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Article: Gene Therapy with p14/tBID Induces Selective and Synergistic Apoptosis in Mutant Ras and Mutant p53 Cancer Cells In Vitro and In Vivo.

Fine, Robert L / Mao, Yuehua / Garcia-Carracedo, Dario / Su, Gloria H / Qiu, Wanglong / Hochfeld, Uri / Nichols, Gwen / Li, Yong-Liang / Dinnen, Richard D / Raffo, Anthony / Brandt-Rauf, Paul W

Biomedicines

2023 Volume 11, Issue 2

Abstract: Any gene therapy for cancer will be predicated upon its selectivity against cancer cells and non-toxicity to normal cells. Therefore, safeguards are needed to prevent its activation in normal cells. We designed a minimal ... ...

Abstract	Any gene therapy for cancer will be predicated upon its selectivity against cancer cells and non-toxicity to normal cells. Therefore, safeguards are needed to prevent its activation in normal cells. We designed a minimal p14
Language	English
Publishing date	2023-01-18
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines11020258
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Article ; Online: Stathmin as a surrogate marker of phosphatidylinositol-3-kinase pathway activity

Andrew T. Turk / Dario Garcia-Carracedo / David T. Kent / Elizabeth Philipone / Juana Maria Garcia-Pedrero / Salvatore M. Caruana / Lanny G. Close / Gloria H. Su

Genes and Diseases, Vol 9, Iss 3, Pp 820-

Towards precision medicine in HPV-negative head & neck squamous cell carcinoma

2022 Volume 825

Abstract: In order to assess Stathmin as an immunohistochemical (IHC) indicator of phosphatidylinositol 3-kinase (PI3K) pathway activity in HPV-negative head & neck squamous cell carcinoma (HNSCC), we compared Stathmin IHC to expression of other pathway components. ...

Abstract	In order to assess Stathmin as an immunohistochemical (IHC) indicator of phosphatidylinositol 3-kinase (PI3K) pathway activity in HPV-negative head & neck squamous cell carcinoma (HNSCC), we compared Stathmin IHC to expression of other pathway components. We also evaluated the relationship between Stathmin IHC and the mutational status of four key pathway genes. Finally, we ascertained whether Stathmin IHC correlates with tumor grade or primary site. Correlation exists between high Stathmin expression and high pAKT1 expression, indicating a role for Stathmin IHC as a marker of pathway activity. Our analysis did not show correlation between Stathmin IHC and mutation of the four genes evaluated. We also observed an association between high Stathmin expression and oropharyngeal primary site. Our results suggest utility of Stathmin IHC as an indicator of PI3K pathway activity, and thereby demonstrate potential relevance of Stathmin IHC in the context of HNSCC.
Keywords	Cancer ; Head & ; neck squamous cell carcinoma ; Pathology ; Stathmin ; Medicine (General) ; R5-920 ; Genetics ; QH426-470
Subject code	570
Language	English
Publishing date	2022-05-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: PIK3CA

García-Carracedo, Darío / Cai, Yi / Qiu, Wanglong / Saeki, Kiyoshi / Friedman, Richard A / Lee, Andrew / Li, Yinglu / Goldberg, Elizabeth M / Stratikopoulos, Elias E / Parsons, Ramon / Lu, Chao / Efstratiadis, Argiris / Philipone, Elizabeth M / Yoon, Angela J / Su, Gloria H

Molecular cancer research : MCR

2020 Volume 18, Issue 6, Page(s) 822–834

Abstract: The PI3K signaling pathway is frequently mutated in head and neck squamous cell carcinoma (HNSCC), often via gain-of-function (GOF) mutations in ... ...

Abstract	The PI3K signaling pathway is frequently mutated in head and neck squamous cell carcinoma (HNSCC), often via gain-of-function (GOF) mutations in the
MeSH term(s)	4-Nitroquinoline-1-oxide/toxicity ; Animals ; Carcinogens/toxicity ; Class I Phosphatidylinositol 3-Kinases/genetics ; Disease Progression ; Head and Neck Neoplasms/chemically induced ; Head and Neck Neoplasms/genetics ; Head and Neck Neoplasms/pathology ; Mice ; Mice, Nude ; Mutation ; Squamous Cell Carcinoma of Head and Neck/chemically induced ; Squamous Cell Carcinoma of Head and Neck/genetics ; Squamous Cell Carcinoma of Head and Neck/secondary ; Tumor Suppressor Protein p53/genetics
Chemical Substances	Carcinogens ; Trp53 protein, mouse ; Tumor Suppressor Protein p53 ; 4-Nitroquinoline-1-oxide (56-57-5) ; Class I Phosphatidylinositol 3-Kinases (EC 2.7.1.137) ; Pik3ca protein, mouse (EC 2.7.1.137)
Language	English
Publishing date	2020-03-09
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2098788-2
ISSN	1557-3125 ; 1541-7786
ISSN (online)	1557-3125
ISSN	1541-7786
DOI	10.1158/1541-7786.MCR-19-0549
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Article ; Online: Stathmin as a surrogate marker of phosphatidylinositol-3-kinase pathway activity: Towards precision medicine in HPV-negative head & neck squamous cell carcinoma.

Turk, Andrew T / Garcia-Carracedo, Dario / Kent, David T / Philipone, Elizabeth / Garcia-Pedrero, Juana Maria / Caruana, Salvatore M / Close, Lanny G / Su, Gloria H

Genes & diseases

2020 Volume 9, Issue 3, Page(s) 820–825

Abstract	In order to assess Stathmin as an immunohistochemical (IHC) indicator of phosphatidylinositol 3-kinase (PI3K) pathway activity in HPV-negative head & neck squamous cell carcinoma (HNSCC), we compared Stathmin IHC to expression of other pathway components. We also evaluated the relationship between Stathmin IHC and the mutational status of four key pathway genes. Finally, we ascertained whether Stathmin IHC correlates with tumor grade or primary site. Correlation exists between high Stathmin expression and high pAKT1 expression, indicating a role for Stathmin IHC as a marker of pathway activity. Our analysis did not show correlation between Stathmin IHC and mutation of the four genes evaluated. We also observed an association between high Stathmin expression and oropharyngeal primary site. Our results suggest utility of Stathmin IHC as an indicator of PI3K pathway activity, and thereby demonstrate potential relevance of Stathmin IHC in the context of HNSCC.
Language	English
Publishing date	2020-12-13
Publishing country	China
Document type	Journal Article
ZDB-ID	2821806-1
ISSN	2352-3042 ; 2352-3042
ISSN (online)	2352-3042
ISSN	2352-3042
DOI	10.1016/j.gendis.2020.12.002
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Book ; Online: ADN forense: problemas éticos y jurídicos

Puig Marcó, Roser / Casado, María / Guillén, Margarita / Ortego, Ignacio Acón / Badia Martí, Anna / Feixat, Carme Barrot i / Bergel, Salvador Darío / Álvarez, Ángel Carracedo / Bidasolo, Mirentxu Corcoy / Fernández, Óscar García / Badia, Manel Gené / Ortega, Juan José López / Nadal, Gemma Marfany i / Yurrebaso, Iñaki / Prieto, Lourdes / Verdejo, Jaime Moreno / Michel, Mónica Navarro

2014

Abstract: Los análisis genéticos afectan a derechos fundamentales, por lo que el uso de esta información debe estar supeditado a vías de control democrático. En la actualidad, la lucha contra grandes delitos, como por ejemplo el terrorismo, aparentemente legitima ... ...

Abstract	Los análisis genéticos afectan a derechos fundamentales, por lo que el uso de esta información debe estar supeditado a vías de control democrático. En la actualidad, la lucha contra grandes delitos, como por ejemplo el terrorismo, aparentemente legitima a invadir derechos antes considerados intangibles. La presente obra, fruto del trabajo multidisciplinar llevado a cabo por juristas, filósofos, biólogos, técnicos y médicos, tiene por objetivo poner de manifiesto cuáles son los problemas ético-jurídicos derivados de la obtención, el análisis y el almacenamiento del ADN, así como sus usos judiciales y extrajudiciales. Ante el difícil equilibrio entre libertad individual y seguridad colectiva, este libro ayuda a comprender los conflictos que subyacen en el manejo de una herramienta informativa tan poderosa como son las muestras y los perfiles del ADN.
Keywords	Genetics ; Ethics
Size	1 electronic resource (320 p.)
Publisher	Publicacions i Edicions de la Universitat de Barcelona
Document type	Book ; Online
Note	Spanish ; Open Access
HBZ-ID	HT020099277
ISBN	9788447537945 ; 8447537943
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Article ; Online: Impact of PI3K/AKT/mTOR pathway activation on the prognosis of patients with head and neck squamous cell carcinomas.

García-Carracedo, Darío / Villaronga, Maria Ángeles / Álvarez-Teijeiro, Saúl / Hermida-Prado, Francisco / Santamaría, Iñigo / Allonca, Eva / Suárez-Fernández, Laura / Gonzalez, Maria Victoria / Balbín, Milagros / Astudillo, Aurora / Martínez-Camblor, Pablo / Su, Gloria H / Rodrigo, Juan Pablo / García-Pedrero, Juana María

Oncotarget

2016 Volume 7, Issue 20, Page(s) 29780–29793

Abstract: The PI3K/AKT/mTOR signaling pathway has emerged as one of the most frequently deregulated in head and neck squamous cell carcinomas (HNSCC). Numerous alterations of various upstream and downstream components have been described; however, their prognostic ...

Abstract	The PI3K/AKT/mTOR signaling pathway has emerged as one of the most frequently deregulated in head and neck squamous cell carcinomas (HNSCC). Numerous alterations of various upstream and downstream components have been described; however, their prognostic significance and impact on HNSCC patient survival remains to be established. This was addressed using an unbiased cohort of 93 consecutive and homogeneous surgically treated HNSCC patients and results confirmed in 432 HNSCC patients. Our findings reveal the high prevalence of S6 phosphorylation, a surrogate marker of mTORC1 activation, in HNSCC specimens (>70%) and, more importantly, demonstrate its relevance on clinical outcome. Phosphorylation of ribosomal protein S6 on either Ser235/236 or Ser240/244 was consistently and significantly correlated with favorable prognosis, although with differences depending on the tumor site. Thus, p-S6 expression was significantly correlated with better disease-specific survival specifically in the subgroup of laryngeal carcinoma patients (P< 0.001). In addition, multivariate regression models revealed p-S6 to be an inverse and independent predictor of lymph-node metastasis (P= 0.004) and distant metastasis (P= 0.006). Taken together, this study unveils an unprecedented correlation of mTOR activation with improved clinical outcome in patients with laryngeal carcinomas and uncovers the potential of p-S6 expression as a good prognostic biomarker and an inverse predictor of lymph node and distant metastases. These results should be of broad interest as immunohistochemical detection of p-S6 may help to stratify patients and guide treatment decisions.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Biomarkers, Tumor/analysis ; Biomarkers, Tumor/metabolism ; Carcinoma, Squamous Cell/metabolism ; Carcinoma, Squamous Cell/mortality ; Carcinoma, Squamous Cell/pathology ; Cohort Studies ; Disease-Free Survival ; Female ; Head and Neck Neoplasms/metabolism ; Head and Neck Neoplasms/mortality ; Head and Neck Neoplasms/pathology ; Humans ; Kaplan-Meier Estimate ; Male ; Middle Aged ; Phosphatidylinositol 3-Kinases/metabolism ; Prognosis ; Proto-Oncogene Proteins c-akt/metabolism ; Ribosomal Protein S6 Kinases/metabolism ; Squamous Cell Carcinoma of Head and Neck ; TOR Serine-Threonine Kinases/metabolism
Chemical Substances	Biomarkers, Tumor ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; MTOR protein, human (EC 2.7.1.1) ; TOR Serine-Threonine Kinases (EC 2.7.1.1) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Ribosomal Protein S6 Kinases (EC 2.7.11.1)
Language	English
Publishing date	2016-04-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	2560162-3
ISSN	1949-2553 ; 1949-2553
ISSN (online)	1949-2553
ISSN	1949-2553
DOI	10.18632/oncotarget.8957
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Article ; Online: First steps to creating a platform for high throughput simulation of TTFields.

Hershkovich, Hadas Sara / Bomzon, Zeev / Wenger, Cornelia / Urman, Noa / Chaudhry, Aafia / Garcia-Carracedo, Dario / Kirson, Eilon D / Weinberg, Uri / Wassermann, Yoram / Palti, Yoram

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference

2017 Volume 2016, Page(s) 2357–2360

Abstract: Tumor Treating Fields (TTFields) are low intensity alternating electric fields in the 100-500 KHz frequency range that are known to have an anti-mitotic effect on cancerous cells. In the USA, TTFields are approved by the Food and Drug Administration (FDA) ...

Abstract	Tumor Treating Fields (TTFields) are low intensity alternating electric fields in the 100-500 KHz frequency range that are known to have an anti-mitotic effect on cancerous cells. In the USA, TTFields are approved by the Food and Drug Administration (FDA) for the treatment of glioblastoma (GBM) in both the newly diagnosed and recurrent settings. Optimizing treatment with TTFields requires a deep understanding of how TTFields distribute within the brain. To address this issue, simulations using realistic head models have been performed. However, the preparation of such models is time-consuming and requires a high level of expertise, limiting the usefulness of these models for systematic studies in which the testing of multiple cases is required. Here we present a platform for rapidly simulating TTFields distributions in multiple scenarios. This platform enables high throughput computational simulations to be performed, allowing comparison of field distributions within the head in multiple clinically relevant scenarios. The simulation setup is simple and intuitive, allowing non-expert users to run simulations and evaluate results, thereby providing a valuable tool for studying how to optimize TTFields delivery in the clinic.
MeSH term(s)	Brain ; Brain Neoplasms/therapy ; Computer Simulation ; Electric Stimulation Therapy ; Electricity ; Glioblastoma/therapy ; Head ; Humans ; Models, Theoretical
Language	English
Publishing date	2017-03-08
Publishing country	United States
Document type	Journal Article
ISSN	2694-0604
ISSN (online)	2694-0604
DOI	10.1109/EMBC.2016.7591203
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Article ; Online: PIK3CA mutations in mucinous cystic neoplasms of the pancreas.

Garcia-Carracedo, Dario / Chen, Zong-Ming / Qiu, Wanglong / Huang, Alicia S / Tang, Sophia M / Hruban, Ralph H / Su, Gloria H

Pancreas

2014 Volume 43, Issue 2, Page(s) 245–249

Abstract: Objectives: Mucinous cystic neoplasms (MCNs) are rare, potentially curable, mucin-producing neoplasms of the pancreas. We have previously reported PIK3CA (phosphoinositide-3-kinase catalytic subunit, p110α) mutations in intraductal papillary mucinous ... ...

Abstract	Objectives: Mucinous cystic neoplasms (MCNs) are rare, potentially curable, mucin-producing neoplasms of the pancreas. We have previously reported PIK3CA (phosphoinositide-3-kinase catalytic subunit, p110α) mutations in intraductal papillary mucinous neoplasms, another mucin-producing neoplasm of the pancreas. In this study, we analyzed the presence of PIK3CA and AKT1/PKB (V-akt murine thymoma viral oncogene homolog 1) hot-spot mutations in MCN specimens. Methods: Using the genomic DNA sequencing of tumor tissues isolated by laser capture microdissection, we evaluated 15 well-characterized MCNs for the E542K, E545K (exon 9), and H1047R (exon 20) hot-spot mutations in the PIK3CA gene and the E17K mutation in the AKT1 gene. Results: A hot-spot mutation (E545K) of the PIK3CA gene was detected in 1 of the 15 MCNs and further confirmed by a mutant-enriched method. Interestingly, this mutation was found to be present only in the high-grade but not in low-grade dysplastic epithelium obtained from this neoplasm and coexisted with a KRAS mutation. No mutations were identified in the AKT1 gene. Conclusions: Our data, when combined with previous reports on intraductal papillary mucinous neoplasms, indicate that oncogenic activation of the PI3K pathway involving PIK3CA gene mutations can contribute to the progression of mucin-producing neoplasms but not pancreatic intraepithelial neoplasia. PIK3CA status could be useful for understanding their progression to malignancy.
MeSH term(s)	Adult ; Aged ; Class I Phosphatidylinositol 3-Kinases ; Cystadenocarcinoma, Mucinous/genetics ; Cystadenocarcinoma, Mucinous/pathology ; DNA Mutational Analysis ; Female ; Humans ; Middle Aged ; Mutation ; Pancreatic Neoplasms/genetics ; Pancreatic Neoplasms/pathology ; Phosphatidylinositol 3-Kinases/genetics ; Proto-Oncogene Proteins/genetics ; Proto-Oncogene Proteins c-akt/genetics ; Proto-Oncogene Proteins p21(ras) ; ras Proteins/genetics
Chemical Substances	KRAS protein, human ; Proto-Oncogene Proteins ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Class I Phosphatidylinositol 3-Kinases (EC 2.7.1.137) ; PIK3CA protein, human (EC 2.7.1.137) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Proto-Oncogene Proteins p21(ras) (EC 3.6.5.2) ; ras Proteins (EC 3.6.5.2)
Language	English
Publishing date	2014-02-08
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	632831-3
ISSN	1536-4828 ; 0885-3177
ISSN (online)	1536-4828
ISSN	0885-3177
DOI	10.1097/MPA.0000000000000034
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Zs.A 2160: Show issues

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Article ; Online: Podoplanin expression in the development and progression of laryngeal squamous cell carcinomas.

Rodrigo, Juan P / García-Carracedo, Dario / González, María V / Mancebo, Gonzalo / Fresno, Manuel F / García-Pedrero, Juana

Molecular cancer

2010 Volume 9, Page(s) 48

Abstract: Background: Podoplanin expression is attracting interest as a marker for cancer diagnosis and prognosis. We therefore investigated the expression pattern and clinical significance of podoplanin during the development and progression of laryngeal ... ...

Abstract	Background: Podoplanin expression is attracting interest as a marker for cancer diagnosis and prognosis. We therefore investigated the expression pattern and clinical significance of podoplanin during the development and progression of laryngeal carcinomas. Results: Podoplanin expression was determined by immunohistochemistry in paraffin-embedded tissue specimens from 84 patients with laryngeal premalignancies and 53 patients with laryngeal squamous cell carcinomas. We found podoplanin expression extending from the basal to the suprabasal layer of the epithelium in 37 (44%) of 84 dysplastic lesions, whereas normal epithelium showed negligible expression. Patients carrying podoplanin-positive lesions had a higher laryngeal cancer incidence than those with negative expression reaching borderline statistical significance (51% versus 30%, P = 0.071). Podoplanin expression in laryngeal carcinomas exhibited two distinct patterns. 20 (38%) cases showed diffuse expression in most tumour cells and 33 (62%) focal expression at the proliferating periphery of tumour nests. High podoplanin expression was inversely correlated with T classification (P = 0.033), disease stage (P = 0.006), and pathological grade (P = 0.04). There was a trend, although not significant, towards reduced disease-specific survival for patients with low podoplanin levels (P = 0.31) and diffuse expression pattern (P = 0.08). Conclusions: Podoplanin expression increases in the early stages of laryngeal tumourigenesis and it seems to be associated with a higher laryngeal cancer risk. Podoplanin expression in laryngeal squamous cell carcinomas, however, diminishes during tumour progression. Taken together, these data support a role for podoplanin expression in the initiation but not in the progression of laryngeal cancers.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Carcinoma, Squamous Cell/diagnosis ; Carcinoma, Squamous Cell/metabolism ; Carcinoma, Squamous Cell/pathology ; Disease Progression ; Disease-Free Survival ; Humans ; Immunohistochemistry ; Kaplan-Meier Estimate ; Laryngeal Neoplasms/diagnosis ; Laryngeal Neoplasms/metabolism ; Laryngeal Neoplasms/pathology ; Male ; Membrane Glycoproteins/metabolism ; Middle Aged ; Precancerous Conditions/metabolism ; Precancerous Conditions/pathology
Chemical Substances	Membrane Glycoproteins ; PDPN protein, human
Language	English
Publishing date	2010-03-02
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1476-4598
ISSN (online)	1476-4598
DOI	10.1186/1476-4598-9-48
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Article ; Online: Prognostic significance of lymphangiogenesis in pharyngolaryngeal carcinoma patients.

Garcia-Carracedo, Darío / Rodrigo, Juan Pablo / Astudillo, Aurora / Nieto, Carlos Suarez / Gonzalez, Maria Victoria

BMC cancer

2010 Volume 10, Page(s) 416

Abstract: Background: Lymphatic vessel spread is considered a major route for head and neck squamous cell carcinoma metastasis. Formation of new lymphatic vessels could facilitate the process, raising the malignant potential of these tumours. Recent ... ...

Abstract	Background: Lymphatic vessel spread is considered a major route for head and neck squamous cell carcinoma metastasis. Formation of new lymphatic vessels could facilitate the process, raising the malignant potential of these tumours. Recent identification of lymphatic markers allows the study of the lymphangiogenesis phenomenon. We searched for molecular events involved in the lymphangiogenic process that could have prognostic value in laryngeal/pharyngeal carcinoma patients. Methods: 104 paraffin-embedded pharyngeal/laryngeal tumour samples were studied. Immunohistochemical analysis of podoplanin and double immunofluorescence analysis of Ki-67 and D2-40 were performed. Lymph vessel density (inside the tumour mass, at its periphery or considered as a whole) and the presence of tumour emboli inside lymphatics were recorded. The proliferative state of endothelial lymphatic cells was evaluated. Results: Lymphatic vessels were detected inside the tumour mass (75%) and in the surrounding tissue (80%); some of them in a proliferative state. Tumour emboli were detected in a high proportion of the cases (45%). Lymphatic vessel density was higher in the pharyngeal cases (p = 0.0029), in greater size (p = 0.039), more advanced stage primary tumours (p = 0.006) and in carcinomas of patients with affected nodes (p = 0.019). The presence of tumour emboli and a high global vessel density were indicators of poor prognosis (recorded as death from tumour) in the laryngeal group (p = 0.015 and p = 0.027, respectively), but notably not in the pharyngeal one. Interestingly, high global vessel density showed a negative prognostic value among pathologically staged N0 laryngeal carcinomas (p = 0.03). Conclusions: The lymphangiogenic process correlated with aggressive tumour features (pN category, tumour size, tumour stage), but might play different roles in tumours arising from different anatomic sites. Our results suggest that detection of tumour emboli and assessment of global vessel density using the D2-40 antibody, may be useful in the clinical practice, as predictors of reduced survival among pN0 laryngeal carcinoma patients.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Antibodies, Monoclonal ; Antibodies, Monoclonal, Murine-Derived ; Carcinoma, Squamous Cell/metabolism ; Carcinoma, Squamous Cell/secondary ; Cell Proliferation ; DNA, Neoplasm/genetics ; Female ; Fluorescent Antibody Technique ; Humans ; Immunoenzyme Techniques ; Ki-67 Antigen/metabolism ; Laryngeal Neoplasms/metabolism ; Laryngeal Neoplasms/pathology ; Lymphangiogenesis ; Lymphatic Metastasis ; Lymphatic Vessels/metabolism ; Lymphatic Vessels/pathology ; Male ; Middle Aged ; Neoplasm Staging ; Pharyngeal Neoplasms/metabolism ; Pharyngeal Neoplasms/pathology ; Prognosis ; Survival Rate
Chemical Substances	Antibodies, Monoclonal ; Antibodies, Monoclonal, Murine-Derived ; DNA, Neoplasm ; Ki-67 Antigen ; monoclonal antibody D2-40
Language	English
Publishing date	2010-08-10
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2041352-X
ISSN	1471-2407 ; 1471-2407
ISSN (online)	1471-2407
ISSN	1471-2407
DOI	10.1186/1471-2407-10-416
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