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  1. Article: Current and Future Advances in the Detection and Surveillance of Biosecurity-Relevant Equine Bacterial Diseases Using Loop-Mediated Isothermal Amplification (LAMP).

    Knox, Alexandra / Zerna, Gemma / Beddoe, Travis

    Animals : an open access journal from MDPI

    2023  Volume 13, Issue 16

    Abstract: Horses play an important role throughout the world, whether for work, culture, or leisure, providing an ever-growing significant contribution to the economy. The increase in importation and movement of horses, both nationally and internationally, has ... ...

    Abstract Horses play an important role throughout the world, whether for work, culture, or leisure, providing an ever-growing significant contribution to the economy. The increase in importation and movement of horses, both nationally and internationally, has inevitably allowed for the global equine industry to grow. Subsequently, however, the potential for transmission of fatal equine bacterial diseases has also escalated, and devasting outbreaks continue to occur. To prevent such events, disease surveillance and diagnosis must be heightened throughout the industry. Current common, or "gold-standard" techniques, have shown to be inadequate at times, thus requiring newer technology to impede outbreaks. Loop-mediated isothermal amplification (LAMP) has proven to be a reliable, rapid, and accessible tool in both diagnostics and surveillance. This review will discuss equine bacterial diseases of biosecurity relevance and their current diagnostic approaches, as well as their respective LAMP assay developments. Additionally, we will provide insight regarding newer technology and advancements associated with this technique and their potential use for the outlined diseases.
    Language English
    Publishing date 2023-08-18
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani13162663
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Environmental detection of

    Tran, Lily / Toet, Hayley / Beddoe, Travis

    PeerJ

    2022  Volume 10, Page(s) e13778

    Abstract: ... Fasciola ... ...

    Abstract Fasciola hepatica
    Language English
    Publishing date 2022-08-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2703241-3
    ISSN 2167-8359
    ISSN 2167-8359
    DOI 10.7717/peerj.13778
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Isothermal Nucleic Acid Amplification Technologies for the Detection of Equine Viral Pathogens.

    Knox, Alexandra / Beddoe, Travis

    Animals : an open access journal from MDPI

    2021  Volume 11, Issue 7

    Abstract: The global equine industry provides significant economic contributions worldwide, producing approximately USD $300 billion annually. However, with the continuous national and international movement and importation of horses, there is an ongoing threat of ...

    Abstract The global equine industry provides significant economic contributions worldwide, producing approximately USD $300 billion annually. However, with the continuous national and international movement and importation of horses, there is an ongoing threat of a viral outbreak causing large epidemics and subsequent significant economic losses. Additionally, horses serve as a host for several zoonotic diseases that could cause significant human health problems. The ability to rapidly diagnose equine viral diseases early could lead to better management, treatment, and biosecurity strategies. Current serological and molecular methods cannot be field-deployable and are not suitable for resource-poor laboratories due to the requirement of expensive equipment and trained personnel. Recently, isothermal nucleic acid amplification technologies, such as loop-mediated isothermal amplification (LAMP) and insulated isothermal polymerase chain reaction (iiPCR), have been developed to be utilized in-field, and provide rapid results within an hour. We will review current isothermal diagnostic techniques available to diagnose equine viruses of biosecurity and zoonotic concern and provide insight into their potential for in-field deployment.
    Language English
    Publishing date 2021-07-20
    Publishing country Switzerland
    Document type Journal Article ; Review
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani11072150
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Development of molecular detection methods of Bovicola ovis from sheep fleece.

    Tran, Lily / Rawlin, Grant T / Beddoe, Travis

    Parasitology research

    2022  Volume 121, Issue 6, Page(s) 1597–1606

    Abstract: The sheep body louse (Bovicola ovis) commonly referred to as sheep lice are small chewing ectoparasites of sheep. Infection results in significant economic costs to the Australian sheep industry due to reduced wool quality caused by chronic itching from ... ...

    Abstract The sheep body louse (Bovicola ovis) commonly referred to as sheep lice are small chewing ectoparasites of sheep. Infection results in significant economic costs to the Australian sheep industry due to reduced wool quality caused by chronic itching from sheep rubbing and biting fleece. Treatment relies on use of insecticides; however, resistance has developed against pyrethroid and other insect growth regulator lousicides. There is urgent need to develop cost-effective lice management to reduce the use of insecticides, with the application of insecticidal treatments only applied when an infestation is detected. However, the current detection method relies on fleece parting for detection of B. ovis which is highly dependent on the skill of the inspector, the number of sheep examined, and the prevalence and severity of the infestation. To improve B. ovis detection, a highly sensitive (5 × 10
    MeSH term(s) Animals ; Australia ; Insecticides ; Ischnocera ; Lice Infestations/diagnosis ; Lice Infestations/epidemiology ; Lice Infestations/veterinary ; Phthiraptera ; Sheep ; Sheep Diseases/parasitology ; Wool/parasitology
    Chemical Substances Insecticides
    Language English
    Publishing date 2022-04-18
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 284966-5
    ISSN 1432-1955 ; 0932-0113 ; 0044-3255
    ISSN (online) 1432-1955
    ISSN 0932-0113 ; 0044-3255
    DOI 10.1007/s00436-022-07520-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Isothermal Nucleic Acid Amplification Technologies for the Detection of Equine Viral Pathogens

    Alexandra Knox / Travis Beddoe

    Animals, Vol 11, Iss 2150, p

    2021  Volume 2150

    Abstract: The global equine industry provides significant economic contributions worldwide, producing approximately USD $300 billion annually. However, with the continuous national and international movement and importation of horses, there is an ongoing threat of ...

    Abstract The global equine industry provides significant economic contributions worldwide, producing approximately USD $300 billion annually. However, with the continuous national and international movement and importation of horses, there is an ongoing threat of a viral outbreak causing large epidemics and subsequent significant economic losses. Additionally, horses serve as a host for several zoonotic diseases that could cause significant human health problems. The ability to rapidly diagnose equine viral diseases early could lead to better management, treatment, and biosecurity strategies. Current serological and molecular methods cannot be field-deployable and are not suitable for resource-poor laboratories due to the requirement of expensive equipment and trained personnel. Recently, isothermal nucleic acid amplification technologies, such as loop-mediated isothermal amplification (LAMP) and insulated isothermal polymerase chain reaction (iiPCR), have been developed to be utilized in-field, and provide rapid results within an hour. We will review current isothermal diagnostic techniques available to diagnose equine viruses of biosecurity and zoonotic concern and provide insight into their potential for in-field deployment.
    Keywords equine ; viruses ; loop-mediated isothermal amplification ; insulated isothermal polymerase chain reaction ; field-deployable ; point-of-care testing ; Veterinary medicine ; SF600-1100 ; Zoology ; QL1-991
    Subject code 630
    Language English
    Publishing date 2021-07-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: Current Status for Controlling the Overlooked Caprine Fasciolosis.

    Zerna, Gemma / Spithill, Terry W / Beddoe, Travis

    Animals : an open access journal from MDPI

    2021  Volume 11, Issue 6

    Abstract: The disease fasciolosis is caused by the liver ... ...

    Abstract The disease fasciolosis is caused by the liver flukes
    Language English
    Publishing date 2021-06-18
    Publishing country Switzerland
    Document type Journal Article ; Review
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani11061819
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Current and Future Advances in the Detection and Surveillance of Biosecurity-Relevant Equine Bacterial Diseases Using Loop-Mediated Isothermal Amplification (LAMP)

    Knox, Alexandra / Zerna, Gemma / Beddoe, Travis

    Animals. 2023 Aug. 18, v. 13, no. 16

    2023  

    Abstract: Horses play an important role throughout the world, whether for work, culture, or leisure, providing an ever-growing significant contribution to the economy. The increase in importation and movement of horses, both nationally and internationally, has ... ...

    Abstract Horses play an important role throughout the world, whether for work, culture, or leisure, providing an ever-growing significant contribution to the economy. The increase in importation and movement of horses, both nationally and internationally, has inevitably allowed for the global equine industry to grow. Subsequently, however, the potential for transmission of fatal equine bacterial diseases has also escalated, and devasting outbreaks continue to occur. To prevent such events, disease surveillance and diagnosis must be heightened throughout the industry. Current common, or “gold-standard” techniques, have shown to be inadequate at times, thus requiring newer technology to impede outbreaks. Loop-mediated isothermal amplification (LAMP) has proven to be a reliable, rapid, and accessible tool in both diagnostics and surveillance. This review will discuss equine bacterial diseases of biosecurity relevance and their current diagnostic approaches, as well as their respective LAMP assay developments. Additionally, we will provide insight regarding newer technology and advancements associated with this technique and their potential use for the outlined diseases.
    Keywords biosecurity ; diagnostic techniques ; disease surveillance ; horses ; industry ; loop-mediated isothermal amplification ; monitoring
    Language English
    Dates of publication 2023-0818
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani13162663
    Database NAL-Catalogue (AGRICOLA)

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  8. Article: Current Status of Loop-Mediated Isothermal Amplification Technologies for the Detection of Honey Bee Pathogens.

    Cameron, Timothy C / Wiles, Danielle / Beddoe, Travis

    Frontiers in veterinary science

    2021  Volume 8, Page(s) 659683

    Abstract: Approximately one-third of the typical human Western diet depends upon pollination for production, and honey bees ( ...

    Abstract Approximately one-third of the typical human Western diet depends upon pollination for production, and honey bees (
    Language English
    Publishing date 2021-04-12
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2834243-4
    ISSN 2297-1769
    ISSN 2297-1769
    DOI 10.3389/fvets.2021.659683
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Environmental detection of Fasciola hepatica by loop-mediated isothermal amplification

    Lily Tran / Hayley Toet / Travis Beddoe

    PeerJ, Vol 10, p e

    2022  Volume 13778

    Abstract: Fasciola hepatica, commonly referred to as liver flukes, is a substantial zoonotic parasitic disease of humans and livestock globally. While infection is readily controlled by anthelmintics, namely triclabendazole, the heavy reliance on triclabendazole ... ...

    Abstract Fasciola hepatica, commonly referred to as liver flukes, is a substantial zoonotic parasitic disease of humans and livestock globally. While infection is readily controlled by anthelmintics, namely triclabendazole, the heavy reliance on triclabendazole has resulted in drug resistance appearing worldwide. Due to drug resistance, it is imperative to adopt an integrated parasite management program to preserve the efficacy of currently available anthelmintics. A integrated liver fluke management plan would benefit from a simple rapid, field-deployable diagnostic for detection of F. hepatica in environment and the host. Therefore, a rapid DNA test using loop-mediated isothermal amplification was developed and optimised for the detection of F. hepatica from faecal and water samples to enable the detection of parasites both within the host and from the environment. The assay presented here is fast, with amplification in ≤20 min, and highly sensitive, with a detection limit of 5 × 10−4 ng/µL. The workflow presented here provides a time to result of ≤60 min without requiring a commercial kit for the extraction of DNA from faecal and water samples, and pending further validation from field-samples, could potentially be used to enable real-time decision making to mitigate parasite prevalence on a farming property and with no requirement for sample transportation.
    Keywords Fasciola hepatica ; LAMP ; Environment ; Fluke ; Parasite ; Field ; Medicine ; R ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2022-08-01T00:00:00Z
    Publisher PeerJ Inc.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article: Characterization of a profilin-like protein from

    Wilkie, Jessica / Cameron, Timothy C / Beddoe, Travis

    PeerJ

    2020  Volume 8, Page(s) e10503

    Abstract: ... Fasciola ... ...

    Abstract Fasciola hepatica
    Language English
    Publishing date 2020-12-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2703241-3
    ISSN 2167-8359
    ISSN 2167-8359
    DOI 10.7717/peerj.10503
    Database MEDical Literature Analysis and Retrieval System OnLINE

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