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  1. Article ; Online: Expanding Medical Education to Include Substance Use Disorders During Pregnancy and Postpartum: Preliminary Effectiveness of a Pilot Curriculum for Medical Students.

    Martin, Caitlin E / Martinez Telleria, Mariana / Hostetter, Kara / Thumma, Lillia / Edwards, Cherie / Thakkar, Bhushan

    Journal of addiction medicine

    2024  Volume 18, Issue 2, Page(s) 191–193

    Abstract: Objective: To effectively combat the simultaneous overdose and maternal mortality crises, a multimodal approach is needed. The aim of this study is to evaluate the preliminary effectiveness of a pilot, experiential learning, substance use disorder (SUD) ...

    Abstract Objective: To effectively combat the simultaneous overdose and maternal mortality crises, a multimodal approach is needed. The aim of this study is to evaluate the preliminary effectiveness of a pilot, experiential learning, substance use disorder (SUD) curriculum embedded into a third-year medical student obstetrics and gynecology clerkship to improve self-reported confidence in SUD clinical skills.
    Methods: This SUD curriculum was designed and implemented in an outpatient clinic, which provides integrated obstetric, gynecologic, and addiction medicine services for pregnant and parenting people with SUD. Third-year medical students on their obstetrics and gynecology clerkship rotated 1 full day through the OB MOTIVATE clinic between August 2020 and April 2022 and completed this curriculum. Students completed preclinic assignments and in-clinic tasks (eg, practicing SBIRT under supervision: screening, brief intervention, referral to treatment). Paired t tests assessed changes in outcomes, with increasing scores (range 1-5) demonstrating improvement.
    Results: Sixty-three students rotated through the OB MOTIVATE clinic; 57 completed the curriculum and surveys. Results from the self-assessment tools demonstrated significant improvements in confidence in SUD clinical skills, including performing SBIRT (2.46 ± 0.80 vs 4.07 ± 0.59, P < 0.01), motivational interviewing (2.98 ± 0.86 vs 4.16 ± 0.65, P < 0.01), using evidence-based medicine (2.91 ± 1.09 vs 4.23 ± 0.66, P < 0.01), and collecting an SUD history (3.25 ± 1.04 vs 4.35 ± 0.55, P = 0.01).
    Conclusions: The integration of interventional curriculums into medical school and residency programs could be an effective avenue to reinforce addiction knowledge and teach new skills. This practical 1-day pilot curriculum demonstrated preliminary effectiveness at introducing third-year medical students to the complexities of SUD in pregnancy and postpartum. Further investigations of feasible and acceptable SUD educational interventions are warranted.
    MeSH term(s) Pregnancy ; Humans ; Female ; Students, Medical ; Curriculum ; Internship and Residency ; Substance-Related Disorders/therapy ; Postpartum Period
    Language English
    Publishing date 2024-01-10
    Publishing country Netherlands
    Document type Journal Article
    ISSN 1935-3227
    ISSN (online) 1935-3227
    DOI 10.1097/ADM.0000000000001262
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Human lung organoids as a model for respiratory virus replication and countermeasure performance in human hosts.

    Edwards, Caitlin E / Tata, Aleksandra / Baric, Ralph S

    Translational research : the journal of laboratory and clinical medicine

    2022  Volume 250, Page(s) 36–45

    Abstract: Human respiratory viruses induce a wide breadth of disease phenotypes and outcomes of varying severity. Innovative models that recapitulate the human respiratory tract are needed to study such viruses, understand the virus-host interactions underlying ... ...

    Abstract Human respiratory viruses induce a wide breadth of disease phenotypes and outcomes of varying severity. Innovative models that recapitulate the human respiratory tract are needed to study such viruses, understand the virus-host interactions underlying replication and pathogenesis, and to develop effective countermeasures for prevention and treatment. Human organoid models provide a platform to study virus-host interactions in the proximal to distal lung in the absence of a human in vivo model. These cultures fill the niche of a suitable ex vivo model that represents the in vivo lung environment and encapsulates the structure and function of the native human lung.
    MeSH term(s) Humans ; Organoids/pathology ; Lung/pathology ; Virus Replication ; Viruses
    Language English
    Publishing date 2022-07-16
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2246684-8
    ISSN 1878-1810 ; 1532-6543 ; 1931-5244
    ISSN (online) 1878-1810 ; 1532-6543
    ISSN 1931-5244
    DOI 10.1016/j.trsl.2022.07.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: A familial missense variant in the Alzheimer's disease gene SORL1 impairs its maturation and endosomal sorting.

    Fazeli, Elnaz / Child, Daniel D / Bucks, Stephanie A / Stovarsky, Miki / Edwards, Gabrielle / Rose, Shannon E / Yu, Chang-En / Latimer, Caitlin / Kitago, Yu / Bird, Thomas / Jayadev, Suman / Andersen, Olav M / Young, Jessica E

    Acta neuropathologica

    2024  Volume 147, Issue 1, Page(s) 20

    Abstract: The SORL1 gene has recently emerged as a strong Alzheimer's Disease (AD) risk gene. Over 500 different variants have been identified in the gene and the contribution of individual variants to AD development and progression is still largely unknown. Here, ...

    Abstract The SORL1 gene has recently emerged as a strong Alzheimer's Disease (AD) risk gene. Over 500 different variants have been identified in the gene and the contribution of individual variants to AD development and progression is still largely unknown. Here, we describe a family consisting of 2 parents and 5 offspring. Both parents were affected with dementia and one had confirmed AD pathology with an age of onset > 75 years. All offspring were affected with AD with ages at onset ranging from 53 years to 74 years. DNA was available from the parent with confirmed AD and 5 offspring. We identified a coding variant, p.(Arg953Cys), in SORL1 in 5 of 6 individuals affected by AD. Notably, variant carriers had severe AD pathology, and the SORL1 variant segregated with TDP-43 pathology (LATE-NC). We further characterized this variant and show that this Arginine substitution occurs at a critical position in the YWTD-domain of the SORL1 translation product, SORL1. Functional studies further show that the p.R953C variant leads to retention of the SORL1 protein in the endoplasmic reticulum which leads to decreased maturation and shedding of the receptor and prevents its normal endosomal trafficking. Together, our analysis suggests that p.R953C is a pathogenic variant of SORL1 and sheds light on mechanisms of how missense SORL1 variants may lead to AD.
    MeSH term(s) Humans ; Aged ; Alzheimer Disease/genetics ; Gene Frequency ; Genetic Predisposition to Disease ; Membrane Transport Proteins/genetics ; Mutation, Missense ; LDL-Receptor Related Proteins/genetics ; Polymorphism, Single Nucleotide
    Chemical Substances Membrane Transport Proteins ; LDL-Receptor Related Proteins ; SORL1 protein, human
    Language English
    Publishing date 2024-01-20
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1079-0
    ISSN 1432-0533 ; 0001-6322
    ISSN (online) 1432-0533
    ISSN 0001-6322
    DOI 10.1007/s00401-023-02670-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Characterizing heat mitigation strategies utilized by beef processors in the United States.

    Davis, Melissa K / Engle, Terry E / Cadaret, Caitlin N / Cramer, M Caitlin / Bigler, Libby J / Wagner, John J / Edwards-Callaway, Lily N

    Translational animal science

    2021  Volume 6, Issue 1, Page(s) txab231

    Abstract: During lairage at slaughter plants, cattle can be exposed to extreme heat conditions from pen densities and holding pen microclimates. While research outlining heat mitigation strategies used in other sectors of the beef supply chain is available, there ... ...

    Abstract During lairage at slaughter plants, cattle can be exposed to extreme heat conditions from pen densities and holding pen microclimates. While research outlining heat mitigation strategies used in other sectors of the beef supply chain is available, there is no published data on the use of heat mitigation strategies at slaughter plants. The objective of this study was to characterize short-term heat mitigation strategies used by commercial beef slaughter plants in the United States. Twenty-one beef slaughter plants, representing an estimated 60% of beef slaughter in the United States, were included in the study. All plants indicated use of at least one heat mitigation strategy, and five of them used more than one type. Sprinklers/misters were the most commonly used heat mitigation type (
    Language English
    Publishing date 2021-12-22
    Publishing country England
    Document type Journal Article
    ISSN 2573-2102
    ISSN (online) 2573-2102
    DOI 10.1093/tas/txab231
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: A familial missense variant in the Alzheimer's Disease gene

    Fazeli, Elnaz / Child, Daniel D / Bucks, Stephanie A / Stovarsky, Miki / Edwards, Gabrielle / Rose, Shannon E / Yu, Chang-En / Latimer, Caitlin / Kitago, Yu / Bird, Thomas / Jayadev, Suman / Andersen, Olav M / Young, Jessica E

    bioRxiv : the preprint server for biology

    2023  

    Abstract: ... ...

    Abstract The
    Language English
    Publishing date 2023-11-13
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.07.01.547348
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: NIH Music-Based Intervention Toolkit: Music-Based Interventions for Brain Disorders of Aging.

    Edwards, Emmeline / St Hillaire-Clarke, Coryse / Frankowski, David W / Finkelstein, Robert / Cheever, Thomas / Chen, Wen G / Onken, Lisa / Poremba, Amy / Riddle, Robert / Schloesser, Dana / Burgdorf, Caitlin E / Wells, Nena / Fleming, Renée / Collins, Francis S

    Neurology

    2023  Volume 100, Issue 18, Page(s) 868–878

    Abstract: Music-based interventions (MBIs) show promise for managing symptoms of various brain disorders. To fully realize the potential of MBIs and dispel the outdated misconception that MBIs are rooted in soft science, the NIH is promoting rigorously designed, ... ...

    Abstract Music-based interventions (MBIs) show promise for managing symptoms of various brain disorders. To fully realize the potential of MBIs and dispel the outdated misconception that MBIs are rooted in soft science, the NIH is promoting rigorously designed, well-powered MBI clinical trials. The pressing need of guidelines for scientifically rigorous studies with enhanced data collection brought together the Renée Fleming Foundation, the Foundation for the NIH, the Trans-NIH Music and Health Working Group, and an interdisciplinary scientific expert panel to create the NIH MBI Toolkit for research on music and health across the lifespan. The Toolkit defines the building blocks of MBIs, including a consolidated set of common data elements for MBI protocols, and core datasets of outcome measures and biomarkers for brain disorders of aging that researchers may select for their studies. Utilization of the guiding principles in this Toolkit will be strongly recommended for NIH-funded studies of MBIs.
    MeSH term(s) Humans ; Music ; Mindfulness/methods ; Brain Diseases ; Data Collection ; Aging
    Language English
    Publishing date 2023-01-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 207147-2
    ISSN 1526-632X ; 0028-3878
    ISSN (online) 1526-632X
    ISSN 0028-3878
    DOI 10.1212/WNL.0000000000206797
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Estimation of SARS-CoV-2 Seroprevalence in Central North Carolina: Accounting for Outcome Misclassification in Complex Sample Designs.

    Vias, Nishma P / Cassidy, Caitlin A / Edwards, Jessie K / Xiong, Khou / Beatty Parker, Cherese / Aiello, Allison E / Boyce, Ross M / Shook-Sa, Bonnie E

    Epidemiology (Cambridge, Mass.)

    2023  Volume 34, Issue 5, Page(s) 721–731

    Abstract: Background: Population-based seroprevalence studies are crucial to understand community transmission of COVID-19 and guide responses to the pandemic. Seroprevalence is typically measured from diagnostic tests with imperfect sensitivity and specificity. ... ...

    Abstract Background: Population-based seroprevalence studies are crucial to understand community transmission of COVID-19 and guide responses to the pandemic. Seroprevalence is typically measured from diagnostic tests with imperfect sensitivity and specificity. Failing to account for measurement error can lead to biased estimates of seroprevalence. Methods to adjust seroprevalence estimates for the sensitivity and specificity of the diagnostic test have largely focused on estimation in the context of convenience sampling. Many existing methods are inappropriate when data are collected using a complex sample design.
    Methods: We present methods for seroprevalence point estimation and confidence interval construction that account for imperfect test performance for use with complex sample data. We apply these methods to data from the Chatham County COVID-19 Cohort (C4), a longitudinal seroprevalence study conducted in central North Carolina. Using simulations, we evaluate bias and confidence interval coverage for the proposed estimator compared with a standard estimator under a stratified, three-stage cluster sample design.
    Results: We obtained estimates of seroprevalence and corresponding confidence intervals for the C4 study. SARS-CoV-2 seroprevalence increased rapidly from 10.4% in January to 95.6% in July 2021 in Chatham County, North Carolina. In simulation, the proposed estimator demonstrates desirable confidence interval coverage and minimal bias under a wide range of scenarios.
    Conclusion: We propose a straightforward method for producing valid estimates and confidence intervals when data are based on a complex sample design. The method can be applied to estimate the prevalence of other infections when estimates of test sensitivity and specificity are available.
    MeSH term(s) Humans ; SARS-CoV-2 ; COVID-19/epidemiology ; Seroepidemiologic Studies ; North Carolina/epidemiology ; Bias ; Antibodies, Viral
    Chemical Substances Antibodies, Viral
    Language English
    Publishing date 2023-07-31
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1053263-8
    ISSN 1531-5487 ; 1044-3983
    ISSN (online) 1531-5487
    ISSN 1044-3983
    DOI 10.1097/EDE.0000000000001625
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Impacts of shade on cattle well-being in the beef supply chain.

    Edwards-Callaway, Lily N / Cramer, M Caitlin / Cadaret, Caitlin N / Bigler, Elizabeth J / Engle, Terry E / Wagner, John J / Clark, Daniel L

    Journal of animal science

    2020  Volume 99, Issue 2

    Abstract: Shade is a mechanism to reduce heat load providing cattle with an environment supportive of their welfare needs. Although heat stress has been extensively reviewed, researched, and addressed in dairy production systems, it has not been investigated in ... ...

    Abstract Shade is a mechanism to reduce heat load providing cattle with an environment supportive of their welfare needs. Although heat stress has been extensively reviewed, researched, and addressed in dairy production systems, it has not been investigated in the same manner in the beef cattle supply chain. Like all animals, beef cattle are susceptible to heat stress if they are unable to dissipate heat during times of elevated ambient temperatures. There are many factors that impact heat stress susceptibility in beef cattle throughout the different supply chain sectors, many of which relate to the production system, that is, availability of shade, microclimate of environment, and nutrition management. The results from studies evaluating the effects of shade on production and welfare are difficult to compare due to variation in structural design, construction materials used, height, shape, and area of shade provided. Additionally, depending on operation location, shade may or may not be beneficial during all times of the year, which can influence the decision to make shade a permanent part of management systems. Shade has been shown to lessen the physiologic response of cattle to heat stress. Shaded cattle exhibit lower respiration rates, body temperatures, and panting scores compared with unshaded cattle in weather that increases the risk of heat stress. Results from studies investigating the provision of shade indicate that cattle seek shade in hot weather. The impact of shade on behavioral patterns is inconsistent in the current body of research, with some studies indicating that shade provision impacts behavior and other studies reporting no difference between shaded and unshaded groups. Analysis of performance and carcass characteristics across feedlot studies demonstrated that shaded cattle had increased ADG, improved feed efficiency, HCW, and dressing percentage when compared with cattle without shade. Despite the documented benefits of shade, current industry statistics, although severely limited in scope, indicate low shade implementation rates in feedlots and data in other supply chain sectors do not exist. Industry guidelines and third-party on-farm certification programs articulate the critical need for protection from extreme weather but are not consistent in providing specific recommendations and requirements. Future efforts should include: updated economic analyses of cost vs. benefit of shade implementation, exploration of producer perspectives and needs relative to shade, consideration of shade impacts in the cow-calf and slaughter plant segments of the supply chain, and integration of indicators of affective (mental) state and preference in research studies to enhance the holistic assessment of cattle welfare.
    MeSH term(s) Animals ; Body Temperature ; Cattle ; Cattle Diseases ; Female ; Heat Stress Disorders/veterinary ; Heat-Shock Response ; Respiratory Rate ; Sunlight
    Language English
    Publishing date 2020-11-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 390959-1
    ISSN 1525-3163 ; 0021-8812
    ISSN (online) 1525-3163
    ISSN 0021-8812
    DOI 10.1093/jas/skaa375
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: From 'no problem' to 'a lot of difficulties': barriers to health service utilization among migrants in Rakai, Uganda.

    Young, Ruth / Kennedy, Caitlin E / Dam, Anita / Nakyanjo, Neema / Ddaaki, William / Kiyingi, Anne Catherine / Mukwana, Emmanuel / Edwards, Abagail / Nalugoda, Fred / Chang, Larry W / Wawer, Maria J / Oaks, Maya / Paina, Ligia

    Health policy and planning

    2023  Volume 38, Issue 5, Page(s) 620–630

    Abstract: Migration is increasingly common in Africa, especially for employment. Migrants may face additional barriers to accessing health care, including human immunodeficiency virus (HIV) prevention and treatment, compared with long-term residents. Exploring ... ...

    Abstract Migration is increasingly common in Africa, especially for employment. Migrants may face additional barriers to accessing health care, including human immunodeficiency virus (HIV) prevention and treatment, compared with long-term residents. Exploring migrants' experiences with health services can provide insights to inform the design of health programmes. In this study, we used qualitative methods to understand migrants' barriers to health service utilization in south-central Uganda. This secondary data analysis used data from in-depth semi-structured interviews with 35 migrants and 25 key informants between 2017 and 2021. Interviews were analysed thematically through team debriefings and memos. We constructed three representative migrant journeys to illustrate barriers to accessing health services, reflecting experiences of migrant personas with differing HIV status and wealth. Migrants reported experiencing a range of barriers, which largely depended on the resources they could access, their existing health needs and their ability to form connections and relationships at their destination. Migrants were less familiar with local health services, and sometimes needed more time and resources to access care. Migrants living with HIV faced additional barriers to accessing health services due to anticipated discrimination from community members or health workers and difficulties in continuing antiretroviral therapy when switching health facilities. Despite these barriers, social networks and local connections facilitated access. However, for some migrants, such as those who were poorer or living with HIV, these barriers were more pronounced. Our work highlights how local connections with community members and health workers help migrants access health services. In practice, reducing barriers to health services is likely to benefit both migrants and long-term residents.
    MeSH term(s) Humans ; Transients and Migrants ; Uganda ; Health Services Accessibility ; Health Services ; HIV Infections/drug therapy ; HIV Infections/prevention & control
    Language English
    Publishing date 2023-03-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 632896-9
    ISSN 1460-2237 ; 0268-1080
    ISSN (online) 1460-2237
    ISSN 0268-1080
    DOI 10.1093/heapol/czad019
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Prolonged airway explant culture enables study of health, disease, and viral pathogenesis.

    Lee-Ferris, Rhianna E / Okuda, Kenichi / Galiger, Jacob R / Schworer, Stephen A / Rogers, Troy D / Dang, Hong / Gilmore, Rodney / Edwards, Caitlin / Nakano, Satoko / Cawley, Anne M / Pickles, Raymond J / Gallant, Samuel C / Crisci, Elisa / Rivier, Lauraine / Hagood, James S / O'Neal, Wanda K / Baric, Ralph S / Grubb, Barbara R / Boucher, Richard C /
    Randell, Scott H

    bioRxiv : the preprint server for biology

    2024  

    Abstract: In vitro models play a major role in studying airway physiology and disease. However, the native lung's complex tissue architecture and non-epithelial cell lineages are not preserved in these models. Ex vivo tissue models could overcome in vitro ... ...

    Abstract In vitro models play a major role in studying airway physiology and disease. However, the native lung's complex tissue architecture and non-epithelial cell lineages are not preserved in these models. Ex vivo tissue models could overcome in vitro limitations, but methods for long-term maintenance of ex vivo tissue has not been established. We describe methods to culture human large airway explants, small airway explants, and precision-cut lung slices for at least 14 days. Human airway explants recapitulate genotype-specific electrophysiology, characteristic epithelial, endothelial, stromal and immune cell populations, and model viral infection after 14 days in culture. These methods also maintain mouse, rabbit, and pig tracheal explants. Notably, intact airway tissue can be cryopreserved, thawed, and used to generate explants with recovery of function 14 days post-thaw. These studies highlight the broad applications of airway tissue explants and their use as translational intermediates between in vitro and in vivo studies.
    Language English
    Publishing date 2024-02-05
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.02.03.578756
    Database MEDical Literature Analysis and Retrieval System OnLINE

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