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Article ; Online: Antihyperglycemic and antihyperlipidemic activities of wild musk melon (

Gopalasatheeskumar, K / Ariharasivakumar, G / Kalaichelvan, V K / Sengottuvel, T / Devan, V Sanish / Srividhya, V

Chinese herbal medicines

2020 Volume 12, Issue 4, Page(s) 399–405

Abstract: Objective: Wild musk melon (: Methods: Diabetes was induced by administration of STZ (60 mg/kg, i.p.) after 15 min of NIC (120 mg/kg i.p.) administration. The diabetic rats were treated with HALEC (300 and 600 mg/kg, p.o., respectively) for 21 d.: ... ...

Abstract	Objective: Wild musk melon ( Methods: Diabetes was induced by administration of STZ (60 mg/kg, i.p.) after 15 min of NIC (120 mg/kg i.p.) administration. The diabetic rats were treated with HALEC (300 and 600 mg/kg, p.o., respectively) for 21 d. Results: After the management with HALEC, blood glucose, HbA1c levels, total cholesterol, LDL cholesterol, triglycerides levels, glycogen phosphorylase and glucose-6-phosphatase levels were significantly diminished in diabetic rats. However, haemoglobin level, HDL cholesterol, liver glycogen, total protein, hexokinase, glucose-6-phosphate dehydrogenase levels were significantly increased in HALEC treated diabetic rats. The histopathological studies of the pancreas in HALEC-treated diabetic rats showed almost normal appearance. L6 cell line study revealed the increased glucose uptake activity of HALEC. High performance thin layer chromatography (HPTLC) analysis confirms the presence of active principles such as rutin, gallic acid and quercetin in HALEC. Conclusion: The results indicated that HALEC possess significant antihyperglycemic and antihyperlipidemic activity in STZ-NIC-induced type II diabetic rats with protective effect. This research work will be useful for the isolation of active principles and development of herbal formulation in phytopharmaceuticals.
Language	English
Publishing date	2020-09-04
Publishing country	Singapore
Document type	Journal Article
ISSN	2589-3610
ISSN (online)	2589-3610
DOI	10.1016/j.chmed.2020.02.005
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Bioactive Amento flavone isolated from Cassia fistula L. leaves exhibits therapeutic efficacy.

Srividhya, M / Hridya, H / Shanthi, V / Ramanathan, K

3 Biotech

2017 Volume 7, Issue 1, Page(s) 33

Abstract: Novel natural compounds endowed with sound bioactivities are currently the utmost need as leads toward drug discovery. For the first time, here, we report the presence of Amentoflavone (biflavonoid) in the leaves of Cassia fistula L. Structural ... ...

Abstract	Novel natural compounds endowed with sound bioactivities are currently the utmost need as leads toward drug discovery. For the first time, here, we report the presence of Amentoflavone (biflavonoid) in the leaves of Cassia fistula L. Structural characterization was carried out using ultraviolet-visible spectrophotometer, Fourier transform infrared, nuclear magnetic resonance, and thin-layer chromatography. The isolated compound was further evaluated for its bioactivity. The compound demonstrated moderate cytotoxicity in liver carcinoma (HepG2) cells, and the comparative analysis for the standard and normal compound has also been validated. Antioxidant potential was assessed by DPPH assay. Furthermore, efficacy of the compound in the aforesaid assays asserts its bioactivity and subsequently its importance as a potent therapeutic. Our study strongly suggests that Amentoflavone present in the leaf extracts of C. fistula L. definitely holds promise in the pharmaceutical industry.
Language	English
Publishing date	2017-04-11
Publishing country	Germany
Document type	Case Reports
ZDB-ID	2600522-0
ISSN	2190-5738 ; 2190-572X
ISSN (online)	2190-5738
ISSN	2190-572X
DOI	10.1007/s13205-017-0599-7
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Article: Bioactive Amento flavone isolated from Cassia fistula L. leaves exhibits therapeutic efficacy

Srividhya, M / Hridya, H / Shanthi, V / Ramanathan, K

3 Biotech. 2017 May, v. 7, no. 1

2017

Abstract	Novel natural compounds endowed with sound bioactivities are currently the utmost need as leads toward drug discovery. For the first time, here, we report the presence of Amentoflavone (biflavonoid) in the leaves of Cassia fistula L. Structural characterization was carried out using ultraviolet–visible spectrophotometer, Fourier transform infrared, nuclear magnetic resonance, and thin-layer chromatography. The isolated compound was further evaluated for its bioactivity. The compound demonstrated moderate cytotoxicity in liver carcinoma (HepG2) cells, and the comparative analysis for the standard and normal compound has also been validated. Antioxidant potential was assessed by DPPH assay. Furthermore, efficacy of the compound in the aforesaid assays asserts its bioactivity and subsequently its importance as a potent therapeutic. Our study strongly suggests that Amentoflavone present in the leaf extracts of C. fistula L. definitely holds promise in the pharmaceutical industry.
Keywords	2,2-diphenyl-1-picrylhydrazyl ; Cassia fistula ; antioxidant activity ; carcinoma ; cytotoxicity ; drugs ; flavones ; leaf extracts ; leaves ; liver ; nuclear magnetic resonance spectroscopy ; pharmaceutical industry ; spectrophotometers ; thin layer chromatography
Language	English
Dates of publication	2017-05
Size	p. 33.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	2600522-0
ISSN	2190-5738 ; 2190-572X
ISSN (online)	2190-5738
ISSN	2190-572X
DOI	10.1007/s13205-017-0599-7
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Article: Characterization of DLP12 Prophage Membrane Associated Protein: HolinGFP.

Srividhya, K V / Krishnaswamy, S

Indian journal of microbiology

2012 Volume 52, Issue 3, Page(s) 464–471

Abstract: Lysis cassette genes from phages determine the final lytic event of the host cells. The lysis cassette genes are conserved in phages and prophages. The membrane associated holin from DLP12 prophage, available as a GFP fusion construct, was shown to be ... ...

Abstract	Lysis cassette genes from phages determine the final lytic event of the host cells. The lysis cassette genes are conserved in phages and prophages. The membrane associated holin from DLP12 prophage, available as a GFP fusion construct, was shown to be overexpressed, using confocal microscopy analysis, in bacterial cells. The protein expression caused cell death in E. coli AG1 strain suggesting the protein was functional. The His-tag HolinGFP protein was purified using cobalt affinity column and was eluted in the presence of different non-ionic detergents DDM (n-dodecyl-ß-d-maltoside), LDAO (Lauryldimethylamine-oxide), OG (n-octyl β-d-glucopyranoside) and C12E9 (dodecyl nonaoxyethylene ether). HolinGFP existed predominantly as a dimer in LDAO in Superdex S200 gel filtration chromatography. Circular dichroism and fluorescence spectroscopy of the fluorescent HolinGFP in all four detergents (C12E9, DDM, LDAO, and OG) confirmed the folded state. Both dithiobis succinimidyl propionate and gluteraldehyde crosslinking revealed the existence of higher order oligomers and dimers. HolinGFP has been functionally and biophysically characterised and is being explored for crystallographic structure determination.
Language	English
Publishing date	2012-06-28
Publishing country	India
Document type	Journal Article
ZDB-ID	413422-9
ISSN	0973-7715 ; 0046-8991
ISSN (online)	0973-7715
ISSN	0046-8991
DOI	10.1007/s12088-012-0278-7
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Article: Genetic Variability Studies of Yellow Mosaic Virus Infecting Blackgram [Vigna mungo (L.) Hepper] from Andhra Pradesh, India

Vijay Kumar Naik, D. / Bhaskara Reddy, B. V. / Sarada Jayalakshmi Devi, R. / Prasanthi, L. / Lakshminarayana Vemireddy, R. / Srividhya, Akkari / Chaithanya, B. H. / Hemalatha, T. M. / Sailaja, K.

Legume research

2022 Volume 45, Issue 7, Page(s) 907

Document type	Article
ZDB-ID	407856-1
ISSN	0250-5371
Database	Current Contents Nutrition, Environment, Agriculture

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Article: Information theoretic perspective on genome clustering.

Veluchamy, Alaguraj / Mehta, Preeti / Srividhya, K V / Vikram, Hirendra / Govind, M K / Gupta, Ramneek / Aziz Bin Dukhyil, Abdul / Abdullah Alharbi, Raed / Abdullah Aloyuni, Saleh / Hassan, Mohamed M / Krishnaswamy, S

Saudi journal of biological sciences

2020 Volume 28, Issue 3, Page(s) 1867–1889

Abstract: Shannon's information theoretic perspective of communication helps one to understand the storage and processing of information in one-dimensional sequences. An information theoretic analysis of 937 available completely sequenced prokaryotic genomes and ... ...

Abstract	Shannon's information theoretic perspective of communication helps one to understand the storage and processing of information in one-dimensional sequences. An information theoretic analysis of 937 available completely sequenced prokaryotic genomes and 238 eukaryotic chromosomes is presented. Information content (Id) values were used to cluster these chromosomes. Chargaff's second parity rule i.e compositional self-complementarity, an empirical fact is observed in all the genomes, except for the proteobacteria
Language	English
Publishing date	2020-12-31
Publishing country	Saudi Arabia
Document type	Journal Article
ZDB-ID	2515206-3
ISSN	2213-7106 ; 1319-562X
ISSN (online)	2213-7106
ISSN	1319-562X
DOI	10.1016/j.sjbs.2020.12.039
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Article: Subclassification and targeted characterization of prophage-encoded two-component cell lysis cassette.

Srividhya, K V / Krishnaswamy, S

Journal of biosciences

2007 Volume 32, Issue 5, Page(s) 979–990

Abstract: Bacteriophage induced lysis of host bacterial cell is mediated by a two component cell lysis cassette comprised of holin and lysozyme. Prophages are integrated forms of bacteriophages in bacterial genomes providing a repertoire for bacterial evolution. ... ...

Abstract	Bacteriophage induced lysis of host bacterial cell is mediated by a two component cell lysis cassette comprised of holin and lysozyme. Prophages are integrated forms of bacteriophages in bacterial genomes providing a repertoire for bacterial evolution. Analysis using the prophage database (http://bicmku.in:8082) constructed by us showed 47 prophages were associated with putative two component cell lysis genes. These proteins cluster into four different subgroups. In this process, a putative holin (essd) and endolysin (ybcS), encoded by the defective lambdoid prophage DLP12 was found to be similar to two component cell lysis genes in functional bacteriophages like p21 and P1. The holin essd was found to have a characteristic dual start motif with two transmembrane regions and C-terminal charged residues as in class II holins. Expression of a fusion construct of essd in Escherichia coli showed slow growth. However, under appropriate conditions, this protein could be over expressed and purified for structure function studies. The second component of the cell lysis cassette, ybcS, was found to have an N-terminal SAR (Signal Arrest Release) transmembrane domain. The construct of ybcS has been over expressed in E.coli and the purified protein was functional, exhibiting lytic activity against E.coli and Salmonella typhi cell wall substrate. Such targeted sequence- structure-function characterization of proteins encoded by cryptic prophages will help understand the contribution of prophage proteins to bacterial evolution.
MeSH term(s)	Amino Acid Sequence ; Bacteriolysis/genetics ; Endopeptidases/genetics ; Endopeptidases/metabolism ; Endopeptidases/physiology ; Gene Expression Profiling ; Lysogeny/genetics ; Molecular Sequence Data ; Prophages/chemistry ; Prophages/classification ; Prophages/genetics ; Prophages/pathogenicity
Chemical Substances	Endopeptidases (EC 3.4.-) ; endolysin (EC 3.4.99.-)
Language	English
Publishing date	2007-10-03
Publishing country	India
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	756157-x
ISSN	0250-5991
ISSN	0250-5991
DOI	10.1007/s12038-007-0097-x
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Article ; Online: Information theoretic perspective on genome clustering

Alaguraj Veluchamy / Preeti Mehta / K.V. Srividhya / Hirendra Vikram / M.K. Govind / Ramneek Gupta / Abdul Aziz Bin Dukhyil / Raed Abdullah Alharbi / Saleh Abdullah Aloyuni / Mohamed M. Hassan / S. Krishnaswamy

Saudi Journal of Biological Sciences, Vol 28, Iss 3, Pp 1867-

2021 Volume 1889

Abstract: Shannon’s information theoretic perspective of communication helps one to understand the storage and processing of information in one-dimensional sequences. An information theoretic analysis of 937 available completely sequenced prokaryotic genomes and ... ...

Abstract	Shannon’s information theoretic perspective of communication helps one to understand the storage and processing of information in one-dimensional sequences. An information theoretic analysis of 937 available completely sequenced prokaryotic genomes and 238 eukaryotic chromosomes is presented. Information content (Id) values were used to cluster these chromosomes. Chargaff’s second parity rule i.e compositional self-complementarity, an empirical fact is observed in all the genomes, except for the proteobacteria Candidatus Hodgkinia cicadicola. High information content, arising out of biased base composition in all the 14 chromosomes of Plasmodium falciparum is found among two other genomes of prokaryotes viz. Buchnera aphidicola str. Cc (Cinara cedri) and Candidatus Carsonella ruddii PV. Despite size and compositional variations, both prokaryotic and eukaryotic genomes do not deviate significantly from an equiprobable and random situation. Eukaryotic chromosomes of an organism tend to have similar informational restraints as seen when a simple distance based method is used to cluster them. In eukaryotes, in certain cases, Id values are also similar for the two arms (p and q arm) of the chromosomes. The results of this current study confirm that the information content can provide insights into the clustering of genomes and the evolution of messaging strategies of the genomes. An efficient and robust Perl CGI standalone tool is created based on this information theory algorithm for the analysis of the whole genomes and is made available at https://github.com/AlagurajVeluchamy/InformationTheory.
Keywords	Information theory ; Genome evolution ; Genome clustering ; Nucleotide distribution ; Shannon redundancy ; Genome arrangement ; Biology (General) ; QH301-705.5
Subject code	302
Language	English
Publishing date	2021-03-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Genetic Variability Studies of Yellow Mosaic Virus Infecting Blackgram [Vigna mungo (L.) Hepper] from Andhra Pradesh, India

Naik, D. Vijay Kumar / Reddy, B.V. Bhaskara / Devi, R. Sarada Jayalakshmi / Prasanthi, L. / Vemireddy, R. Lakshminarayana / Srividhya, Akkari / Chaithanya, B.H. / Hemalatha, T.M. / Sailaja, K.

Legume research. 2022 July, v. 45, no. 7

2022

Abstract: The productivity of blackgram is affected by many biotic and abiotic stresses. Among the biotic stresses, yellow mosaic disease (YMD) cause severe yield loss and it is caused by four distinct viruses (belongs to genus begomovirus) collectively known as ... ...

Abstract	The productivity of blackgram is affected by many biotic and abiotic stresses. Among the biotic stresses, yellow mosaic disease (YMD) cause severe yield loss and it is caused by four distinct viruses (belongs to genus begomovirus) collectively known as yellow mosaic virus (YMV). Hence there is need to characterize various YMV isolates associated with blackgram in Andhra Pradesh. YMV infected blackgram samples were collected from East Godavari, Kurnool and Prakasam districts of Andhra Pradesh. The Rolling Circle Amplification (RCA) based full length MYMIV DNA-A and MYMV DNA-B of three isolates were cloned and sequenced. Nucleotide sequence of full length DNA-A of MYMIV-East Godavari isolate showed >96% similarity at nucleotide and >90% at amino acid level with other MYMIV isolates in NCBI database. The complete DNA-A nucleotide sequence of MYMIV-Kurnool and MYMIV-Prakasam isolates shared >99% similarity at nucleotide and >98% at amino acid level with other MYMIV isolates. The complete nucleotide sequence of MYMV DNA-B of three isolates (East Godavari, Kurnool and Prakasam) had >97% homology with other MYMV DNA-B isolates from database. The predicted amino acid sequence of MYMV DNA-B of three isolates shared >96% homology with other MYMV-B isolates. The common region (CR) sequence similarity between MYMIV-As and MYMV-Bs of East Godavari and Kurnool isolates was 76.4% and 78.3% with Prakasam isolate. The divergence between the MYMIV-A and MYMV-B of present three isolates (East Godavari, Kurnool and Prakasam) under study were ranged from 22.2 to 22.6%.
Keywords	Begomovirus ; Vigna mungo ; amino acid sequences ; amino acids ; black gram ; databases ; genetic variation ; nucleotide sequences ; research ; sequence homology ; viruses ; India
Language	English
Dates of publication	2022-07
Size	p. 907-913.
Publishing place	Agricultural Research Communication Centre
Document type	Article
ISSN	0976-0571
DOI	10.18805/LR-4800
Database	NAL-Catalogue (AGRICOLA)

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Article: Sub classification and targeted characterization of prophage-encoded two-component cell lysis cassette

Srividhya, K. V / Krishnaswamy, S

Journal of biosciences. 2007 Aug., v. 32, no. Supplement 1

2007

Abstract	Bacteriophage induced lysis of host bacterial cell is mediated by a two component cell lysis cassette comprised of holin and lysozyme. Prophages are integrated forms of bacteriophages in bacterial genomes providing a repertoire for bacterial evolution. Analysis using the prophage database (http://bicmku.in:8082) constructed by us showed 47 prophages were associated with putative two component cell lysis genes. These proteins cluster into four different subgroups. In this process, a putative holin (essd) and endolysin (ybcS), encoded by the defective lambdoid prophage DLP12 was found to be similar to two component cell lysis genes in functional bacteriophages like p21 and P1. The holin essd was found to have a characteristic dual start motif with two transmembrane regions and C-terminal charged residues as in class II holins. Expression of a fusion construct of essd in Escherichia coli showed slow growth. However, under appropriate conditions, this protein could be over expressed and purified for structure function studies. The second component of the cell lysis cassette, ybcS, was found to have an N-terminal SAR (Signal Arrest Release) transmembrane domain. The construct of ybcS has been over expressed in E. coli and the purified protein was functional, exhibiting lytic activity against E. coli and Salmonella typhi cell wall substrate. Such targeted sequence-structure-function characterization of proteins encoded by cryptic prophages will help understand the contribution of prophage proteins to bacterial evolution.
Keywords	Escherichia coli ; Salmonella Typhi ; bacteria ; bacteriophages ; cell walls ; databases ; endolysin ; evolution ; genes ; lysozyme ; proteins
Language	English
Dates of publication	2007-08
Size	p. 979-990.
Publishing place	Springer-Verlag
Document type	Article
ZDB-ID	756157-x
ISSN	0973-7138 ; 0250-5991
ISSN (online)	0973-7138
ISSN	0250-5991
DOI	10.1007/s12038-007-0097-x
Database	NAL-Catalogue (AGRICOLA)

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