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  1. Article: Comparison of Tongue Characteristics Classified According to Ultrasonographic Features Using a K-Means Clustering Algorithm.

    Chantaramanee, Ariya / Nakagawa, Kazuharu / Yoshimi, Kanako / Nakane, Ayako / Yamaguchi, Kohei / Tohara, Haruka

    Diagnostics (Basel, Switzerland)

    2022  Volume 12, Issue 2

    Abstract: ... function by classifying ultrasound images of the tongue using a K-means clustering algorithm. During 2017 ... using K-means clustering may aid clinicians in selecting the appropriate treatment strategy. Indeed ...

    Abstract The precise correlations among tongue function and characteristics remain unknown, and no previous studies have attempted machine learning-based classification of tongue ultrasonography findings. This cross-sectional observational study aimed to investigate relationships among tongue characteristics and function by classifying ultrasound images of the tongue using a K-means clustering algorithm. During 2017-2018, 236 healthy older participants (mean age 70.8 ± 5.4 years) were enrolled. The optimal number of clusters determined by the elbow method was 3. After analysis of tongue thickness and echo intensity plots, tongues were classified into three groups. One-way ANOVA was used to compare tongue function, tongue pressure, and oral diadochokinesis for /ta/ and /ka/ in each group. There were significant differences in all tongue functions among the three groups. The worst function was observed in patients with the lowest values for tongue thickness and echo intensity (tongue pressure [P = 0.023], /ta/ [P = 0.007], and /ka/ [P = 0.038]). Our results indicate that ultrasonographic classification of tongue characteristics using K-means clustering may aid clinicians in selecting the appropriate treatment strategy. Indeed, ultrasonography is advantageous in that it provides real-time imaging that is non-invasive, which can improve patient follow-up both in the clinic and at home.
    Language English
    Publishing date 2022-01-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics12020264
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Expression of cathepsins B, D and K in thymic epithelial tumours.

    Yamaguchi, Naoko / Tomaru, Utano / Kiuchi, Takayuki / Ishizu, Akihiro / Deguchi, Takahiro / Otsuka, Noriyuki / Tanaka, Satoshi / Marukawa, Katsuji / Matsuno, Yoshihiro / Kitagawa, Masanobu / Kasahara, Masanori

    Journal of clinical pathology

    2020  Volume 74, Issue 2, Page(s) 84–90

    Abstract: ... immunohistochemical analysis of cathepsin B (CTB), cathepsin D (CTD) and cathepsin K (CTK), all of which have been ...

    Abstract Aim: Cathepsins are proteases that regulate a wide range of physiological processes, including protein turnover, cell signalling and antigen presentation. Recent studies have shown that cathepsins are highly upregulated in many types of tumours. Of the 15 cathepsins in humans, cathepsins V and S are abundantly expressed in the thymus, and we previously showed that the immunostaining of these cathepsins could serve as diagnostic markers for thymic epithelial tumours. However, little is known about the expression of other cathepsins in thymic epithelial tumours. To determine the diagnostic implications of cathepsins, we performed immunohistochemical analysis of cathepsin B (CTB), cathepsin D (CTD) and cathepsin K (CTK), all of which have been reported to correlate with the progression of squamous cell carcinoma.
    Methods: The association between cathepsin expression and clinicopathological features was evaluated in 122 cases of thymoma and thymic carcinoma.
    Results: CTB and CTD were frequently expressed in type A and type AB thymomas. In contrast, CTB and CTD were significantly less common in type B thymomas than in type A or AB thymomas. In type AB thymomas, the expression of CTB correlated with histological features, and was found predominantly in the type A component. Notably, CTK was expressed most commonly in thymic carcinomas, and patients who died of the disease showed increased expression of CTK.
    Conclusions: The expression of CTB and CTD correlated with the histological subtype of thymoma. In addition, the expression of CTK appears to be useful for the diagnosis of thymic carcinomas and as a prognostic marker.
    MeSH term(s) Adolescent ; Adult ; Aged ; Aged, 80 and over ; Biomarkers, Tumor/analysis ; Biomarkers, Tumor/metabolism ; Cathepsin B/biosynthesis ; Cathepsin D/biosynthesis ; Cathepsin K/biosynthesis ; Female ; Humans ; Male ; Middle Aged ; Neoplasms, Glandular and Epithelial/metabolism ; Neoplasms, Glandular and Epithelial/pathology ; Thymus Neoplasms/metabolism ; Thymus Neoplasms/pathology ; Young Adult
    Chemical Substances Biomarkers, Tumor ; CTSB protein, human (EC 3.4.22.1) ; Cathepsin B (EC 3.4.22.1) ; CTSK protein, human (EC 3.4.22.38) ; Cathepsin K (EC 3.4.22.38) ; CTSD protein, human (EC 3.4.23.5) ; Cathepsin D (EC 3.4.23.5)
    Language English
    Publishing date 2020-05-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 80261-x
    ISSN 1472-4146 ; 0021-9746
    ISSN (online) 1472-4146
    ISSN 0021-9746
    DOI 10.1136/jclinpath-2020-206551
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Enhanced BMP signaling in Cathepsin K-positive tendon progenitors induces heterotopic ossification.

    Yamaguchi, Hiroyuki / Li, Margaret / Kitami, Megumi / Swaminathan, Sowmya / Mishina, Yuji / Komatsu, Yoshihiro

    Biochemical and biophysical research communications

    2023  Volume 688, Page(s) 149147

    Abstract: ... in Cathepsin K-Cre labeled tendon progenitors (hereafter "Ca-Alk2:Ctsk-Cre"). Ca-Alk2:Ctsk-Cre mice were viable ...

    Abstract Heterotopic ossification (HO) is abnormal bone growth in soft tissues that results from injury, trauma, and rare genetic disorders. Bone morphogenetic proteins (BMPs) are critical osteogenic regulators which are involved in HO. However, it remains unclear how BMP signaling interacts with other extracellular stimuli to form HO. To address this question, using the Cre-loxP recombination system in mice, we conditionally expressed the constitutively activated BMP type I receptor ALK2 with a Q207D mutation (Ca-ALK2) in Cathepsin K-Cre labeled tendon progenitors (hereafter "Ca-Alk2:Ctsk-Cre"). Ca-Alk2:Ctsk-Cre mice were viable but they formed spontaneous HO in the Achilles tendon. Histological and molecular marker analysis revealed that HO is formed via endochondral ossification. Ectopic chondrogenesis coincided with enhanced GLI1 production, suggesting that elevated Hedgehog (Hh) signaling is involved in the pathogenesis of HO. Interestingly, focal adhesion kinase, a critical mediator for the mechanotransduction pathway, was also activated in Ca-Alk2:Ctsk-Cre mice. Our findings suggest that enhanced BMP signaling may elevate Hh and mechanotransduction pathways, thereby causing HO in the regions of the Achilles tendon.
    MeSH term(s) Mice ; Animals ; Cathepsin K/metabolism ; Mechanotransduction, Cellular ; Hedgehog Proteins ; Ossification, Heterotopic/metabolism ; Tendons/metabolism
    Chemical Substances Cathepsin K (EC 3.4.22.38) ; Hedgehog Proteins
    Language English
    Publishing date 2023-10-24
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 205723-2
    ISSN 1090-2104 ; 0006-291X ; 0006-291X
    ISSN (online) 1090-2104 ; 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2023.149147
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Characterization of rice KT/HAK/KUP potassium transporters and K

    Okada, Tomoyuki / Yamane, Sousuke / Yamaguchi, Masatoshi / Kato, Ko / Shinmyo, Atsuhiko / Tsunemitsu, Yuta / Iwasaki, Kozo / Ueno, Daisei / Demura, Taku

    Plant biotechnology (Tokyo, Japan)

    2019  Volume 35, Issue 2, Page(s) 101–111

    Abstract: Plant high-affinity K ...

    Abstract Plant high-affinity K
    Language English
    Publishing date 2019-12-02
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 2210142-1
    ISSN 1347-6114 ; 1342-4580
    ISSN (online) 1347-6114
    ISSN 1342-4580
    DOI 10.5511/plantbiotechnology.18.0308a
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Latent pathogenicity of the G38S polymorphism of KCNE1 K

    Yamaguchi, Yoshiaki / Mizumaki, Koichi / Hata, Yukiko / Sakamoto, Tamotsu / Nakatani, Yosuke / Kataoka, Naoya / Ichida, Fukiko / Inoue, Hiroshi / Nishida, Naoki

    Heart and vessels

    2017  Volume 32, Issue 2, Page(s) 186–192

    Abstract: KCNE1 encodes a modulator of KCNQ1 and KCNH2 channels. Although KCNE1(G38S), a single-nucleotide polymorphism (SNP) causing a G38S substitution in KCNE1, is found frequently, whether and how this SNP causes long QT syndrome (LQTS) remains unclear. We ... ...

    Abstract KCNE1 encodes a modulator of KCNQ1 and KCNH2 channels. Although KCNE1(G38S), a single-nucleotide polymorphism (SNP) causing a G38S substitution in KCNE1, is found frequently, whether and how this SNP causes long QT syndrome (LQTS) remains unclear. We evaluated rate-dependent repolarization dynamics using Holter electrocardiogram (ECG) to assess the pathogenicity of KCNE1(G38S). Forty-five patients exhibiting long QT intervals, as assessed by their baseline ECGs, and 16 control subjects were enrolled. KCNE1(G38S) carriers were identified using genome sequencing. LQTS patients were classified into LQT1 or LQT2 using genetic analysis or epinephrine test. QT-RR relations were determined using 24-h Holter ECG recordings. Among the 15 patients (33.3 %) with KCNE1(G38S), four patients without any mutations or amino acid changes in other major cardiac ion channels were categorized as KCNE1(G38S) carriers. In the QT-RR regression lines, the QT-RR slope was greater in the KCNE1(G38S) carriers and the LQT2 patients (0.215 ± 0.021 and 0.207 ± 0.032, respectively) than in the LQT1 patients (0.163 ± 0.014, P < 0.05) and the control subjects (0.135 ± 0.025, P < 0.001). The calculated QT intervals at an RR interval of 1200 ms were longer in the KCNE1(G38S) carriers and LQT1 and LQT2 patients than in the control subjects. Patients with KCNE1(G38S) had a rate-dependent repolarization abnormality similar to patients with LQT2 and, therefore, may have a potential risk to develop lethal arrhythmias.
    MeSH term(s) Adolescent ; Case-Control Studies ; Child ; Electrocardiography, Ambulatory ; Female ; Genetic Predisposition to Disease ; Heterozygote ; Humans ; Linear Models ; Long QT Syndrome/diagnosis ; Long QT Syndrome/genetics ; Male ; Mutation ; Polymorphism, Single Nucleotide ; Potassium Channels, Voltage-Gated/genetics ; Young Adult
    Chemical Substances KCNE1 protein, human ; Potassium Channels, Voltage-Gated
    Language English
    Publishing date 2017-02
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 89678-0
    ISSN 1615-2573 ; 0910-8327 ; 0935-736X
    ISSN (online) 1615-2573
    ISSN 0910-8327 ; 0935-736X
    DOI 10.1007/s00380-016-0859-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Relationship between serum estradiol, cathepsin K, and N-telopeptide of type I collagen in female dogs.

    Yamaguchi, Fumi / Nishi, Hiroshi / Kuramoto, Tomohide / Saitoh, Yasuo / Sogawa, Takeshi / Misumi, Kazuhiro / Fujiki, Makoto

    Research in veterinary science

    2020  Volume 130, Page(s) 133–138

    Abstract: ... years old) and analyzed for serum E2, osteoclast-secreted cysteine protease cathepsin K (CTK), and N ...

    Abstract Ovariohysterectomized (OHE) female dogs do not develop the osteopenia and osteoporosis associated with decreasing estrogen in post-menopausal women, possibly due to post-OHE bone mineral density retention through a mechanism that remains unclear. In this study, we aimed to elucidate this mechanism by investigating estradiol (E2) and bone markers. Samples were collected from 56 OHE and 43 intact bitches (0.33 to 17.58 years old) and analyzed for serum E2, osteoclast-secreted cysteine protease cathepsin K (CTK), and N-telopeptide of type I collagen (NTx) by ELISA. OHE and intact bitches showed no significant difference in serum E2 or NTx, and there was no correlation between serum E2 and NTx and age and time since OHE. Intact bitches showed a very low correlation between E2 and NTx, but OHE bitches showed no correlation, and serum CTK was generally undetectable in both groups. Our findings suggest the influence of gonadal hormones on bone metabolism does not work effectively in dogs; this is consistent with a shorter duration of exposure to E2 in bitches (through the 4-to-8-month anestrus phase) than women.
    MeSH term(s) Animals ; Biomarkers/blood ; Cathepsin K/blood ; Collagen Type I/blood ; Dogs/metabolism ; Estradiol/blood ; Female ; Hysterectomy/veterinary ; Ovariectomy/veterinary ; Peptides/blood ; Retrospective Studies
    Chemical Substances Biomarkers ; Collagen Type I ; Peptides ; collagen type I trimeric cross-linked peptide ; Estradiol (4TI98Z838E) ; Cathepsin K (EC 3.4.22.38)
    Language English
    Publishing date 2020-03-07
    Publishing country England
    Document type Journal Article
    ZDB-ID 840961-4
    ISSN 1532-2661 ; 0034-5288
    ISSN (online) 1532-2661
    ISSN 0034-5288
    DOI 10.1016/j.rvsc.2020.03.015
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: The discovery and characterization of K-563, a novel inhibitor of the Keap1/Nrf2 pathway produced by Streptomyces sp.

    Hori, Ran / Yamaguchi, Kozo / Sato, Hidetaka / Watanabe, Miwa / Tsutsumi, Kyoko / Iwamoto, Susumu / Abe, Masayuki / Onodera, Hideyuki / Nakamura, Satoshi / Nakai, Ryuichiro

    Cancer medicine

    2019  Volume 8, Issue 3, Page(s) 1157–1168

    Abstract: ... we identified the novel Keap1/Nrf2 pathway inhibitor K-563, which was isolated from actinomycete Streptomyces sp ... K-563 suppressed the expression of Keap1/Nrf2 pathway downstream target genes or the downstream ... in A549 cells. K-563 also inhibited the expression of downstream target genes in other Keap1- or Nrf2 ...

    Abstract Keap1/Nrf2 pathway regulates the antioxidant stress response, detoxification response, and energy metabolism. Previous reports found that aberrant Keap1/Nrf2 pathway activation due to Kelch-like ECH-associated protein 1 (Keap1) mutations or Nuclear factor E2-related factor 2 (Nrf2) mutations induced resistance of cancer cells to chemotherapy and accelerated cell growth via the supply of nutrients. Therefore, Keap1/Nrf2 pathway activation is associated with a poor prognosis in many cancers. These previous findings suggested that inhibition of Keap1/Nrf2 pathway could be a target for anti-cancer therapies. To discover a small-molecule Keap1/Nrf2 pathway inhibitor, we conducted high-throughput screening in Keap1 mutant human lung cancer A549 cells using a transcriptional reporter assay. Through this screening, we identified the novel Keap1/Nrf2 pathway inhibitor K-563, which was isolated from actinomycete Streptomyces sp. K-563 suppressed the expression of Keap1/Nrf2 pathway downstream target genes or the downstream target protein, which induced suppression of GSH production, and activated reactive oxygen species production in A549 cells. K-563 also inhibited the expression of downstream target genes in other Keap1- or Nrf2-mutated cancer cells. Furthermore, K-563 exerted anti-proliferative activities in these mutated cancer cells. These in vitro analyses showed that K-563 was able to inhibit cell growth in Keap1- or Nrf2-mutated cancer cells by Keap1/Nrf2 pathway inhibition. K-563 also exerted synergistic combinational effects with lung cancer chemotherapeutic agents. An in vivo study in mice xenotransplanted with A549 cells to further explore the therapeutic potential of K-563 revealed that it also inhibited Keap1/Nrf2 pathway in lung cancer tumors. K-563, a novel Keap1/Nrf2 pathway inhibitor, may be a lead compound for development as an anti-cancer agent.
    MeSH term(s) Animals ; Antineoplastic Agents/chemistry ; Antineoplastic Agents/isolation & purification ; Antineoplastic Agents/metabolism ; Antineoplastic Agents/pharmacology ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Disease Models, Animal ; Drug Resistance, Neoplasm/drug effects ; Genes, Reporter ; Humans ; Kelch-Like ECH-Associated Protein 1/genetics ; Kelch-Like ECH-Associated Protein 1/metabolism ; Magnetic Resonance Spectroscopy ; Mice ; Molecular Structure ; Mutation ; NF-E2-Related Factor 2/genetics ; NF-E2-Related Factor 2/metabolism ; RNA Interference ; Reactive Oxygen Species/metabolism ; Signal Transduction/drug effects ; Streptomyces/metabolism ; Xenograft Model Antitumor Assays
    Chemical Substances Antineoplastic Agents ; KEAP1 protein, human ; Kelch-Like ECH-Associated Protein 1 ; NF-E2-Related Factor 2 ; Reactive Oxygen Species
    Language English
    Publishing date 2019-02-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2045-7634
    ISSN (online) 2045-7634
    DOI 10.1002/cam4.1949
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Expression of tartrate-resistant acid phosphatase and cathepsin K during osteoclast differentiation in developing mouse mandibles.

    Nakamura, Megumi / Aoyama, Naoki / Yamaguchi, Satoshi / Sasano, Yasuyuki

    Biomedical research (Tokyo, Japan)

    2021  Volume 42, Issue 1, Page(s) 13–21

    Abstract: ... acid phosphatase (TRAP) activity and cathepsin K immunoreactivity to identify the presence of osteoclasts, and ... Cathepsin K-immunopositive cells were detected around the same time as the onset of bone mineralization ... whereas TRAP-positive cells appeared prior to bone mineralization. Cathepsin K protein was expressed only ...

    Abstract The present study was designed to test the hypothesis that osteoclasts appear after or at the same time as the initiation of bone mineralization in developing intramembranous bones. We examined mineral deposition via Von Kossa staining to determine when bone mineralization begins, tartrate-resistant acid phosphatase (TRAP) activity and cathepsin K immunoreactivity to identify the presence of osteoclasts, and their mRNA expression levels to assess osteoclastic differentiation in the embryonic mouse mandible. Cathepsin K-immunopositive cells were detected around the same time as the onset of bone mineralization, whereas TRAP-positive cells appeared prior to bone mineralization. Cathepsin K protein was expressed only in multinucleated osteoclasts, whereas TRAP activity was identified in both mono- and multinucleated cells. During bone development, TRAP-positive cells altered their morphology, which was related to the number of their nuclei. The elevated mRNA levels of TRAP and cathepsin K were consistent with the increased percentage of multinucleated osteoclasts and the progression of bone development. Our study revealed that TRAP-positive cells appear prior to bone mineralization, and TRAP- and cathepsin K-positive multinucleated osteoclasts appear at the same time as the initiation of bone mineralization in embryonic mouse mandibles, suggesting that osteoclasts contribute to bone matrix maturation during intramembranous ossification.
    MeSH term(s) Animals ; Bone Development ; Bone Resorption/metabolism ; Calcification, Physiologic ; Cathepsin K/metabolism ; Cell Differentiation ; Gene Expression Profiling ; Gene Expression Regulation ; Mandible/embryology ; Mandible/growth & development ; Mice ; Mice, Inbred C57BL ; Osteoclasts/cytology ; Osteogenesis ; Tartrate-Resistant Acid Phosphatase/metabolism
    Chemical Substances Tartrate-Resistant Acid Phosphatase (EC 3.1.3.2) ; Cathepsin K (EC 3.4.22.38)
    Language English
    Publishing date 2021-02-09
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 604561-3
    ISSN 1880-313X ; 0388-6107
    ISSN (online) 1880-313X
    ISSN 0388-6107
    DOI 10.2220/biomedres.42.13
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Speciation of phosphorus accumulated in fertilized cropland of Aichi prefecture in Japan with different soil properties by sequential chemical extraction and P K-edge XANES

    Ando, Koori / Yamaguchi, Noriko / Kasuya, Masahiro / Toki, Katsutoshi / Nakamura, Yoshitaka

    Soil science and plant nutrition

    2021  Volume 67, Issue 2, Page(s) 150

    Language English
    Document type Article
    ZDB-ID 406719-8
    ISSN 0038-0768
    Database Current Contents Nutrition, Environment, Agriculture

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  10. Article ; Online: Speciation of phosphorus accumulated in fertilized cropland of Aichi prefecture in Japan with different soil properties by sequential chemical extraction and P K-edge XANES

    Ando, Kaori / Yamaguchi, Noriko / Nakamura, Yoshitaka / Kasuya, Masahiro / Taki, Katsutoshi

    Soil Science and Plant Nutrition. 2021 Mar. 04, v. 67, no. 2 p.150-161

    2021  

    Abstract: ... method), P K-edge X-ray adsorption near-edge structure (XANES). Phosphorus accumulated in cropland ...

    Abstract Fertilizer-derived P is accumulated in cropland soils and their phytoavailability is variable depending on the speciation of P, which is affected by soil properties. In this study, we aimed to clarify how soil properties affect the speciation of P accumulated in fertilized soils. Soil samples were collected from the cultivated soil layers of 137 vegetable fields in Aichi prefecture, Japan. The speciation of soil P was evaluated by Truog method, sequential extraction by acetic acid, NH₄F, and NaOH (Sekiya method), P K-edge X-ray adsorption near-edge structure (XANES). Phosphorus accumulated in cropland consisted predominantly of the inorganic form. Phosphorus extracted by the Truog method and acetic acid was correlated with exchangeable Ca and total C. The XANES analysis indicated that the P species extracted by Truog and acetic acid were associated with Ca, while those extracted by acetic acid also included crystalized calcium phosphate with higher crystallinity than CaHPO₄, for example, hydroxyapatite. Due to the high application of P fertilizer and manure with lime, P was accumulated as calcium phosphate except for soils with relatively high contents of oxalate extractable Al and Fe. The amounts of NH₄F-extracted P were higher in soils with larger amounts of oxalate extractable Al and Fe. Based on the XANES analyses, the P species extracted by NH₄F were associated with Al and Fe, which is inconsistent with the operational definition of NH₄F-soluble species. The P in soils extracted by NaOH was correlated with various soil properties, especially with clay content. The P species extracted by NaOH could not be identified. Our results revealed that the important factors controlling available P associated with Ca were total C and exchangeable Ca, whereas those controlling the P extracted by NH₄F were oxalate extractable Al and Fe, because this fraction of P was associated with Al and Fe.
    Keywords Japan ; X-radiation ; X-ray absorption spectroscopy ; acetic acid ; adsorption ; agricultural soils ; calcium hydrogen phosphate ; clay fraction ; cropland ; crystal structure ; exchangeable calcium ; hydroxyapatite ; oxalates ; phosphorus ; phosphorus fertilizers ; phytoaccumulation ; plant nutrition ; vegetables ; XANES ; sequential fractionation ; soil properties ; excessive P accumulation ; soil P species
    Language English
    Dates of publication 2021-0304
    Size p. 150-161.
    Publishing place Taylor & Francis
    Document type Article ; Online
    Note NAL-AP-2-clean
    ZDB-ID 406719-8
    ISSN 1747-0765 ; 0038-0768
    ISSN (online) 1747-0765
    ISSN 0038-0768
    DOI 10.1080/00380768.2021.1874249
    Database NAL-Catalogue (AGRICOLA)

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