ann-Christin / Breljak, Davorka / Ciarimboli, Giuliano / Sabolić, Ivan / Pavenstädt, Hermann / Weide, Thomas

    Frontiers in molecular biosciences

    2022  Volume 9, Page(s) 792829

    Abstract: Pals1 is part of the evolutionary conserved Crumbs polarity complex and plays a key role in two processes, the formation of apicobasal polarity and the establishment of cell-cell contacts. In the human kidney, up to 1.5 million nephrons control blood ... ...

    Abstract Pals1 is part of the evolutionary conserved Crumbs polarity complex and plays a key role in two processes, the formation of apicobasal polarity and the establishment of cell-cell contacts. In the human kidney, up to 1.5 million nephrons control blood filtration, as well as resorption and recycling of inorganic and organic ions, sugars, amino acids, peptides, vitamins, water and further metabolites of endogenous and exogenous origin. All nephron segments consist of polarized cells and express high levels of Pals1. Mice that are functionally haploid for Pals1 develop a lethal phenotype, accompanied by heavy proteinuria and the formation of renal cysts. However, on a cellular level, it is still unclear if reduced cell polarization, incomplete cell-cell contact formation, or an altered Pals1-dependent gene expression accounts for the renal phenotype. To address this, we analyzed the transcriptomes of Pals1-haploinsufficient kidneys and the littermate controls by gene set enrichment analysis. Our data elucidated a direct correlation between TGFβ pathway activation and the downregulation of more than 100 members of the solute carrier (SLC) gene family. Surprisingly, Pals1-depleted nephrons keep the SLC's segment-specific expression and subcellular distribution, demonstrating that the phenotype is not mainly due to dysfunctional apicobasal cell polarization of renal epithelia. Our data may provide first hints that SLCs may act as modulating factors for renal cyst formation.
    Language English
    Publishing date 2022-02-16
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2814330-9
    ISSN 2296-889X
    ISSN 2296-889X
    DOI 10.3389/fmolb.2022.792829
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Hypoxia hits APOL1 in the kidney.

    Grampp, Steffen / Krüger, René / Lauer, Victoria / Uebel, Sebastian / Knaup, Karl X / Naas, Julia / Höffken, Verena / Weide, Thomas / Schiffer, Mario / Naas, Stephanie / Schödel, Johannes

    Kidney international

    2023  Volume 104, Issue 1, Page(s) 53–60

    Abstract: Individuals of African ancestry carrying two pathogenic variants of apolipoprotein 1 (APOL1) have a substantially increased risk for developing chronic kidney disease. The course of APOL1 nephropathy is extremely heterogeneous and shaped by systemic ... ...

    Abstract Individuals of African ancestry carrying two pathogenic variants of apolipoprotein 1 (APOL1) have a substantially increased risk for developing chronic kidney disease. The course of APOL1 nephropathy is extremely heterogeneous and shaped by systemic factors such as a response to interferon. However, additional environmental factors operating in this second-hit model have been less well defined. Here, we reveal that stabilization of hypoxia-inducible transcription factors (HIF) by hypoxia or HIF prolyl hydroxylase inhibitors activates transcription of APOL1 in podocytes and tubular cells. An active regulatory DNA-element upstream of APOL1 that interacted with HIF was identified. This enhancer was accessible preferentially in kidney cells. Importantly, upregulation of APOL1 by HIF was additive to the effects of interferon. Furthermore, HIF stimulated expression of APOL1 in tubular cells derived from the urine of an individual carrying a risk variant for kidney disease. Thus, hypoxic insults may serve as important modulators of APOL1 nephropathy.
    MeSH term(s) Humans ; Apolipoprotein L1/genetics ; Genetic Predisposition to Disease ; Kidney/pathology ; Renal Insufficiency, Chronic/genetics ; Renal Insufficiency, Chronic/pathology ; Interferons ; Apolipoproteins/genetics
    Chemical Substances Apolipoprotein L1 ; Interferons (9008-11-1) ; Apolipoproteins ; APOL1 protein, human
    Language English
    Publishing date 2023-04-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 120573-0
    ISSN 1523-1755 ; 0085-2538
    ISSN (online) 1523-1755
    ISSN 0085-2538
    DOI 10.1016/j.kint.2023.03.035
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Early decrease of blood myeloid-derived suppressor cells during checkpoint inhibition is a favorable biomarker in metastatic melanoma.

    Gaißler, Andrea / Bochem, Jonas / Spreuer, Janine / Ottmann, Shannon / Martens, Alexander / Amaral, Teresa / Wagner, Nikolaus Benjamin / Claassen, Manfred / Meier, Friedegund / Terheyden, Patrick / Garbe, Claus / Eigentler, Thomas / Weide, Benjamin / Pawelec, Graham / Wistuba-Hamprecht, Kilian

    Journal for immunotherapy of cancer

    2023  Volume 11, Issue 6

    Abstract: Background: The need for reliable clinical biomarkers to predict which patients with melanoma will benefit from immune checkpoint blockade (ICB) remains unmet. Several different parameters have been considered in the past, including routine differential ...

    Abstract Background: The need for reliable clinical biomarkers to predict which patients with melanoma will benefit from immune checkpoint blockade (ICB) remains unmet. Several different parameters have been considered in the past, including routine differential blood counts, T cell subset distribution patterns and quantification of peripheral myeloid-derived suppressor cells (MDSC), but none has yet achieved sufficient accuracy for clinical utility.
    Methods: Here, we investigated potential cellular biomarkers from clinical routine blood counts as well as several myeloid and T cell subsets, using flow cytometry, in two independent cohorts of a total of 141 patients with stage IV M1c melanoma before and during ICB.
    Results: Elevated baseline frequencies of monocytic MDSCs (M-MDSC) in the blood were confirmed to predict shorter overall survival (OS) (HR 2.086, p=0.030) and progression-free survival (HR 2.425, p=0.001) in the whole patient cohort. However, we identified a subgroup of patients with highly elevated baseline M-MDSC frequencies that fell below a defined cut-off during therapy and found that these patients had a longer OS that was similar to that of patients with low baseline M-MDSC frequencies. Importantly, patients with high M-MDSC frequencies exhibited a skewed baseline distribution of certain other immune cells but these did not influence patient survival, illustrating the paramount utility of MDSC assessment.
    Conclusion: We confirmed that in general, highly elevated frequencies of peripheral M-MDSC are associated with poorer outcomes of ICB in metastatic melanoma. However, one reason for an imperfect correlation between high baseline MDSCs and outcome for individual patients may be the subgroup of patients identified here, with rapidly decreasing M-MDSCs on therapy, in whom the negative effect of high M-MDSC frequencies was lost. These findings might contribute to developing more reliable predictors of late-stage melanoma response to ICB at the individual patient level. A multifactorial model seeking such markers yielded only MDSC behavior and serum lactate dehydrogenase as predictors of treatment outcome.
    MeSH term(s) Humans ; Myeloid-Derived Suppressor Cells ; Melanoma/pathology ; Biomarkers ; Treatment Outcome ; Flow Cytometry
    Chemical Substances Biomarkers
    Language English
    Publishing date 2023-06-05
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2719863-7
    ISSN 2051-1426 ; 2051-1426
    ISSN (online) 2051-1426
    ISSN 2051-1426
    DOI 10.1136/jitc-2023-006802
    Database MEDical Literature Analysis and Retrieval System OnLINE

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