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  1. Article: Studies of coltivirus in China.

    Tao, San-ju / Chen, Bo-quan

    Chinese medical journal

    2005  Volume 118, Issue 7, Page(s) 581–586

    Abstract: Objective: The purpose of this article is to review the developments of studies of Coltivirus in China.: Data sources: The data used in this review was obtained mainly from the studies of Coltivirus reported from 1990 to 2003 in China.: Study ... ...

    Abstract Objective: The purpose of this article is to review the developments of studies of Coltivirus in China.
    Data sources: The data used in this review was obtained mainly from the studies of Coltivirus reported from 1990 to 2003 in China.
    Study selection: Relevant articles on studies of Coltivirus in domestic and foreign literature were selected.
    Data extraction: Data were maily extracted from the articles which are listed in the reference section of this review.
    Results: Many Coltiviruses have been isolated not only from blood samples of patients with unknown fever or from cerebrospinal fluid of patients with encephalitis in Xishuangbanna area in Yunnan province, but also from mosquitoes collected in many areas in China. In some patients diagnosed as Japanese encephalitis or unknown fever, an increase of Coltivirus IgG antibody of fourfold, or more, has been detected using ELISA. Similarly, Coltivirus IgM antibody was positive in some patients with Japanese encephalitis or viral encephalitis. From most Chinese patients, except the northeastern, the isolates of Coltiviruses belong to subgroup B2, according to RT-PCR amplification of the ninth and twelfth segments of the isolates and sequence analysis of their amplicons. Some biological properties of Chinese Coltiviruses isolates are different from that of North American Coltiviruses.
    Conclusions: The isolates of Coltiviruses from Chinese patients are one of the common agents causing viral encephalitis and unknown fever in summer-autumn season. It might be an important public health problem due to its high isolation rate and wide distribution in China. Mosquito is the main transmission vector of the virus.
    MeSH term(s) Animals ; Antibodies, Viral/blood ; Coltivirus/classification ; Coltivirus/genetics ; Coltivirus/immunology ; Coltivirus/isolation & purification ; Genotype ; Humans ; Rats
    Chemical Substances Antibodies, Viral
    Language English
    Publishing date 2005-04-05
    Publishing country China
    Document type Journal Article ; Review
    ZDB-ID 127089-8
    ISSN 0366-6999 ; 1002-0187
    ISSN 0366-6999 ; 1002-0187
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: [Report of 5 cases with Colti virus encephalitis in southeastern areas of Guizhou province].

    Yang, Yu-lin / Wang, Ding-ming / Tang, Xing-qun / Tao, San-ju

    Zhonghua er ke za zhi = Chinese journal of pediatrics

    2004  Volume 42, Issue 11, Page(s) 867–868

    MeSH term(s) China ; Coltivirus ; Encephalitis, Tick-Borne/virology ; Humans
    Language Chinese
    Publishing date 2004-11
    Publishing country China
    Document type Case Reports ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 784523-6
    ISSN 0578-1310
    ISSN 0578-1310
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  3. Article: The effect of bagging on ascorbate in Pyrus fruit

    Zhang, Hu Ping / An Qin / Kai Jie Qi / Shu Tian Tao / Ju You Wu / Xiao San Huang / Shao Ling Zhang

    New Zealand journal of crop and horticultural science. 2019 Jan. 2, v. 47, no. 1

    2019  

    Abstract: Here, the L-ascorbic acid (AsA) content as well as activities of enzymes and genes in AsA biosynthesis were investigated in bagged and unbagged fruit of Pyrus pyrifolia ‘Cuiguan’ during on-tree development. The contents of AsA/dehydroascorbate (DHA) and ... ...

    Abstract Here, the L-ascorbic acid (AsA) content as well as activities of enzymes and genes in AsA biosynthesis were investigated in bagged and unbagged fruit of Pyrus pyrifolia ‘Cuiguan’ during on-tree development. The contents of AsA/dehydroascorbate (DHA) and glutathione (γ-glutamyl-L-cysteinylglycine, GSH)/oxidised glutathione (GSSG) couples, the enzyme activities of L-galactono-1,4-lactone dehydrogenase (GLDH; EC 1.3.2.3), L-galactose dehydrogenase (GalDH; EC 1.1.1.117), ascorbate peroxidase (APX; EC 1.11.1.11), monodehydroascorbate reductase (MDHAR; EC 1.6.5.4), glutathione-dependent dehydroascorbate reductase (DHAR; EC 1.8.5.1) and glutathione reductase (GR; EC 1.6.4.2), and the relative expression levels of GLDH and DHAR genes were highest at 20 days after full bloom (DAFB) and then decreased to their minimum at harvest. When the fruit were grown in the dark induced by bagging, the ascorbate contents, enzyme activities and gene expression amounts had a statistical decline compared to the control in the most sampling dates. However, although L-AsA metabolism was affected and the use of light transmitting fruit bags may be preferable, the drop in vitamin C was not really significant in a dietary sense.
    Keywords Pyrus pyrifolia ; ascorbate peroxidase ; ascorbic acid ; bags ; biosynthesis ; enzyme activity ; flowering ; fruits ; galactose ; gene expression ; genes ; glutathione ; glutathione dehydrogenase (ascorbate) ; glutathione-disulfide reductase ; monodehydroascorbate reductase (NADH)
    Language English
    Dates of publication 2019-0102
    Size p. 19-31.
    Publishing place Taylor & Francis
    Document type Article
    ZDB-ID 2098786-9
    ISSN 1175-8783 ; 0114-0671
    ISSN (online) 1175-8783
    ISSN 0114-0671
    DOI 10.1080/01140671.2018.1462209
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: [Molecular biological identification of Batai virus isolated in China].

    Fu, Shi-hong / Sun, Xiao-hong / Wang, Huan-yu / Cao, Yu-xi / Wang, Huan-qin / Liu, Wei-bin / Tao, San-ju / Liang, Guo-dong

    Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology

    2005  Volume 19, Issue 4, Page(s) 331–334

    Abstract: Background: To study the molecular characteristics of YN92-4 strain isolated from mosquitoes in Yunnan Province and define its classification.: Methods: The S segment of YN92-4 strain was amplified and sequenced by 2 different sets of primers. The ... ...

    Abstract Background: To study the molecular characteristics of YN92-4 strain isolated from mosquitoes in Yunnan Province and define its classification.
    Methods: The S segment of YN92-4 strain was amplified and sequenced by 2 different sets of primers. The phylogenic tree of S fragment was constructed by Phylip bio-software. The amino acid sequences of N and NSs proteins were also studied.
    Results: YN92-4 strain could be amplified by 2 sets of primers respectively, S segment showed a highest homology with Batai virus (X73464), reached 96.4%, the homology of protein N and NSs amio-acid sequence with Batai virus was 99.1% and 98% respectively.
    Conclusion: The YN92-4 strain belongs to Batai virus, this is the first report of molecular biological identification of Batai virus in China.
    MeSH term(s) Amino Acid Sequence ; Animals ; Bunyamwera virus/classification ; Bunyamwera virus/genetics ; Bunyamwera virus/isolation & purification ; China ; Culicidae/virology ; DNA, Complementary/chemistry ; DNA, Complementary/genetics ; Molecular Sequence Data ; Phylogeny ; RNA, Viral/genetics ; RNA, Viral/isolation & purification ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid
    Chemical Substances DNA, Complementary ; RNA, Viral
    Language Chinese
    Publishing date 2005-12
    Publishing country China
    Document type English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1003-9279
    ISSN 1003-9279
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: [Genotyping of the Chinese isolates of coltivirus].

    Xu, Li-hong / Tao, San-ju / Cao, Yu-xi / Wang, Huan-qin / Yang, Dong-rong / He, Ying / Liu, Qin-zhi / Chen, Bo-quan

    Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology

    2003  Volume 17, Issue 4, Page(s) 346–350

    Abstract: Objective: To classify the Chinese isolates of Coltiviruses.: Methods: Three sets of primers were selected among them two were specific to the 9th and 12th segments of subgroup B2, and one was for the 12th segment of subgroup B1-All the Chinese ... ...

    Abstract Objective: To classify the Chinese isolates of Coltiviruses.
    Methods: Three sets of primers were selected among them two were specific to the 9th and 12th segments of subgroup B2, and one was for the 12th segment of subgroup B1-All the Chinese isolates of Coltivirus selected in the experiment were classified according to the lengths of different amplicons of the reverse transcriptase-polymerase Chain reaction (RT-PCR). The homogenicity of the nucleic acids of the isolates BJ95-75 and YN-6 was also compared with other Coltivirus strains belonging to subgroup B2.
    Results: With the primers 12-854-S/12-B2-R, which were specific to the 12th segment of Coltivirus subgroup B2-850 bp amplicons were obtained from Beijing isolate BJ95-75 and all the Yunnan isolates such as YN-6, -67-1, -68-1, -69, -70-1, -70-2, -90, -92-2, -93 of Coltivirus 492 bp DNA fragments were also amplified from all of them with the segment 9th specific primers 9-JKT-S/9-JKT-R. However no positive results were obtained from Northeast isolates NE97-12, NE97-31 and control viruses YN-99(Orbivirus),YN-151-1(JEV) with the same two sets of primers. With 12-B1-S/12-B1R primers specific to the 12th segment of subgroup B1, no amplicons of right length were obtained from any of the Chinese isolates of Coltivirus and the control viruses. When compared the nucleic acid sequences of BJ95-75 and YN-6 with other Coltivirus strains such as Bannavirus, JKT6423, JKT6969, JKT7043, the amplicons from segment 12th of these two strains had more than 89.4% homology with the other strains, especially to the earlier Chinese isolate Bannavirus, the homolog was more then 98.9%. Nearly 96.5% and 99.2% of the nucleic acids of the amplicons from segment 9th of the two strains were being homologous to Bannavirus and about 84.0% to JKT6423, which had been classified into type B2a. But the maximal homogenicity was about 53% when compared with the other two coltivirus strains. JKT6969 and JKT7043 which had been classified into type B2b.
    Conclusion: Genotyping the recent Chinese isolates of coltivirus for the first time in our country. Most of the Chinese isolates belong to subgroup B2, more exactly type B2a. The Northeast isolates NE97-12 and NE97-31 were not correctly grouped with the available primers.
    MeSH term(s) Animals ; Base Sequence ; China ; Coltivirus/classification ; Coltivirus/genetics ; Coltivirus/isolation & purification ; Culicidae/virology ; Genotype ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Nucleic Acid
    Language Chinese
    Publishing date 2003-12
    Publishing country China
    Document type English Abstract ; Journal Article
    ISSN 1003-9279
    ISSN 1003-9279
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: [Isolation and identification of arboviruses in Hebei Province].

    Wang, Huan-qin / Liu, Wei-bing / Yang, Dong-rong / Liang, Yong / Wang, Jun-wei / Zhang, Lian-shan / Liu, Jia-wei / Tao, San-ju / Lv, Xin-jun / Liang, Guo-dong

    Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology

    2006  Volume 20, Issue 1, Page(s) 52–55

    Abstract: Background: To study the arboviruses carried by mosquitoes collected in Hebei Province.: Methods: Samples were collected from mosquito active sites and stored in liquid nitrogen till use. Pools of 20 to 30 mosquitoes were ground after sterilization, ... ...

    Abstract Background: To study the arboviruses carried by mosquitoes collected in Hebei Province.
    Methods: Samples were collected from mosquito active sites and stored in liquid nitrogen till use. Pools of 20 to 30 mosquitoes were ground after sterilization, centrifugal supernant was inoculated onto C6/36 cell, cytopathic effect was observed for three sequential passages. Positive isolates were identified by IFA and RT-PCR.
    Results: Totally 1310 mosquitoes were collected from two villages of She county, Hebei province. They were divided into 46 pools and ground respectively. Thirteen positive isolates were obtained. Two isolates reacted with alphaviral antibodies and were amplified by alphaviral primers, nucleotide sequence showed the highest homology (98%) to Getah virus (AY702913.1), so the two isolates were identified as Getah virus.
    Conclusion: Getah virus was isolated from mosquitoes in Hebei Province. This is the first report of isolating Getah virus from inland of China.
    MeSH term(s) Animals ; Arboviruses/classification ; Arboviruses/genetics ; Arboviruses/isolation & purification ; Cell Line ; Cluster Analysis ; Culicidae/virology ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Analysis, DNA
    Language Chinese
    Publishing date 2006-03
    Publishing country China
    Document type English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1003-9279
    ISSN 1003-9279
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: [Investigation of Arboviruses in Lancang river down-stream area in Yunnan province].

    Tao, San-ju / Zhang, Hai-lin / Yang, Dong-rong / Wang, Huan-qin / Liu, Qin-zhi / Zhang, Yun-zhi / Yang, Wei-hong / Zhang, Yu-zhen / Cao, Yu-xi / Xu, Li-hong / He, Ying / Chen, Bo-quan

    Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology

    2003  Volume 17, Issue 4, Page(s) 322–326

    Abstract: Objective: To investigate the epidemic state of arboviruses in the downstream area of Lancang river in Yunnan province.: Methods: Mosquitoes were collected from Lancang river downstream area (including Lancang county and Simao city) during summer in ... ...

    Abstract Objective: To investigate the epidemic state of arboviruses in the downstream area of Lancang river in Yunnan province.
    Methods: Mosquitoes were collected from Lancang river downstream area (including Lancang county and Simao city) during summer in 1998 and stored in liquid nitrogen after classification. The mosquito pools were homogenized and centrifuged, then the supernatant was inoculated into C6/36 cells for virus isolation. New isolates were identified by neutralization test(NT), ELISA, immunofluorescence assay(IFA) and polyacrylamid gel electrophoresis(PAGE).
    Results: Totally 22 isolates of arbovirus were obtained from 233 mosquito pools by inoculation of C6/36 cells and positive rate of the isolation was 9.4%. Ten strains were resistant to both ether and 5 prime-IDU. So they were non-enveloped double-stranded (ds) RNA virus. Twelve segmented RNAs were shown by PAGE and PAGE profiles from the ten strains were 6-6 with minor variation. The isolates can be neutralized by immunized mouse ascites fluid of BJ95-75 strains of coltivirus by NT, and reacted with monoclonal antibody against BJ95-75 by ELISA. These viruses were identified as coltivirus. Nine isolates were sensitive to ether and resistant to 5 prime-IDU. So they were non-enveloped RNA viruses. PAGE showed 10 segmented RNA, and they were identified to be orbiviruses. Three isolations were sensitive to ether. One of them can be neutralized with JEV A2 strain antibody by NT and was positive to the homologous antibody by IFA. It was identified being strain of JE virus. One strain(YN92-4) can be reacted with anti-bunyavirus group specific immune ascites fluid by both IFA and ElISA, but reacted neither with anti-alpha virus group, nor with anti-flavivirus group JE virus ascites fluid. The virions are spherical and about 87 nm in diameter with surface projections by negative staining. Conclusion Twenty-two isolates have been obtained from wild caught-mosquitoes of Lancang river down-stream area in Yunnan province. Among them ten, nine, one and one were identified as coltivirus, orbivirus, JE virus and bunyavirus, respectively. One is under identification. This is the first report on bunyavirus isolated from mosquitoes in China.
    MeSH term(s) Animals ; Arboviruses/classification ; Arboviruses/isolation & purification ; China ; Coltivirus/isolation & purification ; Culicidae/virology ; Encephalitis Virus, Japanese/isolation & purification ; Insect Vectors/virology ; Orbivirus/isolation & purification ; Orthobunyavirus/isolation & purification
    Language Chinese
    Publishing date 2003-12
    Publishing country China
    Document type English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1003-9279
    ISSN 1003-9279
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: [Relation between bone matrix proteins and monocyte chemoattractant protein-1 in renal small artery in diabetic nephropathy rats].

    Zhao, An-ju / Huang, Song-min / Ou, San-tao / Liu, Fang / Chen, Ze-jun / Hu, Zhang-xue / Tang, Wan-xin

    Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition

    2012  Volume 43, Issue 3, Page(s) 414–419

    Abstract: Objective: To observe the expressions of bone matrix proteins and monocyte chemoattractant protein-1 ((MCP-1) in the renal arteriole of diabetic nephropathy (DN) rats and analyze their correlations and roles in diabetic nephropathy.: Methods: Adult ... ...

    Abstract Objective: To observe the expressions of bone matrix proteins and monocyte chemoattractant protein-1 ((MCP-1) in the renal arteriole of diabetic nephropathy (DN) rats and analyze their correlations and roles in diabetic nephropathy.
    Methods: Adult Sprague-Dawley male rats were used to establish the animal model of diabetic nephropathy induced by peritoneal injection of 55 mg/kg of streptozocin. Calcium deposit around the renal arteriole was observed by alizarin red staining. The protein and mRNA levels of core-bind factor alpha 1 (cbfalpha1), bone morphogenetic protein 2 (BMP-2) and matrix Gla protein (MGP) in renal arteriole of DN rats were detected by immunohistochemistry, in-situ hybridization and real-time PCR. The biochemical indices were detected by routine test.
    Results: 1. Blood glucose and Urine protein of 24 h were significantly increased in the renal arteriole of DN rats versus the control rats (P < 0.05), serum creatinine (SCr) and phosphorus were significantly increased from 12 weeks. 2. Little deposit of calcium salt was observed in the renal arteriole of DN rats at the 4th week and a large amount of deposit was observed at 24th week, but no calcium deposit was observed in control rats. 3. Cbfalpha1 and BMP-2 expressions were significantly increased in the renal arteriole of DN rats from 4 to 24 weeks vs. the control rats. MGP mRNA expression in the renal arteriole of DN rats was significantly decreased from 4 to 24 weeks. MCP-1 expression was obviously upregulated in the renal arteriole of DN rats at 24th week versus that at 4th and 12th week. No MCP-1 expression was observed in the renal arterioles of control rats. MCP-1 were positively correlated with the expression of cbfalpha1 and BMP-2.
    Conclusion: Bone matrix proteins has already expressed in renal arteriole before the formation of vascular calcification. MCP-1 can affect the expression of cbfalpha1, BMP-2; cbfalpha1, BMP-2, MGP and MCP-1 may be involved in the formation of vascular lesions of DN.
    MeSH term(s) Animals ; Arterioles/metabolism ; Bone Matrix/metabolism ; Bone Morphogenetic Protein 2/metabolism ; Calcium-Binding Proteins/metabolism ; Chemokine CCL2/metabolism ; Core Binding Factor Alpha 1 Subunit/metabolism ; Diabetic Nephropathies/metabolism ; Extracellular Matrix Proteins/metabolism ; Kidney/blood supply ; Male ; Rats ; Rats, Sprague-Dawley ; Matrix Gla Protein
    Chemical Substances Bmp2 protein, rat ; Bone Morphogenetic Protein 2 ; Calcium-Binding Proteins ; Ccl2 protein, rat ; Chemokine CCL2 ; Core Binding Factor Alpha 1 Subunit ; Extracellular Matrix Proteins ; Runx2 protein, rat
    Language Chinese
    Publishing date 2012-07-19
    Publishing country China
    Document type English Abstract ; Journal Article
    ZDB-ID 2106840-9
    ISSN 1672-173X
    ISSN 1672-173X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: PET imaging of serotonin transporters with 4-[18F]-ADAM in a Parkinsonian rat model.

    Weng, Shao-Ju / Shiue, Chyng-Yann / Huang, Wen-Sheng / Cheng, Cheng-Yi / Huang, San-Yuan / Li, I-Hsun / Tao, Chih-Chieh / Chou, Ta-Kai / Liao, Mei-Hsiu / Chang, Yung-Ping / Ma, Kuo-Hsing

    Cell transplantation

    2013  Volume 22, Issue 7, Page(s) 1295–1305

    Abstract: This study was undertaken to address the effects of fetal mesencephalic tissue transplantation on the serotonin system in a rat model of Parkinson's disease (PD) while also investigating the usefulness of 4-[18F]-ADAM (a serotonin transporter imaging ... ...

    Abstract This study was undertaken to address the effects of fetal mesencephalic tissue transplantation on the serotonin system in a rat model of Parkinson's disease (PD) while also investigating the usefulness of 4-[18F]-ADAM (a serotonin transporter imaging agent) coupled with micro-PET for imaging serotonin transporters (SERTs). A PD model was induced by unilateral injection of 6-hydroxydopamine (6-OHDA) into the right medial forebrain bundle of the nigrostriatal pathway, while cell transplantation was performed via intrastriatal injection of mesencephalic brain tissue dissected from embryonic (E14) rats. The 4-[18F]-ADAM/micro-PET scanning was performed following both 6-OHDA lesioning and transplantation. Immunohistochemistry (IHC) studies were also performed following the final PET scan, and the results were compared to show a 17-43% decrease in the specific uptake ratio (SUR) and a 23-52% decrease in serotonin transporter immunoreactivity (SERT-ir) within various brain regions on the lesioned side. The number of methamphetamine-induced rotations also decreased significantly at the 4th week postgraft. In addition, striatal SUR and the SERT-ir levels were restored to 77% and 83% 5 weeks postgraft. These results suggest that Parkinson's disease also affects the serotonergic system, while both the dopaminergic and serotonergic systems can be partially restored in a rat model of PD after E14 mesencephalic tissue transplantation. In addition, we have also determined that 4-[18F]-ADAM/micro-PET can be used to detect serotonergic neuron loss, monitor the progress of Parkinson's disease, and oversee the effectiveness of therapy.
    MeSH term(s) ADAM Proteins/chemistry ; ADAM Proteins/metabolism ; ADAM Proteins/pharmacology ; Animals ; Brain/diagnostic imaging ; Brain/drug effects ; Disease Models, Animal ; Dopaminergic Neurons/physiology ; Fluorine Radioisotopes/chemistry ; Immunohistochemistry ; Male ; Mesencephalon/cytology ; Mesencephalon/transplantation ; Odds Ratio ; Oxidopamine/pharmacology ; Parkinson Disease/diagnostic imaging ; Parkinson Disease/metabolism ; Parkinson Disease/pathology ; Positron-Emission Tomography ; Rats ; Rats, Sprague-Dawley ; Serotonergic Neurons/physiology ; Serotonin Plasma Membrane Transport Proteins/metabolism ; Transplantation, Homologous
    Chemical Substances Fluorine Radioisotopes ; Serotonin Plasma Membrane Transport Proteins ; Oxidopamine (8HW4YBZ748) ; ADAM Proteins (EC 3.4.24.-)
    Language English
    Publishing date 2013
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1135816-6
    ISSN 1555-3892 ; 0963-6897
    ISSN (online) 1555-3892
    ISSN 0963-6897
    DOI 10.3727/096368912X658683
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: PET Imaging of Serotonin Transporters with 4-[F]-ADAM in a Parkinsonian Rat Model

    Shao-Ju Weng / Chyng-Yann Shiue / Wen-Sheng Huang / Cheng-Yi Cheng / San-Yuan Huang / I-Hsun Li / Chih-Chieh Tao / Ta-Kai Chou / Mei-Hsiu Liao / Yung-Ping Chang / Kuo-Hsing Ma Ph.D.

    Cell Transplantation, Vol

    2013  Volume 22

    Abstract: This study was undertaken to address the effects of fetal mesencephalic tissue transplantation on the serotonin system in a rat model of Parkinson's disease (PD) while also investigating the usefulness of 4-[ 18 F]-ADAM (a serotonin transporter imaging ... ...

    Abstract This study was undertaken to address the effects of fetal mesencephalic tissue transplantation on the serotonin system in a rat model of Parkinson's disease (PD) while also investigating the usefulness of 4-[ 18 F]-ADAM (a serotonin transporter imaging agent) coupled with micro-PET for imaging serotonin transporters (SERTs). A PD model was induced by unilateral injection of 6-hydroxydopamine (6-OHDA) into the right medial forebrain bundle of the nigrostriatal pathway, while cell transplantation was performed via intrastriatal injection of mesencephalic brain tissue dissected from embryonic (E14) rats. The 4-[ 18 F]-ADAM/micro-PET scanning was performed following both 6-OHDA lesioning and transplantation. Immunohistochemistry (IHC) studies were also performed following the final PET scan, and the results were compared to show a 17–43% decrease in the specific uptake ratio (SUR) and a 23–52% decrease in serotonin transporter immunoreactivity (SERT-ir) within various brain regions on the lesioned side. The number of methamphetamine-induced rotations also decreased significantly at the 4th week postgraft. In addition, striatal SUR and the SERT-ir levels were restored to 77% and 83% 5 weeks postgraft. These results suggest that Parkinson's disease also affects the serotonergic system, while both the dopaminergic and serotonergic systems can be partially restored in a rat model of PD after E14 mesencephalic tissue transplantation. In addition, we have also determined that 4-[ 18 F]-ADAM/micro-PET can be used to detect serotonergic neuron loss, monitor the progress of Parkinson's disease, and oversee the effectiveness of therapy.
    Keywords Medicine ; R
    Subject code 610
    Language English
    Publishing date 2013-07-01T00:00:00Z
    Publisher SAGE Publishing
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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