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  1. Article ; Online: Comparison of Pan-Lyssavirus RT-PCRs and Development of an Improved Protocol for Surveillance of Non-RABV Lyssaviruses.

    Drzewnioková, Petra / Marciano, Sabrina / Leopardi, Stefania / Panzarin, Valentina / De Benedictis, Paola

    Viruses

    2023  Volume 15, Issue 3

    Abstract: Rabies is a zoonotic and fatal encephalitis caused by members of ... ...

    Abstract Rabies is a zoonotic and fatal encephalitis caused by members of the
    MeSH term(s) Animals ; Humans ; Lyssavirus/genetics ; Rabies/diagnosis ; Rabies/veterinary ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Viral/genetics ; RNA, Viral/analysis ; Real-Time Polymerase Chain Reaction/methods ; Chiroptera ; Rhabdoviridae Infections/diagnosis ; Rhabdoviridae Infections/veterinary
    Chemical Substances RNA, Viral
    Language English
    Publishing date 2023-03-04
    Publishing country Switzerland
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v15030680
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Alternative Methods to Current In Vivo Procedures to Address the 3Rs Tenet in Rabies Proficiency Testing.

    Zorzan, Maira / Gourlaouen, Morgane / Leopardi, Stefania / De Benedictis, Paola

    Viruses

    2022  Volume 14, Issue 8

    Abstract: Canine rabies is responsible for an estimated 59,000 human deaths every year. In an attempt to reach the ZeroBy30 goal, robust disease surveillance coupled with improved diagnostics play a paramount role in ensuring reliable data and gradually attesting ... ...

    Abstract Canine rabies is responsible for an estimated 59,000 human deaths every year. In an attempt to reach the ZeroBy30 goal, robust disease surveillance coupled with improved diagnostics play a paramount role in ensuring reliable data and gradually attesting rabies control advancements. In this context, proficiency testing is organized to harmonize rabies diagnostic capacities. In most exercises, rabies-positive samples consist of brains collected from intracerebrally inoculated mice. This procedure causes distress and severe suffering to animals, raising important ethical concerns that can no longer be ignored. In the last decades, the 3Rs tenet (Replace, Reduce, Refine) has been successfully implemented in several scientific areas, and we strongly support its application in the framework of rabies proficiency testing. Here, we discuss cell-based technologies as innovative sustainable in vitro candidate systems to replace in vivo experiments for the production of proficiency testing samples. The application of these alternative methods can allow completely in vitro or ex vivo production of rabies proficiency testing panels, which would represent an important replacement or reduction/refinement for current in vivo procedures.
    MeSH term(s) Animals ; Dogs ; Humans ; Laboratory Proficiency Testing ; Mice ; Rabies/diagnosis ; Rabies Vaccines
    Chemical Substances Rabies Vaccines
    Language English
    Publishing date 2022-07-31
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14081698
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: The Importance of Accurate Host Species Identification in the Framework of Rabies Surveillance, Control and Elimination.

    De Benedictis, Paola / Leopardi, Stefania / Markotter, Wanda / Velasco-Villa, Andres

    Viruses

    2022  Volume 14, Issue 3

    Abstract: Accurate host identification is paramount to understand disease epidemiology and to apply appropriate control measures. This is especially important for multi-host pathogens such as the rabies virus, a major and almost invariably fatal zoonosis that has ... ...

    Abstract Accurate host identification is paramount to understand disease epidemiology and to apply appropriate control measures. This is especially important for multi-host pathogens such as the rabies virus, a major and almost invariably fatal zoonosis that has mobilized unanimous engagement at an international level towards the final goal of zero human deaths due to canine rabies. Currently, diagnostic laboratories implement a standardized identification using taxonomic keys. However, this method is challenged by high and undiscovered biodiversity, decomposition of carcasses and subjective misevaluation, as has been attested to by findings from a cohort of 242 archived specimens collected across Sub-Saharan Africa and submitted for rabies diagnosis. We applied two simple and cheap methods targeting the Cytochrome b and Cytochrome c oxidase subunit I to confirm the initial classification. We therefore suggest prioritizing a standardized protocol that includes, as a first step, the implementation of taxonomic keys at a family or subfamily level, followed by the molecular characterization of the host species.
    MeSH term(s) Africa South of the Sahara ; Animals ; Dog Diseases/diagnosis ; Dog Diseases/epidemiology ; Dog Diseases/prevention & control ; Dogs ; Humans ; Laboratories ; Rabies/epidemiology ; Rabies/prevention & control ; Rabies/veterinary ; Rabies virus ; Zoonoses/epidemiology ; Zoonoses/prevention & control
    Language English
    Publishing date 2022-02-28
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14030492
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Transmission dynamics of lyssavirus in

    Kim, Younjung / Leopardi, Stefania / Scaravelli, Dino / Zecchin, Barbara / Priori, Pamela / Festa, Francesca / Drzewnioková, Petra / De Benedictis, Paola / Nouvellet, Pierre

    Proceedings. Biological sciences

    2023  Volume 290, Issue 1997, Page(s) 20230183

    Abstract: We investigated the transmission dynamics of lyssavirus ... ...

    Abstract We investigated the transmission dynamics of lyssavirus in
    MeSH term(s) Humans ; Pregnancy ; Animals ; Female ; Rhabdoviridae Infections/epidemiology ; Rhabdoviridae Infections/veterinary ; Chiroptera ; Seroepidemiologic Studies ; Antibodies, Viral ; RNA, Viral/analysis
    Chemical Substances Antibodies, Viral ; RNA, Viral
    Language English
    Publishing date 2023-04-19
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 209242-6
    ISSN 1471-2954 ; 0080-4649 ; 0962-8452 ; 0950-1193
    ISSN (online) 1471-2954
    ISSN 0080-4649 ; 0962-8452 ; 0950-1193
    DOI 10.1098/rspb.2023.0183
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1364: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Silent circulation of coronaviruses in pigs.

    Leopardi, Stefania / Terregino, Calogero / Paola, De Benedictis

    The Veterinary record

    2020  Volume 186, Issue 10, Page(s) 323

    MeSH term(s) Animals ; Asymptomatic Infections/epidemiology ; Coronavirus/isolation & purification ; Coronavirus Infections/diagnosis ; Coronavirus Infections/epidemiology ; Coronavirus Infections/veterinary ; Italy/epidemiology ; Swine ; Swine Diseases/diagnosis ; Swine Diseases/epidemiology ; Swine Diseases/virology
    Keywords covid19
    Language English
    Publishing date 2020-03-09
    Publishing country England
    Document type Letter
    ZDB-ID 390015-0
    ISSN 2042-7670 ; 0042-4900
    ISSN (online) 2042-7670
    ISSN 0042-4900
    DOI 10.1136/vr.m932
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    In stock of ZB MED Cologne/Königswinter

    Zs.B 645: Show issues Location:
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  6. Article ; Online: Synanthropic and Wild Animals as Sentinels of Zoonotic Agents: A Study of

    Mazzotta, Elisa / Bellinati, Laura / Bertasio, Cristina / Boniotti, Maria Beatrice / Lucchese, Laura / Ceglie, Letizia / Martignago, Federico / Leopardi, Stefania / Natale, Alda

    International journal of environmental research and public health

    2023  Volume 20, Issue 5

    Abstract: Leptospirosis is an infectious disease widely reported in veterinary practice and a worldwide zoonosis. In Northeastern Italy, different serogroups and genotypes ... ...

    Abstract Leptospirosis is an infectious disease widely reported in veterinary practice and a worldwide zoonosis. In Northeastern Italy, different serogroups and genotypes of
    MeSH term(s) Animals ; Dogs ; Rats ; Leptospira/genetics ; Animals, Wild ; Multilocus Sequence Typing ; Foxes/genetics ; Hedgehogs/genetics ; Clonidine ; Leptospirosis/veterinary ; Genotype ; Italy ; Chiroptera/genetics
    Chemical Substances ((E)-N-4-(1,2,3,4-tetrahydroisoquinolin-2-yl)butyl)-3-phenylacrylamide ; Clonidine (MN3L5RMN02)
    Language English
    Publishing date 2023-02-21
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2175195-X
    ISSN 1660-4601 ; 1661-7827
    ISSN (online) 1660-4601
    ISSN 1661-7827
    DOI 10.3390/ijerph20053783
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Discovery of a coronavirus in the Eurasian badger (Meles meles) belonging to a putative new genus.

    Zamperin, Gianpiero / Festa, Francesca / Palumbo, Elisa / Quaranta, Erika / Monne, Isabella / Terregino, Calogero / De Benedictis, Paola / Leopardi, Stefania

    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases

    2023  Volume 109, Page(s) 105406

    Abstract: In the aftermath of COVID-19, coronaviruses gained renewed attention by the scientific community. The study reports the identification and genetic characterization of a novel coronavirus in the European badger (Meles meles) obtained in the framework of ... ...

    Abstract In the aftermath of COVID-19, coronaviruses gained renewed attention by the scientific community. The study reports the identification and genetic characterization of a novel coronavirus in the European badger (Meles meles) obtained in the framework of passive surveillance implemented in Italian wildlife in response to the pandemic. Positive samples were characterized using next generation sequencing as well as genetic and phylogenetic analyses, aiming for taxonomic placement under ICTV guidelines of the viruses contained in each sample. Results obtained for six conserved domains within the polyprotein showed that the virus clustered as outgroup and shared <46% amino acid identity with other coronaviruses, supporting the assumption that it belongs to a new putative genus Epsiloncoronavirus. This finding highlights that mammals still hide diverse coronaviruses whose zoonotic and epizootic potential remains unknown.
    MeSH term(s) Animals ; Mustelidae ; Phylogeny ; COVID-19 ; Animals, Wild
    Language English
    Publishing date 2023-02-09
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2037068-4
    ISSN 1567-7257 ; 1567-1348
    ISSN (online) 1567-7257
    ISSN 1567-1348
    DOI 10.1016/j.meegid.2023.105406
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5645: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Article ; Online: Discovery of a coronavirus in the Eurasian badger (Meles meles) belonging to a putative new genus

    Zamperin, Gianpiero / Festa, Francesca / Palumbo, Elisa / Quaranta, Erika / Monne, Isabella / Terregino, Calogero / De Benedictis, Paola / Leopardi, Stefania

    Infection, Genetics and Evolution. , p.105406-

    2023  , Page(s) 105406–

    Abstract: In the aftermath of COVID-19, coronaviruses gained renewed attention by the scientific community. The study reports the identification and genetic characterization of a novel coronavirus in the European badger (Meles meles) obtained in the framework of ... ...

    Abstract In the aftermath of COVID-19, coronaviruses gained renewed attention by the scientific community. The study reports the identification and genetic characterization of a novel coronavirus in the European badger (Meles meles) obtained in the framework of passive surveillance implemented in Italian wildlife in response to the pandemic. Positive samples were characterized using next generation sequencing as well as genetic and phylogenetic analyses, aiming for taxonomic placement under ICTV guidelines of the viruses contained in each sample. Results obtained for six conserved domains within the polyprotein showed that the virus clustered as outgroup and shared <46% amino acid identity with other coronaviruses, supporting the assumption that it belongs to a new putative genus Epsiloncoronavirus. This finding highlights that mammals still hide diverse coronaviruses whose zoonotic and epizootic potential remains unknown.
    Keywords COVID-19 infection ; Meles meles ; Orthocoronavirinae ; amino acids ; badgers ; epizootic diseases ; genetics ; infection ; monitoring ; new genus ; pandemic ; phylogeny ; polyproteins ; viruses ; wildlife ; Coronavirus ; Eurasian badger ; Mustelids ; Zoonoses
    Language English
    Publishing place Elsevier B.V.
    Document type Article ; Online
    Note Pre-press version ; Use and reproduction
    ZDB-ID 2037068-4
    ISSN 1567-1348
    ISSN 1567-1348
    DOI 10.1016/j.meegid.2023.105406
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5645: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  10. Article ; Online: Synanthropic and Wild Animals as Sentinels of Zoonotic Agents

    Elisa Mazzotta / Laura Bellinati / Cristina Bertasio / Maria Beatrice Boniotti / Laura Lucchese / Letizia Ceglie / Federico Martignago / Stefania Leopardi / Alda Natale

    International Journal of Environmental Research and Public Health, Vol 20, Iss 3783, p

    A Study of Leptospira Genotypes Circulating in Northeastern Italy

    2023  Volume 3783

    Abstract: Leptospirosis is an infectious disease widely reported in veterinary practice and a worldwide zoonosis. In Northeastern Italy, different serogroups and genotypes of Leptospira have been described in ill dogs, the most commonly detected being ... ...

    Abstract Leptospirosis is an infectious disease widely reported in veterinary practice and a worldwide zoonosis. In Northeastern Italy, different serogroups and genotypes of Leptospira have been described in ill dogs, the most commonly detected being Icterohaemorragiae (ICT) ST 17, Australis (AUS) ST 24 and ST 198, Pomona (POM) ST 117 and ST 289, and Sejroe (SEJ) ST 155. However, there is little information available on the environmental exposure to Leptospira of wild and synanthropic animals. The aim of this study was to identify the circulating genotypes in potential reservoirs to fill this gap of knowledge. Between 2015 and 2022, 681 animal carcasses collected by the Public Veterinary Service were analyzed for Leptospira with a real-time PCR-based screening test, while positive samples were genotyped by multi-locus sequence typing analysis. To carry out our study, we tested 330 hedgehogs, 105 red foxes, 108 Norway rats, 79 mice, 22 coypus, 10 bank voles, 13 grey wolves, 5 common shrews and 9 greater mouse-eared bats. Five sequence types (STs) common in dogs were also found in wild animals: ST 24, ST 198, ST 17 and ST 155 in hedgehogs, ST 17 and ST 24 in foxes, ST 17 in rats, ST 17 and ST 155 in mice, and ST 117 in a wolf. In addition, to the best of the authors’ knowledge, this is the first Italian report of SEJ ST 197 in a bank vole. Furthermore, this study described a previous survey conducted in 2009 on coypus (30 animals from the province of Trento and 41 from the province of Padua), referring to a serological positivity ( L . Bratislava) without any molecular detection of Leptospira . This study on Leptospira in synanthropic and wild animals highlighted the importance of increasing our epidemiological knowledge of leptospirosis and its zoonotic risks.
    Keywords Leptospira ; synanthropic animals ; environmental reservoirs ; multi-locus sequence typing ; real-time PCR ; Medicine ; R
    Subject code 630
    Language English
    Publishing date 2023-02-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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