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  1. Article ; Online: Generation, Characterization, and Count of Fluorescent Extracellular Vesicles.

    Ferrantelli, Flavia / Tirelli, Valentina / Barreca, Valeria / Manfredi, Francesco

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2504, Page(s) 207–217

    Abstract: Extracellular vesicles (EVs) are membranous particles released by all cells in the external milieu. Depending on their origin, they are given different names: exosomes are nanovesicles that originate from the endosomal compartment, whereas microvesicles ... ...

    Abstract Extracellular vesicles (EVs) are membranous particles released by all cells in the external milieu. Depending on their origin, they are given different names: exosomes are nanovesicles that originate from the endosomal compartment, whereas microvesicles bud from plasma membrane. Both contain molecules that are crucial for the onset and spreading of different pathologies, from neurodegenerative diseases to cancer, and are considered promising disease markers. On the other hand, EVs are often used as therapeutic tools, and can be engineered to carry drugs and chemicals. This chapter describes a method to produce EVs, mainly exosomes, containing the green fluorescent protein (GFP) linked to an exosome anchoring protein (Nef
    MeSH term(s) Cell-Derived Microparticles/metabolism ; Exosomes/metabolism ; Extracellular Vesicles/metabolism ; Flow Cytometry
    Language English
    Publishing date 2022-04-25
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2341-1_15
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Activation of Anti-SARS-CoV-2 Human CTLs by Extracellular Vesicles Engineered with the N Viral Protein.

    Manfredi, Francesco / Chiozzini, Chiara / Ferrantelli, Flavia / Leone, Patrizia / Giovannelli, Andrea / Sanchez, Massimo / Federico, Maurizio

    Vaccines

    2022  Volume 10, Issue 7

    Abstract: We propose an innovative anti-SARS-CoV-2 immune strategy based on extracellular vesicles (EVs) inducing an anti-SARS-CoV-2 N ... ...

    Abstract We propose an innovative anti-SARS-CoV-2 immune strategy based on extracellular vesicles (EVs) inducing an anti-SARS-CoV-2 N CD8
    Language English
    Publishing date 2022-06-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines10071060
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Long-Term Antitumor CD8

    Ferrantelli, Flavia / Manfredi, Francesco / Chiozzini, Chiara / Leone, Patrizia / Giovannelli, Andrea / Olivetta, Eleonora / Federico, Maurizio

    Cancers

    2021  Volume 13, Issue 9

    Abstract: We developed an innovative method to induce antigen-specific ... ...

    Abstract We developed an innovative method to induce antigen-specific CD8
    Language English
    Publishing date 2021-05-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers13092263
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: The C-Terminal Domain of Nef

    Chiozzini, Chiara / Manfredi, Francesco / Ferrantelli, Flavia / Leone, Patrizia / Giovannelli, Andrea / Olivetta, Eleonora / Federico, Maurizio

    Vaccines

    2021  Volume 9, Issue 4

    Abstract: Intramuscular injection of DNA vectors expressing the extracellular vesicle (EV)-anchoring protein ... ...

    Abstract Intramuscular injection of DNA vectors expressing the extracellular vesicle (EV)-anchoring protein Nef
    Language English
    Publishing date 2021-04-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines9040373
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Simultaneous CD8

    Ferrantelli, Flavia / Chiozzini, Chiara / Manfredi, Francesco / Giovannelli, Andrea / Leone, Patrizia / Federico, Maurizio

    Vaccines

    2021  Volume 9, Issue 3

    Abstract: Most advanced vaccines against severe acute respiratory syndrome coronavirus (SARS-CoV)-2 are designed to induce antibodies against spike (S) protein. Differently, we developed an original strategy to induce ... ...

    Abstract Most advanced vaccines against severe acute respiratory syndrome coronavirus (SARS-CoV)-2 are designed to induce antibodies against spike (S) protein. Differently, we developed an original strategy to induce CD8
    Language English
    Publishing date 2021-03-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines9030240
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: N-Terminal Fatty Acids of NEF

    Chiozzini, Chiara / Manfredi, Francesco / Arenaccio, Claudia / Ferrantelli, Flavia / Leone, Patrizia / Federico, Maurizio

    Vaccines

    2020  Volume 8, Issue 2

    Abstract: We recently described a cytotoxic ... ...

    Abstract We recently described a cytotoxic CD8
    Language English
    Publishing date 2020-05-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines8020243
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Engineered Extracellular Vesicles/Exosomes as a New Tool against Neurodegenerative Diseases.

    Ferrantelli, Flavia / Chiozzini, Chiara / Leone, Patrizia / Manfredi, Francesco / Federico, Maurizio

    Pharmaceutics

    2020  Volume 12, Issue 6

    Abstract: Neurodegenerative diseases are commonly generated by intracellular accumulation of misfolded/aggregated mutated proteins. These abnormal protein aggregates impair the functions of mitochondria and induce oxidative stress, thereby resulting in neuronal ... ...

    Abstract Neurodegenerative diseases are commonly generated by intracellular accumulation of misfolded/aggregated mutated proteins. These abnormal protein aggregates impair the functions of mitochondria and induce oxidative stress, thereby resulting in neuronal cell death. In turn, neuronal damage induces chronic inflammation and neurodegeneration. Thus, reducing/eliminating these abnormal protein aggregates is a priority for any anti-neurodegenerative therapeutic approach. Although several antibodies against mutated neuronal proteins have been already developed, how to efficiently deliver them inside the target cells remains an unmet issue. Extracellular vesicles/exosomes incorporating intrabodies against the pathogenic products would be a tool for innovative therapeutic approaches. In this review/perspective article, we identify and describe the major molecular targets associated with neurodegenerative diseases, as well as the antibodies already developed against them. Finally, we propose a novel targeting strategy based on the endogenous engineering of extracellular vesicles/exosomes constitutively released by cells of the central nervous system.
    Language English
    Publishing date 2020-06-09
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2527217-2
    ISSN 1999-4923
    ISSN 1999-4923
    DOI 10.3390/pharmaceutics12060529
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Activation of Anti-SARS-CoV-2 Human CTLs by Extracellular Vesicles Engineered with the N Viral Protein

    Francesco Manfredi / Chiara Chiozzini / Flavia Ferrantelli / Patrizia Leone / Andrea Giovannelli / Massimo Sanchez / Maurizio Federico

    Vaccines, Vol 10, Iss 1060, p

    2022  Volume 1060

    Abstract: We propose an innovative anti-SARS-CoV-2 immune strategy based on extracellular vesicles (EVs) inducing an anti-SARS-CoV-2 N CD8 + T cytotoxic lymphocyte (CTL) immune response. We previously reported that the SARS-CoV-2 N protein can be uploaded at high ... ...

    Abstract We propose an innovative anti-SARS-CoV-2 immune strategy based on extracellular vesicles (EVs) inducing an anti-SARS-CoV-2 N CD8 + T cytotoxic lymphocyte (CTL) immune response. We previously reported that the SARS-CoV-2 N protein can be uploaded at high levels in EVs upon fusion with Nef mut , i.e., a biologically inactive HIV-1 Nef mutant incorporating into EVs at quite high levels. Here, we analyze the immunogenic properties in human cells of EVs engineered with SARS-CoV-2 N fused at the C-terminus of either Nef mut or a deletion mutant of Nef mut referred to as Nef mut PL. The analysis of in vitro-produced EVs has supported the uploading of N protein when fused with truncated Nef mut . Mice injected with DNA vectors expressed each fusion protein developed robust SARS-CoV-2 N-specific CD8 + T cell immune responses. When ex vivo human dendritic cells were challenged with EVs engineered with either fusion products, the induction of a robust N-specific CTL activity, as evaluated by both CD107a and trogocytosis assays, was observed. Through these data we achieved the proof-of-principle that engineered EVs can be instrumental to elicit anti-SARS-CoV-2 CTL immune response in human cells. This achievement represents a mandatory step towards the upcoming experimentations in pre-clinical models focused on intranasal administration of N-engineered EVs.
    Keywords extracellular vesicles ; SARS-CoV-2 ; CTL immunity ; HIV-1 Nef ; CD107a ; Medicine ; R
    Language English
    Publishing date 2022-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article: SARS-CoV-2-Specific CD8

    Ferrantelli, Flavia / Manfredi, Francesco / Chiozzini, Chiara / Leone, Patrizia / Pugliese, Katherina / Spada, Massimo / Di Virgilio, Antonio / Giovannelli, Andrea / Valeri, Mauro / Cara, Andrea / Michelini, Zuleika / Andreotti, Mauro / Federico, Maurizio

    Vaccines

    2023  Volume 11, Issue 9

    Abstract: Severe acute respiratory syndrome coronavirus (SARS-CoV)-2 enters the host by infecting nasal ciliated cells. Then, the virus can spread towards the oropharyngeal cavity and the pulmonary tissues. The antiviral adaptive immunity is promptly induced in ... ...

    Abstract Severe acute respiratory syndrome coronavirus (SARS-CoV)-2 enters the host by infecting nasal ciliated cells. Then, the virus can spread towards the oropharyngeal cavity and the pulmonary tissues. The antiviral adaptive immunity is promptly induced in response to the virus's detection, with virus-specific T-lymphocytes appearing before antiviral antibodies. Both the breadth and potency of antiviral CD8
    Language English
    Publishing date 2023-08-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines11091433
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Antiviral effect of SARS-CoV-2 N-specific CD8

    Manfredi, Francesco / Chiozzini, Chiara / Ferrantelli, Flavia / Leone, Patrizia / Pugliese, Katherina / Spada, Massimo / Di Virgilio, Antonio / Giovannelli, Andrea / Valeri, Mauro / Cara, Andrea / Michelini, Zuleika / Andreotti, Mauro / Federico, Maurizio

    NPJ vaccines

    2023  Volume 8, Issue 1, Page(s) 83

    Abstract: Induction of effective immunity in the lungs should be a requisite for any vaccine designed to control the severe pathogenic effects generated by respiratory infectious agents. We recently provided evidence that the generation of endogenous extracellular ...

    Abstract Induction of effective immunity in the lungs should be a requisite for any vaccine designed to control the severe pathogenic effects generated by respiratory infectious agents. We recently provided evidence that the generation of endogenous extracellular vesicles (EVs) engineered for the incorporation of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV)-2 Nucleocapsid (N) protein induced immunity in the lungs of K18-hACE2 transgenic mice, which then can survive the lethal virus infection. However, nothing is known about the ability of the N-specific CD8
    Language English
    Publishing date 2023-06-02
    Publishing country England
    Document type Journal Article
    ISSN 2059-0105
    ISSN (online) 2059-0105
    DOI 10.1038/s41541-023-00686-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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