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Article: Evaluation of cytotoxicity, oxidative stress and organ-specific effects of activated carbon from Al-Baha date palm kernels.

Alothaid, Hani

2022 Volume 29, Issue 9, Page(s) 103387

Abstract: Background: Activated carbon (AC) is a carbonaceous material derived from carbonization and activation of carbon-containing compounds at high temperature and has a large surface area, providing it with excellent adsorption properties. Human exposure to ... ...

Abstract	Background: Activated carbon (AC) is a carbonaceous material derived from carbonization and activation of carbon-containing compounds at high temperature and has a large surface area, providing it with excellent adsorption properties. Human exposure to ACs via ingestion is increasing and, unfortunately, there is little to no evidence related to its level of toxicity. Materials and methods: Activated carbon of powdered date kernels from Al-Baha city in Saudi Arabia were used to treat rats and cell lines (HepG2 and HCT-116). Toxicity, microbiological tests and biochemical analyses were carried out to investigate biological activity of both commercially available AC (CAC), pharmaceutical AC (PAC) and AC from date palm kernels (AAC). Results: None of the ACs showed activity on Conclusion: AAC appears to be biologically safe compared with PAC and CAC due to its antioxidant activities and non-effect on both hepatic and renal functions.
Language	English
Publishing date	2022-07-22
Publishing country	Saudi Arabia
Document type	Journal Article
ZDB-ID	2515206-3
ISSN	2213-7106 ; 1319-562X
ISSN (online)	2213-7106
ISSN	1319-562X
DOI	10.1016/j.sjbs.2022.103387
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Article ; Online: The Development and Optimization of Lipid-Based Self-Nanoemulsifying Drug Delivery Systems for the Intravenous Delivery of Propofol.

Kazi, Mohsin / Alqahtani, Athba / Alharbi, Majed / Ahmad, Ajaz / Hussain, Muhammad Delwar / Alothaid, Hani / Aldughaim, Mohammed S

Molecules (Basel, Switzerland)

2023 Volume 28, Issue 3

Abstract: Purpose: Propofol is a relatively short-acting potent anesthetic lipophilic drug used during short surgical procedures. Despite the success of propofol intravenous emulsions, drawbacks to such formulations include inherent emulsion instability, the lack ...

Abstract	Purpose: Propofol is a relatively short-acting potent anesthetic lipophilic drug used during short surgical procedures. Despite the success of propofol intravenous emulsions, drawbacks to such formulations include inherent emulsion instability, the lack of a safe vehicle to prevent sepsis, and concern regarding hyperlipidemia-related side effects. The aim of the current investigation was to develop a novel, lipid-based self-nanoemulsifying drug delivery system (SNEDDS) for propofol with improved stability and anesthetic activity for human use. Methods: A series of SNEDDS formulations were developed using naturally obtained medium-chain/long-chain mono-, di-, and triglycerides, glyceryl monocaprylate, and water-soluble cosolvents with hydrogenated castor oil constructing ternary phase diagrams for propofol. The developed SNEDDS formulations were characterized using visual observation, particle size analysis, zeta potential, transmission electron microscopy, equilibrium solubility, in vitro dynamic dispersion and stability, and in vivo sleeping disorder studies in rats. The in vivo bioavailability of the SNEDDSs in rats was also studied to compare the representative formulations with the marketed product Diprivan Results: Medium-chain triglycerides (M810) with mono-diglycerides (CMCM) as an oil blend and hydrogenated castor oil (KHS15) as a surfactant were selected as key ingredients in ternary phase diagram studies. The nanoemulsifying regions were identified from the studies and a number of SNEDDSs were formulated. Results from the characterization studies demonstrated the formation of efficient nanosized particles (28-45 nm globule size, 0.10-0.20 PDI) in the optimized SNEDDS with a drug loading of 50 mg/g, which is almost 500-fold higher than free propofol. TEM analysis showed the formation of spherical and homogeneous nanoparticles of less than 50 nm. The dissolution rate of the representative SNEDDS was faster than raw propofol and able to maintain 99% propofol in aqueous solution for around 24 h. The optimized liquid SNEDDS formulation was found to be thermodynamically stable. The intravenous administration of the SNEDDS in male Wistar rats induced a sleeping time of 73-88 min. The mean plasma concentrations after the IV administration of propofol nano-formulations PF2-SNEDDS and PF8-SNEDDS were 1348.07 ± 27.31 and 1138.66 ± 44.97 µg/mL, as compared to 891.44 ± 26.05 µg/mL ( Conclusion: Propofol-loaded SNEDDS formulations could be a potential pharmaceutical product with improved stability, bioavailability, and anesthetic activity.
MeSH term(s)	Rats ; Male ; Humans ; Animals ; Propofol ; Rats, Wistar ; Castor Oil ; Drug Delivery Systems/methods ; Solubility ; Emulsions ; Nanoparticles ; Biological Availability ; Triglycerides ; Administration, Intravenous ; Particle Size ; Administration, Oral ; Drug Liberation
Chemical Substances	Propofol (YI7VU623SF) ; Castor Oil (8001-79-4) ; Emulsions ; Triglycerides
Language	English
Publishing date	2023-02-03
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules28031492
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Article: Effectiveness of Early Favipiravir Therapy in Hospitalised COVID-19 Patients.

Tawfik, Abdulrahman / Alzahrani, Abdulrahman / Alharbi, Sami / Almitairi, Jamal / Alzahrani, Arwa / Alshehri, Mohammed Ali / Aldughaim, Mohammed S / Alothaid, Hani

Advances in virology

2022 Volume 2022, Page(s) 9240941

Abstract: COVID-19 is a disease caused by a novel coronavirus with no specific, standard treatment. We investigated the clinical data of COVID-19 patients admitted to King Fahad Specialist Hospital (KFSH) in Buraydah by comparing the patients who were treated ... ...

Abstract	COVID-19 is a disease caused by a novel coronavirus with no specific, standard treatment. We investigated the clinical data of COVID-19 patients admitted to King Fahad Specialist Hospital (KFSH) in Buraydah by comparing the patients who were treated early with favipiravir (within 3 days of admission) to patients who were treated after three days of admission or not treated. 165 patients were confirmed with PCR tests and admitted to KFSH for treatment. Comorbidities contributed significantly to increasing the length of stay in hospital at 11.4 ± 0.8 days compared to patients with no comorbidities at 8.6 ± 0.9 days (
Language	English
Publishing date	2022-06-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	2625776-2
ISSN	1687-8647 ; 1687-8639
ISSN (online)	1687-8647
ISSN	1687-8639
DOI	10.1155/2022/9240941
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Article ; Online: The Development and Optimization of Lipid-Based Self-Nanoemulsifying Drug Delivery Systems for the Intravenous Delivery of Propofol

Mohsin Kazi / Athba Alqahtani / Majed Alharbi / Ajaz Ahmad / Muhammad Delwar Hussain / Hani Alothaid / Mohammed S. Aldughaim

Molecules, Vol 28, Iss 1492, p

2023 Volume 1492

Abstract	Purpose: Propofol is a relatively short-acting potent anesthetic lipophilic drug used during short surgical procedures. Despite the success of propofol intravenous emulsions, drawbacks to such formulations include inherent emulsion instability, the lack of a safe vehicle to prevent sepsis, and concern regarding hyperlipidemia-related side effects. The aim of the current investigation was to develop a novel, lipid-based self-nanoemulsifying drug delivery system (SNEDDS) for propofol with improved stability and anesthetic activity for human use. Methods: A series of SNEDDS formulations were developed using naturally obtained medium-chain/long-chain mono-, di-, and triglycerides, glyceryl monocaprylate, and water-soluble cosolvents with hydrogenated castor oil constructing ternary phase diagrams for propofol. The developed SNEDDS formulations were characterized using visual observation, particle size analysis, zeta potential, transmission electron microscopy, equilibrium solubility, in vitro dynamic dispersion and stability, and in vivo sleeping disorder studies in rats. The in vivo bioavailability of the SNEDDSs in rats was also studied to compare the representative formulations with the marketed product Diprivan ® . Results: Medium-chain triglycerides (M810) with mono-diglycerides (CMCM) as an oil blend and hydrogenated castor oil (KHS15) as a surfactant were selected as key ingredients in ternary phase diagram studies. The nanoemulsifying regions were identified from the studies and a number of SNEDDSs were formulated. Results from the characterization studies demonstrated the formation of efficient nanosized particles (28–45 nm globule size, 0.10–0.20 PDI) in the optimized SNEDDS with a drug loading of 50 mg/g, which is almost 500-fold higher than free propofol. TEM analysis showed the formation of spherical and homogeneous nanoparticles of less than 50 nm. The dissolution rate of the representative SNEDDS was faster than raw propofol and able to maintain 99% propofol in aqueous solution for around 24 h. The ...
Keywords	self-nanoemulsifying drug delivery systems ; propofol ; pharmacokinetics ; sleeping disorder ; intravenous delivery ; Organic chemistry ; QD241-441
Subject code	660
Language	English
Publishing date	2023-02-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Role of calcineurin biosignaling in cell secretion and the possible regulatory mechanisms.

Alothaid, Hani / Aldughaim, Mohammed S K / Alamri, Saad Saeed / Alrahimi, Jehan Saad M / Al-Jadani, Saeedah Hamdan

Saudi journal of biological sciences

2020 Volume 28, Issue 1, Page(s) 116–124

Abstract: Cyclic adenosine monophosphate (cAMP) and calcium ions ( ... ...

Abstract	Cyclic adenosine monophosphate (cAMP) and calcium ions (Ca
Language	English
Publishing date	2020-09-03
Publishing country	Saudi Arabia
Document type	Journal Article ; Review
ZDB-ID	2515206-3
ISSN	2213-7106 ; 1319-562X
ISSN (online)	2213-7106
ISSN	1319-562X
DOI	10.1016/j.sjbs.2020.08.042
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Article ; Online: Association between interleukin-27 gene polymorphisms and

Aljarba, Nada H / Al-Anazi, Mashael R / Al-Hazani, Tahani M / Shafeai, Mohammed I / Rudiny, Fuad H / Motaen, Ali M / Bin Dajem, Saad M / Alothaid, Hani / Alghamdi, Jahad / Alkahtani, Saad / Al-Qahtani, Ahmed A

Innate immunity

2023 Volume 29, Issue 5, Page(s) 83–94

Abstract: Malaria is often characterized by a complicated disease course due to multifaceted intrinsic genetic factors of the host and the parasite. This study aimed to investigate the role of interleukin-27 ( ...

Abstract	Malaria is often characterized by a complicated disease course due to multifaceted intrinsic genetic factors of the host and the parasite. This study aimed to investigate the role of interleukin-27 (
MeSH term(s)	Humans ; Infant ; Child, Preschool ; Interleukin-27/genetics ; Plasmodium falciparum/genetics ; Parasitemia/genetics ; Parasitemia/epidemiology ; Case-Control Studies ; Saudi Arabia ; Malaria, Falciparum/genetics ; Malaria, Falciparum/epidemiology ; Malaria, Falciparum/parasitology ; Malaria ; Polymorphism, Genetic
Chemical Substances	Interleukin-27
Language	English
Publishing date	2023-06-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2381250-3
ISSN	1753-4267 ; 1753-4259
ISSN (online)	1753-4267
ISSN	1753-4259
DOI	10.1177/17534259231178594
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Article ; Online: Unraveling activity of crucial domain HABD protein in dengue virus.

Patel, Ayyub / El-Gamal, Basiouny / Abd Ellatif, Mohamed / Alothaid, Hani / Mirdad, Tarek M / Almalki, Waleed Hassan / Alwabli, Afaf S

Cellular and molecular biology (Noisy-le-Grand, France)

2022 Volume 68, Issue 4, Page(s) 66–74

Abstract: Dengue virus (DENV) causes dengue, which is a very common mosquito-borne viral disease. The global incidence of dengue has increased dramatically in recent decades. About half of the world's population is now at risk. This virus is widespread throughout ... ...

Abstract	Dengue virus (DENV) causes dengue, which is a very common mosquito-borne viral disease. The global incidence of dengue has increased dramatically in recent decades. About half of the world's population is now at risk. This virus is widespread throughout the tropics, which are influenced by rainfall, temperature, and humidity; however, severe dengue has a higher risk of death when not managed timely. To describe Dengue virus helicase ATP binding domain (HABD) protein in biochemically characterized. Sequences analysis, structure modeling, secondary structure prediction, ATPase assay, unwinding assay, RNA binding assay. HABD has RNA-dependent ATPase and helicase activity which are crucial proteins that participate in the unwinding of double-stranded DNA or RNA by utilizing ATP. RNA binding proteins and DEAD-box RNA helicases have been revealed to contribute to viral replication. Moreover, DEAD-box RNA helicases have been demonstrated to be involved in several features of cellular metabolism of RNA, for example, transcription, splicing, biogenesis, ribosomal processing of RNA, etc. In the present study, we have mainly focused on the Dengue virus's helicase ATP binding domain (HABD) and observed that HABD contains RNA-dependent ATPase and unwinding activity at different concentrations and time points.
MeSH term(s)	Adenosine Triphosphatases/metabolism ; Adenosine Triphosphate/metabolism ; Animals ; Carrier Proteins/metabolism ; DEAD-box RNA Helicases/chemistry ; DEAD-box RNA Helicases/metabolism ; Dengue/genetics ; Dengue Virus/genetics ; Dengue Virus/metabolism ; RNA/metabolism
Chemical Substances	Carrier Proteins ; RNA (63231-63-0) ; Adenosine Triphosphate (8L70Q75FXE) ; Adenosine Triphosphatases (EC 3.6.1.-) ; DEAD-box RNA Helicases (EC 3.6.4.13)
Language	English
Publishing date	2022-04-30
Publishing country	France
Document type	Journal Article
ZDB-ID	1161779-2
ISSN	1165-158X ; 0145-5680
ISSN (online)	1165-158X
ISSN	0145-5680
DOI	10.14715/cmb/2022.68.4.9
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Article: In vivo

Hadad, Sahar El / Alsolami, Maha / Aldahlawi, Alia / Alrahimi, Jehan / Basingab, Fatemah / Hassoubah, Shahira / Alothaid, Hani

Saudi journal of biological sciences

2021 Volume 28, Issue 8, Page(s) 4751–4761

Abstract: Probiotics have attracted considerable attention because of their ability to ameliorate disease and prevent cancer. In this study, we examined the immunomodulatory effects of ... ...

Abstract	Probiotics have attracted considerable attention because of their ability to ameliorate disease and prevent cancer. In this study, we examined the immunomodulatory effects of a
Language	English
Publishing date	2021-05-06
Publishing country	Saudi Arabia
Document type	Journal Article
ZDB-ID	2515206-3
ISSN	2213-7106 ; 1319-562X
ISSN (online)	2213-7106
ISSN	1319-562X
DOI	10.1016/j.sjbs.2021.04.090
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Article ; Online: SARS-CoV-2 modulates inflammatory responses of alveolar epithelial type II cells

Al-Qahtani, Ahmed A / Pantazi, Ioanna / Alhamlan, Fatimah S / Alothaid, Hani / Matou-Nasri, Sabine / Sourvinos, George / Vergadi, Eleni / Tsatsanis, Christos

Frontiers in immunology

2022 Volume 13, Page(s) 1020624

Abstract: Background: SARS-CoV-2 infects through the respiratory route and triggers inflammatory response by affecting multiple cell types including type II alveolar epithelial cells. SARS-CoV-2 triggers signals : Aim: Aim of the present study was to ... ...

Abstract	Background: SARS-CoV-2 infects through the respiratory route and triggers inflammatory response by affecting multiple cell types including type II alveolar epithelial cells. SARS-CoV-2 triggers signals Aim: Aim of the present study was to investigate the effect of SARS-CoV2 on type II alveolar epithelial cells, focusing on signals initiated by its S protein and their impact on the expression of inflammatory mediators. Results: For this purpose A549 alveolar type II epithelial cells were exposed to SARS CoV2 S recombinant protein and the expression of inflammatory mediators was measured. The results showed that SARS-CoV-2 S protein decreased the expression and secretion of IL8, IL6 and TNFα, 6 hours following stimulation, while it had no effect on IFNα, CXCL5 and PAI-1 expression. We further examined whether SARS-CoV-2 S protein, when combined with TLR2 signals, which are also triggered by SARS-CoV2 and its envelope protein, exerts a different effect in type II alveolar epithelial cells. Simultaneous treatment of A549 cells with SARS-CoV-2 S protein and the TLR2 ligand PAM3csk4 decreased secretion of IL8, IL6 and TNFα, while it significantly increased IFNα, CXCL5 and PAI-1 mRNA expression. To investigate the molecular pathway through which SARS-CoV-2 S protein exerted this immunomodulatory action in alveolar epithelial cells, we measured the induction of MAPK/ERK and PI3K/AKT pathways and found that SARS-CoV-2 S protein induced the activation of the serine threonine kinase AKT. Treatment with the Akt inhibitor MK-2206, abolished the inhibitory effect of SARS-CoV-2 S protein on IL8, IL6 and TNFα expression, suggesting that SARS-CoV-2 S protein mediated its action Conclusion: The findings of our study, showed that SARS-CoV-2 S protein suppressed inflammatory responses in alveolar epithelial type II cells at early stages of infection through activation of the PI3K/AKT pathway. Thus, our results suggest that at early stages SARS-CoV-2 S protein signals inhibit immune responses to the virus allowing it to propagate the infection while in combination with TLR2 signals enhances PAI-1 expression, potentially affecting the local coagulation cascade.
MeSH term(s)	Humans ; Alveolar Epithelial Cells/metabolism ; SARS-CoV-2 ; Phosphatidylinositol 3-Kinases/metabolism ; Proto-Oncogene Proteins c-akt ; Tumor Necrosis Factor-alpha ; RNA, Viral ; Plasminogen Activator Inhibitor 1 ; Interleukin-6 ; Interleukin-8 ; Toll-Like Receptor 2 ; COVID-19
Chemical Substances	spike protein, SARS-CoV-2 ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Tumor Necrosis Factor-alpha ; RNA, Viral ; Plasminogen Activator Inhibitor 1 ; Interleukin-6 ; Interleukin-8 ; Toll-Like Receptor 2
Language	English
Publishing date	2022-10-31
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.1020624
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Article: Exposure to Cadmium Telluride Quantum Dots and Gene Expression Profile of Huh-7 Hepatocellular Carcinoma Cell Line.

Alothaid, Hani / Al-Anazi, Mashael R / Al-Qahtani, Arwa A / Colak, Dilek / Yusuf, Azeez / Aldughaim, Mohammed S / Mahzari, Ali M / Habibullah, Mahmoud M / Alarifi, Saud / Alkahtani, Saad / Al-Qahtani, Ahmed A

Dose-response : a publication of International Hormesis Society

2023 Volume 21, Issue 3, Page(s) 15593258231185457

Abstract: Nanoparticles have shown promising potential for efficient drug delivery, circumventing biological interferences like immunological and renal clearance and mechanical and enzymatic destruction. However, a handful of research papers have questioned the ... ...

Abstract	Nanoparticles have shown promising potential for efficient drug delivery, circumventing biological interferences like immunological and renal clearance and mechanical and enzymatic destruction. However, a handful of research papers have questioned the biomedical use of metal-based nanoparticles like cadmium telluride quantum dots (CdTe-QDs) for their cytotoxic, genotoxic, and carcinogenic potential. Herein, we examined the effects of CdTe-QD NPs on gene expression profile of hepatocellular carcinoma (Huh-7) cell line. Huh-7 cells were treated with CdTe-QD NPs (10 μg/ml for 6, 12, and 24 hours, and 25 μg/ml for 6 and 12 hours), and transcriptomic analysis was performed using microarray to evaluate the global gene expression profile. Differential expressed genes (DEGs) were observed for both the doses (10 and 25 μg/ml) of CdTe-QD NPs at different time points. Gene ontology (GO) analysis revealed that genes involved in molecular function of cell cycle, organizational injury and abnormalities, cell death and survival, gene expression, cancer, organismal survival, and cellular development were differentially expressed. Overall, we have demonstrated differential expression of several genes, involved in maintaining cell survival, metabolism, and genome integrity. These findings were confirmed by RT-qPCR study for some canonical pathway genes signifying possible implication in NP toxicity-mediated cell survival and inhibition of cell death.
Language	English
Publishing date	2023-08-24
Publishing country	United States
Document type	Journal Article
ZDB-ID	2440820-7
ISSN	1559-3258
ISSN	1559-3258
DOI	10.1177/15593258231185457
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