Article: The bactericidal action of peroxides; an E.P.R. spin-trapping study.
1994 Volume 21, Issue 3, Page(s) 147–167
Abstract: E.P.R. spin trapping has been employed to study radical production during the bactericidal action ...
| Abstract | E.P.R. spin trapping has been employed to study radical production during the bactericidal action of three peroxide compounds (peracetic acid, 4-percarboxy-N-isobutyltrimellitimide and magnesium monoperoxyphthalate) upon both Gram negative (Escherichia Coli) and Gram positive (Staphylococcus Aureus) bacteria. Use of the spin trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO) has allowed direct detection of both carbon-centred and hydroxyl radicals, which are produced at varying rates for the different bacteria/peracid systems studied. The inhibition of bactericidal action, by DMPO and two antioxidants, Vitamin C and Trolox C, indicates that radicals are the lethal species and evidence is presented which suggests that radical production is internal to the bacterial cell. Hydroxyl radicals are believed to be the lethal species. The effect of added iron chelators and haem protein inhibitors indicates that iron species and haem proteins in particular are involved. A marked variation is found in observed hydroxyl-radical adduct signals with both the nature and concentration of peracid. A strong inverse correlation is found between the concentration of the observed radical adduct signal and the relative strength of the peroxide as a bactericide; use of a stable nitroxide as a radical scavenger confirms that strong bactericides produce radicals at a much faster rate than weak bactericides. Plots of radical generation versus time are correlated with % bacterial kill, offering further evidence that hydroxyl radicals are the lethal species. |
|---|---|
| MeSH term(s) | Artifacts ; Cell Membrane Permeability/drug effects ; Chelating Agents/pharmacology ; Electron Spin Resonance Spectroscopy ; Escherichia coli/drug effects ; Free Radical Scavengers ; Free Radicals ; Metals/metabolism ; Microbial Sensitivity Tests ; Molecular Structure ; Peroxides/pharmacology ; Staphylococcus aureus/drug effects |
| Chemical Substances | Chelating Agents ; Free Radical Scavengers ; Free Radicals ; Metals ; Peroxides |
| Language | English |
| Publishing date | 1994-09 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 1194130-3 |
| ISSN | 1029-2470 ; 1071-5762 |
| ISSN (online) | 1029-2470 |
| ISSN | 1071-5762 |
| DOI | 10.3109/10715769409056566 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 2236: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.