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Article ; Online: Where and How Extensive Are the Living Areas of the Elderly? An Empirical Study on 'Ageing in Place' in Three Small and Medium-Sized Japanese Cities.

Nishino, Tatsuya

International journal of environmental research and public health

2021 Volume 18, Issue 11

Abstract: The policies regarding the elderly in advanced countries are based on the notion of 'ageing in place'. The question arises, where and how extensive can the 'place' be? Is there a method of estimating a senior's living area? The purpose of this study was ... ...

Abstract	The policies regarding the elderly in advanced countries are based on the notion of 'ageing in place'. The question arises, where and how extensive can the 'place' be? Is there a method of estimating a senior's living area? The purpose of this study was to determine the common characteristics of the living areas of seniors in three small and medium-sized Japanese cities. The basic methodology involved a comparative analysis involving these cities. We used case studies to cross tabulate interviews regarding the daily outings of participants, some of whom needed long-term care while others did not. The data covered a total of 727 participants, 307 of whom needed long-term care and 420 requiring none. Comparative analysis revealed the common characteristics of living areas for seniors in these cities, i.e., two-layered living areas of healthy seniors; fewer outings on foot due to frailty; the average moving time via transportation is approximately 12 min; and living areas overlap districts where hospitals and stores are located. The results indicate that we can roughly estimate the living areas of seniors in any neighborhood to investigate accessibility to nearby hospitals and stores.
MeSH term(s)	Aged ; Aging ; Cities ; Humans ; Independent Living ; Japan ; Residence Characteristics
Language	English
Publishing date	2021-06-02
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1660-4601
ISSN (online)	1660-4601
DOI	10.3390/ijerph18115960
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Article ; Online: Where and How Extensive Are the Living Areas of the Elderly? An Empirical Study on ‘Ageing in Place’ in Three Small and Medium-Sized Japanese Cities

Tatsuya Nishino

International Journal of Environmental Research and Public Health, Vol 18, Iss 5960, p

2021 Volume 5960

Abstract: The policies regarding the elderly in advanced countries are based on the notion of ‘ageing in place’. The question arises, where and how extensive can the ‘place’ be? Is there a method of estimating a senior’s living area? The purpose of this study was ... ...

Abstract	The policies regarding the elderly in advanced countries are based on the notion of ‘ageing in place’. The question arises, where and how extensive can the ‘place’ be? Is there a method of estimating a senior’s living area? The purpose of this study was to determine the common characteristics of the living areas of seniors in three small and medium-sized Japanese cities. The basic methodology involved a comparative analysis involving these cities. We used case studies to cross tabulate interviews regarding the daily outings of participants, some of whom needed long-term care while others did not. The data covered a total of 727 participants, 307 of whom needed long-term care and 420 requiring none. Comparative analysis revealed the common characteristics of living areas for seniors in these cities, i.e., two-layered living areas of healthy seniors; fewer outings on foot due to frailty; the average moving time via transportation is approximately 12 min; and living areas overlap districts where hospitals and stores are located. The results indicate that we can roughly estimate the living areas of seniors in any neighborhood to investigate accessibility to nearby hospitals and stores.
Keywords	ageing in place ; the elderly ; seniors ; living area ; small and medium-sized city ; Japan ; Medicine ; R
Subject code	720
Language	English
Publishing date	2021-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Biochemical and crystallization analysis of the CENP-SX-DNA complex.

Ito, Sho / Nishino, Tatsuya

Acta crystallographica. Section F, Structural biology communications

2022 Volume 78, Issue Pt 5, Page(s) 193–199

Abstract: The CENP-SX (MHF) complex is a conserved histone-fold protein complex that is involved in chromosome segregation and DNA repair. It can bind to DNA on its own as well as in complex with other proteins such as CENP-TW and FANCM to recognize specific ... ...

Abstract	The CENP-SX (MHF) complex is a conserved histone-fold protein complex that is involved in chromosome segregation and DNA repair. It can bind to DNA on its own as well as in complex with other proteins such as CENP-TW and FANCM to recognize specific substrates. CENP-SX binds nonspecifically to dsDNA, similar to other histone-fold proteins. Several low-resolution structures of CENP-SX in complex with DNA are known, but a high-resolution structure is still lacking. The DNA-binding properties of CENP-SX and FANCM-CENP-SX complexes with various lengths of dsDNA were compared and the band-shift patterns and migration positions were found to differ. To confirm the DNA-binding properties in detail, CENP-SX-DNA and FANCM-CENP-SX-DNA complexes were crystallized. Analysis of the crystals revealed that they all contained the CENP-SX-DNA complex, irrespective of the complex that was used in crystallization. Detailed diffraction data analyses revealed that there were two types of crystal with different space groups, P2
MeSH term(s)	Crystallization ; Crystallography, X-Ray ; DNA/chemistry ; DNA-Binding Proteins/chemistry ; Histones
Chemical Substances	DNA-Binding Proteins ; Histones ; DNA (9007-49-2)
Language	English
Publishing date	2022-04-22
Publishing country	United States
Document type	Journal Article
ISSN	2053-230X
ISSN (online)	2053-230X
DOI	10.1107/S2053230X22003843
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Article ; Online: Quantitative Properties of the Macro Supply and Demand Structure for Care Facilities for Elderly in Japan.

Nishino, Tatsuya

International journal of environmental research and public health

2017 Volume 14, Issue 12

Abstract: As the Asian country with the most aged population, Japan, has been modifying its social welfare system. In 2000, the Japanese social care vision turned towards meeting the elderly's care needs in their own homes with proper formal care services. This ... ...

Abstract	As the Asian country with the most aged population, Japan, has been modifying its social welfare system. In 2000, the Japanese social care vision turned towards meeting the elderly's care needs in their own homes with proper formal care services. This study aims to understand the quantitative properties of the macro supply and demand structure for facilities for the elderly who require support or long-term care throughout Japan and present them as index values. Additionally, this study compares the targets for establishing long-term care facilities set by Japan's Ministry of Health, Labor and Welfare for 2025. In 2014, approximately 90% of all the people who were certified as requiring support and long-term care and those receiving preventive long-term care or long-term care services, were 75 years or older. The target increases in the number of established facilities by 2025 (for the 75-years-or-older population) were calculated to be 3.3% for nursing homes; 2.71% for long-term-care health facilities; 1.7% for group living facilities; and, 1.84% for community-based multi-care facilities. It was revealed that the establishment targets for 2025 also increase over current projections with the expected increase of the absolute number of users of group living facilities and community-based multi-care facilities. On the other hand, the establishment target for nursing homes remains almost the same as the current projection, whereas that for long-term-care health facilities decreases. These changes of facility ratios reveal that the Japanese social care system is shifting to realize 'Ageing in Place'. When considering households' tendencies, the target ratios for established facilities are expected to be applied to the other countries in Asia.
MeSH term(s)	Aged ; Female ; Health Services Needs and Demand ; Humans ; Insurance, Long-Term Care ; Japan ; Long-Term Care/trends ; Male ; Residential Facilities/supply & distribution
Language	English
Publishing date	2017-12-01
Publishing country	Switzerland
Document type	Journal Article
ISSN	1660-4601
ISSN (online)	1660-4601
DOI	10.3390/ijerph14121489
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Article ; Online: Structural analysis of the chicken FANCM-MHF complex and its stability.

Ito, Sho / Nishino, Tatsuya

Acta crystallographica. Section F, Structural biology communications

2021 Volume 77, Issue Pt 1, Page(s) 1–7

Abstract: FANCM is involved in eukaryotic DNA-damage recognition and activates the Fanconi anemia (FA) pathway through complex formation. MHF is one of the FANCM-associating components and contains a histone-fold DNA-binding domain. Loss of the FANCM-MHF ... ...

Abstract	FANCM is involved in eukaryotic DNA-damage recognition and activates the Fanconi anemia (FA) pathway through complex formation. MHF is one of the FANCM-associating components and contains a histone-fold DNA-binding domain. Loss of the FANCM-MHF interaction compromises the activation of the FA pathway, resulting in chromosomal instability. Thus, formation of the FANCM-MHF complex is important for function, but its nature largely remains elusive. Here, the aim was to reveal the molecular and structural basis for the stability of the FANCM-MHF complex. A recombinant tripartite complex containing chicken FANCM (MHF interaction region), MHF1 and MHF2 was expressed and purified. The purified tripartite complex was crystallized under various conditions and three different crystals were obtained from similar crystallization conditions. Unexpectedly, structure determination revealed that one of the crystals contained the FANCM-MHF complex but that the other two contained the MHF complex without FANCM. How FANCM dissociates from MHF was further investigated and it was found that the presence of 2-methyl-2,4-pentanediol (MPD) and an oxidative environment may have promoted its release. However, under these conditions MHF retained its complexed form. FANCM-MHF interaction involves a mixture of hydrophobic/hydrophilic interactions, and chicken FANCM contains several nonconserved cysteines within this region which may lead to aggregation with other FANCM-MHF molecules. These results indicate an unexpected nature of the FANCM-MHF complex and the data can be used to improve the stability of the complex for biochemical and structural analyses.
MeSH term(s)	Animals ; Apoptosis Regulatory Proteins/chemistry ; Apoptosis Regulatory Proteins/genetics ; Apoptosis Regulatory Proteins/metabolism ; Chickens ; Crystallography, X-Ray ; DNA Helicases/chemistry ; DNA Helicases/genetics ; DNA Helicases/metabolism ; Glycols/chemistry ; Models, Molecular ; Multiprotein Complexes/chemistry ; Multiprotein Complexes/genetics ; Multiprotein Complexes/metabolism ; Protein Conformation ; Protein Stability
Chemical Substances	Apoptosis Regulatory Proteins ; Glycols ; Multiprotein Complexes ; DNA Helicases (EC 3.6.4.-) ; hexylene glycol (KEH0A3F75J)
Language	English
Publishing date	2021-01-01
Publishing country	United States
Document type	Journal Article
ISSN	2053-230X
ISSN (online)	2053-230X
DOI	10.1107/S2053230X20016003
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Quantitative Properties of the Macro Supply and Demand Structure for Care Facilities for Elderly in Japan

Tatsuya Nishino

International Journal of Environmental Research and Public Health, Vol 14, Iss 12, p

2017 Volume 1489

Abstract: As the Asian country with the most aged population, Japan, has been modifying its social welfare system. In 2000, the Japanese social care vision turned towards meeting the elderly’s care needs in their own homes with proper formal care services. This ... ...

Abstract	As the Asian country with the most aged population, Japan, has been modifying its social welfare system. In 2000, the Japanese social care vision turned towards meeting the elderly’s care needs in their own homes with proper formal care services. This study aims to understand the quantitative properties of the macro supply and demand structure for facilities for the elderly who require support or long-term care throughout Japan and present them as index values. Additionally, this study compares the targets for establishing long-term care facilities set by Japan’s Ministry of Health, Labor and Welfare for 2025. In 2014, approximately 90% of all the people who were certified as requiring support and long-term care and those receiving preventive long-term care or long-term care services, were 75 years or older. The target increases in the number of established facilities by 2025 (for the 75-years-or-older population) were calculated to be 3.3% for nursing homes; 2.71% for long-term-care health facilities; 1.7% for group living facilities; and, 1.84% for community-based multi-care facilities. It was revealed that the establishment targets for 2025 also increase over current projections with the expected increase of the absolute number of users of group living facilities and community-based multi-care facilities. On the other hand, the establishment target for nursing homes remains almost the same as the current projection, whereas that for long-term-care health facilities decreases. These changes of facility ratios reveal that the Japanese social care system is shifting to realize ‘Ageing in Place’. When considering households’ tendencies, the target ratios for established facilities are expected to be applied to the other countries in Asia.
Keywords	the elderly ; quantitative index ; group living facilities ; community-based multi-care facility ; the Long-Term Care Insurance Act ; Medicine ; R
Subject code	360
Language	English
Publishing date	2017-12-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Pulmonary Erdheim-Chester Disease With BRAF-AGAP3 Fusion: Late-Onset Osteolytic Femoral Lesions Despite Long-Term Pulmonary Stabilization With Corticosteroid.

Nishino, Koichi / Takagi, Tatsuya / Hayashi, Takuo / Kunimine, Shinya / Tsuchihashi, Hitoshi / Kato, Shunsuke / Takahashi, Kazuhisa / Seyama, Kuniaki

Cureus

2024 Volume 16, Issue 3, Page(s) e55670

Abstract: Erdheim-Chester disease (ECD) is a rare inflammatory myeloid neoplasm affecting multiple systems and organs. The patient is a 38-year-old male with ECD complicated with pulmonary and cutaneous manifestations but without bone lesions diagnosed in 2008. ... ...

Abstract	Erdheim-Chester disease (ECD) is a rare inflammatory myeloid neoplasm affecting multiple systems and organs. The patient is a 38-year-old male with ECD complicated with pulmonary and cutaneous manifestations but without bone lesions diagnosed in 2008. Initial treatment with oral and inhaled corticosteroids achieved persistent favorable disease remission. However, atypical late-onset bone lesions developed in the bilateral femur in 2021. Although
Language	English
Publishing date	2024-03-06
Publishing country	United States
Document type	Case Reports
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.55670
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Article ; Online: Ubiquitin-Derived Fragment as a Peptide Linker for the Efficient Cleavage of a Target Protein from a Degron.

Utsugi, Yuki / Nishimura, Ken / Yamanaka, Satoshi / Nishino, Kohei / Kosako, Hidetaka / Sawasaki, Tatsuya / Shigemori, Hideyuki / Wandless, Thomas J / Miyamae, Yusaku

ACS chemical biology

2024 Volume 19, Issue 2, Page(s) 497–505

Abstract: The chemogenetic control of cellular protein stability using degron tags is a powerful experimental strategy in biomedical research. However, this technique requires permanent fusion of the degron to a target protein, which may interfere with the proper ... ...

Abstract	The chemogenetic control of cellular protein stability using degron tags is a powerful experimental strategy in biomedical research. However, this technique requires permanent fusion of the degron to a target protein, which may interfere with the proper function of the protein. Here, we report a peptide fragment from the carboxyl terminus of ubiquitin as a cleavable linker that exhibits the slow but efficient cleavage of a degron tag via cellular deubiquitinating enzymes (DUBs). We designed a fusion protein consisting of a cleavable linker and a destabilizing domain (DD), which conditionally controls the expression and release of a target protein in a ligand-induced state, allowing the free unmodified protein to perform its function. Insertion of an AGIA epitope at the carboxyl terminus of the linker made space for the DUBs to access the site to assist the cleavage reaction when the amino terminus of the target protein caused steric hindrance. The developed system, termed a cleavable degron using ubiquitin-derived linkers (c-DUB), provides robust and tunable regulation of target proteins in their native forms. The c-DUB system is a useful tool for the regulation of proteins that have terminal sites that are essential for the proper localization and function. In addition, a mechanistic investigation using proximity labeling showed that DUBs associate with the refolded DD to reverse ubiquitination, suggesting a cellular surveillance system for distinguishing the refolded DD from misfolded proteins. The c-DUB method may benefit from this machinery so that DUBs subsequently cleave the neighboring linker.
MeSH term(s)	Ubiquitin/metabolism ; Degrons ; Proteins/metabolism ; Ubiquitination ; Peptides/metabolism
Chemical Substances	Ubiquitin ; Proteins ; Peptides
Language	English
Publishing date	2024-01-25
Publishing country	United States
Document type	Journal Article
ISSN	1554-8937
ISSN (online)	1554-8937
DOI	10.1021/acschembio.3c00670
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Article ; Online: Dropped Head Syndrome Caused by Immune-mediated Necrotizing Myopathy.

Ueno, Tatsuya / Suzuki, Chieko / Nishino, Ichizo / Tomiyama, Masahiko

Internal medicine (Tokyo, Japan)

2019 Volume 58, Issue 22, Page(s) 3343–3344

Language	English
Publishing date	2019-07-22
Publishing country	Japan
Document type	Journal Article
ZDB-ID	32371-8
ISSN	1349-7235 ; 0021-5120 ; 0918-2918
ISSN (online)	1349-7235
ISSN	0021-5120 ; 0918-2918
DOI	10.2169/internalmedicine.2930-19
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Article ; Online: Establishment of a novel 70K Mac-2 binding protein antibody through screening of fucosylation-related antibodies.

Masuda, Mika / Asuka, Tatsuya / Terao, Naoko / Nishino, Shinsuke / Ikeda, Shun / Takamatsu, Shinji / Kondo, Jumpei / Miyoshi, Eiji

Journal of biochemistry

2023 Volume 173, Issue 6, Page(s) 487–495

Abstract: Mac-2 binding protein (Mac-2bp) is a serum glycoprotein that contains seven N-glycans, and Mac-2bp serum levels are increased in patients with several types of cancer and liver disease. Mac-2bp glycosylation isomer has been applied as a clinical ... ...

Abstract	Mac-2 binding protein (Mac-2bp) is a serum glycoprotein that contains seven N-glycans, and Mac-2bp serum levels are increased in patients with several types of cancer and liver disease. Mac-2bp glycosylation isomer has been applied as a clinical biomarker of several diseases, including liver fibrosis. In the present study, we identified fucosylated Mac-2bp in the conditioned medium of cancer cells resistant to anticancer therapies using glycoproteomic analyses. Fucosylation is one of the most important types of glycosylation involved in carcinogenesis and cancer stemness. To establish a next-generation glycan antibody for fucosylated Mac-2bp, we used fucosylation-deficient HEK293T cells to prepare reference Mac-2bp antigens and performed antibody screening. Unexpectedly, the 19-8H mAb obtained with our screen recognized 70K Mac-2bp, which is C-terminus-truncated product, rather than specifically recognizing fucosylated Mac-2bp. We performed immunocytochemistry using our novel 19-8H mAb, which resulted in strong cell surface staining of anticancer drug-resistant cancer cells. Therefore, our novel 19-8H mAb represents a valuable tool for cancer biology research that can help elucidate the biological function of 70K Mac-2bp.
MeSH term(s)	Humans ; Antibodies/metabolism ; Glycoproteins ; Glycosylation ; HEK293 Cells ; Membrane Glycoproteins/metabolism
Chemical Substances	Antibodies ; Glycoproteins ; Membrane Glycoproteins ; TAA90K protein, human
Language	English
Publishing date	2023-02-10
Publishing country	England
Document type	Journal Article
ZDB-ID	218073-x
ISSN	1756-2651 ; 0021-924X
ISSN (online)	1756-2651
ISSN	0021-924X
DOI	10.1093/jb/mvad015
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