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  1. Article ; Online: Assembling the Bacillus subtilis Spore Coat Basement Layer on Spherical Supported Lipid Bilayers.

    Updegrove, Taylor B / D'Atri, Domenico / Ramamurthi, Kumaran S

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2727, Page(s) 215–225

    Abstract: Micro- and nanoparticles are often designed by mimicking naturally occurring structures. Bacterial spores are dormant cells elaborated by some Gram-positive bacteria during poor growth conditions to protect their genetic material from harsh environmental ...

    Abstract Micro- and nanoparticles are often designed by mimicking naturally occurring structures. Bacterial spores are dormant cells elaborated by some Gram-positive bacteria during poor growth conditions to protect their genetic material from harsh environmental stresses. In Bacillus subtilis, this protection is, in part, conferred by a proteinaceous shell, the "coat", which is composed of ~80 different proteins. The basement layer of the coat contains two unusual proteins, which we have recently reconstituted around silica beads to generate synthetic spore-like particles termed "SSHELs". Here, we describe the protocol for generating SSHEL particles, and describe the procedure to covalently link molecules of interest (in this case an anti-HER2 affibody) to SSHEL surfaces. SSHELs therefore represent a versatile platform for the display of ligands or antigens for the site-specific delivery of cargo or vaccines.
    MeSH term(s) Lipid Bilayers/metabolism ; Bacillus subtilis/metabolism ; Bacterial Proteins/metabolism ; Cell Wall/metabolism ; Spores, Bacterial/genetics
    Chemical Substances Lipid Bilayers ; Bacterial Proteins
    Language English
    Publishing date 2023-10-10
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Intramural ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3491-2_17
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: How bacteria block their own biofilms.

    Delerue, Thomas / Ramamurthi, Kumaran S

    The Journal of biological chemistry

    2021  Volume 296, Page(s) 100392

    Abstract: Bacterial biofilms are surface-associated multicellular communities that are highly resistant to removal. Scheffler et al. discovered that Pseudomonas aeruginosa secretes a small molecule that inhibits other P. aeruginosa cells from adsorbing to surfaces ...

    Abstract Bacterial biofilms are surface-associated multicellular communities that are highly resistant to removal. Scheffler et al. discovered that Pseudomonas aeruginosa secretes a small molecule that inhibits other P. aeruginosa cells from adsorbing to surfaces by interfering with type IV pili dynamics. The inhibition of cell adsorption could present a method to prevent biofilm formation on sensitive surfaces in hospitals and industry.
    MeSH term(s) Adsorption ; Bacterial Adhesion ; Biofilms ; Staphylococcus aureus/metabolism ; Staphylococcus aureus/physiology
    Language English
    Publishing date 2021-03-04
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Intramural
    ZDB-ID 2997-x
    ISSN 1083-351X ; 0021-9258
    ISSN (online) 1083-351X
    ISSN 0021-9258
    DOI 10.1016/j.jbc.2021.100392
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  3. Article ; Online: Editorial overview: Growth and development: prokaryotes.

    Ramamurthi, Kumaran S

    Current opinion in microbiology

    2016  Volume 34, Page(s) vii–viii

    MeSH term(s) Cell Proliferation ; Prokaryotic Cells/cytology ; Prokaryotic Cells/physiology
    Language English
    Publishing date 2016-10-05
    Publishing country England
    Document type Editorial ; Introductory Journal Article
    ZDB-ID 1418474-6
    ISSN 1879-0364 ; 1369-5274
    ISSN (online) 1879-0364
    ISSN 1369-5274
    DOI 10.1016/j.mib.2016.09.001
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  4. Article ; Online: Cytoskeletal proteins: lessons learned from bacteria.

    Ramos-León, Félix / Ramamurthi, Kumaran S

    Physical biology

    2022  Volume 19, Issue 2

    Abstract: Cytoskeletal proteins are classified as a group that is defined functionally, whose members are capable of polymerizing into higher order structures, either dynamically or statically, to perform structural roles during a variety of cellular processes. In ...

    Abstract Cytoskeletal proteins are classified as a group that is defined functionally, whose members are capable of polymerizing into higher order structures, either dynamically or statically, to perform structural roles during a variety of cellular processes. In eukaryotes, the most well-studied cytoskeletal proteins are actin, tubulin, and intermediate filaments, and are essential for cell shape and movement, chromosome segregation, and intracellular cargo transport. Prokaryotes often harbor homologs of these proteins, but in bacterial cells, these homologs are usually not employed in roles that can be strictly defined as 'cytoskeletal'. However, several bacteria encode other proteins capable of polymerizing which, although they do not appear to have a eukaryotic counterpart, nonetheless appear to perform a more traditional 'cytoskeletal' function. In this review, we discuss recent reports that cover the structures and functions of prokaryotic proteins that are broadly termed as cytoskeletal, either by sequence homology or by function, to highlight how the enzymatic properties of traditionally studied cytoskeletal proteins may be used for other types of cellular functions; and to demonstrate how truly 'cytoskeletal' functions may be performed by uniquely bacterial proteins that do not display homology to eukaryotic proteins.
    MeSH term(s) Actins/metabolism ; Bacteria/genetics ; Bacterial Proteins/metabolism ; Cytoskeletal Proteins/metabolism ; Cytoskeleton/metabolism
    Chemical Substances Actins ; Bacterial Proteins ; Cytoskeletal Proteins
    Language English
    Publishing date 2022-02-17
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Intramural ; Review
    ZDB-ID 2133216-2
    ISSN 1478-3975 ; 1478-3967
    ISSN (online) 1478-3975
    ISSN 1478-3967
    DOI 10.1088/1478-3975/ac4ef0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Cell division machinery drives cell-specific gene activation during bacterial differentiation.

    Chareyre, Sylvia / Li, Xuesong / Anjuwon-Foster, Brandon R / Clifford, Sarah / Brogan, Anna / Su, Yijun / Shroff, Hari / Ramamurthi, Kumaran S

    bioRxiv : the preprint server for biology

    2023  

    Abstract: When faced with starvation, the ... ...

    Abstract When faced with starvation, the bacterium
    Language English
    Publishing date 2023-08-10
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.08.10.552768
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Bacterial cell surface nanoenvironment requires a specialized chaperone to activate a peptidoglycan biosynthetic enzyme.

    Delerue, Thomas / Chareyre, Sylvia / Anantharaman, Vivek / Gilmore, Michael C / Popham, David L / Cava, Felipe / Aravind, L / Ramamurthi, Kumaran S

    bioRxiv : the preprint server for biology

    2023  

    Abstract: ... Bacillus ... ...

    Abstract Bacillus subtilis
    Language English
    Publishing date 2023-10-06
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.10.06.561273
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Cell division machinery drives cell-specific gene activation during differentiation in

    Chareyre, Sylvia / Li, Xuesong / Anjuwon-Foster, Brandon R / Updegrove, Taylor B / Clifford, Sarah / Brogan, Anna P / Su, Yijun / Zhang, Lixia / Chen, Jiji / Shroff, Hari / Ramamurthi, Kumaran S

    Proceedings of the National Academy of Sciences of the United States of America

    2024  Volume 121, Issue 13, Page(s) e2400584121

    Abstract: When faced with starvation, the ... ...

    Abstract When faced with starvation, the bacterium
    MeSH term(s) Bacillus subtilis/metabolism ; Transcriptional Activation ; Bacterial Proteins/metabolism ; Spores, Bacterial/genetics ; Spores, Bacterial/metabolism ; Cell Division/genetics ; Sigma Factor/genetics ; Sigma Factor/metabolism
    Chemical Substances Bacterial Proteins ; Sigma Factor
    Language English
    Publishing date 2024-03-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2400584121
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  8. Article: Protein coopted from a phage restriction system dictates orthogonal cell division plane selection in

    Ramos-León, Félix / Anjuwon-Foster, Brandon R / Anantharaman, Vivek / Ferreira, Colby N / Ibrahim, Amany M / Tai, Chin-Hsien / Missiakas, Dominique M / Camberg, Jodi L / Aravind, L / Ramamurthi, Kumaran S

    bioRxiv : the preprint server for biology

    2023  

    Abstract: The spherical ... ...

    Abstract The spherical bacterium
    Language English
    Publishing date 2023-09-03
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.09.03.556088
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  9. Article ; Online: Bacterial Cell Division: Nonmodels Poised to Take the Spotlight.

    Eswara, Prahathees J / Ramamurthi, Kumaran S

    Annual review of microbiology

    2017  Volume 71, Page(s) 393–411

    Abstract: The last three decades have witnessed an explosion of discoveries about the mechanistic details of binary fission in model bacteria such as Escherichia coli, Bacillus subtilis, and Caulobacter crescentus. This was made possible not only by advances in ... ...

    Abstract The last three decades have witnessed an explosion of discoveries about the mechanistic details of binary fission in model bacteria such as Escherichia coli, Bacillus subtilis, and Caulobacter crescentus. This was made possible not only by advances in microscopy that helped answer questions about cell biology but also by clever genetic manipulations that directly and easily tested specific hypotheses. More recently, research using understudied organisms, or nonmodel systems, has revealed several alternate mechanistic strategies that bacteria use to divide and propagate. In this review, we highlight new findings and compare these strategies to cell division mechanisms elucidated in model organisms.
    MeSH term(s) Bacteria/growth & development ; Bacteriological Techniques/methods ; Cell Division
    Language English
    Publishing date 2017-07-11
    Publishing country United States
    Document type Journal Article ; Review ; Research Support, N.I.H., Intramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 207931-8
    ISSN 1545-3251 ; 0066-4227
    ISSN (online) 1545-3251
    ISSN 0066-4227
    DOI 10.1146/annurev-micro-102215-095657
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  10. Article ; Online: Cell Death Pathway That Monitors Spore Morphogenesis.

    Decker, Amanda R / Ramamurthi, Kumaran S

    Trends in microbiology

    2017  Volume 25, Issue 8, Page(s) 637–647

    Abstract: The use of quality control mechanisms to stall developmental pathways or completely remove defective cells from a population is a widespread strategy to ensure the integrity of morphogenetic programs. Endospore formation (sporulation) is a well conserved ...

    Abstract The use of quality control mechanisms to stall developmental pathways or completely remove defective cells from a population is a widespread strategy to ensure the integrity of morphogenetic programs. Endospore formation (sporulation) is a well conserved microbial developmental strategy in the Firmicutes phylum wherein a progenitor cell that faces starvation differentiates to form a dormant spore. Despite the conservation of this strategy, it has been unclear what selective pressure maintains the fitness of this developmental program, composed of hundreds of unique genes, during multiple rounds of vegetative growth when sporulation is not required. Recently, a quality control pathway was discovered in Bacillus subtilis which monitors the assembly of the spore envelope and specifically eliminates, through cell lysis, sporulating cells that assemble the envelope incorrectly. Here, we review the use of checkpoints that govern the entry into sporulation in B. subtilis and discuss how the use of regulated cell death pathways during bacterial development may help maintain the fidelity of the sporulation program in the species.
    MeSH term(s) Apoptosis ; Bacillus subtilis/genetics ; Bacillus subtilis/physiology ; Cell Death ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; Lamins/genetics ; Microbial Viability ; Morphogenesis ; Nuclear Envelope/metabolism ; Spores, Bacterial/genetics ; Spores, Bacterial/growth & development ; Spores, Bacterial/physiology
    Chemical Substances Lamins
    Language English
    Publishing date 2017-04-10
    Publishing country England
    Document type Journal Article ; Review ; Research Support, N.I.H., Intramural
    ZDB-ID 1158963-2
    ISSN 1878-4380 ; 0966-842X
    ISSN (online) 1878-4380
    ISSN 0966-842X
    DOI 10.1016/j.tim.2017.03.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 3738: Show issues Location:
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