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  1. Article ; Online: Zhenqing recipe alleviates diabetic nephropathy in experimental type 2 diabetic rats through suppression of SREBP-1c.

    Wen, Xiuying / Zeng, Yali / Liu, Lifang / Zhang, Hui / Xu, Wenguang / Li, Ningxu / Jia, Xuming

    Journal of ethnopharmacology

    2012  Volume 142, Issue 1, Page(s) 144–150

    Abstract: Ethnopharmacological relevance: Zhenqing Recipe (ZQR), a Chinese herbal prescription, is used ...

    Abstract Ethnopharmacological relevance: Zhenqing Recipe (ZQR), a Chinese herbal prescription, is used to improve renal function of patients with diabetic nephropathy. In current research, the therapeutic effects of ZQR on type 2 diabetic nephropathy and the underlying molecular mechanisms were explored.
    Materials and methods: Animal model with diabetic nephropathy was developed by high fat/sucrose diet feeding plus streptozotocin injection for 4 weeks. The diabetic rats were then orally administered with ZQR extract at the dose of 4 g/kg, 8 g/kg body weight/day for 8 weeks.
    Results: Serum glucose, triglyceride and total cholesterol in untreated diabetic rats were significantly higher than that of normal control rats. ZQR treatment not only reduced serum glucose level in diabetic rats, but also decreased serum triglyceride and total cholesterol in a dose-dependent manner. Urinary albumin excretion rate, serum urea and creatinine were significantly decreased in ZQR groups compared with untreated diabetic group. Histopathological study of kidney samples showed that extracellular mesangial matrix expansion in diabetic rats was suppressed by ZQR treatment. Both mRNA and protein levels of sterol regulatory element binding-protein-1c (SREBP-1c), and its target genes including acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS) in renal cortex were significantly decreased in ZQR treated rats compared to untreated diabetic rats. Consequently, renal triglyceride was significantly reduced in ZQR groups. Furthermore, ZQR significantly inhibited the overexpression of transforming growth factor-β1 and fibronectin in the renal cortex of diabetic rats.
    Conclusions: Oral treatment of ZQR improved diabetic nephropathy by inhibiting the overexpression of SREBP-1c and its target genes including ACC and FAS in experimental type 2 diabetic rats.
    MeSH term(s) Acetyl-CoA Carboxylase/genetics ; Animals ; Diabetes Mellitus, Experimental/drug therapy ; Diabetes Mellitus, Experimental/pathology ; Diabetes Mellitus, Experimental/physiopathology ; Diabetes Mellitus, Type 2/drug therapy ; Diabetes Mellitus, Type 2/pathology ; Diabetes Mellitus, Type 2/physiopathology ; Diabetic Nephropathies/drug therapy ; Diabetic Nephropathies/pathology ; Diabetic Nephropathies/physiopathology ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Fatty Acid Synthases/genetics ; Fibronectins/metabolism ; Male ; Phytotherapy ; Protective Agents/pharmacology ; Protective Agents/therapeutic use ; RNA, Messenger/metabolism ; Rats ; Rats, Wistar ; Sterol Regulatory Element Binding Protein 1/genetics ; Transforming Growth Factor beta1/metabolism
    Chemical Substances Drugs, Chinese Herbal ; Fibronectins ; Protective Agents ; RNA, Messenger ; Sterol Regulatory Element Binding Protein 1 ; Transforming Growth Factor beta1 ; zhenqing ; Fatty Acid Synthases (EC 2.3.1.85) ; Acetyl-CoA Carboxylase (EC 6.4.1.2)
    Language English
    Publishing date 2012-06-26
    Publishing country Ireland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2012.04.028
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Zhenqing recipe alleviates diabetic nephropathy in experimental type 2 diabetic rats through suppression of SREBP-1c

    Wen, Xiuying / Zeng, Yali / Liu, Lifang / Zhang, Hui / Xu, Wenguang / Li, Ningxu / Jia, Xuming

    Journal of ethnopharmacology. 2012 June 26, v. 142, no. 1

    2012  

    Abstract: ETHNOPHARMACOLOGICAL RELEVANCE: Zhenqing Recipe (ZQR), a Chinese herbal prescription, is used ...

    Abstract ETHNOPHARMACOLOGICAL RELEVANCE: Zhenqing Recipe (ZQR), a Chinese herbal prescription, is used to improve renal function of patients with diabetic nephropathy. In current research, the therapeutic effects of ZQR on type 2 diabetic nephropathy and the underlying molecular mechanisms were explored. MATERIALS AND METHODS: Animal model with diabetic nephropathy was developed by high fat/sucrose diet feeding plus streptozotocin injection for 4 weeks. The diabetic rats were then orally administered with ZQR extract at the dose of 4g/kg, 8g/kg body weight/day for 8 weeks. RESULTS: Serum glucose, triglyceride and total cholesterol in untreated diabetic rats were significantly higher than that of normal control rats. ZQR treatment not only reduced serum glucose level in diabetic rats, but also decreased serum triglyceride and total cholesterol in a dose-dependent manner. Urinary albumin excretion rate, serum urea and creatinine were significantly decreased in ZQR groups compared with untreated diabetic group. Histopathological study of kidney samples showed that extracellular mesangial matrix expansion in diabetic rats was suppressed by ZQR treatment. Both mRNA and protein levels of sterol regulatory element binding-protein-1c (SREBP-1c), and its target genes including acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS) in renal cortex were significantly decreased in ZQR treated rats compared to untreated diabetic rats. Consequently, renal triglyceride was significantly reduced in ZQR groups. Furthermore, ZQR significantly inhibited the overexpression of transforming growth factor-β1 and fibronectin in the renal cortex of diabetic rats. CONCLUSIONS: Oral treatment of ZQR improved diabetic nephropathy by inhibiting the overexpression of SREBP-1c and its target genes including ACC and FAS in experimental type 2 diabetic rats.
    Keywords acetyl-CoA carboxylase ; albumins ; animal models ; blood glucose ; body weight ; cholesterol ; cortex ; creatinine ; diabetic nephropathy ; diet ; excretion ; extracellular matrix ; fatty-acid synthase ; gene targeting ; histopathology ; intravenous injection ; kidneys ; messenger RNA ; oral administration ; patients ; rats ; recipes ; renal function ; streptozotocin ; sucrose ; triacylglycerols ; urea
    Language English
    Dates of publication 2012-0626
    Size p. 144-150.
    Publishing place Elsevier Ireland Ltd
    Document type Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2012.04.028
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Applying Blockchain Technology in Network Public Opinion Risk Management System in Big Data Environment.

    Luo, Zhenqing / Zhang, Cheng

    publication RETRACTED

    Computational intelligence and neuroscience

    2023  Volume 2023, Page(s) 5212712

    Abstract: Network public opinion represents public social opinion to a certain extent and has an important impact on formulating national policies and judgment. Therefore, China and other countries attach great importance to the study of online public opinion. ... ...

    Abstract Network public opinion represents public social opinion to a certain extent and has an important impact on formulating national policies and judgment. Therefore, China and other countries attach great importance to the study of online public opinion. However, the current researches lack the combination of theory and practical cases and lack the intersection of social and natural sciences. This work aims to overcome the technical defects of traditional management systems, break through the difficulties and pain points of existing network public opinion risk management, and improve the efficiency of network public opinion risk management. Firstly, a network public opinion isolation strategy based on the infectious disease propagation model is proposed, and the optimal control theory is used to realize a functional control model to maximize social utility. Secondly, blockchain technology is used to build a network public opinion risk management system. The system is used to conduct a detailed study on identifying and perceiving online public opinion risk. Finally, a Chinese word segmentation scheme based on Long Short-Term Memory (LSTM) network model and a text emotion recognition scheme based on a convolutional neural network are proposed. Both schemes are validated on a typical corpus. The results show that when the system has a control strategy, the number of susceptible drops significantly. Two days after the public opinion is generated, the number of susceptible people decreased from 1,000 to 250; 3 days after the public opinion is generated, the number of susceptible people stabilized. 2 days after the public opinion is generated, the number of lurkers increased from 100 to 620; 3 days after the public opinion is generated, the number of lurkers stabilized. The data demonstrate that the designed isolation control strategy is effective. Changes in public opinion among infected people show that quarantine control strategies played a significant role in the early days of Corona Virus Disease 2019. The rate of change in the number of infections is more affected when quarantine controls are increased, especially in the days leading up to the outbreak. When the system adopts the optimal control strategy, the influence scope of public opinion becomes smaller, and the control becomes easier. When the dimension of the word vector of emergent events is 200, its accuracy may be higher. This method provides certain ideas for blockchain and deep learning technology in network public opinion control.
    MeSH term(s) Humans ; Public Opinion ; Big Data ; COVID-19 ; Blockchain ; Technology
    Language English
    Publishing date 2023-03-10
    Publishing country United States
    Document type Journal Article ; Retracted Publication
    ZDB-ID 2388208-6
    ISSN 1687-5273 ; 1687-5273
    ISSN (online) 1687-5273
    ISSN 1687-5273
    DOI 10.1155/2023/5212712
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Influence of drought intensity on soil carbon priming and its temperature sensitivity after rewetting.

    Zhang, Rui / Li, Rong / Kuang, Jialiang / Shi, Zhenqing

    The Science of the total environment

    2023  Volume 908, Page(s) 168362

    Abstract: Global climate change can affect the soil thermal and moisture condition, potentially disrupting microbial-mediated soil respiration and altering the soil C cycle. However, the complex relationship between the soil C turnover and transient thermal and ... ...

    Abstract Global climate change can affect the soil thermal and moisture condition, potentially disrupting microbial-mediated soil respiration and altering the soil C cycle. However, the complex relationship between the soil C turnover and transient thermal and moisture conditions is not fully understood. Specifically, quantitative understanding is lacking regarding the impact of drought-rewetting events and temperature on the response of soil organic carbon (SOC) decomposition rate to exogenous C input, known as the priming effects (PEs). Herein, we quantified glucose-induced PEs during the rewetting of soils incubated under two drought intensities [with 20 % and 33 % water holding capacity (WHC)], at different incubation temperatures (15, 25, and 35 °C). Moreover, the effect size of drought intensities on PEs and the temperature sensitivity of PEs were quantified using lnRR (Response Ratio) and Q
    Language English
    Publishing date 2023-11-07
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2023.168362
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Effect of core layer parameters on the mechanical properties of biomimetic foamed silicone rubber sandwich structures against low-velocity impact.

    Zhang, Di / Dong, Hui / Guo, Debao / Wang, Zhenqing

    Journal of the mechanical behavior of biomedical materials

    2023  Volume 143, Page(s) 105931

    Abstract: In this paper, a red-eared slider turtle is used as a prototype for the bionic design of the foamed silicone rubber sandwich structure, and the effect of core layer parameters on the low-velocity impact resistance of the foamed silicone rubber sandwich ... ...

    Abstract In this paper, a red-eared slider turtle is used as a prototype for the bionic design of the foamed silicone rubber sandwich structure, and the effect of core layer parameters on the low-velocity impact resistance of the foamed silicone rubber sandwich structure is studied by the finite element method. A numerical model with porosity of the foamed silicone rubber intrinsic model and a three-dimensional Hashin fiber plate damage model were used to verify the validity of the model in comparison with the test. On this basis, finite element simulations were performed by varying the core layer density and thickness. The results show that from the perspective of energy absorption, the sandwich structure has better impact resistance with core density of 750 kg/m
    MeSH term(s) Silicone Elastomers ; Biomimetics ; Porosity ; Bionics
    Chemical Substances Silicone Elastomers
    Language English
    Publishing date 2023-05-23
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2378381-3
    ISSN 1878-0180 ; 1751-6161
    ISSN (online) 1878-0180
    ISSN 1751-6161
    DOI 10.1016/j.jmbbm.2023.105931
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: [Advances in heparin structural analysis by chromatography technologies].

    Ouyang, Yilan / Yi, Lin / Qiu, Luyun / Zhang, Zhenqing

    Se pu = Chinese journal of chromatography

    2023  Volume 41, Issue 2, Page(s) 107–121

    Abstract: Heparin (Hp) is the most widely used anticoagulant drug in the clinics, with an annual global output of over 10 billion dollars. Hp, a member of the glycosaminoglycans (GAGs), is prepared from porcine intestinal mucosa via extraction, separation, and ... ...

    Abstract Heparin (Hp) is the most widely used anticoagulant drug in the clinics, with an annual global output of over 10 billion dollars. Hp, a member of the glycosaminoglycans (GAGs), is prepared from porcine intestinal mucosa via extraction, separation, and purification. Hp is a linear polysaccharide with repeating disaccharide units. Low-molecular-weight heparins (LMWHs) are depolymerized from Hp via chemical or enzymatic degradation. Compared with Hp, LMWHs exhibit less bleeding side effect, milder immunogenicity, and higher bioavailability when injected subcutaneously. In general, Hps, including LMWHs, are high complex drugs with large molecular weights (MWs), inhomogeneous MW distributions, and structural heterogeneity, including different degrees and locations of sulfonation, and unique residues generated from different production processes. Thus, developing efficient analytical methods to elucidate the structures of Hps and characterize or quantitate their properties is extremely challenging. Unfortunately, this problem limits their quality control, production optimization, clinical safety monitoring, and new applications. Research has constantly sought to elucidate the complicated structures of Hp drugs. Among the structural analysis and quality control methods of Hp currently available, chromatographic methods are the most widely studied and used. However, no literature thoroughly summarizes the specific applications of chromatographic methods in the structural analysis, manufacturing process, and quality control of Hp drugs. This paper systematically organizes and describes recent research progresses of the chromatographic methods used to analyze Hp drugs, including the identification and composition of monosaccharides, disaccharides, oligosaccharides, and polysaccharides. The applications, innovations, and limitations of these chromatographic methods are also summarized in this review. The insights obtained in this study will help production and quality control personnel, as well as drug researchers, obtain a deeper understanding of the complex structures of Hp drugs. This paper also provides a comprehensive reference for the structural analysis and quality control of Hps, proposes ideas for the development of new quality control methods, and lays a strong foundation for the in-depth structural elucidation of Hp drugs.
    MeSH term(s) Animals ; Swine ; Heparin ; Heparin, Low-Molecular-Weight ; Anticoagulants ; Chromatography ; Heparin Lyase/metabolism ; Disaccharides
    Chemical Substances Heparin (9005-49-6) ; Heparin, Low-Molecular-Weight ; Anticoagulants ; Heparin Lyase (EC 4.2.2.7) ; Disaccharides
    Language Chinese
    Publishing date 2023-02-01
    Publishing country China
    Document type Review ; English Abstract ; Journal Article
    ISSN 1872-2059
    ISSN (online) 1872-2059
    DOI 10.3724/SP.J.1123.2022.07020
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Direct count of fluorescent microspheres in a microfluidic chip based on the capillary electrophoresis method.

    Yang, Jing / Li, Zhenqing / Zhang, Dawei / Yamaguchi, Yoshinori / Xiao, Wen

    Analytical methods : advancing methods and applications

    2023  Volume 15, Issue 25, Page(s) 3014–3018

    Abstract: Fluorescent microspheres (FMs) are tiny particles with special functions that are widely employed in biological research. Counting of microscale FMs is a great challenge by capillary electrophoresis. Herein we developed a method to count 2 μm FMs based ... ...

    Abstract Fluorescent microspheres (FMs) are tiny particles with special functions that are widely employed in biological research. Counting of microscale FMs is a great challenge by capillary electrophoresis. Herein we developed a method to count 2 μm FMs based on a microfluidic chip with a gradual change in inner size. Such a microfluidic chip can inhibit sample blocking at the inlet of the capillary. The results showed that FMs migrated in the wide part of the microchannel side by side, and then passed through the narrow part one by one. There was a linear relationship between the number of peaks in the electropherogram and concentration of FMs if they were running in the microchannel for more than 20 min. A high separation voltage may lead to aggregation of FMs in the microchannels, and about 2 × 10
    MeSH term(s) Microfluidics ; Microspheres ; Microfluidic Analytical Techniques/methods ; Electrophoresis, Capillary/methods
    Language English
    Publishing date 2023-06-29
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2515210-5
    ISSN 1759-9679 ; 1759-9660
    ISSN (online) 1759-9679
    ISSN 1759-9660
    DOI 10.1039/d3ay00710c
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Corrigendum to 'Negative effects of soil warming, and adaptive cultivation strategies of maize: A review' [Science of the Total Environment 862 (2023) 160738].

    Zhang, Shibo / Bai, Jingxuan / Zhang, Guixin / Xia, Zhenqing / Wu, Mengke / Lu, Haidong

    The Science of the total environment

    2023  Volume 893, Page(s) 164617

    Language English
    Publishing date 2023-06-16
    Publishing country Netherlands
    Document type Published Erratum
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2023.164617
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: RN0D, a galactoglucan from Panax notoginseng flower induces cancer cell death via PINK1/Parkin mitophagy.

    Ji, Meng / Sun, Long / Zhang, Minghui / Liu, Yulin / Zhang, Zhenqing / Wang, Peipei

    Carbohydrate polymers

    2024  Volume 332, Page(s) 121889

    Abstract: Metabolic alterations within mitochondria, encompassing processes such as autophagy and energy metabolism, play a pivotal role in facilitating the swift proliferation, invasion, and metastasis of cancer cells. Despite this, there is a scarcity of ... ...

    Abstract Metabolic alterations within mitochondria, encompassing processes such as autophagy and energy metabolism, play a pivotal role in facilitating the swift proliferation, invasion, and metastasis of cancer cells. Despite this, there is a scarcity of currently available medications with proven anticancer efficacy through the modulation of mitochondrial dysfunction in a clinical setting. Here, we introduce the structural characteristics of RN0D, a galactoglucan isolated and purified from Panax notoginseng flowers, mainly composed of β-1,4-galactan and β-1,3/1,6-glucan. RN0D demonstrates the capacity to induce mitochondrial impairment in cancer cells, leading to the accumulation of reactive oxygen species, initiation of mitophagy, and reduction in both mitochondrial number and size. This sequence of events ultimately results in the inhibition of mitochondrial and glycolytic bioenergetics, culminating in the demise of cancer cells due to adenosine triphosphate (ATP) deprivation. Notably, the observed bioactivity is attributed to RN0D's direct targeting of Galectin-3, as affirmed by surface plasmon resonance studies. Furthermore, RN0D is identified as an activator of the PTEN-induced kinase 1 (PINK1)/Parkin pathway, ultimately instigating cytotoxic mitophagy in tumor cells. This comprehensive study substantiates the rationale for advancing RN0D as a potentially efficacious anticancer therapeutic.
    MeSH term(s) Humans ; Mitophagy ; Panax notoginseng ; Galactans ; Glucans ; Cell Death ; Neoplasms ; Ubiquitin-Protein Ligases/metabolism ; Protein Kinases/metabolism ; Polysaccharides, Bacterial
    Chemical Substances galactoglucan (55787-15-0) ; Galactans ; Glucans ; Ubiquitin-Protein Ligases (EC 2.3.2.27) ; Protein Kinases (EC 2.7.-) ; Polysaccharides, Bacterial
    Language English
    Publishing date 2024-02-02
    Publishing country England
    Document type Journal Article
    ZDB-ID 1501516-6
    ISSN 1879-1344 ; 0144-8617
    ISSN (online) 1879-1344
    ISSN 0144-8617
    DOI 10.1016/j.carbpol.2024.121889
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: A Study on the Properties of Composite Modified Mortar with Styrene-Butadiene Rubber Latex and Silica Fume.

    Yan, Renwei / Wang, Laifa / Ni, Yongjun / Zhang, Shuowen / He, Zhenqing / Guan, Bowen

    Polymers

    2024  Volume 16, Issue 5

    Abstract: To solve the problem of the poor abrasion resistance of concrete pavement surface mortar, this study substituted cement with equal amounts of styrene-butadiene rubber (SBR) latex and silica fume (SF) to investigate the effects of organic/inorganic ... ...

    Abstract To solve the problem of the poor abrasion resistance of concrete pavement surface mortar, this study substituted cement with equal amounts of styrene-butadiene rubber (SBR) latex and silica fume (SF) to investigate the effects of organic/inorganic material composite modification on the fluidity, drying shrinkage, mechanical properties, and abrasion resistance of cement mortar. Also in this study, the microstructure, product, and pore structure characteristics of the composite modified cement mortar were investigated using scanning electron microscope (SEM), X-Ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), and the Brunauer-Emmett-Teller (BET) method. This research found that the sole substitution of SF negatively impacted the mortar's fluidity and drying shrinkage yet enhanced its mechanical strength and abrasion resistance; the incorporation of SBR latex improved fluidity, reduced shrinkage, and increased flexural strength but adversely affected the compressive strength of the mortar. Additionally, the enhancement of the mortar's abrasion resistance with SBR latex was significantly greater than that with SF. When SBR latex and SF were used together as substitutes, the latex struggled to offset the negative impact of SF on mortar fluidity but effectively reduced shrinkage; SF compensated for the detrimental effect of the latex on compressive strength. Moreover, the primary role in enhancing the mortar's abrasion resistance was played by the latex. Microscopic tests showed that SBR latex and SF could increase the content of calcium silicate hydrate (C-S-H) gel, inhibit the formation of ettringite (AFt) and reduce carbonation, refine the pore size of cement mortar, and effectively improve the microstructure of mortar.
    Language English
    Publishing date 2024-03-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527146-5
    ISSN 2073-4360 ; 2073-4360
    ISSN (online) 2073-4360
    ISSN 2073-4360
    DOI 10.3390/polym16050697
    Database MEDical Literature Analysis and Retrieval System OnLINE

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