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Article: An Overview of D7 Protein Structure and Physiological Roles in Blood-Feeding Nematocera.

Alvarenga, Patricia H / Andersen, John F

2022 Volume 12, Issue 1

Abstract: Each time an insect bites a vertebrate host, skin and vascular injury caused by piercing triggers a series of responses including hemostasis, inflammation and immunity. In place, this set of redundant and interconnected responses would ultimately cause ... ...

Abstract	Each time an insect bites a vertebrate host, skin and vascular injury caused by piercing triggers a series of responses including hemostasis, inflammation and immunity. In place, this set of redundant and interconnected responses would ultimately cause blood coagulation, itching and pain leading to host awareness, resulting in feeding interruption in the best-case scenario. Nevertheless, hematophagous arthropod saliva contains a complex cocktail of molecules that are crucial to the success of blood-feeding. Among important protein families described so far in the saliva of blood sucking arthropods, is the D7, abundantly expressed in blood feeding Nematocera. D7 proteins are distantly related to insect Odorant-Binding Proteins (OBP), and despite low sequence identity, observation of structural similarity led to the suggestion that like OBPs, they should bind/sequester small hydrophobic compounds. Members belonging to this family are divided in short forms and long forms, containing one or two OBP-like domains, respectively. Here, we provide a review of D7 proteins structure and function, discussing how gene duplication and some modifications in their OBP-like domains during the course of evolution lead to gain and loss of function among different hematophagous Diptera species.
Language	English
Publishing date	2022-12-26
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2661517-4
ISSN	2079-7737
ISSN	2079-7737
DOI	10.3390/biology12010039
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: A Deeper Insight into the Tick Salivary Protein Families under the Light of Alphafold2 and Dali: Introducing the TickSialoFam 2.0 Database.

Mans, Ben J / Andersen, John F / Ribeiro, José M C

International journal of molecular sciences

2022 Volume 23, Issue 24

Abstract: Hard ticks feed for several days or weeks on their hosts and their saliva contains thousands of polypeptides belonging to dozens of families, as identified by salivary transcriptomes. Comparison of the coding sequences to protein databases helps to ... ...

Abstract	Hard ticks feed for several days or weeks on their hosts and their saliva contains thousands of polypeptides belonging to dozens of families, as identified by salivary transcriptomes. Comparison of the coding sequences to protein databases helps to identify putative secreted proteins and their potential functions, directing and focusing future studies, usually done with recombinant proteins that are tested in different bioassays. However, many families of putative secreted peptides have a unique character, not providing significant matches to known sequences. The availability of the Alphafold2 program, which provides in silico predictions of the 3D polypeptide structure, coupled with the Dali program which uses the atomic coordinates of a structural model to search the Protein Data Bank (PDB) allows another layer of investigation to annotate and ascribe a functional role to proteins having so far being characterized as "unique". In this study, we analyzed the classification of tick salivary proteins under the light of the Alphafold2/Dali programs, detecting novel protein families and gaining new insights relating the structure and function of tick salivary proteins.
Language	English
Publishing date	2022-12-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms232415613
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Acid phosphatase-like proteins, a biogenic amine and leukotriene-binding salivary protein family from the flea Xenopsylla cheopis.

Lu, Stephen / Andersen, John F / Bosio, Christopher F / Hinnebusch, B Joseph / Ribeiro, José M

Communications biology

2023 Volume 6, Issue 1, Page(s) 1280

Abstract: The salivary glands of hematophagous arthropods contain pharmacologically active molecules that interfere with host hemostasis and immune responses, favoring blood acquisition and pathogen transmission. Exploration of the salivary gland composition of ... ...

Abstract	The salivary glands of hematophagous arthropods contain pharmacologically active molecules that interfere with host hemostasis and immune responses, favoring blood acquisition and pathogen transmission. Exploration of the salivary gland composition of the rat flea, Xenopsylla cheopis, revealed several abundant acid phosphatase-like proteins whose sequences lacked one or two of their presumed catalytic residues. In this study, we undertook a comprehensive characterization of the tree most abundant X. cheopis salivary acid phosphatase-like proteins. Our findings indicate that the three recombinant proteins lacked the anticipated catalytic activity and instead, displayed the ability to bind different biogenic amines and leukotrienes with high affinity. Moreover, X-ray crystallography data from the XcAP-1 complexed with serotonin revealed insights into their binding mechanisms.
MeSH term(s)	Rats ; Animals ; Siphonaptera/physiology ; Xenopsylla ; Acid Phosphatase ; Salivary Proteins and Peptides/genetics ; Biogenic Amines ; Leukotrienes
Chemical Substances	Acid Phosphatase (EC 3.1.3.2) ; Salivary Proteins and Peptides ; Biogenic Amines ; Leukotrienes
Language	English
Publishing date	2023-12-18
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2399-3642
ISSN (online)	2399-3642
DOI	10.1038/s42003-023-05679-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Assessing anatomical distribution of atopic dermatitis identifies a cluster of patients with late onset and low risk of asthma and allergy: An observational study.

Ali, Zarqa / Anderson, Kathryn / Andersen, Anders D / Dahiya, Priyanka / Zibert, John R / Thomsen, Simon F

Health science reports

2023 Volume 6, Issue 5, Page(s) e1219

Abstract: Background and aims: A better understanding of distinct subgroups in atopic dermatitis (AD) is warranted. The aim was to identify and determine characteristics of clusters based on anatomical location of AD.: Methods: In this 8-week, observational, ... ...

Abstract	Background and aims: A better understanding of distinct subgroups in atopic dermatitis (AD) is warranted. The aim was to identify and determine characteristics of clusters based on anatomical location of AD. Methods: In this 8-week, observational, decentralized study, patients with AD completed a baseline questionnaire about anatomical location and severity of AD, and a principal component analysis (PCA) was applied to identify clusters. The Patient-Oriented Eczema Measure (POEM) was completed weekly and photographs of affected body areas were captured by the participants' own smartphones. From the weekly photographs, the AD severity was evaluated using the intensity part of the SCORing Atopic Dermatitis. Results: Fifty-five participants were recruited, of which 53 completed the baseline questionnaire with a mean POEM of 14.5 (SD: 5.6). The PCA analysis revealed three clusters, with AD predominantly on the shins, knees, and genitals (Cluster 1), with involvement of the upper body (Cluster 2), and with AD on the hands and feet (Cluster 3). Cluster 1 had a lower mean POEM score (11.12, SD: 5.3) compared with Clusters 2 (12.64, SD: 4.5) and 3 (15.98, SD: 4.7), respectively ( Conclusion: Three clusters of patients with AD based on affected body areas were identified. The cluster with involvement of legs and genitals was characterized by the oldest age of AD onset and the lowest prevalence of asthma/allergy.
Language	English
Publishing date	2023-05-04
Publishing country	United States
Document type	Journal Article
ISSN	2398-8835
ISSN (online)	2398-8835
DOI	10.1002/hsr2.1219
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Article: Publisher's Note: Shuttle-box systems for studying preferred environmental ranges by aquatic animals.

Christensen, Emil A F / Andersen, Lars E J / Bergsson, Heiðrikur / Steffensen, John F / Killen, Shaun S

Conservation physiology

2021 Volume 9, Issue 1, Page(s) coab051

Language	English
Publishing date	2021-06-29
Publishing country	England
Document type	Journal Article
ZDB-ID	2721508-8
ISSN	2051-1434
ISSN	2051-1434
DOI	10.1093/conphys/coab051
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: An Overview of D7 Protein Structure and Physiological Roles in Blood-Feeding Nematocera

Alvarenga, Patricia H. / Andersen, John F.

Biology (Basel). 2022 Dec. 26, v. 12, no. 1

2022

Abstract	Each time an insect bites a vertebrate host, skin and vascular injury caused by piercing triggers a series of responses including hemostasis, inflammation and immunity. In place, this set of redundant and interconnected responses would ultimately cause blood coagulation, itching and pain leading to host awareness, resulting in feeding interruption in the best-case scenario. Nevertheless, hematophagous arthropod saliva contains a complex cocktail of molecules that are crucial to the success of blood-feeding. Among important protein families described so far in the saliva of blood sucking arthropods, is the D7, abundantly expressed in blood feeding Nematocera. D7 proteins are distantly related to insect Odorant-Binding Proteins (OBP), and despite low sequence identity, observation of structural similarity led to the suggestion that like OBPs, they should bind/sequester small hydrophobic compounds. Members belonging to this family are divided in short forms and long forms, containing one or two OBP-like domains, respectively. Here, we provide a review of D7 proteins structure and function, discussing how gene duplication and some modifications in their OBP-like domains during the course of evolution lead to gain and loss of function among different hematophagous Diptera species.
Keywords	Diptera ; blood ; blood coagulation ; evolution ; gene duplication ; hematophagous arthropods ; hematophagy ; hemostasis ; hydrophobicity ; immunity ; inflammation ; insects ; pain ; protein structure ; saliva ; sequence analysis ; vertebrates
Language	English
Dates of publication	2022-1226
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2661517-4
ISSN	2079-7737
ISSN	2079-7737
DOI	10.3390/biology12010039
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Integrated analysis of the sialotranscriptome and sialoproteome of the rat flea Xenopsylla cheopis.

Lu, Stephen / Andersen, John F / Bosio, Christopher F / Hinnebusch, B Joseph / Ribeiro, José M C

Journal of proteomics

2022 Volume 254, Page(s) 104476

Abstract: Over the last 20 years, advances in sequencing technologies paired with biochemical and structural studies have shed light on the unique pharmacological arsenal produced by the salivary glands of hematophagous arthropods that can target host hemostasis ... ...

Abstract	Over the last 20 years, advances in sequencing technologies paired with biochemical and structural studies have shed light on the unique pharmacological arsenal produced by the salivary glands of hematophagous arthropods that can target host hemostasis and immune response, favoring blood acquisition and, in several cases, enhancing pathogen transmission. Here we provide a deeper insight into Xenopsylla cheopis salivary gland contents pairing transcriptomic and proteomic approaches. Sequencing of 99 pairs of salivary glands from adult female X. cheopis yielded a total of 7432 coding sequences functionally classified into 25 classes, of which the secreted protein class was the largest. The translated transcripts also served as a reference database for the proteomic study, which identified peptides from 610 different proteins. Both approaches revealed that the acid phosphatase family is the most abundant salivary protein group from X. cheopis. Additionally, we report here novel sequences similar to the FS-H family, apyrases, odorant and hormone-binding proteins, antigen 5-like proteins, adenosine deaminases, peptidase inhibitors from different subfamilies, proteins rich in Glu, Gly, and Pro residues, and several potential secreted proteins with unknown function. SIGNIFICANCE: The rat flea X. cheopis is the main vector of Yersinia pestis, the etiological agent of the bubonic plague responsible for three major pandemics that marked human history and remains a burden to human health. In addition to Y. pestis fleas can also transmit other medically relevant pathogens including Rickettsia spp. and Bartonella spp. The studies of salivary proteins from other hematophagous vectors highlighted the importance of such molecules for blood acquisition and pathogen transmission. However, despite the historical and clinical importance of X. cheopis little is known regarding their salivary gland contents and potential activities. Here we provide a comprehensive analysis of X. cheopis salivary composition using next generation sequencing methods paired with LC-MS/MS analysis, revealing its unique composition compared to the sialomes of other blood-feeding arthropods, and highlighting the different pathways taken during the evolution of salivary gland concoctions. In the absence of the X. cheopis genome sequence, this work serves as an extended reference for the identification of potential pharmacological proteins and peptides present in flea saliva.
MeSH term(s)	Animals ; Chromatography, Liquid ; Female ; Insect Vectors ; Proteomics ; Rats ; Siphonaptera/microbiology ; Siphonaptera/physiology ; Tandem Mass Spectrometry ; Xenopsylla/genetics ; Xenopsylla/microbiology
Language	English
Publishing date	2022-01-04
Publishing country	Netherlands
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, N.I.H., Intramural
ZDB-ID	2400835-7
ISSN	1876-7737 ; 1874-3919
ISSN (online)	1876-7737
ISSN	1874-3919
DOI	10.1016/j.jprot.2021.104476
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Comprehensive Proteomics Analysis of the Hemolymph Composition of Sugar-Fed

Alvarenga, Patricia H / Alves E Silva, Thiago Luiz / Suzuki, Motoshi / Nardone, Glenn / Cecilio, Pedro / Vega-Rodriguez, Joel / Ribeiro, Jose M C / Andersen, John F

Journal of proteome research

2024 Volume 23, Issue 4, Page(s) 1471–1487

Abstract: In arthropods, hemolymph carries immune cells and solubilizes and transports nutrients, hormones, and other molecules that are involved in diverse physiological processes including immunity, metabolism, and reproduction. However, despite such ... ...

Abstract	In arthropods, hemolymph carries immune cells and solubilizes and transports nutrients, hormones, and other molecules that are involved in diverse physiological processes including immunity, metabolism, and reproduction. However, despite such physiological importance, little is known about its composition. We applied mass spectrometry-based label-free quantification approaches to study the proteome of hemolymph perfused from sugar-fed female and male
MeSH term(s)	Humans ; Animals ; Male ; Female ; Aedes/metabolism ; Sugars/metabolism ; Hemolymph/metabolism ; Proteomics ; Carbohydrates
Chemical Substances	Sugars ; Carbohydrates
Language	English
Publishing date	2024-03-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	2078618-9
ISSN	1535-3907 ; 1535-3893
ISSN (online)	1535-3907
ISSN	1535-3893
DOI	10.1021/acs.jproteome.3c00918
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Article: Shuttle-box systems for studying preferred environmental ranges by aquatic animals.

Christensen, Emil A F / Andersen, Lars E J / Bergsson, Heiðrikur / Steffensen, John F / Killen, Shaun S

Conservation physiology

2021 Volume 9, Issue 1, Page(s) coab028

Abstract: Animals' selection of environments within a preferred range is key to understanding their habitat selection, tolerance to stressors and responses to environmental change. For aquatic animals, preferred environmental ranges can be studied in so-called ... ...

Abstract	Animals' selection of environments within a preferred range is key to understanding their habitat selection, tolerance to stressors and responses to environmental change. For aquatic animals, preferred environmental ranges can be studied in so-called shuttle-boxes, where an animal can choose its ambient environment by shuttling between separate choice chambers with differences in an environmental variable. Over time, researchers have refined the shuttle-box technology and applied them in many different research contexts, and we here review the use of shuttle-boxes as a research tool with aquatic animals over the past 50 years. Most studies on the methodology have been published in the latest decade, probably due to an increasing research interest in the effects of environmental change, which underlines the current popularity of the system. The shuttle-box has been applied to a wide range of research topics with regards to preferred ranges of temperature, CO
Language	English
Publishing date	2021-05-17
Publishing country	England
Document type	Journal Article
ZDB-ID	2721508-8
ISSN	2051-1434
ISSN	2051-1434
DOI	10.1093/conphys/coab028
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Validation of ultrasonography for measurement of cartilage thickness in the equine carpus

Andersen, Camilla / Griffin, John F., IV / Jacobsen, Stine / Østergaard, Stine / Walters, Marie / Mori, Yuki / Lindegaard, Casper

Veterinary radiology & ultrasound. 2022 July, v. 63, no. 4

2022

Abstract: Articular cartilage thinning is an important hallmark of osteoarthritis (OA), and ultrasonography (US) is a clinically accessible tool potentially suitable for repeated evaluation. The aim of the present prospective methods comparison study was to ... ...

Abstract	Articular cartilage thinning is an important hallmark of osteoarthritis (OA), and ultrasonography (US) is a clinically accessible tool potentially suitable for repeated evaluation. The aim of the present prospective methods comparison study was to validate US as a tool for measuring cartilage thickness in the carpus of the horse. Eight Standardbred trotters underwent US examination with 9 and 15 MHz linear transducers. Six anatomical locations in the radiocarpal joint (RCJ) and middle carpal joint (MCJ) were examined. The same joints were assessed by ultrahigh field (9.4 Tesla) magnetic resonance imaging (MRI) and histology. Associations between measurements obtained by the different modalities were assessed by ANOVA, Deming regression, Pearson correlation and Bland–Altman plots. Histologically assessed total cartilage thickness (the noncalcified cartilage (NCC) plus the calcified cartilage zone (CCZ)) overestimated thickness compared to MRI (P < 0.01) and US (P < 0.01). US 15 MHz had substantial agreement with MRI and NCC histology, and repeatability was acceptable (coefficient of variation = 8.6–17.9%) when used for assessment of cartilage thickness in the RCJ. In contrast, 9 MHz US showed poorer agreement with MRI and NCC histology, as it overestimated the thickness of thin cartilage and underestimated the thickness of thicker cartilage in the RCJ and MCJ. Moreover, repeatability was suboptimal (coefficient of variation = 10.4–26.3%). A 15 MHz transducer US is recommended for detecting changes in RCJ cartilage thickness or monitoring development over time, and it has the potential for noninvasive assessment of cartilage health in horses.
Keywords	Standardbred ; carpus ; cartilage ; histology ; horses ; joints (animal) ; magnetism ; osteoarthritis ; radiology ; transducers (equipment) ; ultrasonics ; ultrasonography
Language	English
Dates of publication	2022-07
Size	p. 478-489.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	2142058-0
ISSN	1740-8261 ; 1058-8183
ISSN (online)	1740-8261
ISSN	1058-8183
DOI	10.1111/vru.13085
Database	NAL-Catalogue (AGRICOLA)

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