Article: Effect of N(G)-nitro-L-arginine methyl ester on functionally characterized muscarinic receptors in anesthetized cats.
European journal of pharmacology
1997 Volume 335, Issue 2-3, Page(s) 199–204
Abstract: This study was undertaken to determine if the nitric oxide (NO) synthase inhibitor, NG-nitro-L ... arginine methyl ester (L-NAME), is a competitive antagonist of muscarinic receptors in vivo. Cats were ... groups of animals were administered L-NAME (50 mg/kg, i.v.) to determine if this agent would alter ...
| Abstract | This study was undertaken to determine if the nitric oxide (NO) synthase inhibitor, NG-nitro-L-arginine methyl ester (L-NAME), is a competitive antagonist of muscarinic receptors in vivo. Cats were anesthetized with pentobarbital (36 mg/kg, i.p.). Five peripheral muscarinic responses were characterized based on their sensitivity to intravenous administration of atropine (1-100 microg/kg), pirenzepine (1-100 microg/kg) or gallamine (30-3000 microg/kg) as follows: (1) muscarinic ganglionic transmission through the superior cervical ganglion to the nictitating membrane (M1), (2) electrically elicited vagal bradycardia (M2), (3) neurally evoked sudomotor responses (M3; non-endothelial), (4) basal pupil tone in sympathectomized cats (M3; non-endothelial) and (5) methacholine-induced depression of arterial blood pressure (M3; endothelial). Additional groups of animals were administered L-NAME (50 mg/kg, i.v.) to determine if this agent would alter activation of these muscarinic systems. L-NAME was devoid of effect on responses elicited by stimulation of muscarinic M1, M2 and M3 (non-endothelial) receptors. In contrast, L-NAME significantly reduced the depressor responses to i.v. methacholine (M3; endothelial), as did its non-alkyl ester congener, L-NA (NG-nitro-L-arginine; 25 mg/kg, i.v.). These results support the conclusion that although L-NAME inhibits synthesis of nitric oxide in vascular endothelial cells, it is not a generalized muscarinic receptor antagonist in vivo. |
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| MeSH term(s) | Animals ; Atropine/pharmacology ; Cats ; Enzyme Inhibitors/pharmacology ; Gallamine Triethiodide/pharmacology ; Muscarinic Antagonists/pharmacology ; NG-Nitroarginine Methyl Ester/pharmacology ; Nitric Oxide Synthase/antagonists & inhibitors ; Nitroarginine/pharmacology ; Pirenzepine/pharmacology ; Receptor, Muscarinic M1 ; Receptor, Muscarinic M2 ; Receptor, Muscarinic M3 ; Receptors, Muscarinic/drug effects |
| Chemical Substances | Enzyme Inhibitors ; Muscarinic Antagonists ; Receptor, Muscarinic M1 ; Receptor, Muscarinic M2 ; Receptor, Muscarinic M3 ; Receptors, Muscarinic ; Nitroarginine (2149-70-4) ; Pirenzepine (3G0285N20N) ; Atropine (7C0697DR9I) ; Nitric Oxide Synthase (EC 1.14.13.39) ; Gallamine Triethiodide (Q3254X40X2) ; NG-Nitroarginine Methyl Ester (V55S2QJN2X) |
| Language | English |
| Publishing date | 1997-09-24 |
| Publishing country | Netherlands |
| Document type | Comparative Study ; Journal Article ; Research Support, U.S. Gov't, P.H.S. |
| ZDB-ID | 80121-5 |
| ISSN | 1879-0712 ; 0014-2999 |
| ISSN (online) | 1879-0712 |
| ISSN | 0014-2999 |
| DOI | 10.1016/s0014-2999(97)01236-3 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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