Article ; Online: Evaluation of organophosphorus pesticide tyrosine adducts for postmortem change by human serum albumin with liquid chromatography quadrupole Orbitrap mass spectrometry.
Toxicological sciences : an official journal of the Society of Toxicology
2024 Volume 199, Issue 1, Page(s) 40–48
Abstract: Organophosphorus pesticides (OPPs) having a phosphate ester moiety, such as malathion (MA) and methidathion (DMTP), are widely used and have been detected in many fatal cases of accidental exposure or suicide in Japan. In forensic toxicology, the ... ...
Abstract | Organophosphorus pesticides (OPPs) having a phosphate ester moiety, such as malathion (MA) and methidathion (DMTP), are widely used and have been detected in many fatal cases of accidental exposure or suicide in Japan. In forensic toxicology, the accurate determination of blood OPP concentration is mandatory to prove death by OPP poisoning. However, fatal pesticide concentration in blood at autopsy varies depending on the circumstances surrounding the dead body. In this study, we found that 16 OPPs were degraded by human serum albumin (HSA) in a temperature-dependent fashion. The mechanism underlying MA, DMTP, azinphos-methyl, etrimfos, fenthion (MPP), pirimiphos-methyl, (E)-dimethylvinphos, (Z)-dimethylvinphos, vamidothion, edifenphos (EDDP), fosthiazate, and pyraclofos degradation involves the formation of adducts with tyrosine residues in HSA. The mass spectra obtained by liquid chromatography quadrupole Orbitrap mass spectrometry revealed that phosphate ester amino acid adducts such as Y-adduct1, Y-adduct2, Y-adduct3, Y-adduct4, and Y-adduct5 were formed in HSA solution incubated with OPPs. These results indicate that the 16 OPPs are postmortem changed by HSA. The detection of phosphate ester amino acid adducts such as Y-adduct1, Y-adduct2, Y-adduct3, Y-adduct4, and Y-adduct5, instead of MA, DMTP, azinphos-methyl, etrimfos, MPP, pirimiphos-methyl, (E)-dimethylvinphos, (Z)-dimethylvinphos, vamidothion, EDDP, fosthiazate, and pyraclofos per se, may be used to determine death by these OPPs poisoning. |
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MeSH term(s) | Humans ; Organophosphorus Compounds/toxicity ; Organophosphorus Compounds/chemistry ; Tyrosine/analogs & derivatives ; Pesticides/toxicity ; Pesticides/chemistry ; Serum Albumin, Human/chemistry ; Postmortem Changes ; Chromatography, Liquid ; Mass Spectrometry/methods ; Forensic Toxicology/methods |
Chemical Substances | Organophosphorus Compounds ; Tyrosine (42HK56048U) ; Pesticides ; Serum Albumin, Human (ZIF514RVZR) |
Language | English |
Publishing date | 2024-02-17 |
Publishing country | United States |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 1420885-4 |
ISSN | 1096-0929 ; 1096-6080 |
ISSN (online) | 1096-0929 |
ISSN | 1096-6080 |
DOI | 10.1093/toxsci/kfae023 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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