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  1. Article ; Online: Embryo manipulation and imprinting.

    Marchesi, Dennis E / Qiao, Jie / Feng, Huai L

    Seminars in reproductive medicine

    2012  Volume 30, Issue 4, Page(s) 323–334

    Abstract: As the use of assisted reproductive technologies (ART) continues to rise worldwide, it remains of the upmost importance to maintain the safety of those techniques used in ART. Many of these practices are unique to this discipline; as such, it becomes ... ...

    Abstract As the use of assisted reproductive technologies (ART) continues to rise worldwide, it remains of the upmost importance to maintain the safety of those techniques used in ART. Many of these practices are unique to this discipline; as such, it becomes difficult to assess the true risks that the potential offspring may be subjected to under this type of treatment. Removal of oocytes from a woman's body during an in vitro fertilization (IVF) cycle offers an increased opportunity for routine cellular processes to go awry. Specifically, epigenetic modifications and imprinting diseases are rare among the general population; however, although their incidence among IVF-conceived children is also rare, their frequency in this population remains elevated compared with universal rates. Recent investigations have directly attributed their occurrences to the use of ART and IVF to achieve a successful pregnancy. This review discusses the major cellular manipulations of a typical IVF cycle to assess the potential risks versus the reported risks. These manipulations include preimplantation genetic diagnosis and screening, intracytoplasmic sperm injection, ooplasmic transfer, embryo culture, in vitro maturation, and cryopreservation. Oocyte and embryo handling is a delicate part of the IVF process that continues to improve. The safety of those potential improvements is also discussed.
    MeSH term(s) Chromosome Disorders/etiology ; Chromosome Disorders/genetics ; Cryopreservation/methods ; DNA Methylation/physiology ; Embryo Culture Techniques/methods ; Female ; Fertilization in Vitro/methods ; Genomic Imprinting/physiology ; Humans ; Pregnancy ; Preimplantation Diagnosis/methods ; Sperm Injections, Intracytoplasmic/methods
    Language English
    Publishing date 2012-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2042479-6
    ISSN 1526-4564 ; 1526-8004
    ISSN (online) 1526-4564
    ISSN 1526-8004
    DOI 10.1055/s-0032-1320013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Sperm DNA integrity from sperm to egg.

    Marchesi, Dennis E / Feng, Huai L

    Journal of andrology

    2007  Volume 28, Issue 4, Page(s) 481–489

    MeSH term(s) Apoptosis ; Chromatin/genetics ; DNA/genetics ; DNA Damage ; DNA, Mitochondrial/genetics ; Female ; Humans ; Male ; Oxidative Stress ; Sperm-Ovum Interactions/genetics ; Spermatozoa/physiology
    Chemical Substances Chromatin ; DNA, Mitochondrial ; DNA (9007-49-2)
    Language English
    Publishing date 2007-07
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 604624-1
    ISSN 1939-4640 ; 0196-3635
    ISSN (online) 1939-4640
    ISSN 0196-3635
    DOI 10.2164/jandrol.106.002105
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Current assessment of sperm DNA integrity.

    Marchesi, Dennis E / Feng, Huai L / Hershlag, Avner

    Archives of andrology

    2008  Volume 53, Issue 5, Page(s) 239–247

    Abstract: Conventional semen analysis is rapidly losing its place as the gold standard of diagnosis and the cornerstone of treating the infertile male in modern times. Recent technology allows scientists to analyze sperm fertilizing potential and subsequent ... ...

    Abstract Conventional semen analysis is rapidly losing its place as the gold standard of diagnosis and the cornerstone of treating the infertile male in modern times. Recent technology allows scientists to analyze sperm fertilizing potential and subsequent embryonic growth by studying factors that have previously escaped traditional parameters. It has become increasingly evident that nuclear DNA arrangement is essential to the fertilizing potential of sperm. A vast array of tests are now available to examine the genetic makeup of individual spermatozoa, ranging the entire gamut from simple bench top assays performed routinely to complex flow cytometric assays requiring highly-skilled technologists. Future research to compare these new tests to those more commonly in use, correlating them with reproductive outcome promises to fill the current void in the field of male infertility, paring innovative diagnostic (and prognostic) technological standards to the already existing sophisticated assortment of successful treatment modalities.
    MeSH term(s) Chromatin/chemistry ; Comet Assay ; DNA/genetics ; Flow Cytometry ; Humans ; In Situ Nick-End Labeling ; Male ; Spermatozoa/metabolism
    Chemical Substances Chromatin ; DNA (9007-49-2)
    Language English
    Publishing date 2008-02-16
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 430351-9
    ISSN 0148-5016
    ISSN 0148-5016
    DOI 10.1080/01485010701569858
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Embryo Manipulation and Imprinting

    Marchesi, Dennis E. / Qiao, Jie / Feng, Huai L.

    Seminars in Reproductive Medicine

    (Preimplantation Genetic Diagnosis)

    2012  Volume 30, Issue 04, Page(s) 323–334

    Abstract: As the use of assisted reproductive technologies (ART) continues to rise worldwide, it remains of the upmost importance to maintain the safety of those techniques used in ART. Many of these practices are unique to this discipline; as such, it becomes ... ...

    Series title Preimplantation Genetic Diagnosis
    Abstract As the use of assisted reproductive technologies (ART) continues to rise worldwide, it remains of the upmost importance to maintain the safety of those techniques used in ART. Many of these practices are unique to this discipline; as such, it becomes difficult to assess the true risks that the potential offspring may be subjected to under this type of treatment. Removal of oocytes from a woman's body during an in vitro fertilization (IVF) cycle offers an increased opportunity for routine cellular processes to go awry. Specifically, epigenetic modifications and imprinting diseases are rare among the general population; however, although their incidence among IVF-conceived children is also rare, their frequency in this population remains elevated compared with universal rates. Recent investigations have directly attributed their occurrences to the use of ART and IVF to achieve a successful pregnancy. This review discusses the major cellular manipulations of a typical IVF cycle to assess the potential risks versus the reported risks. These manipulations include preimplantation genetic diagnosis and screening, intracytoplasmic sperm injection, ooplasmic transfer, embryo culture, in vitro maturation, and cryopreservation. Oocyte and embryo handling is a delicate part of the IVF process that continues to improve. The safety of those potential improvements is also discussed.
    Keywords PGD/PGS ; ICSI ; oocyte/embryo culture ; IVF safety ; imprinting diseases
    Language English
    Publishing date 2012-06-27
    Publisher Thieme Medical Publishers
    Publishing place Stuttgart ; New York
    Document type Article
    ZDB-ID 2042479-6
    ISSN 1526-4564 ; 1526-8004
    ISSN (online) 1526-4564
    ISSN 1526-8004
    DOI 10.1055/s-0032-1320013
    Database Thieme publisher's database

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  5. Article ; Online: Ultrathin Polydopamine Films with Phospholipid Nanodiscs Containing a Glycophorin A Domain

    Marchesi D'Alvise, Tommaso / Harvey, Sean / Hueske, Lisa / Szelwicka, Jolanta / Veith, Lothar / Knowles, Tuomas P.J. / Kubiczek, Dennis / Flaig, Carolin / Port, Fabian / Gottschalk, Kay E. / Rosenau, Frank / Graczykowski, Bartlomiej / Fytas, George / Ruggeri, Francesco S. / Wunderlich, Katrin / Weil, Tanja

    Advanced Functional Materials

    2020  Volume 30, Issue 21

    Abstract: Cellular membranes have long served as an inspiration for nanomaterial research. The preparation of ultrathin polydopamine (PDA) films with integrated protein pores containing phospholipids and an embedded domain of a membrane protein glycophorin A as ... ...

    Abstract Cellular membranes have long served as an inspiration for nanomaterial research. The preparation of ultrathin polydopamine (PDA) films with integrated protein pores containing phospholipids and an embedded domain of a membrane protein glycophorin A as simplified cell membrane mimics is reported. Large area, ultrathin PDA films are obtained by electropolymerization on gold surfaces with 10–18 nm thickness and dimensions of up to 2.5 cm2. The films are transferred from gold to various other substrates such as nylon mesh, silicon, or substrates containing holes in the micrometer range, and they remain intact even after transfer. The novel transfer technique gives access to freestanding PDA films that remain stable even at the air interfaces with elastic moduli of ≈6–12 GPa, which are higher than any other PDA films reported before. As the PDA film thickness is within the range of cellular membranes, monodisperse protein nanopores, so-called “nanodiscs,” are integrated as functional entities. These nanodisc-containing PDA films can serve as semi-permeable films, in which the embedded pores control material transport. In the future, these simplified cell membrane mimics may offer structural investigations of the embedded membrane proteins to receive an improved understanding of protein-mediated transport processes in cellular membranes.
    Keywords freestanding ultrathin films ; glycophorin A ; membrane mimics ; nanodiscs ; polydopamine
    Subject code 620
    Language English
    Publishing country nl
    Document type Article ; Online
    ZDB-ID 2039420-2
    ISSN 1616-3028 ; 1616-301X
    ISSN (online) 1616-3028
    ISSN 1616-301X
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: The effect of semen processing on sperm DNA integrity: comparison of two techniques using the novel Toluidine Blue Assay.

    Marchesi, Dennis E / Biederman, Hannah / Ferrara, Scott / Hershlag, Avner / Feng, Huai L

    European journal of obstetrics, gynecology, and reproductive biology

    2010  Volume 151, Issue 2, Page(s) 176–180

    Abstract: Objective: The first goal of this study was to determine the effect that semen processing has on sperm DNA integrity. The second goal was to assess which processing technique (modified swim-up versus density gradient centrifugation) results in a ... ...

    Abstract Objective: The first goal of this study was to determine the effect that semen processing has on sperm DNA integrity. The second goal was to assess which processing technique (modified swim-up versus density gradient centrifugation) results in a superior sample. DNA integrity was measured using a novel Toluidine Blue Assay.
    Study design: Side-by-side comparison.
    Materials and methods: Raw semen samples were collected from thirty-two male individuals and scored for routine semen analysis. Prior to discarding the specimens identical aliquots were divided and processed by density gradient centrifugation and a modified swim-up technique. The Toluidine Blue Assay was used to analyze raw and processed samples.
    Results: Both density gradient centrifugation and the modified swim-up improved DNA quality compared to the unprocessed sample. However, the modified swim-up technique proved superior.
    Conclusions: The swim-up technique generates a sperm sample with better DNA integrity. Should DNA integrity correlate with better pregnancy rates in IUI and IVF, respectively, the swim-up may be the sperm processing technique of choice for these procedures.
    MeSH term(s) DNA/analysis ; DNA/physiology ; Fertilization in Vitro/methods ; Humans ; Male ; Semen/chemistry ; Semen/physiology ; Specimen Handling/methods ; Sperm Motility/physiology ; Spermatozoa/chemistry ; Spermatozoa/physiology ; Tolonium Chloride/chemistry
    Chemical Substances Tolonium Chloride (15XUH0X66N) ; DNA (9007-49-2)
    Language English
    Publishing date 2010-08
    Publishing country Ireland
    Document type Comparative Study ; Journal Article
    ZDB-ID 190605-7
    ISSN 1872-7654 ; 0301-2115 ; 0028-2243
    ISSN (online) 1872-7654
    ISSN 0301-2115 ; 0028-2243
    DOI 10.1016/j.ejogrb.2010.05.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Karyomegalic interstitial nephritis and DNA damage-induced polyploidy in Fan1 nuclease-defective knock-in mice.

    Lachaud, Christophe / Slean, Meghan / Marchesi, Francesco / Lock, Claire / Odell, Edward / Castor, Dennis / Toth, Rachel / Rouse, John

    Genes & development

    2016  Volume 30, Issue 6, Page(s) 639–644

    Abstract: The Fan1 endonuclease is required for repair of DNA interstrand cross-links (ICLs). Mutations in human Fan1 cause karyomegalic interstitial nephritis (KIN), but it is unclear whether defective ICL repair is responsible or whether Fan1 nuclease activity ... ...

    Abstract The Fan1 endonuclease is required for repair of DNA interstrand cross-links (ICLs). Mutations in human Fan1 cause karyomegalic interstitial nephritis (KIN), but it is unclear whether defective ICL repair is responsible or whether Fan1 nuclease activity is relevant. We show that Fan1 nuclease-defective (Fan1(nd/nd)) mice develop a mild form of KIN. The karyomegalic nuclei from Fan1(nd/nd) kidneys are polyploid, and fibroblasts from Fan1(nd/nd) mice become polyploid upon ICL induction, suggesting that defective ICL repair causes karyomegaly. Thus, Fan1 nuclease activity promotes ICL repair in a manner that controls ploidy, a role that we show is not shared by the Fanconi anemia pathway or the Slx4-Slx1 nuclease also involved in ICL repair.
    MeSH term(s) Animals ; Cells, Cultured ; DNA Damage/genetics ; DNA Repair/genetics ; Deoxyribonucleases/metabolism ; Endodeoxyribonucleases/genetics ; Endodeoxyribonucleases/metabolism ; Exodeoxyribonucleases ; Gene Knock-In Techniques ; Kidney/pathology ; Mice ; Multifunctional Enzymes ; Nephritis, Interstitial/enzymology ; Nephritis, Interstitial/genetics ; Nephritis, Interstitial/physiopathology ; Polyploidy
    Chemical Substances Multifunctional Enzymes ; Deoxyribonucleases (EC 3.1.-) ; Endodeoxyribonucleases (EC 3.1.-) ; Exodeoxyribonucleases (EC 3.1.-) ; Fan1 protein, mouse (EC 3.1.-)
    Language English
    Publishing date 2016-02-15
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 806684-x
    ISSN 1549-5477 ; 0890-9369
    ISSN (online) 1549-5477
    ISSN 0890-9369
    DOI 10.1101/gad.276287.115
    Database MEDical Literature Analysis and Retrieval System OnLINE

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