Result 1 - 10 of total 12
Environ Ecol Stat
| Keywords | covid19 |
|---|---|
| Publisher | PMC |
| Document type | Article ; Online |
| DOI | 10.1007/s10651-020-00474-0 |
| Database | COVID19 |
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2023 Volume 12
Abstract: The escape trajectory (ET) of prey - measured as the angle relative to the predator's approach path - plays a major role in avoiding predation. Previous geometric models predict a single ET; however, many species show highly variable ETs with multiple ... ...
| Abstract | The escape trajectory (ET) of prey - measured as the angle relative to the predator's approach path - plays a major role in avoiding predation. Previous geometric models predict a single ET; however, many species show highly variable ETs with multiple preferred directions. Although such a high ET variability may confer unpredictability to avoid predation, the reasons why animals prefer specific multiple ETs remain unclear. Here, we constructed a novel geometric model that incorporates the time required for prey to turn and the predator's position at the end of its attack. The optimal ET was determined by maximizing the time difference of arrival at the edge of the safety zone between the prey and predator. By fitting the model to the experimental data of fish |
|---|---|
| MeSH term(s) | Animals ; Escape Reaction ; Predatory Behavior ; Anura |
| Language | English |
| Publishing date | 2023-02-15 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2687154-3 |
| ISSN | 2050-084X ; 2050-084X |
| ISSN (online) | 2050-084X |
| ISSN | 2050-084X |
| DOI | 10.7554/eLife.77699 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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eLife, Vol
2023 Volume 12
Abstract: The escape trajectory (ET) of prey – measured as the angle relative to the predator’s approach path – plays a major role in avoiding predation. Previous geometric models predict a single ET; however, many species show highly variable ETs with multiple ... ...
| Abstract | The escape trajectory (ET) of prey – measured as the angle relative to the predator’s approach path – plays a major role in avoiding predation. Previous geometric models predict a single ET; however, many species show highly variable ETs with multiple preferred directions. Although such a high ET variability may confer unpredictability to avoid predation, the reasons why animals prefer specific multiple ETs remain unclear. Here, we constructed a novel geometric model that incorporates the time required for prey to turn and the predator’s position at the end of its attack. The optimal ET was determined by maximizing the time difference of arrival at the edge of the safety zone between the prey and predator. By fitting the model to the experimental data of fish Pagrus major, we show that the model can clearly explain the observed multiple preferred ETs. By changing the parameters of the same model within a realistic range, we were able to produce various patterns of ETs empirically observed in other species (e.g., insects and frogs): a single preferred ET and multiple preferred ETs at small (20–50°) and large (150–180°) angles from the predator. Our results open new avenues of investigation for understanding how animals choose their ETs from behavioral and neurosensory perspectives. |
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| Keywords | escape direction ; escape response ; escape turn ; matching law ; mathematical model ; predator evasion ; Medicine ; R ; Science ; Q ; Biology (General) ; QH301-705.5 |
| Subject code | 590 |
| Language | English |
| Publishing date | 2023-02-01T00:00:00Z |
| Publisher | eLife Sciences Publications Ltd |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
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The Plant journal : for cell and molecular biology
2022 Volume 112, Issue 6, Page(s) 1337–1349
Abstract: Structure-based high-throughput screening of chemical compounds that target protein-protein interactions (PPIs) is a promising technology for gaining insight into how plant development is regulated, leading to many potential agricultural applications. At ...
| Abstract | Structure-based high-throughput screening of chemical compounds that target protein-protein interactions (PPIs) is a promising technology for gaining insight into how plant development is regulated, leading to many potential agricultural applications. At present, there are no examples of using high-throughput screening to identify chemicals that target plant transcriptional complexes, some of which are responsible for regulating multiple physiological functions. Florigen, a protein encoded by FLOWERING LOCUS T (FT), was initially identified as a molecule that promotes flowering and has since been shown to regulate flowering and other developmental phenomena such as tuber formation in potato (Solanum tuberosum). FT functions as a component of the florigen activation complex (FAC) with a 14-3-3 scaffold protein and FD, a bZIP transcription factor that activates downstream gene expression. Although 14-3-3 is an important component of FAC, little is known about the function of the 14-3-3 protein itself. Here, we report the results of a high-throughput in vitro fluorescence resonance energy transfer (FRET) screening of chemical libraries that enabled us to identify small molecules capable of inhibiting FAC formation. These molecules abrogate the in vitro interaction between the 14-3-3 protein and the OsFD1 peptide, a rice (Oryza sativa) FD, by directly binding to the 14-3-3 protein. Treatment with S4, a specific hit molecule, strongly inhibited FAC activity and flowering in duckweed, tuber formation in potato, and branching in rice in a dose-dependent manner. Our results demonstrate that the high-throughput screening approach based on the three-dimensional structure of PPIs is suitable in plants. In this study, we have proposed good candidate compounds for future modification to obtain inhibitors of florigen-dependent processes through inhibition of FAC formation. |
|---|---|
| MeSH term(s) | Florigen/metabolism ; Plant Proteins/metabolism ; 14-3-3 Proteins/genetics ; 14-3-3 Proteins/metabolism ; High-Throughput Screening Assays ; Oryza/metabolism ; Gene Expression Regulation, Plant ; Flowers/genetics |
| Chemical Substances | Florigen ; Plant Proteins ; 14-3-3 Proteins |
| Language | English |
| Publishing date | 2022-11-16 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 1088037-9 |
| ISSN | 1365-313X ; 0960-7412 |
| ISSN (online) | 1365-313X |
| ISSN | 0960-7412 |
| DOI | 10.1111/tpj.16008 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Z 6071: Show issues |
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Plant biotechnology (Tokyo, Japan)
2021 Volume 38, Issue 1, Page(s) 89–99
Abstract: Luciferases have been widely utilized as sensitive reporters to monitor gene expression and protein-protein interactions. Compared to firefly luciferase (Fluc), a recently developed luciferase, Nanoluciferase (NanoLuc or Nluc), has several superior ... ...
| Abstract | Luciferases have been widely utilized as sensitive reporters to monitor gene expression and protein-protein interactions. Compared to firefly luciferase (Fluc), a recently developed luciferase, Nanoluciferase (NanoLuc or Nluc), has several superior properties such as a smaller size and stronger luminescence activity. We compared the reporter properties of Nluc and Fluc in rice ( |
|---|---|
| Language | English |
| Publishing date | 2021-06-18 |
| Publishing country | Japan |
| Document type | Journal Article |
| ZDB-ID | 2210142-1 |
| ISSN | 1347-6114 ; 1342-4580 |
| ISSN (online) | 1347-6114 |
| ISSN | 1342-4580 |
| DOI | 10.5511/plantbiotechnology.20.1209a |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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The Plant Journal. 2022 Dec., v. 112, no. 6 p.1337-1349
2022
Abstract: Structure‐based high‐throughput screening of chemical compounds that target protein–protein interactions (PPIs) is a promising technology for gaining insight into how plant development is regulated, leading to many potential agricultural applications. At ...
| Abstract | Structure‐based high‐throughput screening of chemical compounds that target protein–protein interactions (PPIs) is a promising technology for gaining insight into how plant development is regulated, leading to many potential agricultural applications. At present, there are no examples of using high‐throughput screening to identify chemicals that target plant transcriptional complexes, some of which are responsible for regulating multiple physiological functions. Florigen, a protein encoded by FLOWERING LOCUS T (FT), was initially identified as a molecule that promotes flowering and has since been shown to regulate flowering and other developmental phenomena such as tuber formation in potato (Solanum tuberosum). FT functions as a component of the florigen activation complex (FAC) with a 14‐3‐3 scaffold protein and FD, a bZIP transcription factor that activates downstream gene expression. Although 14‐3‐3 is an important component of FAC, little is known about the function of the 14‐3‐3 protein itself. Here, we report the results of a high‐throughput in vitro fluorescence resonance energy transfer (FRET) screening of chemical libraries that enabled us to identify small molecules capable of inhibiting FAC formation. These molecules abrogate the in vitro interaction between the 14‐3‐3 protein and the OsFD1 peptide, a rice (Oryza sativa) FD, by directly binding to the 14‐3‐3 protein. Treatment with S4, a specific hit molecule, strongly inhibited FAC activity and flowering in duckweed, tuber formation in potato, and branching in rice in a dose‐dependent manner. Our results demonstrate that the high‐throughput screening approach based on the three‐dimensional structure of PPIs is suitable in plants. In this study, we have proposed good candidate compounds for future modification to obtain inhibitors of florigen‐dependent processes through inhibition of FAC formation. |
|---|---|
| Keywords | Araceae ; Oryza sativa ; Solanum tuberosum ; dose response ; energy transfer ; florigen ; fluorescence ; gene expression ; loci ; peptides ; plant development ; potatoes ; rice ; scaffolding proteins ; transcription (genetics) ; transcription factors |
| Language | English |
| Dates of publication | 2022-12 |
| Size | p. 1337-1349. |
| Publishing place | John Wiley & Sons, Ltd |
| Document type | Article ; Online |
| Note | JOURNAL ARTICLE |
| ZDB-ID | 1088037-9 |
| ISSN | 1365-313X ; 0960-7412 |
| ISSN (online) | 1365-313X |
| ISSN | 0960-7412 |
| DOI | 10.1111/tpj.16008 |
| Database | NAL-Catalogue (AGRICOLA) |
| Z 6071: Show issues |
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2023 Volume 99, Issue 2, Page(s) 193–203.e5
Abstract: Background and aims: We compared ERCP using a balloon-assisted endoscope (BE-ERCP) with EUS-guided antegrade treatment (EUS-AG) for removal of common bile duct (CBD) stones in patients with Roux-en-Y (R-Y) gastrectomy.: Methods: Consecutive patients ... ...
| Abstract | Background and aims: We compared ERCP using a balloon-assisted endoscope (BE-ERCP) with EUS-guided antegrade treatment (EUS-AG) for removal of common bile duct (CBD) stones in patients with Roux-en-Y (R-Y) gastrectomy. Methods: Consecutive patients who had previous R-Y gastrectomy undergoing BE-ERCP or EUS-AG for CBD stones in 16 centers were retrospectively analyzed. Results: BE-ERCP and EUS-AG were performed in 588 and 59 patients, respectively. Baseline characteristics were similar, except for CBD diameter and angle. The technical success rate was 83.7% versus 83.1% (P = .956), complete stone removal rate was 78.1% versus 67.8% (P = .102), and early adverse event rate was 10.2% versus 18.6% (P = .076) in BE-ERCP and EUS-AG, respectively. The mean number of endoscopic sessions was smaller in BE-ERCP (1.5 ± .8 vs 1.9 ± 1.0 sessions, P = .01), whereas the median total treatment time was longer (90 vs 61.5 minutes, P = .001). Among patients with biliary access, the complete stone removal rate was significantly higher in BE-ERCP (93.3% vs 81.6%, P = .009). Negative predictive factors were CBD diameter ≥15 mm (odds ratio [OR], .41) and an angle of CBD <90 degrees (OR, .39) in BE-ERCP and a stone size ≥10 mm (OR, .07) and an angle of CBD <90 degrees (OR, .07) in EUS-AG. The 1-year recurrence rate was 8.3% in both groups. Conclusions: Effectiveness and safety of BE-ERCP and EUS-AG were comparable in CBD stone removal for patients after R-Y gastrectomy, but complete stone removal after technical success was superior in BE-ERCP. |
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| MeSH term(s) | Humans ; Cholangiopancreatography, Endoscopic Retrograde/adverse effects ; Retrospective Studies ; Gallstones/diagnostic imaging ; Gallstones/surgery ; Gastrectomy ; Common Bile Duct ; Endoscopes ; Treatment Outcome |
| Language | English |
| Publishing date | 2023-09-13 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 391583-9 |
| ISSN | 1097-6779 ; 0016-5107 |
| ISSN (online) | 1097-6779 |
| ISSN | 0016-5107 |
| DOI | 10.1016/j.gie.2023.09.001 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Zs.B 283: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG) |
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| Zs.MO 519: Show issues |
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PloS one
2012 Volume 7, Issue 11, Page(s) e50309
Abstract: To analyze an animal's movement trajectory, a basic model is required that satisfies the following conditions: the model must have an ecological basis and the parameters used in the model must have ecological interpretations, a broad range of movement ... ...
| Abstract | To analyze an animal's movement trajectory, a basic model is required that satisfies the following conditions: the model must have an ecological basis and the parameters used in the model must have ecological interpretations, a broad range of movement patterns can be explained by that model, and equations and probability distributions in the model should be mathematically tractable. Random walk models used in previous studies do not necessarily satisfy these requirements, partly because movement trajectories are often more oriented or tortuous than expected from the models. By improving the modeling for turning angles, this study aims to propose a basic movement model. On the basis of the recently developed circular auto-regressive model, we introduced a new movement model and extended its applicability to capture the asymmetric effects of external factors such as wind. The model was applied to GPS trajectories of a seabird (Calonectris leucomelas) to demonstrate its applicability to various movement patterns and to explain how the model parameters are ecologically interpreted under a general conceptual framework for movement ecology. Although it is based on a simple extension of a generalized linear model to circular variables, the proposed model enables us to evaluate the effects of external factors on movement separately from the animal's internal state. For example, maximum likelihood estimates and model selection suggested that in one homing flight section, the seabird intended to fly toward the island, but misjudged its navigation and was driven off-course by strong winds, while in the subsequent flight section, the seabird reset the focal direction, navigated the flight under strong wind conditions, and succeeded in approaching the island. |
|---|---|
| MeSH term(s) | Animal Migration ; Animals ; Birds/physiology ; Ecology ; Flight, Animal/physiology ; Islands ; Likelihood Functions ; Male ; Models, Biological ; Movement ; Oceans and Seas ; Wind |
| Language | English |
| Publishing date | 2012-11-30 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ISSN | 1932-6203 |
| ISSN (online) | 1932-6203 |
| DOI | 10.1371/journal.pone.0050309 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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Plant physiology and biochemistry. 2018 Oct., v. 131 p.78-83
2018
Abstract: The CRISPR/Cas9 system is a revolutionary genome-editing tool for directed gene editing in various organisms. Cas9 variants can be applied as molecular homing devices when combined with various functional effectors such as transcriptional activators or ... ...
| Abstract | The CRISPR/Cas9 system is a revolutionary genome-editing tool for directed gene editing in various organisms. Cas9 variants can be applied as molecular homing devices when combined with various functional effectors such as transcriptional activators or DNA modification enzymes. Target-AID is a synthetic complex of nuclease deficient Cas9 fused to an activation-induced cytidine deaminase (AID) that enables targeted nucleotide substitution (C to T or G to A). We previously demonstrated that the introduction of desired point mutations into target genes by Target-AID confers herbicide tolerance to rice callus. Inheritance of the introduced mutations, as well as the removal of transgenes, are key issues that must be addressed in order to fully develop Target-AID as a plant breeding technique. Here we report the transmission of such mutations from the callus to regenerants and their progenies, leading to a generation of selectable marker-free (SMF) herbicide tolerant rice plants with simultaneous multiplex nucleotide substitutions. These findings demonstrate that Target-AID can be developed into novel plant breeding technology which enables improvement of multiplex traits at one time in combination with sophisticated targeted base editing with the simplicity and versatility of CRISPR/Cas9 system. |
|---|---|
| Keywords | DNA ; callus ; cytidine deaminase ; gene editing ; herbicide resistance ; inheritance (genetics) ; plant breeding ; point mutation ; rice ; transactivators ; transgenes ; Acetolactate synthase (ALS) ; Activation-induced cytidine deaminase (AID) ; Herbicide tolerance ; Rice ; Selectable marker-free (SMF) ; Target-AID ; Targeted nucleotide editing ; CRISPR/Cas9 ; PAM ; sgRNA ; AID ; HPT ; ALS ; IMZ ; NHEJ ; HR ; SMF |
| Language | English |
| Dates of publication | 2018-10 |
| Size | p. 78-83. |
| Publishing place | Elsevier Masson SAS |
| Document type | Article ; Online |
| ZDB-ID | 742978-2 |
| ISSN | 1873-2690 ; 0981-9428 |
| ISSN (online) | 1873-2690 |
| ISSN | 0981-9428 |
| DOI | 10.1016/j.plaphy.2018.04.028 |
| Database | NAL-Catalogue (AGRICOLA) |
| Z 2696: Show issues | ||||
| Z 2005/727: Show issues |
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PLoS ONE, Vol 7, Iss 11, p e
2012 Volume 50309
Abstract: To analyze an animal's movement trajectory, a basic model is required that satisfies the following conditions: the model must have an ecological basis and the parameters used in the model must have ecological interpretations, a broad range of movement ... ...
| Abstract | To analyze an animal's movement trajectory, a basic model is required that satisfies the following conditions: the model must have an ecological basis and the parameters used in the model must have ecological interpretations, a broad range of movement patterns can be explained by that model, and equations and probability distributions in the model should be mathematically tractable. Random walk models used in previous studies do not necessarily satisfy these requirements, partly because movement trajectories are often more oriented or tortuous than expected from the models. By improving the modeling for turning angles, this study aims to propose a basic movement model. On the basis of the recently developed circular auto-regressive model, we introduced a new movement model and extended its applicability to capture the asymmetric effects of external factors such as wind. The model was applied to GPS trajectories of a seabird (Calonectris leucomelas) to demonstrate its applicability to various movement patterns and to explain how the model parameters are ecologically interpreted under a general conceptual framework for movement ecology. Although it is based on a simple extension of a generalized linear model to circular variables, the proposed model enables us to evaluate the effects of external factors on movement separately from the animal's internal state. For example, maximum likelihood estimates and model selection suggested that in one homing flight section, the seabird intended to fly toward the island, but misjudged its navigation and was driven off-course by strong winds, while in the subsequent flight section, the seabird reset the focal direction, navigated the flight under strong wind conditions, and succeeded in approaching the island. |
|---|---|
| Keywords | Medicine ; R ; Science ; Q |
| Subject code | 612 |
| Language | English |
| Publishing date | 2012-01-01T00:00:00Z |
| Publisher | Public Library of Science (PLoS) |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.