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Article ; Online: 4-Deoxy-l-

Kawai, Shigeyuki / Hashimoto, Wataru

2022 Volume 27, Issue 2

Abstract: 4-Deoxy-l- ...

Abstract	4-Deoxy-l-
MeSH term(s)	Alginates/metabolism ; Hexuronic Acids/metabolism ; Oxidoreductases/metabolism
Chemical Substances	Alginates ; Hexuronic Acids ; Oxidoreductases (EC 1.-)
Language	English
Publishing date	2022-01-06
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules27020338
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Article ; Online: 4-Deoxy- l - erythro -5-hexoseulose Uronate (DEH) and DEH Reductase

Shigeyuki Kawai / Wataru Hashimoto

Molecules, Vol 27, Iss 338, p

Key Molecule and Enzyme for the Metabolism and Utilization of Alginate

2022 Volume 338

Abstract: 4-Deoxy- l - erythro -5-hexoseulose uronate (DEH), DEH reductase, and alginate lyase have key roles in the metabolism of alginate, a promising carbon source in brown macroalgae for biorefinery. In contrast to the widely reviewed alginate lyase, DEH and ... ...

Abstract	4-Deoxy- l - erythro -5-hexoseulose uronate (DEH), DEH reductase, and alginate lyase have key roles in the metabolism of alginate, a promising carbon source in brown macroalgae for biorefinery. In contrast to the widely reviewed alginate lyase, DEH and DEH reductase have not been previously reviewed. Here, we summarize the current understanding of DEH and DEH reductase, with emphasis on (i) the non-enzymatic and enzymatic formation and structure of DEH and its reactivity to specific amino groups, (ii) the molecular identification, classification, function, and structure, as well as the structural determinants for coenzyme specificity of DEH reductase, and (iii) the significance of DEH for biorefinery. Improved understanding of this and related fields should lead to the practical utilization of alginate for biorefinery.
Keywords	alginate ; 4,5-unsaturated uronates ; 4-deoxy- l - erythro -5-hexoseulose uronate (DEH) ; Sphingomonas sp. A1 ; DEH reductase ; SDR superfamily ; Organic chemistry ; QD241-441
Subject code	540
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Bacteria with a mouth: Discovery and new insights into cell surface structure and macromolecule transport.

Murata, Kousaku / Kawai, Shigeyuki / Hashimoto, Wataru

Proceedings of the Japan Academy. Series B, Physical and biological sciences

2022 Volume 98, Issue 10, Page(s) 529–552

Abstract: A bacterium with a "mouth"-like pit structure isolated for the first time in the history of microbiology was a Gram-negative rod, containing glycosphingolipids in the cell envelope, and named Sphingomonas sp. strain A1. The pit was dynamic, with ... ...

Abstract	A bacterium with a "mouth"-like pit structure isolated for the first time in the history of microbiology was a Gram-negative rod, containing glycosphingolipids in the cell envelope, and named Sphingomonas sp. strain A1. The pit was dynamic, with repetitive opening and closing during growth on alginate, and directly included alginate concentrated around the pit, particularly by flagellins, an alginate-binding protein localized on the cell surface. Alginate incorporated into the periplasm was subsequently transferred to the cytoplasm by cooperative interactions of periplasmic solute-binding proteins and an ATP-binding cassette transporter in the cytoplasmic membrane. The mechanisms of assembly, functions, and interactions between the above-mentioned molecules were clarified using structural biology. The pit was transplanted into other strains of sphingomonads, and the pitted recombinant cells were effectively applied to the production of bioethanol, bioremediation for dioxin removal, and other tasks. Studies of the function of the pit shed light on the biological significance of cell surface structures and macromolecule transport in bacteria.
Language	English
Publishing date	2022-12-12
Publishing country	Japan
Document type	Journal Article
ZDB-ID	161781-3
ISSN	1349-2896 ; 0386-2208
ISSN (online)	1349-2896
ISSN	0386-2208
DOI	10.2183/pjab.98.027
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Article ; Online: Nasal-subcutaneous prime-boost regimen for inactivated whole-virus influenza vaccine efficiently protects mice against both upper and lower respiratory tract infections.

Shibuya, Meito / Tamiya, Shigeyuki / Kawai, Atsushi / Yoshioka, Yasuo

Biochemical and biophysical research communications

2021 Volume 554, Page(s) 166–172

Abstract: Although influenza vaccines are effective for reducing viral transmission and the severity of clinical symptoms, influenza viruses still induce considerable morbidity and mortality worldwide. Seasonal influenza viruses infect the upper respiratory tract ... ...

Abstract	Although influenza vaccines are effective for reducing viral transmission and the severity of clinical symptoms, influenza viruses still induce considerable morbidity and mortality worldwide. Seasonal influenza viruses infect the upper respiratory tract initially but then often induce severe pulmonary complications in the lower respiratory tract. Therefore, influenza vaccines that prevent viral infection at both the upper and lower respiratory tracts are highly anticipated. Here, we examined whether using different vaccination routes for priming and boosting achieved protection in both regions of the respiratory tract. To this end, we used inactivated whole-virion influenza vaccines to immunize mice either subcutaneously or intranasally for both priming and boosting. Regardless of the route used for boosting, the levels of virus-specific IgG in plasma were higher in mice primed subcutaneously than those in control mice, which received PBS only. In addition, intranasal priming followed by subcutaneous boosting induced higher levels of virus-specific IgG in plasma than those in control mice. The levels of virus-specific nasal IgA were higher in mice that were primed intranasally than in control mice or in mice primed subcutaneously. Furthermore, intranasal priming but not subcutaneous priming provided protection against viral challenge in the upper respiratory tract. In addition, when coupled with subcutaneous boosting, both subcutaneous and intranasal priming protected against viral challenge in the lower respiratory tract. These results indicate that intranasal priming followed by subcutaneous boosting induces both virus-specific IgG in plasma and IgA in nasal washes and protects against virus challenge in both the upper and lower respiratory tracts. Our results will help to develop novel vaccines against influenza viruses and other respiratory viruses.
Language	English
Publishing date	2021-03-30
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	205723-2
ISSN	1090-2104 ; 0006-291X ; 0006-291X
ISSN (online)	1090-2104 ; 0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2021.03.099
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Article ; Online: Hsp104-dependent ability to assimilate mannitol and sorbitol conferred by a truncated Cyc8 with a C-terminal polyglutamine in Saccharomyces cerevisiae.

Tanaka, Hideki / Murata, Kousaku / Hashimoto, Wataru / Kawai, Shigeyuki

PloS one

2020 Volume 15, Issue 11, Page(s) e0242054

Abstract: Tup1-Cyc8 (also known as Tup1-Ssn6) is a general transcriptional corepressor. D-Mannitol (mannitol) and D-sorbitol (sorbitol) are the major polyols in nature. Budding yeast Saccharomyces cerevisiae is unable to assimilate mannitol or sorbitol, but ... ...

Abstract	Tup1-Cyc8 (also known as Tup1-Ssn6) is a general transcriptional corepressor. D-Mannitol (mannitol) and D-sorbitol (sorbitol) are the major polyols in nature. Budding yeast Saccharomyces cerevisiae is unable to assimilate mannitol or sorbitol, but acquires the ability to assimilate mannitol due to a spontaneous mutation in TUP1 or CYC8. In this study, we found that spontaneous mutation of TUP1 or CYC8 also permitted assimilation of sorbitol. Some spontaneous nonsense mutations of CYC8 produced a truncated Cyc8 with a C-terminal polyglutamine. The effects were guanidine hydrochloride-sensitive and were dependent on Hsp104, but were complemented by introduction of CYC8, ruling out involvement of a prion. Assimilation of mannitol and sorbitol conferred by other mutations of TUP1 or CYC8 was guanidine hydrochloride-tolerant. It is physiologically reasonable that S. cerevisiae carries this mechanism to acquire the ability to assimilate major polyols in nature.
MeSH term(s)	Codon, Nonsense ; Gene Expression Regulation, Fungal ; Heat-Shock Proteins/metabolism ; Mannitol/metabolism ; Nuclear Proteins/genetics ; Peptides/metabolism ; Promoter Regions, Genetic ; Protein Domains ; Repressor Proteins/chemistry ; Repressor Proteins/genetics ; Repressor Proteins/metabolism ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/growth & development ; Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae Proteins/chemistry ; Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism ; Sorbitol/metabolism
Chemical Substances	CYC8 protein, S cerevisiae ; Codon, Nonsense ; Heat-Shock Proteins ; Nuclear Proteins ; Peptides ; Repressor Proteins ; Saccharomyces cerevisiae Proteins ; TUP1 protein, S cerevisiae ; HsP104 protein, S cerevisiae (143012-44-6) ; polyglutamine (26700-71-0) ; Mannitol (3OWL53L36A) ; Sorbitol (506T60A25R)
Language	English
Publishing date	2020-11-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0242054
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Article ; Online: Hsp104-dependent ability to assimilate mannitol and sorbitol conferred by a truncated Cyc8 with a C-terminal polyglutamine in Saccharomyces cerevisiae.

Hideki Tanaka / Kousaku Murata / Wataru Hashimoto / Shigeyuki Kawai

PLoS ONE, Vol 15, Iss 11, p e

2020 Volume 0242054

Abstract	Tup1-Cyc8 (also known as Tup1-Ssn6) is a general transcriptional corepressor. D-Mannitol (mannitol) and D-sorbitol (sorbitol) are the major polyols in nature. Budding yeast Saccharomyces cerevisiae is unable to assimilate mannitol or sorbitol, but acquires the ability to assimilate mannitol due to a spontaneous mutation in TUP1 or CYC8. In this study, we found that spontaneous mutation of TUP1 or CYC8 also permitted assimilation of sorbitol. Some spontaneous nonsense mutations of CYC8 produced a truncated Cyc8 with a C-terminal polyglutamine. The effects were guanidine hydrochloride-sensitive and were dependent on Hsp104, but were complemented by introduction of CYC8, ruling out involvement of a prion. Assimilation of mannitol and sorbitol conferred by other mutations of TUP1 or CYC8 was guanidine hydrochloride-tolerant. It is physiologically reasonable that S. cerevisiae carries this mechanism to acquire the ability to assimilate major polyols in nature.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
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Article ; Online: Synergistic effect of non-neutralizing antibodies and interferon-γ for cross-protection against influenza.

Shibuya, Meito / Tamiya, Shigeyuki / Kawai, Atsushi / Hirai, Toshiro / Cragg, Mark S / Yoshioka, Yasuo

iScience

2021 Volume 24, Issue 10, Page(s) 103131

Abstract: Current influenza vaccines do not typically confer cross-protection against antigenically mismatched strains. To develop vaccines conferring broader cross-protection, recent evidence indicates the crucial role of both cross-reactive antibodies and viral- ... ...

Abstract	Current influenza vaccines do not typically confer cross-protection against antigenically mismatched strains. To develop vaccines conferring broader cross-protection, recent evidence indicates the crucial role of both cross-reactive antibodies and viral-specific CD4
Language	English
Publishing date	2021-09-15
Publishing country	United States
Document type	Journal Article
ISSN	2589-0042
ISSN (online)	2589-0042
DOI	10.1016/j.isci.2021.103131
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Uncovering the reactive nature of 4-deoxy-L-erythro-5-hexoseulose uronate for the utilization of alginate, a promising marine biopolymer.

Nakata, Shota / Murata, Kousaku / Hashimoto, Wataru / Kawai, Shigeyuki

Scientific reports

2019 Volume 9, Issue 1, Page(s) 17147

Abstract: Alginate is a linear polyuronate in brown macroalgae. It is also a promising marine biopolymer that can be degraded by exo-type alginate lyase into an unsaturated uronate that is non-enzymatically or enzymatically converted to 4-deoxy-L-erythro-5- ... ...

Abstract	Alginate is a linear polyuronate in brown macroalgae. It is also a promising marine biopolymer that can be degraded by exo-type alginate lyase into an unsaturated uronate that is non-enzymatically or enzymatically converted to 4-deoxy-L-erythro-5-hexoseulose uronate (DEH). In a bioengineered yeast Saccharomyces cerevisiae (DEH++) strain that utilizes DEH, DEH is not only an important physiological metabolite but also a promising carbon source for biorefinery systems. In this study, we uncovered the essential chemical nature of DEH. In particular, we showed that DEH non-enzymatically reacts with specific amino groups in Tris, ammonium salts [(NH
MeSH term(s)	Alginates/metabolism ; Amino Acids/metabolism ; Ammonium Compounds/metabolism ; Biopolymers/metabolism ; Furans/metabolism ; Nitrates/metabolism ; Nitrogen/metabolism ; Saccharomyces cerevisiae/metabolism ; Uronic Acids/metabolism
Chemical Substances	Alginates ; Amino Acids ; Ammonium Compounds ; Biopolymers ; Furans ; Nitrates ; Uronic Acids ; sodium nitrate (8M4L3H2ZVZ) ; Nitrogen (N762921K75) ; 2-furoic acid (P577F6494A)
Language	English
Publishing date	2019-11-20
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-019-53597-1
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Article ; Online: Biofuel Production Based on Carbohydrates from Both Brown and Red Macroalgae: Recent Developments in Key Biotechnologies.

Kawai, Shigeyuki / Murata, Kousaku

International journal of molecular sciences

2016 Volume 17, Issue 2, Page(s) 145

Abstract: Marine macroalgae (green, red and brown macroalgae) have attracted attention as an alternative source of renewable biomass for producing both fuels and chemicals due to their high content of suitable carbohydrates and to their advantages over terrestrial ...

Abstract	Marine macroalgae (green, red and brown macroalgae) have attracted attention as an alternative source of renewable biomass for producing both fuels and chemicals due to their high content of suitable carbohydrates and to their advantages over terrestrial biomass. However, except for green macroalgae, which contain relatively easily-fermentable glucans as their major carbohydrates, practical utilization of red and brown macroalgae has been regarded as difficult due to the major carbohydrates (alginate and mannitol of brown macroalgae and 3,6-anhydro-L-galactose of red macroalgae) not being easily fermentable. Recently, several key biotechnologies using microbes have been developed enabling utilization of these brown and red macroalgal carbohydrates as carbon sources for the production of fuels (ethanol). In this review, we focus on these recent developments with emphasis on microbiological biotechnologies.
MeSH term(s)	Biofuels ; Biotechnology ; Carbohydrate Metabolism ; Ethanol/metabolism ; Fermentation ; Seaweed/metabolism
Chemical Substances	Biofuels ; Ethanol (3K9958V90M)
Language	English
Publishing date	2016-02-06
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms17020145
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Article ; Online: Synergistic effect of non-neutralizing antibodies and interferon-γ for cross-protection against influenza

Meito Shibuya / Shigeyuki Tamiya / Atsushi Kawai / Toshiro Hirai / Mark S. Cragg / Yasuo Yoshioka

iScience, Vol 24, Iss 10, Pp 103131- (2021)

2021

Abstract: Summary: Current influenza vaccines do not typically confer cross-protection against antigenically mismatched strains. To develop vaccines conferring broader cross-protection, recent evidence indicates the crucial role of both cross-reactive antibodies ... ...

Abstract	Summary: Current influenza vaccines do not typically confer cross-protection against antigenically mismatched strains. To develop vaccines conferring broader cross-protection, recent evidence indicates the crucial role of both cross-reactive antibodies and viral-specific CD4+ T cells; however, the precise mechanism of cross-protection is unclear. Furthermore, adjuvants that can efficiently induce cross-protective CD4+ T cells have not been identified. Here we show that CpG oligodeoxynucleotides combined with aluminum salts work as adjuvants for influenza vaccine and confer strong cross-protection in mice. Both cross-reactive antibodies and viral-specific CD4+ T cells contributed to cross-protection synergistically, with each individually ineffective. Furthermore, we found that downregulated expression of Fcγ receptor IIb on alveolar macrophages due to IFN-γ secreted by viral-specific CD4+ T cells improves the activity of cross-reactive antibodies. Our findings inform the development of optimal adjuvants for vaccines and how influenza vaccines confer broader cross-protection.
Keywords	Biological sciences ; Immunology ; Immune response ; Science ; Q
Subject code	570
Language	English
Publishing date	2021-10-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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