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  1. Article ; Online: Growth ability, carbon source utilization and biochemical features of the new specie Zalaria obscura.

    Campana, Raffaella / Palma, Francesco / Sisti, Maurizio

    World journal of microbiology & biotechnology

    2022  Volume 38, Issue 12, Page(s) 229

    Abstract: This research investigated the characteristics of Zalaria obscura LS31012019 in terms of growth ability in different media (SDB, YPD and TSB) and temperatures (22, 25 and 37 °C), utilization of several carbon sources (Glucose, Fructose, Lactose, Sucrose, ...

    Abstract This research investigated the characteristics of Zalaria obscura LS31012019 in terms of growth ability in different media (SDB, YPD and TSB) and temperatures (22, 25 and 37 °C), utilization of several carbon sources (Glucose, Fructose, Lactose, Sucrose, Xylose, Glycerol and Mannitol at 5, 2 and 1%) and several biochemical features (total protein content, Glutathione, pigments), in comparison with those of the phylogenetically related Aureobasidium pullulans ATCC 15233. The best growth of Z. obscura LS31012019 was obtained in YPD at 25 °C with the highest OD value (0.45) after 144 h of incubation, similar to that of A. pullulans ATCC 15233 (0.48). Glucose resulted the preferred carbon source for both the considered yeasts but also sucrose resulted in efficacy supporting the growth of Z. obscura LS31012019 and A. pullulans ATCC 15233, for their ability in converting sucrose to glucose and fructose and the latter into glucose. Interestingly, Z. obscura LS31012019 utilized also glycerol and mannitol. The biochemical analysis showed the similarity of protein profile in Z. obscura LS31012019 and A. pullulans ATCC 15233 (from 90 to 20 kDa) and a reduced GSH content (0.321 and 0.233 µmol/mg). The pigments extraction with hexane generated a yellow oleaginous pellet in both the strains, while a yellow solid matrix more intensely coloured in A. pullulans ATTC 15233 was visible with the following solvent extractions. Overall, our data showed that Z. obscura LS31012019 can grow in different media and temperatures and utilize carbon sources apart from glucose and sucrose, shifting to a non-fermentative metabolism. These results improve the information regarding the characteristics of Z. obscura, opening a new field of investigation for the possible application of new species of black yeasts in human application.
    MeSH term(s) Ascomycota/classification ; Ascomycota/metabolism ; Carbon/metabolism
    Chemical Substances Carbon (7440-44-0)
    Language English
    Publishing date 2022-09-23
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1499109-3
    ISSN 1573-0972 ; 0959-3993
    ISSN (online) 1573-0972
    ISSN 0959-3993
    DOI 10.1007/s11274-022-03417-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Growth ability, carbon source utilization and biochemical features of the new specie Zalaria obscura

    Campana, Raffaella / Palma, Francesco / Sisti, Maurizio

    World J Microbiol Biotechnol. 2022 Dec., v. 38, no. 12, p. 229

    2022  , Page(s) 229

    Abstract: This research investigated the characteristics of Zalaria obscura LS31012019 in terms of growth ability in different media (SDB, YPD and TSB) and temperatures (22, 25 and 37 °C), utilization of several carbon sources (Glucose, Fructose, Lactose, Sucrose, ...

    Abstract This research investigated the characteristics of Zalaria obscura LS31012019 in terms of growth ability in different media (SDB, YPD and TSB) and temperatures (22, 25 and 37 °C), utilization of several carbon sources (Glucose, Fructose, Lactose, Sucrose, Xylose, Glycerol and Mannitol at 5, 2 and 1%) and several biochemical features (total protein content, Glutathione, pigments), in comparison with those of the phylogenetically related Aureobasidium pullulans ATCC 15233. The best growth of Z. obscura LS31012019 was obtained in YPD at 25 °C with the highest OD value (0.45) after 144 h of incubation, similar to that of A. pullulans ATCC 15233 (0.48). Glucose resulted the preferred carbon source for both the considered yeasts but also sucrose resulted in efficacy supporting the growth of Z. obscura LS31012019 and A. pullulans ATCC 15233, for their ability in converting sucrose to glucose and fructose and the latter into glucose. Interestingly, Z. obscura LS31012019 utilized also glycerol and mannitol. The biochemical analysis showed the similarity of protein profile in Z. obscura LS31012019 and A. pullulans ATCC 15233 (from 90 to 20 kDa) and a reduced GSH content (0.321 and 0.233 µmol/mg). The pigments extraction with hexane generated a yellow oleaginous pellet in both the strains, while a yellow solid matrix more intensely coloured in A. pullulans ATTC 15233 was visible with the following solvent extractions. Overall, our data showed that Z. obscura LS31012019 can grow in different media and temperatures and utilize carbon sources apart from glucose and sucrose, shifting to a non-fermentative metabolism. These results improve the information regarding the characteristics of Z. obscura, opening a new field of investigation for the possible application of new species of black yeasts in human application.
    Keywords Aureobasidium pullulans ; carbon ; fructose ; glucose ; glutathione ; glycerol ; hexane ; humans ; lactose ; mannitol ; metabolism ; new species ; phylogeny ; protein content ; solvents ; sucrose ; xylose
    Language English
    Dates of publication 2022-12
    Size p. 229
    Publishing place Springer Netherlands
    Document type Article ; Online
    ZDB-ID 1499109-3
    ISSN 1573-0972 ; 0959-3993
    ISSN (online) 1573-0972
    ISSN 0959-3993
    DOI 10.1007/s11274-022-03417-y
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  3. Article: Intracellular Survival and Translocation Ability of Human and Avian Campylobacter jejuni and Campylobacter coli Strains.

    Campana, Raffaella / Baffone, Wally

    Advances in experimental medicine and biology

    2020  Volume 1282, Page(s) 115–125

    Abstract: Campylobacter acts using complex strategies to establish and promote intestinal infections. After ingestion via contaminated foods, this bacterium invades and can survive within the intestinal cells, also inducing epithelial translocation of non-invasive ...

    Abstract Campylobacter acts using complex strategies to establish and promote intestinal infections. After ingestion via contaminated foods, this bacterium invades and can survive within the intestinal cells, also inducing epithelial translocation of non-invasive intestinal bacteria. In this investigation, the ability of human and avian C. jejuni and C. coli isolates to survive within two different intestinal epithelial cells lines, Caco-2 and INT 407, as well as the intestinal translocation phenomenon, was assessed. Our data demonstrated that both C. jejuni and C. coli strains survived in Caco-2 (81.8% and 100% respectively) and INT 407 monolayers (72.7% and 100% respectively) within the first 24 h post-infection period, with a progressive reduction in the prolonged period of 48 h and 72 h post-infection. The translocation of the non-invasive E. coli 60/06 FB was remarkably increased in C. jejuni treated Caco-2 monolayers (2.36 ± 0.42 log cfu/mL) (P < 0.01) and less in those treated with C. coli (1.2 ± 0.34 log cfu/mL), compared to E. coli 60/06 FB alone (0.37 ± 0.14 log cfu/mL). Our results evidenced the ability of both human and avian strains of C. jejuni and C. coli to efficiently survive within intestinal cells and induce the translocation of a non-invasive pathogen. Overall, these findings stress how this pathogen can interact with host cells and support the hypothesis that defects in the intestinal barrier function induced by Campylobacter spp. could have potentially negative implications for human health.
    MeSH term(s) Animals ; Bacterial Translocation ; Birds/microbiology ; Caco-2 Cells ; Campylobacter Infections ; Campylobacter coli/physiology ; Campylobacter jejuni/physiology ; Cell Line ; Escherichia coli/physiology ; Humans
    Language English
    Publishing date 2020-04-23
    Publishing country United States
    Document type Journal Article
    ISSN 2214-8019 ; 0065-2598
    ISSN (online) 2214-8019
    ISSN 0065-2598
    DOI 10.1007/5584_2020_531
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Efficacy of UV and UV-LEDs Irradiation Models for Microbial Inactivation Applicable to Automated Sterile Drug Compounding.

    Campana, Raffaella / Moroni, Sofia / Paolucci, Demis / Federici, Matteo / Casettari, Luca / Frangipani, Emanuela

    Journal of pharmaceutical sciences

    2023  Volume 112, Issue 9, Page(s) 2389–2392

    Abstract: LEDs development has attracted attention over conventional mercury lamps for the tiny size, high efficiency, long lifetime, low operating temperature. The antimicrobial effectiveness of traditional UV-lamps radiation (wavelength of 254 nm) compared to UV- ...

    Abstract LEDs development has attracted attention over conventional mercury lamps for the tiny size, high efficiency, long lifetime, low operating temperature. The antimicrobial effectiveness of traditional UV-lamps radiation (wavelength of 254 nm) compared to UV-C LEDs (LED1 wavelength range 275-286 nm and LED2 range 260-270 nm) was carried out, for possible applications to automated sterile drug compounding. The UV lamp and the tested UV-LED devices remarkably reduced microbial load, following a time-dose response, but the best performance was evidenced by LED1, which guaranteed the complete inactivation of high concentrations of bacteria, yeasts, and spores at doses between 200 and 2000 J/m
    MeSH term(s) Microbial Viability ; Drug Compounding ; Disinfection ; Ultraviolet Rays ; Bacteria
    Language English
    Publishing date 2023-07-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3151-3
    ISSN 1520-6017 ; 0022-3549
    ISSN (online) 1520-6017
    ISSN 0022-3549
    DOI 10.1016/j.xphs.2023.07.008
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uf I Zs.50: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  5. Article: Synthesis and Biological Evaluation of 6-

    Verboni, Michele / Sisti, Maurizio / Campana, Raffaella / Benedetti, Serena / Palma, Francesco / Potenza, Lucia / Lucarini, Simone / Duranti, Andrea

    Pharmaceuticals (Basel, Switzerland)

    2023  Volume 16, Issue 2

    Abstract: A small library of 6- ...

    Abstract A small library of 6-
    Language English
    Publishing date 2023-01-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2193542-7
    ISSN 1424-8247
    ISSN 1424-8247
    DOI 10.3390/ph16020136
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  6. Article ; Online: 3D printed clotrimazole intravaginal ring for the treatment of recurrent vaginal candidiasis.

    Tiboni, Mattia / Campana, Raffaella / Frangipani, Emanuela / Casettari, Luca

    International journal of pharmaceutics

    2021  Volume 596, Page(s) 120290

    Abstract: Vulvovaginal candidiasis is a vaginal infection caused by the fungal pathogen Candida albicans that, most commonly, affects women of reproductive age. Its first-line treatment consists in topical applications of conventional drug formulations (e.g., ... ...

    Abstract Vulvovaginal candidiasis is a vaginal infection caused by the fungal pathogen Candida albicans that, most commonly, affects women of reproductive age. Its first-line treatment consists in topical applications of conventional drug formulations (e.g., creams, gels, tablets) containing imidazole drugs. The treatment involves single or multiple daily applications and, in the case of recurrences, daily administration of oral antifungal drugs for up to one month. Intravaginal rings are flexible, biocompatible medical devices that, compared to conventional drug formulations, offer the possibility of a controlled vaginal drug delivery over a determined period with a single application, thus increasing patient compliance. Among innovative manufacturing techniques, in recent years, fused deposition modeling 3D printing has emerged in the pharmaceutical field to produce different therapeutics combining drugs and polymers. This technique allows to print objects layer by layer with many different thermoplastic materials after a computer-assisted design. Thermoplastic polyurethanes are flexible and biocompatible materials that can be efficiently employed for the manufacturing of drug release systems, already utilized to prepare vaginal devices. In this work, we produced a clotrimazole-loaded intravaginal ring by fused deposition modeling 3D printing combining the drug with thermoplastic polyurethane using hot melt extrusion. The rings were computer-designed and then printed with two different drug concentrations (i.e., 2% and 10% w/w). The intravaginal rings were first tested in an agar-diffusion test to evaluate their effectiveness against C. albicans; and the 10% loaded ring was selected for further studies. Drug release was evaluated in two different media (i.e., 50% ethanol and vaginal fluid simulant) showing a sustained release over a period of seven days. Next, in vitro time-kill analysis against C. albicans in simulated vaginal fluid was performed and displayed a complete growth inhibition after 5 days, compared to the control. These results suggest a potential application of these 3D printed intravaginal rings for the treatment of vulvovaginal candidiasis and for the long-time treatment of recurrences.
    MeSH term(s) Administration, Intravaginal ; Antifungal Agents/therapeutic use ; Candidiasis, Vulvovaginal/drug therapy ; Clotrimazole ; Drug Liberation ; Female ; Humans ; Printing, Three-Dimensional ; Tablets
    Chemical Substances Antifungal Agents ; Tablets ; Clotrimazole (G07GZ97H65)
    Language English
    Publishing date 2021-01-29
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 428962-6
    ISSN 1873-3476 ; 0378-5173
    ISSN (online) 1873-3476
    ISSN 0378-5173
    DOI 10.1016/j.ijpharm.2021.120290
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1409: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  7. Article: Sugar-Based Monoester Surfactants: Synthetic Methodologies, Properties, and Biological Activities.

    Verboni, Michele / Perinelli, Diego Romano / Buono, Alessandro / Campana, Raffaella / Sisti, Maurizio / Duranti, Andrea / Lucarini, Simone

    Antibiotics (Basel, Switzerland)

    2023  Volume 12, Issue 10

    Abstract: Glycolipids are biocompatible and biodegradable amphiphilic compounds characterized by a great scientific interest for their potential applications in various technological areas, including pharmaceuticals, cosmetics, agriculture, and food production. ... ...

    Abstract Glycolipids are biocompatible and biodegradable amphiphilic compounds characterized by a great scientific interest for their potential applications in various technological areas, including pharmaceuticals, cosmetics, agriculture, and food production. This report summarizes the available synthetic methodologies, physicochemical properties, and biological activity of sugar fatty acid ester surfactants, with a particular focus on 6-
    Language English
    Publishing date 2023-09-30
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2681345-2
    ISSN 2079-6382
    ISSN 2079-6382
    DOI 10.3390/antibiotics12101500
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  8. Article ; Online: A Multidisciplinary Approach in Examining the Susceptibility to Microbial Attack of Polyacrylic and Polyurethane Resins Used in Art Restoration.

    Campana, Raffaella / Sabatini, Luigia / Giorgi, Luca / Pettinari, Giulia / Valentini, Laura / Gobbi, Pietro

    International journal of molecular sciences

    2022  Volume 23, Issue 19

    Abstract: The synthetic polymers used to protect artworks from deterioration process can be colonized by the fungi and bacteria responsible for the biodeterioration process. In this study, the susceptibility of synthetic polyacrylics and polyurethane resins to ... ...

    Abstract The synthetic polymers used to protect artworks from deterioration process can be colonized by the fungi and bacteria responsible for the biodeterioration process. In this study, the susceptibility of synthetic polyacrylics and polyurethane resins to microorganisms (
    MeSH term(s) Bacteria ; Disinfectants ; Esters ; Fungi ; Penicillium ; Polymers ; Polyurethanes/pharmacology
    Chemical Substances Disinfectants ; Esters ; Polymers ; Polyurethanes
    Language English
    Publishing date 2022-10-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms231911725
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  9. Article: Moulds on cementitious building materials—problems, prevention and future perspectives

    Campana, Raffaella / Sabatini, Luigia / Frangipani, Emanuela

    Applied microbiology and biotechnology. 2020 Jan., v. 104, no. 2

    2020  

    Abstract: Materials rich in organic and inorganic compounds, such as building materials or paints, represent an excellent substrate for the development of moulds. Several conditions affect mould’s growth on cementitious materials, such as nutrient and water ... ...

    Abstract Materials rich in organic and inorganic compounds, such as building materials or paints, represent an excellent substrate for the development of moulds. Several conditions affect mould’s growth on cementitious materials, such as nutrient and water availability, temperature, pH and moisture. Microorganisms, and especially moulds, attack these surfaces and contribute to their erosion, thereby reducing the life of the structure itself and negatively affecting human health through inhalation, ingestion and dermal contact with spores. Interventions are based on The European Communities Council Directive 89/106/EEC, that obliges the use of materials, products and building elements that are resistant to fungi and other forms of degradation, and that do not constitute a health risk for users and the environment. This mini-review summarises the current state of problems related to mould growth on cementitious building materials, emphasising new innovative approaches for limiting or contrasting their growth. In particular, the use of nanoparticles and the related nanomaterials as well as the potential use of new “biocides” from natural sources is discussed.
    Keywords construction materials ; dermal exposure ; fungi ; inhalation exposure ; inorganic compounds ; nanoparticles ; oral exposure ; pH ; paints ; risk ; spores ; temperature
    Language English
    Dates of publication 2020-01
    Size p. 509-514.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    Note Review
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-019-10185-7
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    In stock of ZB MED Bonn / Germany

    Z 79/727: Show issues

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  10. Article: Marine Alkaloid 2,2-Bis(6-bromo-3-indolyl) Ethylamine and Its Synthetic Derivatives Inhibit Microbial Biofilms Formation and Disaggregate Developed Biofilms.

    Campana, Raffaella / Favi, Gianfranco / Baffone, Wally / Lucarini, Simone

    Microorganisms

    2019  Volume 7, Issue 2

    Abstract: The antimicrobial activity of the marine bisindole alkaloid 2,2-bis(6-bromo-3-indolyl) ethylamine ( ...

    Abstract The antimicrobial activity of the marine bisindole alkaloid 2,2-bis(6-bromo-3-indolyl) ethylamine (
    Language English
    Publishing date 2019-01-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms7020028
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