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  1. Article: Influence of different sample preparation techniques on imaging viruses and virus-like particles by scanning electron and scanning transmission electron microscopes.

    Kąkol, Monika / Tagliasacchi, Ezher / Borkowski, Andrzej / Słowakiewicz, Mirosław

    Frontiers in microbiology

    2023  Volume 14, Page(s) 1279720

    Abstract: Scanning electron microscopy (SEM) and scanning transmission electron microscopy (STEM) were applied in many laboratories to visualize and image viruses and virus-like particles (VLPs). Two bacteriophages, P1 and Φ6, were chosen as model microorganisms ... ...

    Abstract Scanning electron microscopy (SEM) and scanning transmission electron microscopy (STEM) were applied in many laboratories to visualize and image viruses and virus-like particles (VLPs). Two bacteriophages, P1 and Φ6, were chosen as model microorganisms known for their distinct structure, and viruses obtained from biofilms associated with modern travertines (Terme di Saturnia, Italy; Karahayıt "Kızılsu" and Pamukkale, Turkey) were also investigated. Three protocols, (1)
    Language English
    Publishing date 2023-11-14
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2023.1279720
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  2. Article ; Online: Strengthening Digital Health Technology Capacity in Navajo Communities to Help Counter the COVID-19 Pandemic.

    Begay, Melissa / Kakol, Monika / Sood, Akshay / Upson, Dona

    Annals of the American Thoracic Society

    2021  Volume 18, Issue 7, Page(s) 1109–1114

    MeSH term(s) Biomedical Technology ; COVID-19 ; Humans ; Pandemics ; SARS-CoV-2 ; Telemedicine
    Language English
    Publishing date 2021-03-25
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2717461-X
    ISSN 2325-6621 ; 1943-5665 ; 2325-6621
    ISSN (online) 2325-6621 ; 1943-5665
    ISSN 2325-6621
    DOI 10.1513/AnnalsATS.202009-1136PS
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5828: Show issues Location:
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  3. Article ; Online: Standardization of the protocol for oral cavity examination and collecting of the biological samples for microbiome research using the next-generation sequencing (NGS): own experience with the COVID-19 patients.

    Brzychczy-Sroka, Barbara / Talaga-Ćwiertnia, Katarzyna / Sroka-Oleksiak, Agnieszka / Gurgul, Artur / Zarzecka-Francica, Elżbieta / Ostrowski, Wojciech / Kąkol, Janusz / Drożdż, Kamil / Brzychczy-Włoch, Monika / Zarzecka, Joanna

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 3717

    Abstract: To date, publications have shown that compositions of oral microbiota differ depending on their habitats (e.g. tongue, tonsils, pharynx). The absence of set standards for the choice of the areas and conditions of material collection makes the oral ... ...

    Abstract To date, publications have shown that compositions of oral microbiota differ depending on their habitats (e.g. tongue, tonsils, pharynx). The absence of set standards for the choice of the areas and conditions of material collection makes the oral microbiome one of the most difficult environments for a comparative analysis with other researchers, which is a meaningful limitation during an assessment of the potential effects of microorganisms as biomarkers in the courses of various human diseases. Therefore, standardisation of basic conditions of a dental examination and collection of material for the next generation sequencing (NGS) is worth attempting. The standardisation of the dental exam and collection of the clinical materials: saliva, swab from the tongue ridge, hard palate, palatine tonsils and oropharynx, supragingival plaque and subgingival plaque. Protocol involved the patients (n = 60), assigned to 3 groups: I-COVID-19 convalescents who received antibiotics, n = 17, II-COVID-19 convalescents, n = 23 and III-healthy individuals, n = 20. The collected biological samples were used to conduct NGS (16S rRNA). The conditions of patient preparation for collecting biological materials as well as the schedule of dental examination, were proposed. Based on the research conducted, we have indicated the dental indicators that best differentiate the group of COVID-19 patients (groups I and II) from healthy people (group III). These include the DMFT, D and BOP indices. The use of alpha and beta diversity analysis provided an overall insight into the diversity of microbial communities between specific niches and patient groups. The most different diversity between the studied group of patients (group II) and healthy people (group III) was noted in relation to the supragingival plaque. The order of activities during the dental exam as well as while collecting and securing clinical materials is particularly important to avoid technical errors and material contamination which may result in erroneous conclusions from the analyses of the results of sensitive tests such as the NGS. It has been shown that the dental indices: DMFT, D number, PI and BOP are the best prognostic parameters to assess the oral health. Based on beta diversity the most sensitive niche and susceptible to changes in the composition of the microbiota is the supragingival plaque. The procedures developed by our team can be applied as ready-to-use forms in studies conducted by other researchers.
    MeSH term(s) Humans ; RNA, Ribosomal, 16S/genetics ; COVID-19/diagnosis ; Microbiota/genetics ; Mouth ; High-Throughput Nucleotide Sequencing ; Reference Standards
    Chemical Substances RNA, Ribosomal, 16S
    Language English
    Publishing date 2024-02-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-53992-3
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  4. Article ; Online: Susceptibility of Southwestern American Indian Tribes to Coronavirus Disease 2019 (COVID-19).

    Kakol, Monika / Upson, Dona / Sood, Akshay

    The Journal of rural health : official journal of the American Rural Health Association and the National Rural Health Care Association

    2020  Volume 37, Issue 1, Page(s) 197–199

    MeSH term(s) COVID-19/ethnology ; Comorbidity ; Humans ; Indians, North American/statistics & numerical data ; Pandemics ; SARS-CoV-2 ; Social Determinants of Health/ethnology ; United States/epidemiology ; United States Indian Health Service/statistics & numerical data
    Keywords covid19
    Language English
    Publishing date 2020-06-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 639160-6
    ISSN 1748-0361 ; 0890-765X
    ISSN (online) 1748-0361
    ISSN 0890-765X
    DOI 10.1111/jrh.12451
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    In stock of ZB MED Cologne/Königswinter

    Zs.B 3102: Show issues Location:
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  5. Article: Oral microbiota study of the patients after hospitalisation for COVID-19, considering selected dental indices and antibiotic therapy using the next generation sequencing method (NGS).

    Brzychczy-Sroka, Barbara / Talaga-Ćwiertnia, Katarzyna / Sroka-Oleksiak, Agnieszka / Gurgul, Artur / Zarzecka-Francica, Elżbieta / Ostrowski, Wojciech / Kąkol, Janusz / Zarzecka, Joanna / Brzychczy-Włoch, Monika

    Journal of oral microbiology

    2023  Volume 15, Issue 1, Page(s) 2264591

    Abstract: Background: Poor oral hygiene and the increased incidence and severity of periodontitis may exacerbate SARS-CoV-2 infection. The aim was to evaluate the oral microbiota of 60 participants divided into groups: COVID-19 convalescents who received ... ...

    Abstract Background: Poor oral hygiene and the increased incidence and severity of periodontitis may exacerbate SARS-CoV-2 infection. The aim was to evaluate the oral microbiota of 60 participants divided into groups: COVID-19 convalescents who received antibiotics during hospitalization (I), COVID-19 convalescents without antibiotic therapy (II) and healthy individuals (III).
    Materials and methods: Dental examination was conducted, and oral health status was evaluated using selected dental indexes. Clinical samples (saliva, dorsal swabs, supragingival and subgingival plaque) were collected and used for metagenomic library to the next-generation sequencing (NGS) preparation.
    Results: Each of the clinical materials in particular groups of patients showed a statistically significant and quantitatively different bacterial composition. Patients from group I showed significantly worse oral health, reflected by higher average values of dental indexes and also a higher percentage of Veillonella, Tannerella, Capnocytophaga and Selenomonas genera in comparison to other groups. Additionally, a statistically significant decrease in the amount of Akkermansia type in both groups with COVID-19 was observed for all materials.
    Conclusions: The primary factor affecting the composition of oral microbiota was not the SARS-CoV-2 infection itself, but the use of antibiotic therapy. The increased percentage of pro-inflammatory pathogens observed in COVID-19 patients underscores the importance of preventing periodontal disease and improving oral hygiene in the future.
    Language English
    Publishing date 2023-10-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2523919-3
    ISSN 2000-2297
    ISSN 2000-2297
    DOI 10.1080/20002297.2023.2264591
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  7. Article ; Online: Susceptibility of Southwestern American Indian Tribes to Coronavirus Disease 2019 (COVID‐19)

    Kakol, Monika / Upson, Dona / Sood, Akshay

    The Journal of Rural Health ; ISSN 0890-765X 1748-0361

    2020  

    Keywords Public Health, Environmental and Occupational Health ; covid19
    Language English
    Publisher Wiley
    Publishing country us
    Document type Article ; Online
    DOI 10.1111/jrh.12451
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article: Real-world experience with riociguat as potential bridging therapy in patients with chronic thromboembolic pulmonary hypertension: a case series.

    Balasubramanian, Vijay P / Beutner, Matthew / Gill, Kirat / Kakol, Monika / Melendres-Groves, Lana

    Pulmonary circulation

    2020  Volume 10, Issue 2, Page(s) 2045894019898377

    Language English
    Publishing date 2020-04-16
    Publishing country United States
    Document type Case Reports
    ZDB-ID 2638089-4
    ISSN 2045-8940 ; 2045-8932
    ISSN (online) 2045-8940
    ISSN 2045-8932
    DOI 10.1177/2045894019898377
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  9. Article ; Online: Methicillin-resistant Staphylococcus aureus Nasal Screening Adds Limited Value to the Choice of Empiric Antibiotics in Community-acquired Pneumonia.

    Burnham, Jason P / Kakol, Monika K / Vazquez Guillamet, M Cristina

    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

    2018  Volume 68, Issue 7, Page(s) 1251

    MeSH term(s) Anti-Bacterial Agents ; Antimicrobial Stewardship ; Community-Acquired Infections ; Humans ; Methicillin ; Methicillin-Resistant Staphylococcus aureus ; Pneumonia, Staphylococcal
    Chemical Substances Anti-Bacterial Agents ; Methicillin (Q91FH1328A)
    Language English
    Publishing date 2018-10-30
    Publishing country United States
    Document type Letter ; Comment
    ZDB-ID 1099781-7
    ISSN 1537-6591 ; 1058-4838
    ISSN (online) 1537-6591
    ISSN 1058-4838
    DOI 10.1093/cid/ciy868
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1505: Show issues Location:
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  10. Article ; Online: Cutaneous manifestations of injectable drug use: hidden secrets.

    Barańska-Rybak, Wioletta / Błażewicz, Izabela / Kąkol, Monika / Roter, Mirosław / Nowicki, Roman

    Cutis

    2014  Volume 93, Issue 4, Page(s) 185–187

    Abstract: Abscesses related to drug use are the most common cutaneous manifestations among injection drug users, often occurring when the veins become less accessible. In these cases, other techniques may be used to administer drugs, such as skin popping ( ... ...

    Abstract Abscesses related to drug use are the most common cutaneous manifestations among injection drug users, often occurring when the veins become less accessible. In these cases, other techniques may be used to administer drugs, such as skin popping (subcutaneous injection) or muscle popping (intramuscular injection). The main risk factors for abscess formation include skin popping, use of unsterilized needles, and injection of speedball (a mixture of cocaine and heroin). We present a case of recurrent abscesses accompanied by fever, hypersomnia alternating with insomnia, diaphoresis, fatigue, recent weight loss, and agitation following subcutaneous injection of a tramadol, opipramol, and clonazepam mixture. Differential diagnoses included pyoderma gangrenosum on the basis of hepatitis C virus, skin lesions connected with human immunodeficiency virus infection, vasculitis, endocarditis, and serotonin syndrome. The patient was treated with oral antibiotics, surgical incision, and drainage of the abscesses, with consequent improvement.
    MeSH term(s) Abscess/etiology ; Abscess/microbiology ; Abscess/therapy ; Clonazepam/administration & dosage ; Clonazepam/adverse effects ; Diagnosis, Differential ; Humans ; Male ; Opipramol/administration & dosage ; Opipramol/adverse effects ; Recurrence ; Risk Factors ; Skin Diseases, Bacterial/etiology ; Skin Diseases, Bacterial/microbiology ; Skin Diseases, Bacterial/therapy ; Soft Tissue Infections/etiology ; Soft Tissue Infections/microbiology ; Soft Tissue Infections/therapy ; Substance Abuse, Intravenous/complications ; Tramadol/administration & dosage ; Tramadol/adverse effects ; Treatment Outcome ; Young Adult
    Chemical Substances Tramadol (39J1LGJ30J) ; Clonazepam (5PE9FDE8GB) ; Opipramol (D23ZXO613C)
    Language English
    Publishing date 2014-04
    Publishing country United States
    Document type Case Reports ; Journal Article
    ZDB-ID 391840-3
    ISSN 2326-6929 ; 0011-4162 ; 0151-9522
    ISSN (online) 2326-6929
    ISSN 0011-4162 ; 0151-9522
    Database MEDical Literature Analysis and Retrieval System OnLINE

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