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Article ; Online: Duplicated Flagellins in Pseudomonas Divergently Contribute to Motility and Plant Immune Elicitation.

Luo, Yuan / Wang, Jing / Gu, Yi-Lin / Zhang, Li-Qun / Wei, Hai-Lei

2023 Volume 11, Issue 1, Page(s) e0362122

Abstract: Flagellins are the main constituents of the flagellar filaments that provide bacterial motility, chemotactic ability, and host immune elicitation ability. Although the functions of flagellins have been extensively studied in bacteria with a single ... ...

Abstract	Flagellins are the main constituents of the flagellar filaments that provide bacterial motility, chemotactic ability, and host immune elicitation ability. Although the functions of flagellins have been extensively studied in bacteria with a single flagellin-encoding gene, the function of multiple flagellin-encoding genes in a single bacterial species is largely unknown. Here, the model plant-growth-promoting bacterium Pseudomonas kilonensis F113 was used to decipher the divergent functions of duplicated flagellins. We demonstrate that the two flagellins (FliC-1 and FliC-2) in 12 Pseudomonas strains, including F113, are evolutionarily distinct. Only the
MeSH term(s)	Flagellin/genetics ; Flagellin/metabolism ; Pseudomonas/genetics ; Pseudomonas/metabolism ; Plant Immunity
Chemical Substances	Flagellin (12777-81-0)
Language	English
Publishing date	2023-01-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.03621-22
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Article: [Inhibitory Effect of PKC412 Against Human Acute Leukemia Cell Line HL-60 Cells].

Yu, Li-Qun / Liu, Jian-Qiong / Gu, Xue-Zhong

Zhongguo shi yan xue ye xue za zhi

2020 Volume 29, Issue 1, Page(s) 62–67

Abstract: Objective: To explore the effects and mechanisms of PKC412 inhibitor on proliferation and apoptosis of HL-60 cell line.: Methods: CCK-8 assay was used to detect the effect of PKC412 on the proliferation of HL-60 cells at different concentrations; ... ...

Abstract	Objective: To explore the effects and mechanisms of PKC412 inhibitor on proliferation and apoptosis of HL-60 cell line. Methods: CCK-8 assay was used to detect the effect of PKC412 on the proliferation of HL-60 cells at different concentrations; Wright-Giemsa staining was used to estimated the effect of PKC412 on the apoptosis of HL-60 cells; the mRNA expression of BCL-2 and P53 genes was detected by qRT-PCR, the expression of BCL-2 and P53 proteins was detected by Western blot. HL-60 cells were injected into mouse caudal vein to construct acute myeloid leukemia model, PKC412 was administered to tail vein for 31.25 nmol/kg, normal saline was injected into the same site of the mice as control group, and the inhibitory effect of PKC412 on HL-60 cells in mice was observed. ELISA assay was used to detect the effect of PKC412 on the inflammatory factors of TNF-α and TGF-β in tumor mice. Results: PKC412 could inhibit the proliferation of HL-60 cell, which was in a dose dependent manner(r=0.9973) (IC50 was 0.31 μmol/L), and induce apoptosis of HL-60 cells. After HL-60 cell was treated by PKC412 for 48 h the expression of BCL-2 gene was down regulated(0.417±0.044 vs 0.933±0.033, t=9.347, P<0.001), the expression of P53 gene was up regulated(1.533±0.145 vs 1.050±0.161, t=2.231, P>0.05) as compared with control group. And the expression of BCL-2 protein was decreased, while the expression of P53 protein was increased. PKC412 could inhibited the growth of HL-60 tumor cells in vivo, the survival rate of mice after administration was 50% and the weight was increased as compared with that in control group(18.02±0.403 g vs 16.44±0.562 g, t=2.272, P=0.0356). The secretion of TNF-α and TGF-β cytokine in serum and spleen cells in PKC412 group was significantly lower than that in control group (P<0.05). Conclusion: PKC412 can induce apoptosis of HL-60 cells by inhibiting the expression level of BCL-2 gene, PKC412 administration in vivo can inhibit the growth of the tumors.
MeSH term(s)	Animals ; Apoptosis ; Cell Proliferation ; HL-60 Cells ; Humans ; Leukemia, Myeloid, Acute ; Mice ; Proto-Oncogene Proteins c-bcl-2 ; Staurosporine/analogs & derivatives
Chemical Substances	Proto-Oncogene Proteins c-bcl-2 ; Staurosporine (H88EPA0A3N) ; midostaurin (ID912S5VON)
Language	Chinese
Publishing date	2020-10-14
Publishing country	China
Document type	Journal Article
ZDB-ID	2404306-0
ISSN	1009-2137
ISSN	1009-2137
DOI	10.19746/j.cnki.issn.1009-2137.2021.01.010
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Zs.A 6603: Show issues

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Article ; Online: Engineering Novel (

Jia, Dong-Xu / Wang, Fan / Zhao, Rui / Gu, Bo-Di / Peng, Chen / Jin, Li-Qun / Liu, Zhi-Qiang / Zheng, Yu-Guo

Applied and environmental microbiology

2022 Volume 88, Issue 9, Page(s) e0006222

Abstract: d-Alanine belongs to nonessential amino acids that have diverse applications in the fields of food and health care. ( ...

Abstract	d-Alanine belongs to nonessential amino acids that have diverse applications in the fields of food and health care. (
MeSH term(s)	Alanine/metabolism ; Amino Acids ; Ascomycota ; Catalytic Domain ; Pyruvic Acid/metabolism ; Transaminases/metabolism
Chemical Substances	Amino Acids ; Pyruvic Acid (8558G7RUTR) ; Transaminases (EC 2.6.1.-) ; Alanine (OF5P57N2ZX)
Language	English
Publishing date	2022-04-25
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	223011-2
ISSN	1098-5336 ; 0099-2240
ISSN (online)	1098-5336
ISSN	0099-2240
DOI	10.1128/aem.00062-22
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Zs.B 201: Show issues

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Article ; Online: Unveiling the biointerfaces characteristics and removal pathways of Cr(Ⅵ) in Bacillus cereus FNXJ1-2-3 for the Cr(Ⅵ)-to-Cr(0) conversion.

Li, Zhaoxia / Cui, Entian / Gu, Naipeng / Ma, Weixing / Guo, Qingyuan / Li, Xuan / Jin, Jianxiang / Wang, Qun / Ding, Cheng

Environmental research

2024 Volume 251, Issue Pt 2, Page(s) 118663

Abstract: Although less toxic than hexavalent chromium, Cr (Ⅲ) species still pose a threat to human health. The Cr (Ⅵ) should be converted to Cr (0) instead of Cr (Ⅲ), which is still involved in biological detoxification filed. Herein, for the first time, it was ... ...

Abstract	Although less toxic than hexavalent chromium, Cr (Ⅲ) species still pose a threat to human health. The Cr (Ⅵ) should be converted to Cr (0) instead of Cr (Ⅲ), which is still involved in biological detoxification filed. Herein, for the first time, it was found that Cr(Ⅵ) can be reduced into Cr(0) by Bacillus cereus FNXJ1-2-3, a way to completely harmless treatment of Cr(Ⅵ). The bacterial strain exhibited excellent performance in the reduction, sorption, and accumulation of Cr(Ⅵ) and Cr (Ⅲ). XPS etching characterization inferred that the transformation of Cr(Ⅵ) into Cr(0) followed a reduction pathway of Cr(Ⅵ)→Cr (Ⅲ)→metallic Cr(0), in which at least two secretory chromium reductases (E
Language	English
Publishing date	2024-03-08
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	205699-9
ISSN	1096-0953 ; 0013-9351
ISSN (online)	1096-0953
ISSN	0013-9351
DOI	10.1016/j.envres.2024.118663
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Zs.A 726: Show issues

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Article ; Online: The aerolysin nanopore: from peptidomic to genomic applications.

Wang, Yong / Gu, Li-Qun / Tian, Kai

Nanoscale

2018 Volume 10, Issue 29, Page(s) 13857–13866

Abstract: The aerolysin pore (ARP) is a newly emerging nanopore that has been extensively used for peptide and protein sensing. Recently, several groups have explored the application of ARP in detecting genetic and epigenetic markers. This brief review summarizes ... ...

Abstract	The aerolysin pore (ARP) is a newly emerging nanopore that has been extensively used for peptide and protein sensing. Recently, several groups have explored the application of ARP in detecting genetic and epigenetic markers. This brief review summarizes the current applications of ARP, progressing from peptidomic to genomic detection; the recently reported site-directed mutagenesis of ARP; and new genomic DNA sensing approaches, and their advantages and disadvantages. This review will also discuss the perspectives and future applications of ARP for nucleic acid sequencing and biomolecule sensing.
MeSH term(s)	Bacterial Toxins/chemistry ; Genomics ; Mutagenesis, Site-Directed ; Nanopores ; Oligonucleotides/analysis ; Pore Forming Cytotoxic Proteins/chemistry ; Protein Structure, Tertiary
Chemical Substances	Bacterial Toxins ; Oligonucleotides ; Pore Forming Cytotoxic Proteins ; aerolysin (53126-24-2)
Language	English
Publishing date	2018-07-12
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	2515664-0
ISSN	2040-3372 ; 2040-3364
ISSN (online)	2040-3372
ISSN	2040-3364
DOI	10.1039/c8nr04255a
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Article ; Online: Selective covalent capture of a DNA sequence corresponding to a cancer-driving C>G mutation in the

Guo, Xu / Nejad, Maryam Imani / Gu, Li-Qun / Gates, Kent S

RSC advances

2019 Volume 9, Issue 56, Page(s) 32804–32810

Abstract: Covalent reactions are used in the detection of various biological analytes ranging from low molecular weight metabolites to protein-protein complexes. The detection of specific nucleic acid sequences is important in molecular biology and medicine but ... ...

Abstract	Covalent reactions are used in the detection of various biological analytes ranging from low molecular weight metabolites to protein-protein complexes. The detection of specific nucleic acid sequences is important in molecular biology and medicine but covalent approaches are less common in this field, in part, due to a deficit of simple and reliable reactions for the covalent capture of target sequences. Covalent anchoring can prevent the denaturation (melting) of probe-target complexes and causes signal degradation in typical hybridization-based assays. Here, we used chemically reactive nucleic acid probes that hybridize with, and covalently capture, a target sequence corresponding to a cancer-driving variant of the human
Language	English
Publishing date	2019-10-15
Publishing country	England
Document type	Journal Article
ISSN	2046-2069
ISSN (online)	2046-2069
DOI	10.1039/c9ra08009k
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Article ; Online: High-Throughput, Quantitative Screening of Quorum-Sensing Inhibitors Based on a Bacterial Biosensor.

Zhang, Jun-Wei / Guo, Cong / Xuan, Chen-Guang / Gu, Jing-Wen / Cui, Zi-Ning / Zhang, Jing / Zhang, Lixin / Jiang, Wenjun / Zhang, Li-Qun

ACS chemical biology

2023 Volume 18, Issue 12, Page(s) 2544–2554

Abstract: Quorum sensing (QS) is a cell-cell communication mechanism by which bacteria synchronize social behaviors such as biofilm formation and virulence factor secretion by producing and sensing small molecular signals. Quorum quenching (QQ) by degrading ... ...

Abstract	Quorum sensing (QS) is a cell-cell communication mechanism by which bacteria synchronize social behaviors such as biofilm formation and virulence factor secretion by producing and sensing small molecular signals. Quorum quenching (QQ) by degrading signals or blocking signal transmissions has become a promising strategy for disrupting QS and preventing bacterial infection and biofilm formation. However, studies of high-throughput screening and identification approaches for quorum-sensing inhibitors (QSIs) are still inadequate. In this work, we developed a sensitive, high-throughput approach for screening QSIs based on the bacterial biosensor strain
MeSH term(s)	Quorum Sensing ; Bacteria/metabolism ; Virulence Factors/metabolism ; Biosensing Techniques ; High-Throughput Screening Assays
Chemical Substances	Virulence Factors
Language	English
Publishing date	2023-11-20
Publishing country	United States
Document type	Journal Article
ISSN	1554-8937
ISSN (online)	1554-8937
DOI	10.1021/acschembio.3c00537
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Article ; Online: Biomedical diagnosis perspective of epigenetic detections using alpha-hemolysin nanopore.

Wang, Yong / Gu, Li-Qun

AIMS materials science

2015 Volume 2, Issue 4, Page(s) 448–472

Abstract: The α-hemolysin nanopore has been studied for applications in DNA sequencing, various single-molecule detections, biomolecular interactions, and biochips. The detection of single molecules in a clinical setting could dramatically improve cancer detection ...

Abstract	The α-hemolysin nanopore has been studied for applications in DNA sequencing, various single-molecule detections, biomolecular interactions, and biochips. The detection of single molecules in a clinical setting could dramatically improve cancer detection and diagnosis as well as develop personalized medicine practices for patients. This brief review shortly presents the current solid state and protein nanopore platforms and their applications like biosensing and sequencing. We then elaborate on various epigenetic detections (like microRNA, G-quadruplex, DNA damages, DNA modifications) with the most widely used alpha-hemolysin pore from a biomedical diagnosis perspective. In these detections, a nanopore electrical current signature was generated by the interaction of a target with the pore. The signature often was evidenced by the difference in the event duration, current level, or both of them. An ideal signature would provide obvious differences in the nanopore signals between the target and the background molecules. The development of cancer biomarker detection techniques and nanopore devices have the potential to advance clinical research and resolve health problems. However, several challenges arise in applying nanopore devices to clinical studies, including super low physiological concentrations of biomarkers resulting in low sensitivity, complex biological sample contents resulting in false signals, and fast translocating speed through the pore resulting in poor detections. These issues and possible solutions are discussed.
Language	English
Publishing date	2015-11-16
Publishing country	United States
Document type	Journal Article
ZDB-ID	2777112-X
ISSN	2372-0484 ; 2372-0484
ISSN (online)	2372-0484
ISSN	2372-0484
DOI	10.3934/matersci.2015.4.448
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Article ; Online: Real-time label-free detection of dynamic aptamer-small molecule interactions using a nanopore nucleic acid conformational sensor.

Chingarande, Rugare G / Tian, Kai / Kuang, Yu / Sarangee, Aby / Hou, Chengrui / Ma, Emily / Ren, Jarett / Hawkins, Sam / Kim, Joshua / Adelstein, Ray / Chen, Sally / Gillis, Kevin D / Gu, Li-Qun

Proceedings of the National Academy of Sciences of the United States of America

2023 Volume 120, Issue 24, Page(s) e2108118120

Abstract: Nucleic acids can undergo conformational changes upon binding small molecules. These conformational changes can be exploited to develop new therapeutic strategies through control of gene expression or triggering of cellular responses and can also be used ...

Abstract	Nucleic acids can undergo conformational changes upon binding small molecules. These conformational changes can be exploited to develop new therapeutic strategies through control of gene expression or triggering of cellular responses and can also be used to develop sensors for small molecules such as neurotransmitters. Many analytical approaches can detect dynamic conformational change of nucleic acids, but they need labeling, are expensive, and have limited time resolution. The nanopore approach can provide a conformational snapshot for each nucleic acid molecule detected, but has not been reported to detect dynamic nucleic acid conformational change in response to small -molecule binding. Here we demonstrate a modular, label-free, nucleic acid-docked nanopore capable of revealing time-resolved, small molecule-induced, single nucleic acid molecule conformational transitions with millisecond resolution. By using the dopamine-, serotonin-, and theophylline-binding aptamers as testbeds, we found that these nucleic acids scaffolds can be noncovalently docked inside the MspA protein pore by a cluster of site-specific charged residues. This docking mechanism enables the ion current through the pore to characteristically vary as the aptamer undergoes conformational changes, resulting in a sequence of current fluctuations that report binding and release of single ligand molecules from the aptamer. This nanopore tool can quantify specific ligands such as neurotransmitters, elucidate nucleic acid-ligand interactions, and pinpoint the nucleic acid motifs for ligand binding, showing the potential for small molecule biosensing, drug discovery assayed via RNA and DNA conformational changes, and the design of artificial riboswitch effectors in synthetic biology.
MeSH term(s)	Nanopores ; Ligands ; Nucleic Acid Conformation ; RNA ; Riboswitch ; Aptamers, Nucleotide/chemistry
Chemical Substances	Ligands ; RNA (63231-63-0) ; Riboswitch ; Aptamers, Nucleotide
Language	English
Publishing date	2023-06-05
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	209104-5
ISSN	1091-6490 ; 0027-8424
ISSN (online)	1091-6490
ISSN	0027-8424
DOI	10.1073/pnas.2108118120
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Zs.A 1148: Show issues

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Article ; Online: Engineered Superhydrophilic/Superaerophobic Catalyst: Two-Dimensional Co(OH)

Li, Ting / Gu, Fei / Chen, Xiao Hui / Zhang, Qing / Fu, Hong Chuan / Luo, Hong Qun / Li, Nian Bing

Inorganic chemistry

2023 Volume 62, Issue 6, Page(s) 2784–2792

Abstract: Efficient electrocatalysts require not only a tunable electronic structure but also great active site accessibility and favorable mass transfer. Here, a two-dimensional/three-dimensional (2D/3D) hierarchical electrocatalyst consisting of Co(OH) ...

Abstract	Efficient electrocatalysts require not only a tunable electronic structure but also great active site accessibility and favorable mass transfer. Here, a two-dimensional/three-dimensional (2D/3D) hierarchical electrocatalyst consisting of Co(OH)
Language	English
Publishing date	2023-01-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	1484438-2
ISSN	1520-510X ; 0020-1669
ISSN (online)	1520-510X
ISSN	0020-1669
DOI	10.1021/acs.inorgchem.2c03910
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