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  1. Article: Capillary pruning couples tissue perfusion and oxygenation with cardiomyocyte maturation in the postnatal mouse heart.

    Santamaría, Ricardo / Cruz-Caballero, Javier / Gkontra, Polyxeni / Jiménez-Montiel, Alberto / Clemente, Cristina / López, Juan A / Villalba-Orero, María / Vázquez, Jesús / Hutloff, Andreas / Lara-Pezzi, Enrique / Arroyo, Alicia G

    Frontiers in cell and developmental biology

    2023  Volume 11, Page(s) 1256127

    Abstract: Introduction: ...

    Abstract Introduction:
    Language English
    Publishing date 2023-11-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2737824-X
    ISSN 2296-634X
    ISSN 2296-634X
    DOI 10.3389/fcell.2023.1256127
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Unravelling the orientation of the inositol-biphosphate ring and its dependence on Phosphatidylinositol 4,5-bisphosphate cluster formation in model membranes

    Santamaria, Andreas / Carrascosa-Tejedor, Javier / Guzmán, Eduardo / Zaccai, Nathan R. / Maestro, Armando

    Journal of colloid and interface science. 2022 Sept. 18,

    2022  

    Abstract: Inositol phospholipids are well known to form clusters in the cytoplasmic leaflet of the plasma membrane that are responsible for the interaction and recruitment of proteins involved in key biological processes like endocytosis, ion channel activation ... ...

    Abstract Inositol phospholipids are well known to form clusters in the cytoplasmic leaflet of the plasma membrane that are responsible for the interaction and recruitment of proteins involved in key biological processes like endocytosis, ion channel activation and secondary messenger production. Although their phosphorylated inositol ring headgroup plays an important role in protein binding, its orientation with respect to the plane of the membrane and its lateral packing density has not been previously described experimentally. Here, we study phosphatidylinositol 4,5-bisphosphate (PIP₂) planar model membranes in the form of Langmuir monolayers by surface pressure-area isotherms, Brewster angle microscopy and neutron reflectometry to elucidate the relation between lateral (in-plane) and perpendicular (out-of-plane) molecular organization of PIP₂. Different surface areas were explored through monolayer compression, allowing us to correlate the formation of transient PIP₂ clusters with the change in orientation of the inositol-biphosphate headgroup, which was experimentally determined by neutron reflectometry.
    Keywords endocytosis ; inositols ; ion channels ; microscopy ; phospholipids ; plasma membrane ; reflectometry ; second messengers
    Language English
    Dates of publication 2022-0918
    Publishing place Elsevier Inc.
    Document type Article
    Note Pre-press version
    ZDB-ID 241597-5
    ISSN 1095-7103 ; 0021-9797
    ISSN (online) 1095-7103
    ISSN 0021-9797
    DOI 10.1016/j.jcis.2022.09.095
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  3. Article ; Online: Unravelling the orientation of the inositol-biphosphate ring and its dependence on phosphatidylinositol 4,5-bisphosphate cluster formation in model membranes.

    Santamaria, Andreas / Carrascosa-Tejedor, Javier / Guzmán, Eduardo / Zaccai, Nathan R / Maestro, Armando

    Journal of colloid and interface science

    2022  Volume 629, Issue Pt B, Page(s) 785–795

    Abstract: Hypothesis: Inositol phospholipids are well known to form clusters in the cytoplasmic leaflet of the plasma membrane that are responsible for the interaction and recruitment of proteins involved in key biological processes like endocytosis, ion channel ... ...

    Abstract Hypothesis: Inositol phospholipids are well known to form clusters in the cytoplasmic leaflet of the plasma membrane that are responsible for the interaction and recruitment of proteins involved in key biological processes like endocytosis, ion channel activation and secondary messenger production. Although their phosphorylated inositol ring headgroup plays an important role in protein binding, its orientation with respect to the plane of the membrane and its lateral packing density has not been previously described experimentally.
    Experiments: Here, we study phosphatidylinositol 4,5-bisphosphate (PIP
    Findings: Different surface areas were explored through monolayer compression, allowing us to correlate the formation of transient PIP
    MeSH term(s) Phosphatidylinositols/metabolism ; Phosphatidylinositol 4,5-Diphosphate/chemistry ; Phosphatidylinositol 4,5-Diphosphate/metabolism ; Inositol Phosphates/metabolism ; Cell Membrane/metabolism ; Protein Binding
    Chemical Substances Phosphatidylinositols ; Phosphatidylinositol 4,5-Diphosphate ; Inositol Phosphates
    Language English
    Publishing date 2022-09-23
    Publishing country United States
    Document type Journal Article
    ZDB-ID 241597-5
    ISSN 1095-7103 ; 0021-9797
    ISSN (online) 1095-7103
    ISSN 0021-9797
    DOI 10.1016/j.jcis.2022.09.095
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Structural and functional analysis of the simultaneous binding of two duplex/quadruplex aptamers to human α-thrombin.

    Troisi, Romualdo / Balasco, Nicole / Santamaria, Andreas / Vitagliano, Luigi / Sica, Filomena

    International journal of biological macromolecules

    2021  Volume 181, Page(s) 858–867

    Abstract: The long-range communication between the two exosites of human α-thrombin (thrombin) tightly modulates the protein-effector interactions. Duplex/quadruplex aptamers represent an emerging class of very effective binders of thrombin. Among them, NU172 and ... ...

    Abstract The long-range communication between the two exosites of human α-thrombin (thrombin) tightly modulates the protein-effector interactions. Duplex/quadruplex aptamers represent an emerging class of very effective binders of thrombin. Among them, NU172 and HD22 aptamers are at the forefront of exosite I and II recognition, respectively. The present study investigates the simultaneous binding of these two aptamers by combining a structural and dynamics approach. The crystal structure of the ternary complex formed by the thrombin with NU172 and HD22_27mer provides a detailed view of the simultaneous binding of these aptamers to the protein, inspiring the design of novel bivalent thrombin inhibitors. The crystal structure represents the starting model for molecular dynamics studies, which point out the cooperation between the binding at the two exosites. In particular, the binding of an aptamer to its exosite reduces the intrinsic flexibility of the other exosite, that preferentially assumes conformations similar to those observed in the bound state, suggesting a predisposition to interact with the other aptamer. This behaviour is reflected in a significant increase of the anticoagulant activity of NU172 when the inactive HD22_27mer is bound to exosite II, providing a clear evidence of the synergic action of the two aptamers.
    MeSH term(s) Anticoagulants/pharmacology ; Aptamers, Nucleotide/chemistry ; Aptamers, Nucleotide/metabolism ; Blood Coagulation/drug effects ; Crystallography, X-Ray ; Fibrinogen/metabolism ; Humans ; Molecular Dynamics Simulation ; Protein Binding ; Thrombin/chemistry ; Thrombin/metabolism ; Time Factors
    Chemical Substances Anticoagulants ; Aptamers, Nucleotide ; NU172 ; Fibrinogen (9001-32-5) ; Thrombin (EC 3.4.21.5)
    Language English
    Publishing date 2021-04-14
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2021.04.076
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2374: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Structure of graphene oxide-phospholipid monolayers: A grazing incidence X-ray diffraction and neutron and X-ray reflectivity study.

    Dolores Merchán, M / Pawar, Nisha / Santamaria, Andreas / Sánchez-Fernández, Rosalía / Konovalov, Oleg / Maestro, Armando / Mercedes Velázquez, M

    Journal of colloid and interface science

    2023  Volume 655, Page(s) 664–675

    Abstract: Hypothesis: Graphene oxide-based nanotechnology has aroused a great interest due to its applications in the biomedical and optoelectronic fields. The wide use of these materials makes it necessary to study its potential toxicity associated with the ... ...

    Abstract Hypothesis: Graphene oxide-based nanotechnology has aroused a great interest due to its applications in the biomedical and optoelectronic fields. The wide use of these materials makes it necessary to study its potential toxicity associated with the inhalation of Graphene Oxide (GO) nanoparticles and its interaction with the lung surfactant. Langmuir monolayers have proven to be an excellent tool for studying the properties of the lung surfactant and the effect of intercalation of nanoparticles on its structure and properties. Therefore, to know the origin of the phospholipids/GO interaction and the structure of the lipid layer with GO, in this work we study the effect of the insertion of GO sheets on a Langmuir film of 1,2-Dipalmitoyl-sn-glycerol-3-phosphocholine (DPPC).
    Experiments: Surface pressure-area isotherms, Neutron (NR) and X-ray Reflectivity (XRR) and Grazing Incidence X-ray Diffraction (GIXD) measurements of hydrogenated and deuterated DPPC monolayers with and without GO have been carried out.
    Findings: The results outline a strong interaction between the GO and the zwitterionic form of DPPC and prove that GO is in three regions of the DPPC monolayer, the aliphatic chains of DPPC, the head groups and water in the subphase. Comparison between results obtained with hydrogenated and deuterated DPPC allows concluding that both, electrostatic attractions, and dispersion forces are responsible of the interaction GO/DPPC. Results also demonstrated that the insertion of GO into the DPPC aliphatic chains does not induce significant changes on unit cell of DPPC.
    Language English
    Publishing date 2023-11-10
    Publishing country United States
    Document type Journal Article
    ZDB-ID 241597-5
    ISSN 1095-7103 ; 0021-9797
    ISSN (online) 1095-7103
    ISSN 0021-9797
    DOI 10.1016/j.jcis.2023.11.022
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    In stock of ZB MED Bonn / Germany

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  6. Book ; Online ; Thesis: CA1 pyramidal cells as computational units: From inputs to output

    Yáñez Santamaría, Antonio Benjamín [Verfasser] / Draguhn, Andreas [Akademischer Betreuer]

    2018  

    Author's details Antonio Benjamín Yáñez Santamaría ; Betreuer: Andreas Draguhn
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language English
    Publisher Universitätsbibliothek Heidelberg
    Publishing place Heidelberg
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

    Full text online

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  7. Article ; Online: Inequalities in COVID-19 mortality: defining a global research agenda.

    Friedman, Joseph / Balaj, Mirza / Islam, Nazrul / Gu, Youyang / Nahmia, Petra / Santamaría-Ulloa, Carolina / Gutierrez Rojas, Andres / Rasanathan, Kumanan / Hosseinpoor, Ahmad Reza / Emina, Jacques Bo / Eikemo, Terje Andreas / Castillo-Salgado, Carlos

    Bulletin of the World Health Organization

    2022  Volume 100, Issue 10, Page(s) 648–650

    MeSH term(s) COVID-19 ; Global Health ; Health Status Disparities ; Humans ; Socioeconomic Factors
    Language English
    Publishing date 2022-09-02
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 80213-x
    ISSN 1564-0604 ; 0042-9686 ; 0366-4996 ; 0510-8659
    ISSN (online) 1564-0604
    ISSN 0042-9686 ; 0366-4996 ; 0510-8659
    DOI 10.2471/BLT.22.288211
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 215: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article ; Online: Particle-laden fluid/fluid interfaces: physico-chemical foundations.

    Guzmán, Eduardo / Abelenda-Núñez, Irene / Maestro, Armando / Ortega, Francisco / Santamaria, Andreas / Rubio, Ramón G

    Journal of physics. Condensed matter : an Institute of Physics journal

    2021  Volume 33, Issue 33

    Abstract: Particle-laden fluid/fluid interfaces are ubiquitous in academia and industry, which has fostered extensive research efforts trying to disentangle the physico-chemical bases underlying the trapping of particles to fluid/fluid interfaces as well as the ... ...

    Abstract Particle-laden fluid/fluid interfaces are ubiquitous in academia and industry, which has fostered extensive research efforts trying to disentangle the physico-chemical bases underlying the trapping of particles to fluid/fluid interfaces as well as the properties of the obtained layers. The understanding of such aspects is essential for exploiting the ability of particles on the stabilization of fluid/fluid interface for the fabrication of novel interface-dominated devices, ranging from traditional Pickering emulsions to more advanced reconfigurable devices. This review tries to provide a general perspective of the physico-chemical aspects associated with the stabilization of interfaces by colloidal particles, mainly chemical isotropic spherical colloids. Furthermore, some aspects related to the exploitation of particle-laden fluid/fluid interfaces on the stabilization of emulsions and foams will be also highlighted. It is expected that this review can be used for researchers and technologist as an initial approach to the study of particle-laden fluid layers.
    Language English
    Publishing date 2021-06-29
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1472968-4
    ISSN 1361-648X ; 0953-8984
    ISSN (online) 1361-648X
    ISSN 0953-8984
    DOI 10.1088/1361-648X/ac0938
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Capillary pruning couples tissue perfusion and oxygenation with cardiomyocyte maturation in the postnatal mouse heart

    Ricardo Santamaría / Javier Cruz-Caballero / Polyxeni Gkontra / Alberto Jiménez-Montiel / Cristina Clemente / Juan A. López / María Villalba-Orero / Jesús Vázquez / Andreas Hutloff / Enrique Lara-Pezzi / Alicia G. Arroyo

    Frontiers in Cell and Developmental Biology, Vol

    2023  Volume 11

    Abstract: Introduction: Removal of poorly perfused capillaries by pruning contributes to remodeling the microvasculature to optimize oxygen and nutrient delivery. Blood flow drives this process by promoting the intravascular migration of endothelial cells in ... ...

    Abstract Introduction: Removal of poorly perfused capillaries by pruning contributes to remodeling the microvasculature to optimize oxygen and nutrient delivery. Blood flow drives this process by promoting the intravascular migration of endothelial cells in developing networks, such as in the yolk sac, zebrafish brain or postnatal mouse retina.Methods: In this study, we have implemented innovative tools to recognize capillary pruning in the complex 3D coronary microvasculature of the postnatal mouse heart. We have also experimentally tested the impact of decreasing pruning on the structure and function of this network by altering blood flow with two different vasodilators: losartan and prazosin.Results: Although both drugs reduced capillary pruning, a combination of experiments based on ex vivo imaging, proteomics, electron microscopy and in vivo functional approaches showed that losartan treatment resulted in an inefficient coronary network, reduced myocardial oxygenation and metabolic changes that delayed the arrest of cardiomyocyte proliferation, in contrast to the effects of prazosin, probably due to its concomitant promotion of capillary expansion.Discussion: Our work demonstrates that capillary pruning contributes to proper maturation and function of the heart and that manipulation of blood flow may be a novel strategy to refine the microvasculature and improve tissue perfusion after damage.
    Keywords capillary pruning ; postnatal heart ; vasodilators ; blood flow ; oxygenation ; metabolism ; Biology (General) ; QH301-705.5
    Subject code 610
    Language English
    Publishing date 2023-11-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Polyelectrolyte/surfactant films: from 2D to 3D structural control.

    Carrascosa-Tejedor, Javier / Santamaria, Andreas / Tummino, Andrea / Varga, Imre / Efstratiou, Marina / Lawrence, M Jayne / Maestro, Armando / Campbell, Richard A

    Chemical communications (Cambridge, England)

    2022  Volume 58, Issue 76, Page(s) 10687–10690

    Abstract: Reversible control of the 3D structure of polyelectrolyte/surfactant films at the air/water interface is showcased. A recently discovered mechanism is exploited to form highly efficient, stable and biocompatible films by spreading aggregates composed of ... ...

    Abstract Reversible control of the 3D structure of polyelectrolyte/surfactant films at the air/water interface is showcased. A recently discovered mechanism is exploited to form highly efficient, stable and biocompatible films by spreading aggregates composed of poly-L-lysine and sodium dodecyl sulfate on the surface of water. Reversible control of: (1) the surface monolayer coverage, (2) the switching on or off discrete extended structures, and (3) the extended structure coverage is demonstrated for the first time. The intricacy by which the film structures can be controlled is unprecedented and opens exciting potential to optimize film properties by chemical design for novel biomedical transfer applications.
    MeSH term(s) Excipients ; Polyelectrolytes ; Polylysine ; Sodium Dodecyl Sulfate/chemistry ; Surface Properties ; Surface-Active Agents/chemistry ; Water
    Chemical Substances Excipients ; Polyelectrolytes ; Surface-Active Agents ; Water (059QF0KO0R) ; Polylysine (25104-18-1) ; Sodium Dodecyl Sulfate (368GB5141J)
    Language English
    Publishing date 2022-09-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 1472881-3
    ISSN 1364-548X ; 1359-7345 ; 0009-241X
    ISSN (online) 1364-548X
    ISSN 1359-7345 ; 0009-241X
    DOI 10.1039/d2cc03766a
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