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Article ; Online: A prevalent exposure to male dog is a risk factor for exclusive allergic sensitization to Can f 5: An Italian multicenter study.

Liccardi, Gennaro / Calzetta, Luigino / Bilò, Maria Beatrice / Brusca, Ignazio / Cecchi, Lorenzo / Costantino, Maria Teresa / Da Re, Mirella / Buzzulini, Francesca / Giani, Mauro / Lombardi, Carlo / Meneguzzi, Giorgia / Meriggi, Antonio / Milanese, Manlio / Onida, Rosa / Patella, Vincenzo / Pignatti, Patrizia / Quercia, Oliviero / Ridolo, Erminia / Sabatino, Giusy /

Sacerdoti, Carlo / Savi, Eleonora / Scala, Enrico / Steinhilber, Gundi / Villalta, Danilo / Passalacqua, Giovanni / Rogliani, Paola

The journal of allergy and clinical immunology. In practice

2020 Volume 8, Issue 7, Page(s) 2399–2401

MeSH term(s)	Allergens ; Animals ; Dogs ; Environmental Exposure ; Humans ; Hypersensitivity/epidemiology ; Italy/epidemiology ; Male ; Risk Factors
Chemical Substances	Allergens
Language	English
Publishing date	2020-03-12
Publishing country	United States
Document type	Journal Article ; Multicenter Study
ZDB-ID	2843237-X
ISSN	2213-2201 ; 2213-2198
ISSN (online)	2213-2201
ISSN	2213-2198
DOI	10.1016/j.jaip.2020.02.041
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Article ; Online: Balance of excitation and inhibition determines 1/f power spectrum in neuronal networks.

Lombardi, F / Herrmann, H J / de Arcangelis, L

Chaos (Woodbury, N.Y.)

2017 Volume 27, Issue 4, Page(s) 47402

Abstract: The 1/f-like decay observed in the power spectrum of electro-physiological signals, along ...

Abstract	The 1/f-like decay observed in the power spectrum of electro-physiological signals, along with scale-free statistics of the so-called neuronal avalanches, constitutes evidence of criticality in neuronal systems. Recent in vitro studies have shown that avalanche dynamics at criticality corresponds to some specific balance of excitation and inhibition, thus suggesting that this is a basic feature of the critical state of neuronal networks. In particular, a lack of inhibition significantly alters the temporal structure of the spontaneous avalanche activity and leads to an anomalous abundance of large avalanches. Here, we study the relationship between network inhibition and the scaling exponent β of the power spectral density (PSD) of avalanche activity in a neuronal network model inspired in Self-Organized Criticality. We find that this scaling exponent depends on the percentage of inhibitory synapses and tends to the value β = 1 for a percentage of about 30%. More specifically, β is close to 2, namely, Brownian noise, for purely excitatory networks and decreases towards values in the interval [1, 1.4] as the percentage of inhibitory synapses ranges between 20% and 30%, in agreement with experimental findings. These results indicate that the level of inhibition affects the frequency spectrum of resting brain activity and suggest the analysis of the PSD scaling behavior as a possible tool to study pathological conditions.
Language	English
Publishing date	2017-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	1472677-4
ISSN	1089-7682 ; 1054-1500
ISSN (online)	1089-7682
ISSN	1054-1500
DOI	10.1063/1.4979043
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Article ; Online: Laser-Induced Fluorescence Emission (L.I.F.E.) as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats.

Weisleitner, Klemens / Hunger, Lars / Kohstall, Christoph / Frisch, Albert / Storrie-Lombardi, Michael C / Sattler, Birgit

Journal of visualized experiments : JoVE

2019 , Issue 152

Abstract: ... Fluorescence Emission (L.I.F.E.) technique. Photoautotrophic supraglacial communities can be identified ... by the detection of L.I.F.E. signatures in photopigments. The L.I.F.E. instrument calibration for the porphyrin ... is demonstrated. For the validation of this methodology, L.I.F.E. data were ratified ...

Abstract	Global warming affects microbial communities in a variety of ecosystems, especially cryospheric habitats. However, little is known about microbial-mediated carbon fluxes in extreme environments. Hence, the methodology of sample acquisition described in the very few studies available implies two major problems: A) high resolution data require a large number of samples, which is difficult to obtain in remote areas; B) unavoidable sample manipulation such as cutting, sawing, and melting of ice cores that leads to a misunderstanding of in situ conditions. In this study, a prototype device that requires neither sample preparation nor sample destruction is presented. The device can be used for in situ measurements with a high spectral and spatial resolution in terrestrial and ice ecosystems and is based on the Laser-Induced Fluorescence Emission (L.I.F.E.) technique. Photoautotrophic supraglacial communities can be identified by the detection of L.I.F.E. signatures in photopigments. The L.I.F.E. instrument calibration for the porphyrin derivates chlorophylla (chla) (405 nm laser excitation) and B-phycoerythrin (B-PE) (532 nm laser excitation) is demonstrated. For the validation of this methodology, L.I.F.E. data were ratified by a conventional method for chla quantification that involved pigment extraction and subsequent absorption spectroscopy. The prototype applicability in the field was proven in extreme polar environments. Further testing on terrestrial habitats took place during Mars analog simulations in the Moroccan dessert and on an Austrian rock glacier. The L.I.F.E. instrument enables high resolution scans of large areas with acceptable operation logistics and contributes to a better understanding of the ecological potential of supraglacial communities in the context of global change.
MeSH term(s)	Biomarkers/metabolism ; Fluorescence ; Global Warming ; Ice Cover/chemistry ; Lasers/standards
Chemical Substances	Biomarkers
Language	English
Publishing date	2019-10-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Video-Audio Media
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/60447
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Article ; Online: Diagnostic role of (18)F-FDG-PET or PET/CT in salivary gland tumors: A systematic review.

Bertagna, F / Nicolai, P / Maroldi, R / Mattavelli, D / Bertoli, M / Giubbini, R / Lombardi, D / Treglia, G

Revista espanola de medicina nuclear e imagen molecular

2015 Volume 34, Issue 5, Page(s) 295–302

Abstract: Salivary gland tumors are rare neoplasms that have not been extensively studied with (18)F-FDG-PET ...

Abstract	Salivary gland tumors are rare neoplasms that have not been extensively studied with (18)F-FDG-PET- or PET/CT up to now. This review aims to evaluate the diagnostic performance of PET in this particular setting, analyzing the available literature. A comprehensive literature search in the PubMed/MEDLINE, Embase and Scopus databases was performed including articles up to November 2014, resulting in the selection of 22 articles. The studies selected suggest that: (1) PET is not useful in discriminating benign from malignant SGTs because of the overlap of uptake in both conditions; (2) PET not only is complementary to conventional imaging techniques for the staging and restaging but in some cases could also be superior to them; (3) PET may often have a highly positive impact on clinical decision making. Despite many limitations affecting the analysis, PET seems to be useful in SGTs. However, more extensive studies and cost-effectiveness analyses are desirable to determine its correct position in the diagnostic flow chart.
MeSH term(s)	Diagnosis, Differential ; Fluorine Radioisotopes ; Fluorodeoxyglucose F18 ; Humans ; Neoplasm Staging/methods ; Positron Emission Tomography Computed Tomography ; Positron-Emission Tomography ; Prognosis ; Radiopharmaceuticals ; Salivary Gland Diseases/diagnosis ; Salivary Gland Neoplasms/diagnosis ; Salivary Gland Neoplasms/diagnostic imaging ; Salivary Gland Neoplasms/pathology
Chemical Substances	Fluorine Radioisotopes ; Radiopharmaceuticals ; Fluorodeoxyglucose F18 (0Z5B2CJX4D)
Language	English
Publishing date	2015-09
Publishing country	Spain
Document type	Journal Article ; Review
ZDB-ID	2911865-7
ISSN	2253-8070 ; 2253-654X
ISSN (online)	2253-8070
ISSN	2253-654X
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Article: Staging of Primary Abdominal Lymphomas: Comparison of Whole-Body MRI with Diffusion-Weighted Imaging and (18)F-FDG-PET/CT.

Stecco, Alessandro / Buemi, Francesco / Quagliozzi, Martina / Lombardi, Mariangela / Santagostino, Alberto / Sacchetti, Gian Mauro / Carriero, Alessandro

Gastroenterology research and practice

2015 Volume 2015, Page(s) 104794

Abstract: ... weighted sequences (WB-DW-MRI) with that of (18)F-FDG-PET/CT in the staging of patients with primary ... gastrointestinal lymphoma. All patients underwent (18)F-FDG-PET/CT and WB-DW-MRI. Histopathology findings or ... Conclusions. WB-DW-MRI appears to have a comparable diagnostic value to (18)F-FDG-PET/CT in staging patients ...

Abstract	Background. The purpose of this study was to compare the accuracy of whole-body MRI with diffusion-weighted sequences (WB-DW-MRI) with that of (18)F-FDG-PET/CT in the staging of patients with primary gastrointestinal lymphoma. Methods. This retrospective study involved 17 untreated patients with primary abdominal gastrointestinal lymphoma. All patients underwent (18)F-FDG-PET/CT and WB-DW-MRI. Histopathology findings or at least 6 months of clinical and radiological follow-up was the gold standard. The Musshoff-modified Ann Arbor system was used for staging, and diagnostic accuracy was evaluated on a per-node basis. Results. WB-DW-MRI exhibited 100% sensitivity, 96.3% specificity, and 96.1% and 100% positive and negative predictive values (PPV and NPV), respectively. The sensitivity, specificity, and PPV and NPV of PET/CT were 95.9%, 100%, and 100% and 96.4%, respectively. There were no statistically significant differences between the two techniques (p = 0.05). The weighted kappa agreement statistics with a 95% confidence interval were 0.97 (0.95-0.99) between the two MRI readers and 0.87 (0.82-0.92) between the two methods. Conclusions. WB-DW-MRI appears to have a comparable diagnostic value to (18)F-FDG-PET/CT in staging patients with gastrointestinal lymphoma.
Language	English
Publishing date	2015-12-22
Publishing country	Egypt
Document type	Journal Article
ZDB-ID	2435460-0
ISSN	1687-630X ; 1687-6121
ISSN (online)	1687-630X
ISSN	1687-6121
DOI	10.1155/2015/104794
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Article: Laser-induced fluorescence emission (l.i.f.e.) as novel non-invasive tool for in-situ measurements of biomarkers in cryospheric habitats

Weisleitner, Klemens / Frisch, Albert / Hunger, Lars / Kohstall, Christoph / Sattler, Birgit / Storrie-Lombardi, Michael C

Journal of visualized experiments. 2019 Oct. 26, , no. 152

2019

Abstract	Global warming affects microbial communities in a variety of ecosystems, especially cryospheric habitats. However, little is known about microbial-mediated carbon fluxes in extreme environments. Hence, the methodology of sample acquisition described in the very few studies available implies two major problems: A) high resolution data require a large number of samples, which is difficult to obtain in remote areas; B) unavoidable sample manipulation such as cutting, sawing, and melting of ice cores that leads to a misunderstanding of in situ conditions. In this study, a prototype device that requires neither sample preparation nor sample destruction is presented. The device can be used for in situ measurements with a high spectral and spatial resolution in terrestrial and ice ecosystems and is based on the Laser-Induced Fluorescence Emission (L.I.F.E.) technique. Photoautotrophic supraglacial communities can be identified by the detection of L.I.F.E. signatures in photopigments. The L.I.F.E. instrument calibration for the porphyrin derivates chlorophylla (chla) (405 nm laser excitation) and B-phycoerythrin (B-PE) (532 nm laser excitation) is demonstrated. For the validation of this methodology, L.I.F.E. data were ratified by a conventional method for chla quantification that involved pigment extraction and subsequent absorption spectroscopy. The prototype applicability in the field was proven in extreme polar environments. Further testing on terrestrial habitats took place during Mars analog simulations in the Moroccan dessert and on an Austrian rock glacier. The L.I.F.E. instrument enables high resolution scans of large areas with acceptable operation logistics and contributes to a better understanding of the ecological potential of supraglacial communities in the context of global change.
Keywords	biomarkers ; carbon ; computer simulation ; desserts ; ecosystems ; fluorescence ; glaciers ; global warming ; habitats ; ice ; melting ; microbial communities ; porphyrins ; prototypes ; sawing ; spectroscopy
Language	English
Dates of publication	2019-1026
Size	p. e60447.
Publishing place	Journal of Visualized Experiments
Document type	Article
ZDB-ID	2259946-0
ISSN	1940-087X
ISSN	1940-087X
DOI	10.3791/60447
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Article ; Online: Field trial of a dual-wavelength fluorescent emission (L.I.F.E.) instrument and the Magma White rover during the MARS2013 Mars analog mission.

Groemer, Gernot / Sattler, Birgit / Weisleitner, Klemens / Hunger, Lars / Kohstall, Christoph / Frisch, Albert / Józefowicz, Mateusz / Meszyński, Sebastian / Storrie-Lombardi, Michael / Bothe, Claudia / Boyd, Andrea / Dinkelaker, Aline / Dissertori, Markus / Fasching, David / Fischer, Monika / Föger, Daniel / Foresta, Luca / Frischauf, Norbert / Fritsch, Lukas /

Fuchs, Harald / Gautsch, Christoph / Gerard, Stephan / Goetzloff, Linda / Gołebiowska, Izabella / Gorur, Paavan / Groemer, Gerhard / Groll, Petra / Haider, Christian / Haider, Olivia / Hauth, Eva / Hauth, Stefan / Hettrich, Sebastian / Jais, Wolfgang / Jones, Natalie / Taj-Eddine, Kamal / Karl, Alexander / Kauerhoff, Tilo / Khan, Muhammad Shadab / Kjeldsen, Andreas / Klauck, Jan / Losiak, Anna / Luger, Markus / Luger, Thomas / Luger, Ulrich / McArthur, Jane / Moser, Linda / Neuner, Julia / Orgel, Csilla / Ori, Gian Gabriele / Paternesi, Roberta / Peschier, Jarno / Pfeil, Isabella / Prock, Silvia / Radinger, Josef / Ragonig, Christoph / Ramirez, Barbara / Ramo, Wissam / Rampey, Mike / Sams, Arnold / Sams, Elisabeth / Sams, Sebastian / Sandu, Oana / Sans, Alejandra / Sansone, Petra / Scheer, Daniela / Schildhammer, Daniel / Scornet, Quentin / Sejkora, Nina / Soucek, Alexander / Stadler, Andrea / Stummer, Florian / Stumptner, Willibald / Taraba, Michael / Tlustos, Reinhard / Toferer, Ernst / Turetschek, Thomas / Winter, Egon / Zanella-Kux, Katja

Astrobiology

2014 Volume 14, Issue 5, Page(s) 391–405

Abstract: ... to produce a laser-induced fluorescence emission (L.I.F.E.) spectral database of the mineral and organic ... environment. We report on the L.I.F.E. instrument setup, data processing, and performance during field trials ...

Abstract	Abstract We have developed a portable dual-wavelength laser fluorescence spectrometer as part of a multi-instrument optical probe to characterize mineral, organic, and microbial species in extreme environments. Operating at 405 and 532 nm, the instrument was originally designed for use by human explorers to produce a laser-induced fluorescence emission (L.I.F.E.) spectral database of the mineral and organic molecules found in the microbial communities of Earth's cryosphere. Recently, our team had the opportunity to explore the strengths and limitations of the instrument when it was deployed on a remote-controlled Mars analog rover. In February 2013, the instrument was deployed on board the Magma White rover platform during the MARS2013 Mars analog field mission in the Kess Kess formation near Erfoud, Morocco. During these tests, we followed tele-science work flows pertinent to Mars surface missions in a simulated spaceflight environment. We report on the L.I.F.E. instrument setup, data processing, and performance during field trials. A pilot postmission laboratory analysis determined that rock samples acquired during the field mission exhibited a fluorescence signal from the Sun-exposed side characteristic of chlorophyll a following excitation at 405 nm. A weak fluorescence response to excitation at 532 nm may have originated from another microbial photosynthetic pigment, phycoerythrin, but final assignment awaits development of a comprehensive database of mineral and organic fluorescence spectra. No chlorophyll fluorescence signal was detected from the shaded underside of the samples.
MeSH term(s)	Mars ; Robotics/instrumentation ; Space Simulation ; Spectrometry, Fluorescence/instrumentation
Language	English
Publishing date	2014-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2047736-3
ISSN	1557-8070 ; 1531-1074
ISSN (online)	1557-8070
ISSN	1531-1074
DOI	10.1089/ast.2013.1081
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Article ; Online: A rare case of solitary fibrous tumor of the adrenal gland detected by (18)F-FDG PET/CT.

Treglia, Giorgio / Oragano, Luigi / Fadda, Guido / Raffaelli, Marco / Lombardi, Celestino Pio / Castaldi, Paola / Rufini, Vittoria

Clinical nuclear medicine

2014 Volume 39, Issue 5, Page(s) 475–477

Abstract: A 33-year old man underwent an F-FDG PET/CT searching for the cause of a fever of unknown origin. F ... that benign solitary fibrous tumor should be considered as possible false-positive F-FDG PET/CT finding ...

Abstract	A 33-year old man underwent an F-FDG PET/CT searching for the cause of a fever of unknown origin. F-FDG PET/CT incidentally detected a focal area of markedly increased radiopharmaceutical uptake corresponding to a 2.5-cm nodule in the right adrenal gland. Laboratory data ruled out the presence of a functioning adrenal lesion. All these findings were suggestive of adrenal malignancy. After right adrenalectomy, histology showed a benign solitary fibrous tumor of the adrenal gland. This case highlights that benign solitary fibrous tumor should be considered as possible false-positive F-FDG PET/CT finding for malignancy in evaluating adrenal incidentalomas.
MeSH term(s)	Adrenal Gland Neoplasms/diagnostic imaging ; Adult ; Fever of Unknown Origin/complications ; Fluorodeoxyglucose F18 ; Humans ; Male ; Multimodal Imaging ; Positron-Emission Tomography ; Solitary Fibrous Tumors/diagnostic imaging ; Tomography, X-Ray Computed
Chemical Substances	Fluorodeoxyglucose F18 (0Z5B2CJX4D)
Language	English
Publishing date	2014-05
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	197628-x
ISSN	1536-0229 ; 0363-9762
ISSN (online)	1536-0229
ISSN	0363-9762
DOI	10.1097/RLU.0b013e31828e9752
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Article ; Online: The lower the better: It is not always the case!

Lombardi, Federico

Heart rhythm

2024

Language	English
Publishing date	2024-01-26
Publishing country	United States
Document type	Editorial
ZDB-ID	2229357-7
ISSN	1556-3871 ; 1547-5271
ISSN (online)	1556-3871
ISSN	1547-5271
DOI	10.1016/j.hrthm.2024.01.044
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Article ; Online: The Arabidopsis COP9 SIGNALOSOME INTERACTING F-BOX KELCH 1 protein forms an SCF ubiquitin ligase and regulates hypocotyl elongation.

Franciosini, Anna / Lombardi, Benedetta / Iafrate, Silvia / Pecce, Valeria / Mele, Giovanni / Lupacchini, Leonardo / Rinaldi, Gianmarco / Kondou, Youichi / Gusmaroli, Giuliana / Aki, Shiori / Tsuge, Tomohiko / Deng, Xing-Wang / Matsui, Minami / Vittorioso, Paola / Costantino, Paolo / Serino, Giovanna

Molecular plant

2013 Volume 6, Issue 5, Page(s) 1616–1629

Abstract: ... plant-specific CSN-interacting F-box protein, which we denominated CFK1 (COP9 INTERACTING F-BOX KELCH 1). We show ...

Abstract	The regulation of protein turnover by the ubiquitin proteasome system (UPS) is a major posttranslational mechanism in eukaryotes. One of the key components of the UPS, the COP9 signalosome (CSN), regulates 'cullin-ring' E3 ubiquitin ligases. In plants, CSN participates in diverse cellular and developmental processes, ranging from light signaling to cell cycle control. In this work, we isolated a new plant-specific CSN-interacting F-box protein, which we denominated CFK1 (COP9 INTERACTING F-BOX KELCH 1). We show that, in Arabidopsis thaliana, CFK1 is a component of a functional ubiquitin ligase complex. We also show that CFK1 stability is regulated by CSN and by proteasome-dependent proteolysis, and that light induces accumulation of the CFK1 transcript in the hypocotyl. Analysis of CFK1 knockdown, mutant, and overexpressing seedlings indicates that CFK1 promotes hypocotyl elongation by increasing cell size. Reduction of CSN levels enhances the short hypocotyl phenotype of CFK1-depleted seedlings, while complete loss of CSN activity suppresses the long-hypocotyl phenotype of CFK1-overexpressing seedlings. We propose that CFK1 (and its regulation by CSN) is a novel component of the cellular mechanisms controlling hypocotyl elongation.
MeSH term(s)	Amino Acid Sequence ; Arabidopsis/cytology ; Arabidopsis/enzymology ; Arabidopsis/growth & development ; Arabidopsis/radiation effects ; Arabidopsis Proteins/chemistry ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; COP9 Signalosome Complex ; Cell Size/radiation effects ; Down-Regulation/radiation effects ; F-Box Proteins/chemistry ; F-Box Proteins/genetics ; F-Box Proteins/metabolism ; Gene Expression Regulation, Plant/radiation effects ; Genes, Plant/genetics ; Hypocotyl/genetics ; Hypocotyl/growth & development ; Hypocotyl/radiation effects ; Light ; Molecular Sequence Data ; Multiprotein Complexes/metabolism ; Mutation/genetics ; Peptide Hydrolases/metabolism ; Phenotype ; Plants, Genetically Modified ; Proteasome Endopeptidase Complex/metabolism ; Protein Stability/radiation effects ; Proteolysis/radiation effects ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; SKP Cullin F-Box Protein Ligases/chemistry ; SKP Cullin F-Box Protein Ligases/genetics ; SKP Cullin F-Box Protein Ligases/metabolism ; Ubiquitination/radiation effects
Chemical Substances	Arabidopsis Proteins ; CFK1 protein, Arabidopsis ; F-Box Proteins ; Multiprotein Complexes ; RNA, Messenger ; SKP Cullin F-Box Protein Ligases (EC 2.3.2.27) ; Peptide Hydrolases (EC 3.4.-) ; COP9 Signalosome Complex (EC 3.4.19.12) ; Proteasome Endopeptidase Complex (EC 3.4.25.1)
Language	English
Publishing date	2013-09
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2393618-6
ISSN	1752-9867 ; 1674-2052
ISSN (online)	1752-9867
ISSN	1674-2052
DOI	10.1093/mp/sst045
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