LIVIVO - Search results -

Search results

Result 1 - 8 of total 8

Search options

Article ; Online: Data on isoaspartylation of neuronal ELAVL proteins

Mario A. Pulido / Meleeneh Kazarian DerHartunian / Prerna Sehgal / Ite A. Laird-Offringa

Data in Brief, Vol 9, Iss C, Pp 1052-

2016 Volume 1055

Abstract: This article contains experimental data examining the propensity of neuronal ELAVL proteins to become isoaspartylated. The data are related to the article “Isoaspartylation appears to trigger small cell lung cancer-associated autoimmunity against ... ...

Abstract	This article contains experimental data examining the propensity of neuronal ELAVL proteins to become isoaspartylated. The data are related to the article “Isoaspartylation appears to trigger small cell lung cancer-associated autoimmunity against neuronal protein ELAVL4” (M.A. Pulido, M.K. DerHartunian, Z. Qin, E.M. Chung, D.S. Kang, A.W. Woodham, J.A. Tsou, R. Klooster, O. Akbari, L. Wang, W.M. Kast, S.V. Liu, J.J.G.M. Verschuuren, D.W. Aswad, I.A. Laird-Offringa, 2016) [1], in which it was reported that the N-terminal region of recombinant human ELAVL4 protein, incubated under physiological conditions, acquires a type of highly immunogenic protein damage. Here, we present Western blot analysis data generated by using an affinity-purified polyclonal rabbit antibody (raised against an N-terminal ELAVL4 isoaspartyl-converted peptide) to probe recombinant protein fragments of the other three members of the ELAVL family: the highly homologous neuronal ELAVL2 (HuB) and ELAVL3 (HuC), and the much less homologous ubiquitously expressed ELAVL1 (HuR).
Keywords	Auto-antigens ; Autoimmunity ; ELAVL ; Isoaspartylation ; Neuronal proteins ; Small cell lung cancer ; SCLC ; Computer applications to medicine. Medical informatics ; R858-859.7 ; Science (General) ; Q1-390
Subject code	612
Language	English
Publishing date	2016-12-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Data on isoaspartylation of neuronal ELAVL proteins.

Pulido, Mario A / DerHartunian, Meleeneh Kazarian / Sehgal, Prerna / Laird-Offringa, Ite A

Data in brief

2016 Volume 9, Page(s) 1052–1055

Abstract: This article contains experimental data examining the propensity of neuronal ELAVL proteins to become isoaspartylated. The data are related to the article "Isoaspartylation appears to trigger small cell lung cancer-associated autoimmunity against ... ...

Abstract	This article contains experimental data examining the propensity of neuronal ELAVL proteins to become isoaspartylated. The data are related to the article "Isoaspartylation appears to trigger small cell lung cancer-associated autoimmunity against neuronal protein ELAVL4" (M.A. Pulido, M.K. DerHartunian, Z. Qin, E.M. Chung, D.S. Kang, A.W. Woodham, J.A. Tsou, R. Klooster, O. Akbari, L. Wang, W.M. Kast, S.V. Liu, J.J.G.M. Verschuuren, D.W. Aswad, I.A. Laird-Offringa, 2016) [1], in which it was reported that the N-terminal region of recombinant human ELAVL4 protein, incubated under physiological conditions, acquires a type of highly immunogenic protein damage. Here, we present Western blot analysis data generated by using an affinity-purified polyclonal rabbit antibody (raised against an N-terminal ELAVL4 isoaspartyl-converted peptide) to probe recombinant protein fragments of the other three members of the ELAVL family: the highly homologous neuronal ELAVL2 (HuB) and ELAVL3 (HuC), and the much less homologous ubiquitously expressed ELAVL1 (HuR).
Language	English
Publishing date	2016-11-17
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2786545-9
ISSN	2352-3409
ISSN	2352-3409
DOI	10.1016/j.dib.2016.11.034
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article: Data on isoaspartylation of neuronal ELAVL proteins

Pulido, Mario A. / DerHartunian, Meleeneh Kazarian / Sehgal, Prerna / Laird-Offringa, Ite A.

Data in Brief. 2016 Dec., v. 9

2016

Abstract	This article contains experimental data examining the propensity of neuronal ELAVL proteins to become isoaspartylated. The data are related to the article “Isoaspartylation appears to trigger small cell lung cancer-associated autoimmunity against neuronal protein ELAVL4” (M.A. Pulido, M.K. DerHartunian, Z. Qin, E.M. Chung, D.S. Kang, A.W. Woodham, J.A. Tsou, R. Klooster, O. Akbari, L. Wang, W.M. Kast, S.V. Liu, J.J.G.M. Verschuuren, D.W. Aswad, I.A. Laird-Offringa, 2016) [1], in which it was reported that the N-terminal region of recombinant human ELAVL4 protein, incubated under physiological conditions, acquires a type of highly immunogenic protein damage. Here, we present Western blot analysis data generated by using an affinity-purified polyclonal rabbit antibody (raised against an N-terminal ELAVL4 isoaspartyl-converted peptide) to probe recombinant protein fragments of the other three members of the ELAVL family: the highly homologous neuronal ELAVL2 (HuB) and ELAVL3 (HuC), and the much less homologous ubiquitously expressed ELAVL1 (HuR).
Keywords	Western blotting ; antibodies ; autoimmunity ; humans ; lungs ; neurons ; peptides ; rabbits ; recombinant proteins
Language	English
Dates of publication	2016-12
Size	p. 1052-1055.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	2786545-9
ISSN	2352-3409
ISSN	2352-3409
DOI	10.1016/j.dib.2016.11.034
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Small-cell lung cancer-associated autoantibodies: potential applications to cancer diagnosis, early detection, and therapy.

Kazarian, Meleeneh / Laird-Offringa, Ite A

Molecular cancer

2011 Volume 10, Page(s) 33

Abstract: Small-cell lung cancer (SCLC) is the most aggressive lung cancer subtype and lacks effective early detection methods and therapies. A number of rare paraneoplastic neurologic autoimmune diseases are strongly associated with SCLC. Most patients with such ... ...

Abstract	Small-cell lung cancer (SCLC) is the most aggressive lung cancer subtype and lacks effective early detection methods and therapies. A number of rare paraneoplastic neurologic autoimmune diseases are strongly associated with SCLC. Most patients with such paraneoplastic syndromes harbor high titers of antibodies against neuronal proteins that are abnormally expressed in SCLC tumors. These autoantibodies may cross-react with the nervous system, possibly contributing to autoimmune disease development. Importantly, similar antibodies are present in many SCLC patients without autoimmune disease, albeit at lower titers. The timing of autoantibody development relative to cancer and the nature of the immune trigger remain to be elucidated. Here we review what is currently known about SCLC-associated autoantibodies, and describe a recently developed mouse model system of SCLC that appears to lend itself well to the study of the SCLC-associated immune response. We also discuss potential clinical applications for these autoantibodies, such as SCLC diagnosis, early detection, and therapy.
MeSH term(s)	Animals ; Autoantibodies/immunology ; Autoimmunity/immunology ; Disease Models, Animal ; Early Diagnosis ; Humans ; Lung Neoplasms/diagnosis ; Lung Neoplasms/immunology ; Lung Neoplasms/therapy ; Mice ; Small Cell Lung Carcinoma/diagnosis ; Small Cell Lung Carcinoma/immunology ; Small Cell Lung Carcinoma/therapy
Chemical Substances	Autoantibodies
Language	English
Publishing date	2011-03-30
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
ISSN	1476-4598
ISSN (online)	1476-4598
DOI	10.1186/1476-4598-10-33
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article ; Online: Small-cell lung cancer-associated autoantibodies

Laird-Offringa Ite A / Kazarian Meleeneh

Molecular Cancer, Vol 10, Iss 1, p

potential applications to cancer diagnosis, early detection, and therapy

2011 Volume 33

Abstract: Abstract Small-cell lung cancer (SCLC) is the most aggressive lung cancer subtype and lacks effective early detection methods and therapies. A number of rare paraneoplastic neurologic autoimmune diseases are strongly associated with SCLC. Most patients ... ...

Abstract	Abstract Small-cell lung cancer (SCLC) is the most aggressive lung cancer subtype and lacks effective early detection methods and therapies. A number of rare paraneoplastic neurologic autoimmune diseases are strongly associated with SCLC. Most patients with such paraneoplastic syndromes harbor high titers of antibodies against neuronal proteins that are abnormally expressed in SCLC tumors. These autoantibodies may cross-react with the nervous system, possibly contributing to autoimmune disease development. Importantly, similar antibodies are present in many SCLC patients without autoimmune disease, albeit at lower titers. The timing of autoantibody development relative to cancer and the nature of the immune trigger remain to be elucidated. Here we review what is currently known about SCLC-associated autoantibodies, and describe a recently developed mouse model system of SCLC that appears to lend itself well to the study of the SCLC-associated immune response. We also discuss potential clinical applications for these autoantibodies, such as SCLC diagnosis, early detection, and therapy.
Keywords	Neoplasms. Tumors. Oncology. Including cancer and carcinogens ; RC254-282 ; Internal medicine ; RC31-1245 ; Medicine ; R ; DOAJ:Oncology ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
Subject code	610
Language	English
Publishing date	2011-03-01T00:00:00Z
Publisher	BioMed Central
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Immune response in lung cancer mouse model mimics human anti-Hu reactivity.

Kazarian, Meleeneh / Calbo, Joaquim / Proost, Natalie / Carpenter, Catherine L / Berns, Anton / Laird-Offringa, Ite A

Journal of neuroimmunology

2009 Volume 217, Issue 1-2, Page(s) 38–45

Abstract: Most patients with paraneoplastic encephalomyelitis/sensory neuronopathy PEM/SN have small-cell lung cancer (SCLC) and develop antibodies against neuronal-specific Hu proteins, which are abnormally expressed in the tumor. Anti-Hu reactivity is present in ...

Abstract	Most patients with paraneoplastic encephalomyelitis/sensory neuronopathy PEM/SN have small-cell lung cancer (SCLC) and develop antibodies against neuronal-specific Hu proteins, which are abnormally expressed in the tumor. Anti-Hu reactivity is present in ~16% of SCLC patients without PEM/SN. Here we test the hypothesis that engineered SCLC-prone mice may exhibit anti-Hu reactivity. We show that tumors from SCLC-prone mice misexpress Hu proteins, and 14% of mice harbor anti-Hu antibodies. Mice appear to show reactivity prior to clinical diagnosis of SCLC. This mouse model system will be useful to study SCLC-associated autoimmunity, its diagnostic value, and the potential protective role of oncoantigen-directed autoantibodies.
MeSH term(s)	Animals ; Antibodies/blood ; Autoantibodies/immunology ; Autoantibodies/metabolism ; Disease Models, Animal ; ELAV Proteins/genetics ; ELAV Proteins/immunology ; Humans ; Luciferases/genetics ; Lung Neoplasms/blood ; Lung Neoplasms/immunology ; Lung Neoplasms/mortality ; Magnetic Resonance Imaging ; Mice ; Neoplasm Transplantation/immunology ; RNA, Messenger/metabolism ; Small Cell Lung Carcinoma/blood ; Small Cell Lung Carcinoma/immunology ; Small Cell Lung Carcinoma/mortality ; Survival Analysis ; Time Factors ; Vaccines, DNA/immunology
Chemical Substances	Antibodies ; Autoantibodies ; ELAV Proteins ; RNA, Messenger ; Vaccines, DNA ; Luciferases (EC 1.13.12.-)
Language	English
Publishing date	2009-09-17
Publishing country	Netherlands
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	8335-5
ISSN	1872-8421 ; 0165-5728
ISSN (online)	1872-8421
ISSN	0165-5728
DOI	10.1016/j.jneuroim.2009.08.014
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1646: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Isoaspartylation appears to trigger small cell lung cancer-associated autoimmunity against neuronal protein ELAVL4.

Pulido, Mario A / DerHartunian, Meleeneh Kazarian / Qin, Zhenxia / Chung, Eric M / Kang, Diane S / Woodham, Andrew W / Tsou, Jeffrey A / Klooster, Rinse / Akbari, Omid / Wang, Lina / Kast, W Martin / Liu, Stephen V / Verschuuren, Jan J G M / Aswad, Dana W / Laird-Offringa, Ite A

Journal of neuroimmunology

2016 Volume 299, Page(s) 70–78

Abstract: Autoantibodies against SCLC-associated neuronal antigen ELAVL4 (HuD) have been linked to smaller tumors and improved survival, but the antigenic epitope and mechanism of autoimmunity have never been solved. We report that recombinant human ELAVL4 protein ...

Abstract	Autoantibodies against SCLC-associated neuronal antigen ELAVL4 (HuD) have been linked to smaller tumors and improved survival, but the antigenic epitope and mechanism of autoimmunity have never been solved. We report that recombinant human ELAVL4 protein incubated under physiological conditions acquires isoaspartylation, a type of immunogenic protein damage. Specifically, the N-terminal region of ELAVL4, previously implicated in SCLC-associated autoimmunity, undergoes isoaspartylation in vitro, is recognized by sera from anti-ELAVL4 positive SCLC patients and is highly immunogenic in subcutaneously injected mice and in vitro stimulated human lymphocytes. Our data suggest that isoaspartylated ELAVL4 is the trigger for the SCLC-associated anti-ELAVL4 autoimmune response.
MeSH term(s)	Adult ; Aged ; Amino Acid Sequence ; Animals ; Autoimmunity/immunology ; ELAV-Like Protein 4/genetics ; ELAV-Like Protein 4/immunology ; ELAV-Like Protein 4/metabolism ; Female ; Humans ; Lung Neoplasms/genetics ; Lung Neoplasms/immunology ; Lung Neoplasms/metabolism ; Male ; Mice ; Mice, Knockout ; Middle Aged ; Neurons/immunology ; Neurons/metabolism ; Rabbits ; Small Cell Lung Carcinoma/genetics ; Small Cell Lung Carcinoma/immunology ; Small Cell Lung Carcinoma/metabolism
Chemical Substances	ELAV-Like Protein 4 ; ELAVL4 protein, human
Language	English
Publishing date	2016-09-03
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural
ZDB-ID	8335-5
ISSN	1872-8421 ; 0165-5728
ISSN (online)	1872-8421
ISSN	0165-5728
DOI	10.1016/j.jneuroim.2016.09.002
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1646: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Low level anti-Hu reactivity: A risk marker for small cell lung cancer?

Tsou, Jeffrey A / Kazarian, Meleeneh / Patel, Ankur / Galler, Janice S / Laird-Offringa, Ite A / Carpenter, Catherine L / London, Stephanie J

Cancer detection and prevention

2008 Volume 32, Issue 4, Page(s) 292–299

Abstract: Background: Previous experimental and laboratory studies have implicated antibodies against Hu proteins (anti-Hu) as a potential marker for small cell lung cancer (SCLC); there are no estimates of the association between anti-Hu and SCLC using a ... ...

Abstract	Background: Previous experimental and laboratory studies have implicated antibodies against Hu proteins (anti-Hu) as a potential marker for small cell lung cancer (SCLC); there are no estimates of the association between anti-Hu and SCLC using a population-based design. Methods: We used stored plasma specimens to evaluate anti-Hu reactivity in relationship to small cell lung cancer in a population-based case-control study. Using Western Blot analysis, we measured anti-Hu reactivity against recombinant Hu family member, HuD, in plasma samples from 41 SCLC cases and 79 controls individually matched for age, race, sex, and smoking status (never, past, current). We analyzed the association between anti-Hu reactivity and SCLC using conditional logistic regression. Results: Anti-Hu reactivity was associated with SCLC, both before and after adjustment for amount of smoking. We observed a smoking-adjusted odds ratio of 3.2 (95% confidence interval from 0.98 to 13.4) comparing subjects above 1800 units (the lower limit of the second tertile of the distribution among antibody positive controls) to subjects with lower reactivity. We also found suggestive evidence in follow-up of our cases that anti-Hu above 1800 units was related to longer-term survival from SCLC. The present research is the first report of anti-Hu reactivity and SCLC in a population-based study. Conclusions: Given the suggestive evidence in this study, prospective analyses to examine whether anti-Hu reactivity might predict risk of developing SCLC, or whether anti-Hu reactivity could serve as an early marker for SCLC, may be warranted.
MeSH term(s)	African Americans ; Aged ; Antibody Formation ; Autoantibodies/blood ; Biomarkers, Tumor/blood ; Biomarkers, Tumor/immunology ; Case-Control Studies ; ELAV Proteins/blood ; ELAV Proteins/immunology ; European Continental Ancestry Group ; Female ; Follow-Up Studies ; Humans ; Lung Neoplasms/blood ; Lung Neoplasms/immunology ; Lung Neoplasms/mortality ; Male ; Middle Aged ; Risk Factors ; Small Cell Lung Carcinoma/blood ; Small Cell Lung Carcinoma/immunology ; Small Cell Lung Carcinoma/mortality ; Smoking/blood ; Smoking/immunology ; Survival Analysis
Chemical Substances	Autoantibodies ; Biomarkers, Tumor ; ELAV Proteins
Language	English
Publishing date	2008-12-12
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, N.I.H., Intramural ; Research Support, Non-U.S. Gov't
ZDB-ID	425808-3
ISSN	1525-1500 ; 0361-090X
ISSN (online)	1525-1500
ISSN	0361-090X
DOI	10.1016/j.cdp.2008.06.006
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1427: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito