LIVIVO - Search results -

Search results

Result 1 - 10 of total 55

Search options

Article: How a Proposed Hypothesis during My PhD Training Shaped My Career.

Sadegh-Nasseri, Scheherazade

2021 Volume 40, Issue 5, Page(s) 449–464

Abstract: In this memoir-style essay, I have narrated the evolution of my scientific career, as deeply influenced by my PhD training and the mentorship of Professor Eli Sercarz. Starting in his lab, and continuing to my own laboratory, many of the questions we ... ...

Abstract	In this memoir-style essay, I have narrated the evolution of my scientific career, as deeply influenced by my PhD training and the mentorship of Professor Eli Sercarz. Starting in his lab, and continuing to my own laboratory, many of the questions we have pursued link in some way to Eli's ideas. In this essay, I have summarized the path that I followed after graduating from his lab and highlight findings along the way. I apologize to my colleagues whose work was not discussed here due to the nature of this review and space limitations.
Language	English
Publishing date	2021-01-14
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural
ZDB-ID	1353116-5
ISSN	1040-8401
ISSN	1040-8401
DOI	10.1615/CritRevImmunol.2020035324
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1699: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Partnering for the major histocompatibility complex class II and antigenic determinant requires flexibility and chaperons.

Sadegh-Nasseri, Scheherazade

Current opinion in immunology

2021 Volume 70, Page(s) 112–121

Abstract: Cytotoxic, or helper T cells recognize antigen via T cell receptors (TCRs) that can see their target antigen as short sequences of peptides bound to the groove of proteins of major histocompatibility complex (MHC) class I, and class II respectively. For ... ...

Abstract	Cytotoxic, or helper T cells recognize antigen via T cell receptors (TCRs) that can see their target antigen as short sequences of peptides bound to the groove of proteins of major histocompatibility complex (MHC) class I, and class II respectively. For MHC class II epitope selection from exogenous pathogens or self-antigens, participation of several accessory proteins, molecular chaperons, processing enzymes within multiple vesicular compartments is necessary. A major contributing factor is the MHC class II structure itself that uniquely offers a dynamic and flexible groove essential for epitope selection. In this review, I have taken a historical perspective focusing on the flexibility of the MHC II molecules as the driving force in determinant selection and interactions with the accessory molecules in antigen processing, HLA-DM and HLA-DO.
MeSH term(s)	Animals ; Antigen Presentation/immunology ; Epitopes/immunology ; Histocompatibility Antigens Class II/immunology ; Humans ; Molecular Chaperones/immunology
Chemical Substances	Epitopes ; Histocompatibility Antigens Class II ; Molecular Chaperones
Language	English
Publishing date	2021-06-17
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	1035767-1
ISSN	1879-0372 ; 0952-7915
ISSN (online)	1879-0372
ISSN	0952-7915
DOI	10.1016/j.coi.2021.05.005
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2773: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 13: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Proper development of long-lived memory CD4 T cells requires HLA-DO function.

Song, Nianbin / Welsh, Robin A / Sadegh-Nasseri, Scheherazade

Frontiers in immunology

2023 Volume 14, Page(s) 1277609

Abstract: Introduction: HLA-DO (DO) is an accessory protein that binds DM for trafficking to MIIC and has peptide editing functions. DO is mainly expressed in thymic medulla and B cells. Using biochemical experiments, our lab has discovered that DO has ... ...

Abstract	Introduction: HLA-DO (DO) is an accessory protein that binds DM for trafficking to MIIC and has peptide editing functions. DO is mainly expressed in thymic medulla and B cells. Using biochemical experiments, our lab has discovered that DO has differential effects on editing peptides of different sequences: DO increases binding of DM-resistant peptides and reduces the binding of DM-sensitive peptides to the HLA-DR1 molecules. In a separate line of work, we have established that appropriate densities of antigen presentation by B cells during the contraction phase of an infection, induces quiescence in antigen experienced CD4 T cells, as they differentiate into memory T cells. This quiescence phenotype helps memory CD4 T cell survival and promotes effective memory responses to secondary Ag challenge. Methods: Based on our mechanistic understanding of DO function, it would be expected that if the immunodominant epitope of antigen is DM-resistant, presentation of decreased densities of pMHCII by B cells would lead to faulty development of memory CD4 T cells in the absence of DO. We explored the effects of DO on development of memory CD4 T cells and B cells utilizing two model antigens, H5N1-Flu Ag bearing DM-resistant, and OVA protein, which has a DM-sensitive immunodominant epitope and four mouse strains including two DO-deficient Tg mice. Using Tetramers and multiple antibodies against markers of memory CD4 T cells and B cells, we tracked memory development. Results: We found that immunized DR1 Conclusion: These results demonstrate that in the absence of DO, the presentation of cognate foreign antigens in the DO-KO mice is altered and can impact the proper development of memory cells. These findings provide new insights on vaccination design leading to better immune memory responses.
MeSH term(s)	Animals ; Mice ; CD4-Positive T-Lymphocytes ; Immunodominant Epitopes ; Influenza A Virus, H5N1 Subtype/metabolism ; Memory T Cells ; Peptides
Chemical Substances	Immunodominant Epitopes ; Peptides ; H-2O antigen
Language	English
Publishing date	2023-10-16
Publishing country	Switzerland
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2023.1277609
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Editorial overview: Nature repeating itself: conformational flexibility determines MHC class I and class II epitope selection.

Sadegh-Nasseri, Scheherazade / Springer, Sebastian

Current opinion in immunology

2021 Volume 70, Page(s) iii–iv

MeSH term(s)	Animals ; Histocompatibility Antigens Class I/chemistry ; Histocompatibility Antigens Class I/immunology ; Histocompatibility Antigens Class II/chemistry ; Histocompatibility Antigens Class II/immunology ; Humans ; Protein Conformation
Chemical Substances	Histocompatibility Antigens Class I ; Histocompatibility Antigens Class II
Language	English
Publishing date	2021-08-13
Publishing country	England
Document type	Editorial ; Introductory Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1035767-1
ISSN	1879-0372 ; 0952-7915
ISSN (online)	1879-0372
ISSN	0952-7915
DOI	10.1016/j.coi.2021.07.008
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2773: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 13: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: H2-O deficiency promotes regulatory T cell differentiation and CD4 T cell hyperactivity.

Welsh, Robin A / Song, Nianbin / Park, Chan-Su / Peske, J David / Sadegh-Nasseri, Scheherazade

Frontiers in immunology

2024 Volume 14, Page(s) 1304798

Abstract: Regulatory T cells (Treg) are crucial immune modulators, yet the exact mechanism of thymic Treg development remains controversial. Here, we present the first direct evidence for H2-O, an MHC class II peptide editing molecular chaperon, on selection of ... ...

Abstract	Regulatory T cells (Treg) are crucial immune modulators, yet the exact mechanism of thymic Treg development remains controversial. Here, we present the first direct evidence for H2-O, an MHC class II peptide editing molecular chaperon, on selection of thymic Tregs. We identified that lack of H2-O in the thymic medulla promotes thymic Treg development and leads to an increased peripheral Treg frequency. Single-cell RNA-sequencing (scRNA-seq) analysis of splenic CD4 T cells revealed not only an enrichment of effector-like Tregs, but also activated CD4 T cells in the absence of H2-O. Our data support two concepts; a) lack of H2-O expression in the thymic medulla creates an environment permissive to Treg development and, b) that loss of H2-O drives increased basal auto-stimulation of CD4 T cells. These findings can help in better understanding of predispositions to autoimmunity and design of therapeutics for treatment of autoimmune diseases.
MeSH term(s)	Humans ; CD4-Positive T-Lymphocytes ; Lymphocyte Activation/genetics ; T-Lymphocytes, Regulatory ; Histocompatibility Antigens Class II ; Autoimmune Diseases ; Cell Differentiation
Chemical Substances	Histocompatibility Antigens Class II
Language	English
Publishing date	2024-01-05
Publishing country	Switzerland
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2023.1304798
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Discovering how DO regulates lymphocyte function

Sadegh-Nasseri, Scheherazade / Song, Nianbin / Welsh, Robin

Molecular immunology. 2022 Oct., v. 150

2022

Abstract: HLA-DO or H-2O (DO) in mice is a non-classical, non-polymorphic MHC II molecule primarily expressed in the thymic medulla and B cells. While the common belief is that DO inhibits the activity of DM, controversial data opposes that point of view. Applying ...

Abstract	HLA-DO or H-2O (DO) in mice is a non-classical, non-polymorphic MHC II molecule primarily expressed in the thymic medulla and B cells. While the common belief is that DO inhibits the activity of DM, controversial data opposes that point of view. Applying physicochemical studies to purified DO, DM, and HLA-DR1, we have demonstrated that DO interacts directly with HLA-DR1 molecules in a peptide-receptive-conformation, augmenting binding of ‘DM-resistant’ peptides, while inhibiting binding of ‘DM-sensitive’ peptides to HLA-DR1. Indeed, we have observed that DO-WT B cells express higher densities of pMHC from naturally processed protein antigens in vivo. Accordingly, DO-KO mice are more susceptible to development of EAE due to presence of larger numbers MOG/IAb-specific CD4 T cells in periphery, likely due to a less effective thymic deletion. Since DO is also expressed in B cells, and B cell antigen presentation is a critical step in differentiation of CD4 memory T cells, we tested the contributions of DO to the development of CD4 memory T cells. To do so, we used DR1+/DO-KO mice, and examined CD4 memory development to DR1 restricted flu specific T cells three months post flu vaccine immunization. We found that consistent with our hypothesis, the DR1+/DO-KO mice had fewer flu specific CD4 memory T cells than DR1+/DO-WT mice. By in vitro and in vivo recall experiments, we also found that the secondary immunological responses in DR1+/DO-KO mice were compromised. In addition, we recovered fewer memory B cells from immunized DR1+/DO-KO mice. These results demonstrate that in the absence of DO, the presentation of cognate foreign antigens in the DO-KO mice is altered and can potentially hamper the proper development of memory cells after the resolution of the infection. These findings support the idea that the expression of DO might ensure the proper development of memory cells.
Keywords	B-lymphocytes ; CD4-positive T-lymphocytes ; antigen presentation ; immunization ; influenza ; influenza vaccines ; memory ; peptides
Language	English
Dates of publication	2022-10
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	424427-8
ISSN	1872-9142 ; 0161-5890
ISSN (online)	1872-9142
ISSN	0161-5890
DOI	10.1016/j.molimm.2022.05.109
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 166: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: H2-O deficiency promotes regulatory T cell differentiation and CD4 T cell hyperactivity.

Welsh, Robin A / Song, Nianbin / Park, Chan-Su / Peske, J David / Sadegh-Nasseri, Scheherazade

bioRxiv : the preprint server for biology

2023

Abstract	Regulatory T cells (Treg) are crucial immune modulators, yet the exact mechanism of thymic Treg development remains controversial. Here, we present the first direct evidence for H2-O, an MHC class II peptide editing molecular chaperon, on selection of thymic Tregs. We provide evidence that lack of H2-O in the thymic medulla promotes thymic Treg development and leads to an increased peripheral Treg frequency. Single-cell RNA-sequencing (scRNA-seq) analysis of splenic CD4 T cells revealed not only of an enrichment of effector-like Tregs but also of activated CD4 T cells in the absence of H2-O. Our data support two concepts; a) lack of H2-O expression in the thymic medulla creates an environment permissive to Treg development and, b) that loss of H2-O drives increased basal auto-stimulation of CD4 T cells. These findings can help in better understanding of predispositions to autoimmunity and design of therapeutics for treatment of autoimmune diseases.
Language	English
Publishing date	2023-09-30
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.08.14.553240
Database	MEDical Literature Analysis and Retrieval System OnLINE

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: How Does B Cell Antigen Presentation Affect Memory CD4 T Cell Differentiation and Longevity?

Welsh, Robin A / Song, Nianbin / Sadegh-Nasseri, Scheherazade

Frontiers in immunology

2021 Volume 12, Page(s) 677036

Abstract: Dendritic cells are the antigen presenting cells that process antigens effectively and prime the immune system, a characteristic that have gained them the spotlights in recent years. B cell antigen presentation, although less prominent, deserves equal ... ...

Abstract	Dendritic cells are the antigen presenting cells that process antigens effectively and prime the immune system, a characteristic that have gained them the spotlights in recent years. B cell antigen presentation, although less prominent, deserves equal attention. B cells select antigen experienced CD4 T cells to become memory and initiate an orchestrated genetic program that maintains memory CD4 T cells for life of the individual. Over years of research, we have demonstrated that low levels of antigens captured by B cells during the resolution of an infection render antigen experienced CD4 T cells into a quiescent/resting state. Our studies suggest that in the absence of antigen, the resting state associated with low-energy utilization and proliferation can help memory CD4 T cells to survive nearly throughout the lifetime of mice. In this review we would discuss the primary findings from our lab as well as others that highlight our understanding of B cell antigen presentation and the contributions of the MHC Class II accessory molecules to this outcome. We propose that the quiescence induced by the low levels of antigen presentation might be a mechanism necessary to regulate long-term survival of CD4 memory T cells and to prevent cross-reactivity to autoantigens, hence autoimmunity.
MeSH term(s)	Animals ; Antigen Presentation ; Antigen-Presenting Cells/immunology ; Antigens/immunology ; B-Lymphocytes/immunology ; CD4-Positive T-Lymphocytes/immunology ; Cell Differentiation/immunology ; Histocompatibility Antigens Class II/immunology ; Humans ; Immunologic Memory ; Longevity/immunology ; Lymphocyte Activation/immunology ; Mice
Chemical Substances	Antigens ; Histocompatibility Antigens Class II
Language	English
Publishing date	2021-06-10
Publishing country	Switzerland
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2021.677036
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Commensal bacteria maintain a Qa-1

Guan, Jian / Peske, J David / Manoharan Valerio, Michael / Park, Chansu / Robey, Ellen A / Sadegh-Nasseri, Scheherazade

eLife

2023 Volume 12

Abstract: Intestinal intraepithelial lymphocytes (IELs) are characterized by an unusual phenotype and developmental pathway, yet their specific ligands and functions remain largely unknown. Here by analysis of QFL T cells, a population of ... ...

Abstract	Intestinal intraepithelial lymphocytes (IELs) are characterized by an unusual phenotype and developmental pathway, yet their specific ligands and functions remain largely unknown. Here by analysis of QFL T cells, a population of CD8
MeSH term(s)	Animals ; Mice ; CD8-Positive T-Lymphocytes ; Epithelium ; Bacteria ; Cytokines ; Intestinal Mucosa
Chemical Substances	Cytokines
Language	English
Publishing date	2023-12-21
Publishing country	England
Document type	Journal Article
ZDB-ID	2687154-3
ISSN	2050-084X ; 2050-084X
ISSN (online)	2050-084X
ISSN	2050-084X
DOI	10.7554/eLife.90466
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Commensal Bacteria Maintain a Qa-1

Guan, Jian / Peske, J David / Valerio, Michael Manoharan / Park, Chansu / Robey, Ellen A / Sadegh-Nasseri, Scheherazade

bioRxiv : the preprint server for biology

2023

Abstract	Intestinal intraepithelial lymphocytes (IELs) are characterized by an unusual phenotype and developmental pathway, yet their specific ligands and functions remain largely unknown. Here by analysis of QFL T cells, a population of CD8
Language	English
Publishing date	2023-10-11
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.03.01.530600
Database	MEDical Literature Analysis and Retrieval System OnLINE

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Inter-library loan at ZB MED