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Article ; Online: Seeing spermine blocking of K+ ion movement through inward rectifier Kir2.2 channels.

Lee, Sun-Joo / Nichols, Colin G

2022 Volume 155, Issue 2

Abstract: Inwardly rectifier potassium (Kir) channels are a major potassium channel sub-class whose function is regulated by ligand-dependent gating and highly voltage-dependent block by polyamines. With molecular dynamics simulations over previously unattainable ... ...

Abstract	Inwardly rectifier potassium (Kir) channels are a major potassium channel sub-class whose function is regulated by ligand-dependent gating and highly voltage-dependent block by polyamines. With molecular dynamics simulations over previously unattainable timescales, Jogini et al. (J. Gen. Physiol. https://doi.org/10.1085/jgp.202213085) provide unprecedented visualization of K+ conduction through open Kir2.2 channels and of the molecular details of channel block by spermine.
MeSH term(s)	Spermine/pharmacology ; Ion Channel Gating ; Polyamines/pharmacology ; Potassium Channel Blockers/pharmacology ; Potassium/pharmacology
Chemical Substances	Spermine (2FZ7Y3VOQX) ; Polyamines ; Potassium Channel Blockers ; Potassium (RWP5GA015D)
Language	English
Publishing date	2022-12-16
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Comment
ZDB-ID	3118-5
ISSN	1540-7748 ; 0022-1295
ISSN (online)	1540-7748
ISSN	0022-1295
DOI	10.1085/jgp.202213144
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Article ; Online: Oxidation Driven Reversal of PIP

Lee, Sun-Joo / Maeda, Shoji / Gao, Jian / Nichols, Colin G

Function (Oxford, England)

2023 Volume 4, Issue 3, Page(s) zqad016

Abstract: Physiological activity of G protein gated inward rectifier ... ...

Abstract	Physiological activity of G protein gated inward rectifier K
MeSH term(s)	G Protein-Coupled Inwardly-Rectifying Potassium Channels/genetics ; GTP-Binding Proteins/metabolism ; Ligands ; Oxidation-Reduction
Chemical Substances	G Protein-Coupled Inwardly-Rectifying Potassium Channels ; GTP-Binding Proteins (EC 3.6.1.-) ; Ligands ; KCNJ6 protein, human
Language	English
Publishing date	2023-04-10
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural
ISSN	2633-8823
ISSN (online)	2633-8823
DOI	10.1093/function/zqad016
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Article ; Online: Development of Fluorescent Bacteria with

Lim, Sun-Joo / Choi, Miae / Yun, Inseop / Lee, Seulgi / Chang, Ny / Lee, Chan-Yong

International journal of molecular sciences

2023 Volume 24, Issue 6

Abstract: Lumazine protein from marine luminescent bacteria ... ...

Abstract	Lumazine protein from marine luminescent bacteria of
MeSH term(s)	Escherichia coli/genetics ; Escherichia coli/metabolism ; Riboflavin/metabolism ; Plasmids/genetics ; Promoter Regions, Genetic ; Operon ; Luminescent Measurements ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism
Chemical Substances	Riboflavin (TLM2976OFR) ; Bacterial Proteins
Language	English
Publishing date	2023-03-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24065096
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Article ; Online: Development of Fluorescent Bacteria with Lux and Riboflavin Genes

Sun-Joo Lim / Miae Choi / Inseop Yun / Seulgi Lee / Ny Chang / Chan-Yong Lee

International Journal of Molecular Sciences, Vol 24, Iss 5096, p

2023 Volume 5096

Abstract: Lumazine protein from marine luminescent bacteria of Photobacterium species bind with very high affinity to the fluorescent chromophore 6,7-dimethyl-8-ribitylumazine. The light emission of bacterial luminescent systems is used as a sensitive, rapid, and ... ...

Abstract	Lumazine protein from marine luminescent bacteria of Photobacterium species bind with very high affinity to the fluorescent chromophore 6,7-dimethyl-8-ribitylumazine. The light emission of bacterial luminescent systems is used as a sensitive, rapid, and safe assay for an ever-increasing number of biological systems. Plasmid pRFN4, containing the genes encoding riboflavin from the rib operon of Bacillus subtilis , was designed for the overproduction of lumazine. To construct fluorescent bacteria for use as microbial sensors, novel recombinant plasmids (pRFN4-Pp N- lum P and pRFN4-Pp lux LP N- lum P) were constructed by amplifying the DNA encoding the N- lum P gene ( lux L) from P. phosphoreum and the promoter region ( lux LP) present upstream of the lux operon of the gene by PCR and ligating into the pRFN4-Pp N- lum P plasmid. A new recombinant plasmid, pRFN4-Pp lux LP-N- lum P, was constructed with the expectation that the fluorescence intensity would be further increased when transformed into Escherichia coli . When this plasmid was transformed into E. coli 43R, the fluorescence intensity of transformants was 500 times greater than that of E. coli alone. As a result, the recombinant plasmid in which the gene encoding N-LumP and DNA containing the lux promoter exhibited expression that was so high as to show fluorescence in single E. coli cells. The fluorescent bacterial systems developed in the present study using lux and riboflavin genes can be utilized in the future as biosensors with high sensitivity and rapid analysis times.
Keywords	bioluminescence ; fluorescence ; lux ; Photobacterium ; riboflavin ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	572
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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Article ; Online: Perceptions of Patient Safety Culture and Medication Error Reporting among Early- and Mid-Career Female Nurses in South Korea

Sun-Joo Jang / Haeyoung Lee / Youn-Jung Son

International Journal of Environmental Research and Public Health, Vol 18, Iss 4853, p

2021 Volume 4853

Abstract: Reporting medication errors is crucial for improving quality of care and patient safety in acute care settings. To date, little is known about how reporting varies between early and mid-career nurses. Thus, this study used a cross-sectional, secondary ... ...

Abstract	Reporting medication errors is crucial for improving quality of care and patient safety in acute care settings. To date, little is known about how reporting varies between early and mid-career nurses. Thus, this study used a cross-sectional, secondary data analysis design to investigate the differences between early (under the age of 35) and mid-career (ages 35–54) female nurses by examining their perceptions of patient safety culture using the Korean Hospital Survey on Patient Safety Culture (HSPSC) and single-item self-report measure of medication error reporting. A total of 311 hospital nurses (260 early-career and 51 mid-career nurses) completed questionnaires on perceived patient safety culture and medication error reporting. Early-career nurses had lower levels of perception regarding patient safety culture ( p = 0.034) compared to mid-career nurses. A multiple logistic regression analysis showed that relatively short clinical experience (<3 years) and a higher level of perceived patient safety culture increased the rate of appropriate medication error reporting among early-career nurses. However, there was no significant association between perception of patient safety culture and medication error reporting among mid-career nurses. Future studies should investigate the role of positive perception of patient safety culture on reporting errors considering multidimensional aspects, and include hospital contextual factors among early-, mid-, and late-career nurses.
Keywords	nurses ; patient safety culture ; medication errors ; expertise ; nursing ; Medicine ; R
Subject code	300
Language	English
Publishing date	2021-05-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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Article ; Online: Perceptions of Patient Safety Culture and Medication Error Reporting among Early- and Mid-Career Female Nurses in South Korea.

Jang, Sun-Joo / Lee, Haeyoung / Son, Youn-Jung

International journal of environmental research and public health

2021 Volume 18, Issue 9

Abstract	Reporting medication errors is crucial for improving quality of care and patient safety in acute care settings. To date, little is known about how reporting varies between early and mid-career nurses. Thus, this study used a cross-sectional, secondary data analysis design to investigate the differences between early (under the age of 35) and mid-career (ages 35-54) female nurses by examining their perceptions of patient safety culture using the Korean Hospital Survey on Patient Safety Culture (HSPSC) and single-item self-report measure of medication error reporting. A total of 311 hospital nurses (260 early-career and 51 mid-career nurses) completed questionnaires on perceived patient safety culture and medication error reporting. Early-career nurses had lower levels of perception regarding patient safety culture (
MeSH term(s)	Adult ; Cross-Sectional Studies ; Female ; Humans ; Medication Errors ; Middle Aged ; Nurses ; Nursing Staff, Hospital ; Organizational Culture ; Patient Safety ; Perception ; Republic of Korea ; Safety Management ; Surveys and Questionnaires
Language	English
Publishing date	2021-05-01
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2175195-X
ISSN	1660-4601 ; 1661-7827
ISSN (online)	1660-4601
ISSN	1661-7827
DOI	10.3390/ijerph18094853
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Article ; Online: Polyamines and potassium channels: A 25-year romance.

Nichols, Colin G / Lee, Sun-Joo

The Journal of biological chemistry

2018 Volume 293, Issue 48, Page(s) 18779–18788

Abstract: Potassium channels that exhibit the property of inward rectification (Kir channels) are present in most cells. Cloning of the first Kir channel genes 25 years ago led to recognition that inward rectification is a consequence of voltage-dependent block by ...

Abstract	Potassium channels that exhibit the property of inward rectification (Kir channels) are present in most cells. Cloning of the first Kir channel genes 25 years ago led to recognition that inward rectification is a consequence of voltage-dependent block by cytoplasmic polyamines, which are also ubiquitously present in animal cells. Upon cellular depolarization, these polycationic metabolites enter the Kir channel pore from the intracellular side, blocking the movement of K
MeSH term(s)	Ion Transport ; Polyamines/metabolism ; Potassium/metabolism ; Potassium Channels/chemistry ; Potassium Channels/genetics ; Potassium Channels/metabolism ; Protein Conformation
Chemical Substances	Polyamines ; Potassium Channels ; Potassium (RWP5GA015D)
Language	English
Publishing date	2018-10-17
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	2997-x
ISSN	1083-351X ; 0021-9258
ISSN (online)	1083-351X
ISSN	0021-9258
DOI	10.1074/jbc.TM118.003344
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Article ; Online: Subunit gating resulting from individual protonation events in Kir2 channels.

Maksaev, Grigory / Bründl-Jirout, Michael / Stary-Weinzinger, Anna / Zangerl-Plessl, Eva-Maria / Lee, Sun-Joo / Nichols, Colin G

Nature communications

2023 Volume 14, Issue 1, Page(s) 4538

Abstract: Inwardly rectifying potassium (Kir) channels open at the 'helix bundle crossing' (HBC), formed by the M2 helices at the cytoplasmic end of the transmembrane pore. Introduced negative charges at the HBC (G178D) in Kir2.2 channels forces opening, allowing ... ...

Abstract	Inwardly rectifying potassium (Kir) channels open at the 'helix bundle crossing' (HBC), formed by the M2 helices at the cytoplasmic end of the transmembrane pore. Introduced negative charges at the HBC (G178D) in Kir2.2 channels forces opening, allowing pore wetting and free movement of permeant ions between the cytoplasm and the inner cavity. Single-channel recordings reveal striking, pH-dependent, subconductance behaviors in G178D (or G178E and equivalent Kir2.1[G177E]) mutant channels, with well-resolved non-cooperative subconductance levels. Decreasing cytoplasmic pH shifts the probability towards lower conductance levels. Molecular dynamics simulations show how protonation of Kir2.2[G178D], or the D173 pore-lining residues, changes solvation, K
MeSH term(s)	Ions ; Molecular Dynamics Simulation ; Cytoplasm ; Oocytes
Chemical Substances	Ions
Language	English
Publishing date	2023-07-28
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-023-40058-7
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Article: Subunit gating resulting from individual protonation events in Kir2 channels.

Maksaev, Grigory / Bründl-Jirout, Michael / Stary-Weinzinger, Anna / Zangerl-Plessl, Eva-Maria / Lee, Sun-Joo / Nichols, Colin G

Research square

2023

Abstract: Inwardly rectifying potassium (Kir) channels play a critical role in stabilizing the membrane potential, thus controlling numerous physiological phenomena in multiple tissues. Channel conductance is activated by cytoplasmic modulators that open the ... ...

Abstract	Inwardly rectifying potassium (Kir) channels play a critical role in stabilizing the membrane potential, thus controlling numerous physiological phenomena in multiple tissues. Channel conductance is activated by cytoplasmic modulators that open the channel at the 'helix bundle crossing' (HBC), formed by the coming together of the M2 helices from each of the four subunits, at the cytoplasmic end of the transmembrane pore. We introduced a negative charge at the bundle crossing region (G178D) in classical inward rectifier Kir2.2 channel subunits that forces channel opening, allowing pore wetting and free movement of permeant ions between the cytoplasm and the inner cavity. Single-channel recordings reveal a striking pH-dependent subconductance behavior in G178D (or G178E and equivalent Kir2.1[G177E]) mutant channels that reflects individual subunit events. These subconductance levels are well resolved temporally and occur independently, with no evidence of cooperativity. Decreasing cytoplasmic pH shifts the probability towards lower conductance levels, and molecular dynamics simulations show how protonation of Kir2.2[G178D] and, additionally, the rectification controller (D173) pore-lining residues leads to changes in pore solvation, K+ ion occupancy, and ultimately K+ conductance. While subconductance gating has long been discussed, resolution and explanation have been lacking. The present data reveals how individual protonation events change the electrostatic microenvironment of the pore, resulting in distinct, uncoordinated, and relatively long-lasting conductance states, which depend on levels of ion pooling in the pore and the maintenance of pore wetting. Gating and conductance are classically understood as separate processes in ion channels. The remarkable sub-state gating behavior of these channels reveals how intimately connected 'gating' and 'conductance' are in reality.
Language	English
Publishing date	2023-03-16
Publishing country	United States
Document type	Preprint
DOI	10.21203/rs.3.rs-2640647/v1
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Article ; Online: Timing of hormone therapy and its association with cardiovascular risk and metabolic parameters in 4-vinylcyclohexene diepoxide-induced primary ovarian insufficiency mouse model.

Lee, Hyun Joo / Park, Min Jung / Heo, Jeong-Doo / Joo, Bo Sun / Joo, Jong Kil

Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology

2023 Volume 39, Issue 1, Page(s) 2247094

Abstract: Objective: To evaluate the effects of various initiation time points and durations of hormone therapy (HT) on cardiovascular and metabolic parameters of premenarche, primary ovarian insufficiency (POI) mouse model, induced by 4-vinylcyclohexene ... ...

Abstract	Objective: To evaluate the effects of various initiation time points and durations of hormone therapy (HT) on cardiovascular and metabolic parameters of premenarche, primary ovarian insufficiency (POI) mouse model, induced by 4-vinylcyclohexene diepoxide. Methods: A total of 50 mice at 4 weeks of age were developed into POI mouse model, further randomly categorized into 5 groups: control group without any intervention; no HT group with only high-fat diet (NT); group 1 with delayed estradiol treatment (T1); group 2 with on-time, continuous estradiol treatment (T2); and group 3 with on-time estradiol treatment but early stop (T3). Cardiovascular risk and metabolic parameters were measured. Results: Presenting with similar body weights, blood glucose levels of T1, T2, and T3 were all significantly lower than NT ( Conclusions: On-time, continuous E2 treatment immediately after a biologic estrogen deprivation event significantly reduced metabolic and cardiovascular risks in young, pre-menarche female mouse models of POI, confirming decreased serum levels of pro-inflammatory cytokines.
MeSH term(s)	Female ; Animals ; Mice ; Humans ; Cardiovascular Diseases/etiology ; Primary Ovarian Insufficiency/chemically induced ; Risk Factors ; Heart Disease Risk Factors ; Cytokines ; Disease Models, Animal ; Estradiol ; Cholesterol
Chemical Substances	4-vinyl-1-cyclohexene dioxide (596C064IG4) ; Cytokines ; Estradiol (4TI98Z838E) ; Cholesterol (97C5T2UQ7J)
Language	English
Publishing date	2023-08-02
Publishing country	England
Document type	Journal Article
ZDB-ID	639237-4
ISSN	1473-0766 ; 0951-3590
ISSN (online)	1473-0766
ISSN	0951-3590
DOI	10.1080/09513590.2023.2247094
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