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  1. Article ; Online: Types and Brands of Derived Psychoactive Cannabis Products: An Online Retail Assessment, 2023.

    Rossheim, Matthew E / Tillett, Kayla K / Vasilev, Viktor / LoParco, Cassidy R / Berg, Carla J / Trangenstein, Pamela J / Yockey, R Andrew / Sussman, Steven Y / Siegel, Michael / Jernigan, David H

    Cannabis and cannabinoid research

    2024  

    Abstract: Background: ...

    Abstract Background:
    Language English
    Publishing date 2024-01-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2867624-5
    ISSN 2378-8763 ; 2578-5125
    ISSN (online) 2378-8763
    ISSN 2578-5125
    DOI 10.1089/can.2023.0266
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: The essential but enigmatic regulatory role of HERVH in pluripotency.

    Sexton, Corinne E / Tillett, Richard L / Han, Mira V

    Trends in genetics : TIG

    2021  Volume 38, Issue 1, Page(s) 12–21

    Abstract: Human specific endogenous retrovirus H (HERVH) is highly expressed in both naive and primed ...

    Abstract Human specific endogenous retrovirus H (HERVH) is highly expressed in both naive and primed stem cells and is essential for pluripotency. Despite the proven relationship between HERVH expression and pluripotency, there is no single definitive model for the function of HERVH. Instead, several hypotheses of a regulatory function have been put forward including HERVH acting as enhancers, long noncoding RNAs (lncRNAs), and most recently as markers of topologically associating domain (TAD) boundaries. Recently several enhancer-associated lncRNAs have been characterized, which bind to Mediator and are necessary for promoter-enhancer folding interactions. We propose a synergistic model of HERVH function combining relevant findings and discuss the current limitations for its role in regulation, including the lack of evidence for a pluripotency-associated target gene.
    MeSH term(s) Endogenous Retroviruses/metabolism ; Enhancer Elements, Genetic ; Humans ; RNA, Long Noncoding/metabolism ; Stem Cells/metabolism
    Chemical Substances RNA, Long Noncoding
    Language English
    Publishing date 2021-07-30
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
    ZDB-ID 619240-3
    ISSN 1362-4555 ; 0168-9525 ; 0168-9479
    ISSN (online) 1362-4555
    ISSN 0168-9525 ; 0168-9479
    DOI 10.1016/j.tig.2021.07.007
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Derived psychoactive cannabis products and 4/20 specials: An assessment of popular brands and retail price discounts in Fort Worth, Texas, 2023.

    Rossheim, Matthew E / LoParco, Cassidy R / Berg, Carla J / Tillett, Kayla K / Trangenstein, Pamela J / Henry, Doug / Yockey, R Andrew / Livingston, M Doug / Jernigan, David H / Sussman, Steven Y

    Drug and alcohol dependence

    2024  Volume 256, Page(s) 111119

    Abstract: Background: Retail sales of derived psychoactive cannabis products (DPCPs) have increased in the U.S. since passing the 2018 Farm Bill and is unregulated in most states. This study investigated the types and commonly sold brands of DPCPs as well as ... ...

    Abstract Background: Retail sales of derived psychoactive cannabis products (DPCPs) have increased in the U.S. since passing the 2018 Farm Bill and is unregulated in most states. This study investigated the types and commonly sold brands of DPCPs as well as promotional pricing on April 20th, a day associated with cannabis use.
    Methods: On April 19-20, 2023, investigators conducted telephone surveys with 98 retail stores that sold DPCPs in Fort Worth, Texas (where the market was largely unregulated).
    Results: Delta-8, Delta-9, and Delta-10 THC products were widely available, with 97%, 72%, and 82% of stores selling each type, respectively. Fifteen additional DPCPs were identified, and selling blends containing multiple types of THC was common. Frequently sold brands included Cake, Medusa/Modus, Torch, Urb, Kik, Tyson, 3Chi, Casper, Hidden Hills, Esco Bars, Happi, Hometown Hero, STNR, Bomb Bars, Baked, Hi On Nature, Looper, and Space God. Overall, 45% reported having 4/20 specials discounting prices on DPCPs, smoking devices/accessories, or everything in the store. Several stores also sponsored 4/20 promotional events including free THC gummies and "live delta demos where people can test cartridges and try smoking flower in the store."
    Conclusions: Findings highlight the growing complexity of the DPCP market, including numerous different intoxicating compounds and blends. Policymakers, researchers, and public health professionals should consider these complexities, as well as the commonly sold brands, when developing strategies to regulate DPCPs and protect consumer safety. Pricing policies may be an especially important form of harm reduction during events associated with heavy cannabis use, including 4/20.
    MeSH term(s) Humans ; Cannabis ; Texas ; Hallucinogens ; Marketing ; Commerce ; Analgesics ; Cannabinoid Receptor Agonists
    Chemical Substances Hallucinogens ; Analgesics ; Cannabinoid Receptor Agonists
    Language English
    Publishing date 2024-02-07
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 519918-9
    ISSN 1879-0046 ; 0376-8716
    ISSN (online) 1879-0046
    ISSN 0376-8716
    DOI 10.1016/j.drugalcdep.2024.111119
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Changing medication-related beliefs: A systematic review and meta-analysis of randomized controlled trials.

    Sheils, Elizabeth / Tillett, William / James, Delyth / Brown, Sarah / Dack, Charlotte / Family, Hannah / Chapman, Sarah C E

    Health psychology : official journal of the Division of Health Psychology, American Psychological Association

    2023  Volume 43, Issue 3, Page(s) 155–170

    Abstract: Objective: Medication-related beliefs, for example, beliefs that medicines are unnecessary or that side effects are likely, can influence medication behaviors and experiences, potentially impacting quality of life and mortality. At times, it may be ... ...

    Abstract Objective: Medication-related beliefs, for example, beliefs that medicines are unnecessary or that side effects are likely, can influence medication behaviors and experiences, potentially impacting quality of life and mortality. At times, it may be useful to change medication-related beliefs, for example, to reduce patients' concerns about side effects when extensive evidence suggests side effects are rare. Currently we do not know the most effective methods to address medication beliefs.
    Method: Systematic review and meta-analysis of randomized controlled trials that measured medication-related beliefs in people prescribed medication for long-term condition(s). We extracted data on behavior change techniques (BCTs), belief measure, study and patient characteristics, risk of bias, and quality of description.
    Results: We identified 56 trials randomizing 8,714 participants. In meta-analysis, interventions led to small-to-medium effects (
    Conclusion: It is possible to modify medication-related beliefs using a range of interventions and techniques. Future research should explore the best ways to operationalize these BCTs for specific health conditions to support medication beliefs and improve adherence. (PsycInfo Database Record (c) 2024 APA, all rights reserved).
    MeSH term(s) Humans ; Quality of Life ; Randomized Controlled Trials as Topic ; Behavior Therapy/methods ; Medication Adherence
    Language English
    Publishing date 2023-10-23
    Publishing country United States
    Document type Meta-Analysis ; Systematic Review ; Journal Article
    ZDB-ID 226369-5
    ISSN 1930-7810 ; 0278-6133
    ISSN (online) 1930-7810
    ISSN 0278-6133
    DOI 10.1037/hea0001316
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Alcopops Disproportionately Consumed by Minors in Sexual Assault Cases

    Matthew E. Rossheim / Kwynn M. Gonzalez-Pons / Kayla K. Tillett / Ryan D. Treffers / Pamela J. Trangenstein / Jennifer Lerch / Faye S. Taxman / Kaylin M. Greene / Cassidy R. LoParco / David H. Jernigan

    Health Behavior Research, Vol 6, Iss

    2023  Volume 4

    Abstract: Alcohol is present in a large proportion of sexual assault cases. However, research largely overlooks the role that providing alcoholic beverages – particularly those with high-alcohol- content and/or whose flavors mask the taste of alcohol – may have in ...

    Abstract Alcohol is present in a large proportion of sexual assault cases. However, research largely overlooks the role that providing alcoholic beverages – particularly those with high-alcohol- content and/or whose flavors mask the taste of alcohol – may have in making young people more vulnerable to being assaulted. This research is especially important given the rise in the availability of sugar-sweetened alcopops and their high-alcohol-content counterparts “supersized alcopops,” which contain up to 5.5 standard alcoholic drinks. In the current study, we examined whether alcopops and supersized alcopops, relative to beer, were involved in disproportionately more sexual assault cases involving victims who were minors (< 18 years old) rather than adults. In this secondary data analysis, we used Nexis Uni to search legal documents for the brands of supersized alcopop (Four Loko), alcopop (Smirnoff Ice), and beer (Bud Light) most commonly consumed by underage drinkers. Inclusion criteria were U.S. sexual assault cases occurring from 2010 to 2019 and involving victims who consumed one of these three alcohol brands. Two researchers coded information from the case facts, compared coding, and reaching consensus. Thirty-six cases were included for analyses. Compared to victims of sexual assault who consumed beer, victims who consumed supersized alcopops or alcopops were significantly more likely to be minors. Similar results were observed after adjusting for the victim being given the alcohol by the perpetrator, which was strongly associated with the victim being a minor. This study provides initial evidence that sexual assault perpetrators may disproportionately use alcopops and supersized alcopops for the sexual victimization of minors.
    Keywords keywords: ready-to-drink flavored malt alcoholic beverages ; binge drinking and heavy episodic drinking ; rape ; unlawful forcible intercourse ; sex crimes ; sexual assault ; Special aspects of education ; LC8-6691 ; Public aspects of medicine ; RA1-1270
    Subject code 360
    Language English
    Publishing date 2023-12-01T00:00:00Z
    Publisher New Prairie Press
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Identification and genome sequencing of an influenza H3N2 variant in wastewater from elementary schools during a surge of influenza A cases in Las Vegas, Nevada.

    Vo, Van / Harrington, Anthony / Chang, Ching-Lan / Baker, Hayley / Moshi, Michael A / Ghani, Nabih / Itorralba, Jose Yani / Tillett, Richard L / Dahlmann, Elizabeth / Basazinew, Natnael / Gu, Richard / Familara, Tiffany D / Boss, Sage / Vanderford, Fritz / Ghani, Moonis / Tang, Austin J / Matthews, Alice / Papp, Katerina / Khan, Eakalak /
    Koutras, Carolina / Kan, Horng-Yuan / Lockett, Cassius / Gerrity, Daniel / Oh, Edwin C

    The Science of the total environment

    2023  Volume 872, Page(s) 162058

    Abstract: Real-time surveillance of infectious diseases at schools or in communities is often hampered by delays in reporting due to resource limitations and infrastructure issues. By incorporating quantitative PCR and genome sequencing, wastewater surveillance ... ...

    Abstract Real-time surveillance of infectious diseases at schools or in communities is often hampered by delays in reporting due to resource limitations and infrastructure issues. By incorporating quantitative PCR and genome sequencing, wastewater surveillance has been an effective complement to public health surveillance at the community and building-scale for pathogens such as poliovirus, SARS-CoV-2, and even the monkeypox virus. In this study, we asked whether wastewater surveillance programs at elementary schools could be leveraged to detect RNA from influenza viruses shed in wastewater. We monitored for influenza A and B viral RNA in wastewater from six elementary schools from January to May 2022. Quantitative PCR led to the identification of influenza A viral RNA at three schools, which coincided with the lifting of COVID-19 restrictions and a surge in influenza A infections in Las Vegas, Nevada, USA. We performed genome sequencing of wastewater RNA, leading to the identification of a 2021-2022 vaccine-resistant influenza A (H3N2) 3C.2a1b.2a.2 subclade. We next tested wastewater samples from a treatment plant that serviced the elementary schools, but we were unable to detect the presence of influenza A/B RNA. Together, our results demonstrate the utility of near-source wastewater surveillance for the detection of local influenza transmission in schools, which has the potential to be investigated further with paired school-level influenza incidence data.
    MeSH term(s) Humans ; Influenza, Human/genetics ; Wastewater ; Influenza A Virus, H3N2 Subtype/genetics ; Nevada/epidemiology ; COVID-19/epidemiology ; SARS-CoV-2/genetics ; Wastewater-Based Epidemiological Monitoring ; Influenza Vaccines/genetics ; RNA, Viral ; Schools
    Chemical Substances Wastewater ; Influenza Vaccines ; RNA, Viral
    Language English
    Publishing date 2023-02-08
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2023.162058
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Use of wastewater surveillance for early detection of Alpha and Epsilon SARS-CoV-2 variants of concern and estimation of overall COVID-19 infection burden.

    Vo, Van / Tillett, Richard L / Papp, Katerina / Shen, Shirley / Gu, Richard / Gorzalski, Andrew / Siao, Danielle / Markland, Rayma / Chang, Ching-Lan / Baker, Hayley / Chen, Jingchun / Schiller, Martin / Betancourt, Walter Q / Buttery, Erin / Pandori, Mark / Picker, Michael A / Gerrity, Daniel / Oh, Edwin C

    The Science of the total environment

    2022  Volume 835, Page(s) 155410

    Abstract: A decline in diagnostic testing for SARS-CoV-2 is expected to delay the tracking of COVID-19 variants of concern and interest in the United States. We hypothesize that wastewater surveillance programs provide an effective alternative for detecting ... ...

    Abstract A decline in diagnostic testing for SARS-CoV-2 is expected to delay the tracking of COVID-19 variants of concern and interest in the United States. We hypothesize that wastewater surveillance programs provide an effective alternative for detecting emerging variants and assessing COVID-19 incidence, particularly when clinical surveillance is limited. Here, we analyzed SARS-CoV-2 RNA in wastewater from eight locations across Southern Nevada between March 2020 and April 2021. Trends in SARS-CoV-2 RNA concentrations (ranging from 4.3 log
    MeSH term(s) COVID-19/epidemiology ; Humans ; RNA, Viral ; SARS-CoV-2/genetics ; Seroepidemiologic Studies ; Wastewater ; Wastewater-Based Epidemiological Monitoring
    Chemical Substances RNA, Viral ; Waste Water
    Language English
    Publishing date 2022-04-22
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2022.155410
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Genomic surveillance of Nevada patients revealed prevalence of unique SARS-CoV-2 variants bearing mutations in the RdRp gene.

    Hartley, Paul D / Tillett, Richard L / AuCoin, David P / Sevinsky, Joel R / Xu, Yanji / Gorzalski, Andrew / Pandori, Mark / Buttery, Erin / Hansen, Holly / Picker, Michael A / Rossetto, Cyprian C / Verma, Subhash C

    Journal of genetics and genomics = Yi chuan xue bao

    2021  Volume 48, Issue 1, Page(s) 40–51

    Abstract: Patients with signs of COVID-19 were tested through diagnostic RT-PCR for SARS-CoV-2 using RNA extracted from the nasopharyngeal/nasal swabs. To determine the variants of SARS-CoV-2 circulating in the state of Nevada, specimens from 200 COVID-19 patients ...

    Abstract Patients with signs of COVID-19 were tested through diagnostic RT-PCR for SARS-CoV-2 using RNA extracted from the nasopharyngeal/nasal swabs. To determine the variants of SARS-CoV-2 circulating in the state of Nevada, specimens from 200 COVID-19 patients were sequenced through our robust sequencing platform, which enabled sequencing of SARS-CoV-2 from specimens with even very low viral loads, without the need of culture-based amplification. High genome coverage allowed the identification of single and multi-nucleotide variants in SARS-CoV-2 in the community and their phylogenetic relationships with other variants present during the same period of the outbreak. We report the occurrence of a novel mutation at 323aa (314aa of orf1b) of nsp12 (RNA-dependent RNA polymerase) changed to phenylalanine (F) from proline (P), in the first reported isolate of SARS-CoV-2, Wuhan-Hu-1. This 323F variant was present at a very high frequency in Northern Nevada. Structural modeling determined this mutation in the interface domain, which is important for the association of accessory proteins required for the polymerase. In conclusion, we report the introduction of specific SARS-CoV-2 variants at very high frequency in distinct geographic locations, which is important for understanding the evolution and circulation of SARS-CoV-2 variants of public health importance, while it circulates in humans.
    MeSH term(s) COVID-19/epidemiology ; COVID-19/virology ; Coronavirus RNA-Dependent RNA Polymerase/chemistry ; Coronavirus RNA-Dependent RNA Polymerase/genetics ; Genome, Viral/genetics ; Humans ; Models, Molecular ; Mutation ; Nasopharynx/virology ; Nevada/epidemiology ; Phylogeny ; Prevalence ; RNA, Viral/genetics ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; Spike Glycoprotein, Coronavirus/genetics ; Workflow
    Chemical Substances RNA, Viral ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2 ; Coronavirus RNA-Dependent RNA Polymerase (EC 2.7.7.48) ; NSP12 protein, SARS-CoV-2 (EC 2.7.7.48)
    Language English
    Publishing date 2021-02-18
    Publishing country China
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2374568-X
    ISSN 1873-5533 ; 1673-8527
    ISSN (online) 1873-5533
    ISSN 1673-8527
    DOI 10.1016/j.jgg.2021.01.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Genomic surveillance of Nevada patients revealed prevalence of unique SARS-CoV-2 variants bearing mutations in the RdRp gene.

    Hartley, Paul D / Tillett, Richard L / AuCoin, David P / Sevinsky, Joel R / Xu, Yanji / Gorzalski, Andrew / Pandori, Mark / Buttery, Erin / Hansen, Holly / Picker, Michael A / Rossetto, Cyprian C / Verma, Subhash C

    medRxiv : the preprint server for health sciences

    2020  

    Abstract: Patients with signs of COVID-19 were tested with CDC approved diagnostic RT-PCR for SARS-CoV-2 using RNA extracted from nasopharyngeal/nasal swabs. In order to determine the variants of SARS-CoV-2 circulating in the state of Nevada, 200 patient specimens ...

    Abstract Patients with signs of COVID-19 were tested with CDC approved diagnostic RT-PCR for SARS-CoV-2 using RNA extracted from nasopharyngeal/nasal swabs. In order to determine the variants of SARS-CoV-2 circulating in the state of Nevada, 200 patient specimens from COVID-19 patients were sequenced through our robust protocol for sequencing SARS-CoV-2 genomes. Our protocol enabled sequencing of SARS-CoV-2 genome directly from the specimens, with even very low viral loads, without the need of culture-based amplification. This allowed the identification of specific nucleotide variants including those coding for D614G and clades defining mutations. These sequences were further analyzed for determining SARS-CoV-2 variants circulating in the state of Nevada and their phylogenetic relationships with other variants present in the united states and the world during the same period of the outbreak. Our study reports the occurrence of a novel variant in the nsp12 (RNA dependent RNA Polymerase) protein at residue 323 (314aa of orf1b) to Phenylalanine (F) from Proline (P), present in the original isolate of SARS-CoV-2 (Wuhan-Hu-1). This 323F variant is found at a very high frequency (46% of the tested specimen) in Northern Nevada. Functional significance of this unique and highly prevalent variant of SARS-CoV-2 with RdRp mutation is currently under investigation but structural modeling showed this 323aa residue in the interface domain of RdRp, which is required for association with accessory proteins. In conclusion, we report the introduction of specific SARS-CoV-2 variants at a very high frequency within a distinct geographic location, which is important for clinical and public health perspectives in understanding the evolution of SARS-CoV-2 while in circulation.
    Keywords covid19
    Language English
    Publishing date 2020-10-02
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2020.08.21.20178863
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Most probable numbers of organisms: revised tables for the multiple tube method.

    Tillett, H E

    Epidemiology and infection

    1987  Volume 99, Issue 2, Page(s) 471–476

    Abstract: Estimation of numbers of organisms is often made using dilution series, for example when examining water samples for coliform organisms. In this paper the most probable numbers (MPNs) are calculated for a 15-tube series consisting of five replicates at ... ...

    Abstract Estimation of numbers of organisms is often made using dilution series, for example when examining water samples for coliform organisms. In this paper the most probable numbers (MPNs) are calculated for a 15-tube series consisting of five replicates at three consecutive tenfold dilutions. Exact conditional probabilities are computed to replace previous approximations. When growth is observed in several of the tubes it is not realistic to select a single MPN. Instead a most probable range (MPR) should be reported. But using an MPR creates problems when comparison has to be made with a legislated, single-valued Standard. It is suggested that the wording of the Standards should be expressed differently when the multiple tube method is used.
    MeSH term(s) Bacteria/growth & development ; Bacteriological Techniques ; Probability ; Water Microbiology
    Language English
    Publishing date 1987-10
    Publishing country England
    Document type Journal Article
    ZDB-ID 632982-2
    ISSN 1469-4409 ; 0950-2688
    ISSN (online) 1469-4409
    ISSN 0950-2688
    DOI 10.1017/s0950268800067972
    Database MEDical Literature Analysis and Retrieval System OnLINE

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