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Article ; Online: Correction: Establishment and application of a quadruple real-time RT-PCR for detecting avian metapneumovirus.

Wang, Suchun / Jiang, Nan / Jiang, Lijian / Zhuang, Qingye / Chen, Qiong / Hou, Guangyu / Xiao, Zhiyu / Zhao, Ran / Li, Yang / Zhao, Chenglong / Zhang, Fuyou / Yu, Jianmin / Li, Jinping / Liu, Hualei / Sun, Fuliang / Wang, Kaicheng

PloS one

2023 Volume 18, Issue 11, Page(s) e0294298

Abstract: This corrects the article DOI: 10.1371/journal.pone.0270708.]. ...

Abstract	[This corrects the article DOI: 10.1371/journal.pone.0270708.].
Language	English
Publishing date	2023-11-07
Publishing country	United States
Document type	Published Erratum
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0294298
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Correction

Suchun Wang / Nan Jiang / Lijian Jiang / Qingye Zhuang / Qiong Chen / Guangyu Hou / Zhiyu Xiao / Ran Zhao / Yang Li / Chenglong Zhao / Fuyou Zhang / Jianmin Yu / Jinping Li / Hualei Liu / Fuliang Sun / Kaicheng Wang

PLoS ONE, Vol 18, Iss 11, p e

Establishment and application of a quadruple real-time RT-PCR for detecting avian metapneumovirus.

2023 Volume 0294298

Abstract: This corrects the article DOI:10.1371/journal.pone.0270708.]. ...

Abstract	[This corrects the article DOI:10.1371/journal.pone.0270708.].
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Molecular epidemiology analysis of fowl adenovirus detected from apparently healthy birds in eastern China

Zhuang, Qingye / Wang, Suchun / Zhang, Fuyou / Zhao, Chenglong / Chen, Qiong / Zhao, Ran / Guo, Pin / Ju, Lei / Li, Jinping / Hou, Guangyu / Chen, Xiaoying / Sun, Fuliang / Wang, Kaicheng

BMC Vet Res. 2023 Dec., v. 19, no. 1 p.5-5

2023

Abstract: BACKGROUND: Fowl adenovirus is of major concern to the poultry industry worldwidely. In order to monitor the prevalent status of Fowl adenovirus in China, a total of 1920 clinical samples from apparently healthy birds in the 25 sites of poultry flocks, ... ...

Abstract	BACKGROUND: Fowl adenovirus is of major concern to the poultry industry worldwidely. In order to monitor the prevalent status of Fowl adenovirus in China, a total of 1920 clinical samples from apparently healthy birds in the 25 sites of poultry flocks, Slaughterhouse and living bird markets from 8 provinces in eastern China were collected and detected by PCR, sequencing, and phylogenetic analysis. RESULTS: The epidemiological survey showed that Fowl adenoviruses were detected in living bird markets, and circulating in a variety of fowl species, including chickens, ducks, goose and pigeons. Among the 1920 clinical samples, 166 samples (8.65%) were positive in the fowl adenovirus PCR detection. In this study, totally all the 12 serotypes (serotypes of 1, 2, 3, 4, 5, 6, 7, 8A, 8B, 9, 10 and 11) fowl adenoviruses were detected, the most prevalent serotype was serotype 1. Phylogenetic analysis indicated that 166 FAdVs of 12 serotypes were divided into 5 fowl adenovirus species (Fowl aviadenovirus A, B, C, D, E). CONCLUSIONS: In the epidemiological survey, 8.65% of the clinical samples from apparently healthy birds were positive in the fowl adenovirus PCR detection. Totally all the 12 serotypes fowl adenoviruses were detected in a variety of fowl species, which provided abundant resources for the research of fowl adenoviruses in China. The newly prevalent FAdV serotypes provides valuable information for the development of an effective control strategy for FAdV infections in fowls.
Keywords	Fowl aviadenovirus A ; epidemiological studies ; geese ; molecular epidemiology ; phylogeny ; poultry industry ; serotypes ; slaughterhouses ; China
Language	English
Dates of publication	2023-12
Size	p. 5.
Publishing place	BioMed Central
Document type	Article ; Online
ZDB-ID	2191675-5
ISSN	1746-6148
ISSN	1746-6148
DOI	10.1186/s12917-022-03545-5
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Reverse transcription recombinase-aided amplification assay for avian influenza virus.

Wang, Suchun / Zhuang, Qingye / Jiang, Nan / Zhang, Fuyou / Chen, Qiong / Zhao, Ran / Li, Yang / Yu, Xiaohui / Li, Jinping / Hou, Guangyu / Yuan, Liping / Sun, Fuliang / Pan, Zihao / Wang, Kaicheng

Virus genes

2023 Volume 59, Issue 3, Page(s) 410–416

Abstract: Avian influenza virus (AIV) infection can lead to severe economic losses in the poultry industry and causes a serious risk for humans. A rapid and simple test for suspected viral infection cases is crucial. In this study, a reverse transcription ... ...

Abstract	Avian influenza virus (AIV) infection can lead to severe economic losses in the poultry industry and causes a serious risk for humans. A rapid and simple test for suspected viral infection cases is crucial. In this study, a reverse transcription recombinase-aided amplification assay (RT-RAA) for the rapid detection of all AIV subtypes was developed. The reaction temperature of the assays is at 39 °C and the detection process can be completed in less than 20 min. The specificity results of the assay showed that this method had no cross-reaction with other main respiratory viruses that affect birds, including Newcastle disease virus (NDV) and infectious bronchitis virus (IBV). The analytical sensitivity at a 95% confidence interval was 10
MeSH term(s)	Animals ; Humans ; Reverse Transcription ; Influenza in Birds/diagnosis ; Recombinases/genetics ; Recombinases/metabolism ; Influenza A virus/genetics ; Birds/genetics ; Sensitivity and Specificity
Chemical Substances	Recombinases
Language	English
Publishing date	2023-02-13
Publishing country	United States
Document type	Journal Article
ZDB-ID	639496-6
ISSN	1572-994X ; 0920-8569
ISSN (online)	1572-994X
ISSN	0920-8569
DOI	10.1007/s11262-023-01979-z
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Article ; Online: Surveillance and genetic diversity analysis of avian astrovirus in China.

Zhang, Fuyou / Li, Yang / Jiang, Wenming / Yu, Xiaohui / Zhuang, Qingye / Wang, Suchun / Yuan, Liping / Wang, Kaicheng / Sun, Shuhong / Liu, Hualei

PloS one

2022 Volume 17, Issue 2, Page(s) e0264308

Abstract: Avian astroviruses (AAstVs) have caused major problem for poultry breeding industries in China in recent years, and the goose gout caused by goose astrovirus has produced particularly great economic losses. To better understand the prevalence and genetic ...

Abstract	Avian astroviruses (AAstVs) have caused major problem for poultry breeding industries in China in recent years, and the goose gout caused by goose astrovirus has produced particularly great economic losses. To better understand the prevalence and genetic diversity of AAstVs in China, 1210 poultry samples collected from eight provinces were tested with reverse transcription-polymerase chain reaction (RT-PCR) to detect AAstV infections in different poultry populations. Then, Open reading frames 2 (ORF2) was amplified by specific primers, and the genetic evolution was analyzed. Our surveillance data demonstrate the diversity of AAstVs in China insofar as we detected 17 AAstVs, including seven chicken astroviruses (CAstVs), five avian nephritis viruses (ANVs), two goose astroviruses (GoAstVs), two duck astrovirus (DAstVs), and one new AAstV belonging to Avastrovirus Group 3. The positive rate of AAstV infection was 1.40%. Host analysis showed that CAstVs and ANVs were isolated from chickens, DAstVs and GoAstVs were isolated from ducks. Host-species-specific AAstVs infections were also identified in numerous samples collected at each stage of production. This study provides further evidence to better understand the epidemiology of AAstVs in different species of poultry in China.
MeSH term(s)	Animals ; Astroviridae Infections/genetics ; Avastrovirus/genetics ; Chickens/virology ; Ducks/virology ; Geese/virology ; Genetic Variation ; Genome, Viral ; Phylogeny ; Poultry Diseases/genetics ; Poultry Diseases/virology
Language	English
Publishing date	2022-02-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0264308
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Research on the collaborative governance of urban regeneration based on a Bayesian network: The case of Chongqing

Liu, Guiwen / Fu, Xinyue / Han, Qingye / Huang, Ruopeng / Zhuang, Taozhi

Land use policy. 2021 Oct., v. 109

2021

Abstract: The rapid development of the economy and urbanization has led to numerous urban regeneration projects, and collaborative governance is increasingly advocated to address the management issues that occur during urban regeneration. However, strong ... ...

Abstract	The rapid development of the economy and urbanization has led to numerous urban regeneration projects, and collaborative governance is increasingly advocated to address the management issues that occur during urban regeneration. However, strong collaboration is still a great challenge among multiple stakeholders during the process of urban regeneration. Based on Bayesian network theory and the case of Chongqing, this article aims to deconstruct the complex structure and explore the inherent mechanism of urban regeneration governance to achieve collaboration. First, a Bayesian network model of urban regeneration governance was developed to visualize the complex structure of multiple stakeholders’ interests. Second, the inherent mechanism was analyzed though Bayesian inference. The results indicate that the cooperation degree of related governments, conflict resolution efficiency, degree of public participation, and normality of public participation may be key factors that lead to collaborative behavior. Third, a further discussion was conducted, based on which policy implications were drawn to promote collaborative governance in urban regeneration.
Keywords	Bayesian theory ; citizen participation ; conflict management ; governance ; land policy ; models ; network theory ; stakeholders ; urbanization
Language	English
Dates of publication	2021-10
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	852476-2
ISSN	0264-8377
ISSN	0264-8377
DOI	10.1016/j.landusepol.2021.105640
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Surveillance and genetic diversity analysis of avian astrovirus in China

Fuyou Zhang / Yang Li / Wenming Jiang / Xiaohui Yu / Qingye Zhuang / Suchun Wang / Liping Yuan / Kaicheng Wang / Shuhong Sun / Hualei Liu

PLoS ONE, Vol 17, Iss

2022 Volume 2

Abstract	Avian astroviruses (AAstVs) have caused major problem for poultry breeding industries in China in recent years, and the goose gout caused by goose astrovirus has produced particularly great economic losses. To better understand the prevalence and genetic diversity of AAstVs in China, 1210 poultry samples collected from eight provinces were tested with reverse transcription-polymerase chain reaction (RT-PCR) to detect AAstV infections in different poultry populations. Then, Open reading frames 2 (ORF2) was amplified by specific primers, and the genetic evolution was analyzed. Our surveillance data demonstrate the diversity of AAstVs in China insofar as we detected 17 AAstVs, including seven chicken astroviruses (CAstVs), five avian nephritis viruses (ANVs), two goose astroviruses (GoAstVs), two duck astrovirus (DAstVs), and one new AAstV belonging to Avastrovirus Group 3. The positive rate of AAstV infection was 1.40%. Host analysis showed that CAstVs and ANVs were isolated from chickens, DAstVs and GoAstVs were isolated from ducks. Host-species-specific AAstVs infections were also identified in numerous samples collected at each stage of production. This study provides further evidence to better understand the epidemiology of AAstVs in different species of poultry in China.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Reverse transcription recombinase-aided amplification assay for H5 subtype avian influenza virus.

Wang, Suchun / Li, Yang / Zhang, Fuyou / Jiang, Nan / Zhuang, Qingye / Hou, Guangyu / Jiang, Lijian / Yu, Jianmin / Yu, Xiaohui / Liu, Hualei / Zhao, Chenglong / Yuan, Liping / Huang, Baoxu / Wang, Kaicheng

Virology journal

2022 Volume 19, Issue 1, Page(s) 129

Abstract: Background: The H5 subtype avian influenza virus (AIV) has caused huge economic losses to the poultry industry and is a threat to human health. A rapid and simple test is needed to confirm infection in suspected cases during disease outbreaks.: ... ...

Abstract	Background: The H5 subtype avian influenza virus (AIV) has caused huge economic losses to the poultry industry and is a threat to human health. A rapid and simple test is needed to confirm infection in suspected cases during disease outbreaks. Methods: In this study, we developed a reverse transcription recombinase-aided amplification (RT-RAA) assay for the detection of H5 subtype AIV. Assays were performed at a single temperature (39 °C), and the results were obtained within 20 min. Results: The assay showed no cross-detection with Newcastle disease virus or infectious bronchitis virus. The analytical sensitivity was 10 Conclusions: These results indicated that our RT-RAA assay may be a valuable tool for detecting H5 subtype AIV.
MeSH term(s)	Animals ; Birds ; Humans ; Influenza A virus/genetics ; Influenza A virus/metabolism ; Influenza in Birds/diagnosis ; Recombinases/genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Reverse Transcription ; Sensitivity and Specificity
Chemical Substances	Recombinases
Language	English
Publishing date	2022-07-30
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2160640-7
ISSN	1743-422X ; 1743-422X
ISSN (online)	1743-422X
ISSN	1743-422X
DOI	10.1186/s12985-022-01807-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Establishment and application of a quadruple real-time RT-PCR for detecting avian metapneumovirus.

PloS one

2022 Volume 17, Issue 6, Page(s) e0270708

Abstract: In order to develop an appropriate method for high-throughput detection of avian metapneumovirus, a quadruple real-time reverse-transcription polymerase chain reaction assay was established with four pairs of specific primers and four specific probes ... ...

Abstract	In order to develop an appropriate method for high-throughput detection of avian metapneumovirus, a quadruple real-time reverse-transcription polymerase chain reaction assay was established with four pairs of specific primers and four specific probes based on the G or M gene of aMPV-A, aMPV-B, aMPV-C and aMPV-D. Its specificity and sensitivity were evaluated, and clinical samples were tested by the method. The results showed that all the four subgroups of avian metapneumovirus can be detected in the quadruple real-time RT-PCR assay simultaneously, with a detection limit of 100-1000 cRNA copies/reaction. The other common poultry viruses were negative. In the avian clinical sample detection, 39 out of 1920 clinical samples collected from 8 provinces were positive. Compared with published RT-PCR assays, the κ value of the quadruple real-time RT-PCR assay in 1920 avian clinical samples was 1.000 (P < 0.001). The established method could be used for the rapid detection of the four subgroups of avian metapneumovirus with high specificity and high sensitivity.
MeSH term(s)	Animals ; Birds/genetics ; Metapneumovirus/genetics ; Poultry Diseases/diagnosis ; Real-Time Polymerase Chain Reaction/methods ; Reverse Transcriptase Polymerase Chain Reaction ; Sensitivity and Specificity
Language	English
Publishing date	2022-06-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0270708
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Next-generation sequencing library preparation method for identification of RNA viruses on the Ion Torrent Sequencing Platform.

Chen, Guiqian / Qiu, Yuan / Zhuang, Qingye / Wang, Suchun / Wang, Tong / Chen, Jiming / Wang, Kaicheng

Virus genes

2018 Volume 54, Issue 4, Page(s) 536–542

Abstract: Next generation sequencing (NGS) is a powerful tool for the characterization, discovery, and molecular identification of RNA viruses. There were multiple NGS library preparation methods published for strand-specific RNA-seq, but some methods are not ... ...

Abstract	Next generation sequencing (NGS) is a powerful tool for the characterization, discovery, and molecular identification of RNA viruses. There were multiple NGS library preparation methods published for strand-specific RNA-seq, but some methods are not suitable for identifying and characterizing RNA viruses. In this study, we report a NGS library preparation method to identify RNA viruses using the Ion Torrent PGM platform. The NGS sequencing adapters were directly inserted into the sequencing library through reverse transcription and polymerase chain reaction, without fragmentation and ligation of nucleic acids. The results show that this method is simple to perform, able to identify multiple species of RNA viruses in clinical samples.
MeSH term(s)	Animals ; China ; Cloaca/virology ; Feces/virology ; Gene Library ; High-Throughput Nucleotide Sequencing/methods ; Poultry ; RNA Viruses/classification ; RNA Viruses/genetics ; RNA Viruses/isolation & purification ; RNA, Viral/genetics ; Reverse Transcriptase Polymerase Chain Reaction/methods ; Trachea/virology
Chemical Substances	RNA, Viral
Keywords	covid19
Language	English
Publishing date	2018-05-09
Publishing country	United States
Document type	Journal Article
ZDB-ID	639496-6
ISSN	1572-994X ; 0920-8569
ISSN (online)	1572-994X
ISSN	0920-8569
DOI	10.1007/s11262-018-1568-x
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

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