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Article ; Online: Exercise-induced responses in matrix metalloproteinases and osteopontin are not moderated by exercise format in males with overweight or obesity.

Raman, Aaron / Peiffer, Jeremiah J / Hoyne, Gerard F / Lawler, Nathan G / Currie, Andrew / Fairchild, Timothy J

2023 Volume 123, Issue 5, Page(s) 1115–1124

Abstract: Purpose: Matrix metalloproteinase-2 (MMP-2) and -3 (MMP-3), and osteopontin (OPN) are associated with adipose-tissue expansion and development of metabolic disease. The purpose of the current study was to assess the circulating concentration of these ... ...

Abstract	Purpose: Matrix metalloproteinase-2 (MMP-2) and -3 (MMP-3), and osteopontin (OPN) are associated with adipose-tissue expansion and development of metabolic disease. The purpose of the current study was to assess the circulating concentration of these markers, along with adiponectin and glucose concentrations, in response to acute exercise in individuals with overweight or obesity. Methods: Fourteen sedentary males with overweight or obesity (29.0 ± 3.1 kg/m Results: Exercise transiently increased MMP-3 and decreased OPN (both p < 0.01), but not MMP-2 or adiponectin. There were no differences in the response of inflammatory markers to the different exercise formats. Exercise increased mean daily glucose concentration and area under the glucose curve during the OGTT on Day 2 and Day 3 (main effect of time; p < 0.05). Conclusion: Acute cycling exercise decreased OPN, which is consistent with longer term improvements in cardiometabolic health and increased MMP-3, which is consistent with its role in tissue remodelling. Interestingly, exercise performed prior to the morning OGTT augmented the glucose concentrations in males. Trial registration: ACTRN12613001086752.
MeSH term(s)	Male ; Humans ; Overweight/therapy ; Overweight/complications ; Matrix Metalloproteinase 2 ; Matrix Metalloproteinase 3 ; Blood Glucose/metabolism ; Osteopontin ; Adiponectin ; Obesity/therapy ; Obesity/complications ; Exercise/physiology ; Glucose
Chemical Substances	Matrix Metalloproteinase 2 (EC 3.4.24.24) ; Matrix Metalloproteinase 3 (EC 3.4.24.17) ; Blood Glucose ; Osteopontin (106441-73-0) ; Adiponectin ; Glucose (IY9XDZ35W2)
Language	English
Publishing date	2023-01-17
Publishing country	Germany
Document type	Journal Article
ZDB-ID	124793-1
ISSN	1439-6327 ; 1432-1025 ; 0301-5548 ; 1439-6319
ISSN (online)	1439-6327 ; 1432-1025
ISSN	0301-5548 ; 1439-6319
DOI	10.1007/s00421-023-05133-3
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Article: Metabolomics and Lipidomics: Expanding the Molecular Landscape of Exercise Biology.

Belhaj, Mehdi R / Lawler, Nathan G / Hoffman, Nolan J

Metabolites

2021 Volume 11, Issue 3

Abstract: Dynamic changes in circulating and tissue metabolites and lipids occur in response to exercise-induced cellular and whole-body energy demands to maintain metabolic homeostasis. The metabolome and lipidome in a given biological system provides a molecular ...

Abstract	Dynamic changes in circulating and tissue metabolites and lipids occur in response to exercise-induced cellular and whole-body energy demands to maintain metabolic homeostasis. The metabolome and lipidome in a given biological system provides a molecular snapshot of these rapid and complex metabolic perturbations. The application of metabolomics and lipidomics to map the metabolic responses to an acute bout of aerobic/endurance or resistance exercise has dramatically expanded over the past decade thanks to major analytical advancements, with most exercise-related studies to date focused on analyzing human biofluids and tissues. Experimental and analytical considerations, as well as complementary studies using animal model systems, are warranted to help overcome challenges associated with large human interindividual variability and decipher the breadth of molecular mechanisms underlying the metabolic health-promoting effects of exercise. In this review, we provide a guide for exercise researchers regarding analytical techniques and experimental workflows commonly used in metabolomics and lipidomics. Furthermore, we discuss advancements in human and mammalian exercise research utilizing metabolomic and lipidomic approaches in the last decade, as well as highlight key technical considerations and remaining knowledge gaps to continue expanding the molecular landscape of exercise biology.
Language	English
Publishing date	2021-03-07
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2662251-8
ISSN	2218-1989
ISSN	2218-1989
DOI	10.3390/metabo11030151
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Article ; Online: Rapid and Self-Administrable Capillary Blood Microsampling Demonstrates Statistical Equivalence with Standard Venous Collections in NMR-Based Lipoprotein Analysis.

Roberts, Jayden Lee / Whiley, Luke / Gray, Nicola / Gay, Melvin / Nitschke, Philipp / Masuda, Reika / Holmes, Elaine / Nicholson, Jeremy K / Wist, Julien / Lawler, Nathan G

Analytical chemistry

2024 Volume 96, Issue 11, Page(s) 4505–4513

Abstract: We investigated plasma and serum blood derivatives from capillary blood microsamples (500 μL, MiniCollect tubes) and corresponding venous blood (10 mL vacutainers). Samples from 20 healthy participants were analyzed ... ...

Abstract	We investigated plasma and serum blood derivatives from capillary blood microsamples (500 μL, MiniCollect tubes) and corresponding venous blood (10 mL vacutainers). Samples from 20 healthy participants were analyzed by
MeSH term(s)	Humans ; Lipoproteins ; Magnetic Resonance Spectroscopy ; Specimen Handling ; Plasma
Chemical Substances	Lipoproteins
Language	English
Publishing date	2024-02-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.3c05152
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Article ; Online: Cross-Validation of Metabolic Phenotypes in SARS-CoV-2 Infected Subpopulations Using Targeted Liquid Chromatography-Mass Spectrometry (LC-MS).

Whiley, Luke / Lawler, Nathan G / Zeng, Annie Xu / Lee, Alex / Chin, Sung-Tong / Bizkarguenaga, Maider / Bruzzone, Chiara / Embade, Nieves / Wist, Julien / Holmes, Elaine / Millet, Oscar / Nicholson, Jeremy K / Gray, Nicola

Journal of proteome research

2024 Volume 23, Issue 4, Page(s) 1313–1327

Abstract: To ensure biological validity in metabolic phenotyping, findings must be replicated in independent sample sets. Targeted workflows have long been heralded as ideal platforms for such validation due to their robust quantitative capability. We evaluated ... ...

Abstract	To ensure biological validity in metabolic phenotyping, findings must be replicated in independent sample sets. Targeted workflows have long been heralded as ideal platforms for such validation due to their robust quantitative capability. We evaluated the capability of liquid chromatography-mass spectrometry (LC-MS) assays targeting organic acids and bile acids to validate metabolic phenotypes of SARS-CoV-2 infection. Two independent sample sets were collected: (1) Australia: plasma, SARS-CoV-2 positive (
MeSH term(s)	Humans ; SARS-CoV-2 ; Liquid Chromatography-Mass Spectrometry ; Chromatography, Liquid/methods ; COVID-19 ; Tandem Mass Spectrometry/methods ; Phenotype ; Bile Acids and Salts
Chemical Substances	Bile Acids and Salts
Language	English
Publishing date	2024-03-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	2078618-9
ISSN	1535-3907 ; 1535-3893
ISSN (online)	1535-3907
ISSN	1535-3893
DOI	10.1021/acs.jproteome.3c00797
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Article ; Online: Lipidomic features of honey bee and colony health during limited supplementary feeding.

Castaños, Clara E / Boyce, Mary C / Bates, Tiffane / Millar, A Harvey / Flematti, Gavin / Lawler, Nathan G / Grassl, Julia

Insect molecular biology

2023 Volume 32, Issue 6, Page(s) 658–675

Abstract: Honey bee nutritional health depends on nectar and pollen, which provide the main source of carbohydrates, proteins and lipids to individual bees. During malnutrition, insect metabolism accesses fat body reserves. However, this process in bees and its ... ...

Abstract	Honey bee nutritional health depends on nectar and pollen, which provide the main source of carbohydrates, proteins and lipids to individual bees. During malnutrition, insect metabolism accesses fat body reserves. However, this process in bees and its repercussions at the colony level are poorly understood. Using untargeted lipidomics and gene expression analysis, we examined the effects of different feeding treatments (starvation, sugar feeding and sugar + pollen feeding) on bees and correlated them with colony health indicators. We found that nutritional stress led to an increase in unsaturated triacylglycerols and diacylglycerols, as well as a decrease in free fatty acids in the bee fat body. Here, we hypothesise that stored lipids are made available through a process where unsaturations change lipid's structure. Increased gene expression of three lipid desaturases in response to malnutrition supports this hypothesis, as these desaturases may be involved in releasing fatty acyl chains for lipolysis. Although nutritional stress was evident in starving and sugar-fed bees at the colony and physiological level, only starved colonies presented long-term effects in honey production.
MeSH term(s)	Bees ; Animals ; Lipidomics ; Sugars ; Malnutrition ; Fatty Acid Desaturases ; Lipids
Chemical Substances	Sugars ; Fatty Acid Desaturases (EC 1.14.19.-) ; Lipids
Language	English
Publishing date	2023-07-21
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	33602-6
ISSN	1365-2583 ; 0962-1075
ISSN (online)	1365-2583
ISSN	0962-1075
DOI	10.1111/imb.12864
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Article: Metabolomics reveals mouse plasma metabolite responses to acute exercise and effects of disrupting AMPK-glycogen interactions.

Belhaj, Mehdi R / Lawler, Nathan G / Hawley, John A / Broadhurst, David I / Hoffman, Nolan J / Reinke, Stacey N

Frontiers in molecular biosciences

2022 Volume 9, Page(s) 957549

Abstract: Introduction: ...

Abstract	Introduction:
Language	English
Publishing date	2022-08-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2814330-9
ISSN	2296-889X
ISSN	2296-889X
DOI	10.3389/fmolb.2022.957549
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Article ; Online: Retraction: Colossal Density-Driven Resistance Response in the Negative Charge Transfer Insulator MnS_{2} [Phys. Rev. Lett. 127, 016401 (2021)].

Durkee, Dylan / Dasenbrock-Gammon, Nathan / Smith, G Alexander / Snider, Elliot / Smith, Dean / Childs, Christian / Kimber, Simon A J / Lawler, Keith V / Salamat, Ashkan

Physical review letters

2023 Volume 131, Issue 7, Page(s) 79902

Abstract: Retraction of DOI: 10.1103/PhysRevLett.127.016401. ...

Abstract	Retraction of DOI: 10.1103/PhysRevLett.127.016401.
Language	English
Publishing date	2023-09-01
Publishing country	United States
Document type	Retraction of Publication
ZDB-ID	208853-8
ISSN	1079-7114 ; 0031-9007
ISSN (online)	1079-7114
ISSN	0031-9007
DOI	10.1103/PhysRevLett.131.079902
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Article ; Online: Advanced Microsamples

Jayden Lee Roberts / Luke Whiley / Nicola Gray / Melvin Gay / Nathan G. Lawler

Separations, Vol 9, Iss 7, p

Current Applications and Considerations for Mass Spectrometry-Based Metabolic Phenotyping Pipelines

2022 Volume 175

Abstract: Microsamples are collections usually less than 50 µL, although all devices that we have captured as part of this review do not fit within this definition (as some can perform collections of up to 600 µL); however, they are considered microsamples that ... ...

Abstract	Microsamples are collections usually less than 50 µL, although all devices that we have captured as part of this review do not fit within this definition (as some can perform collections of up to 600 µL); however, they are considered microsamples that can be self-administered. These microsamples have been introduced in pre-clinical, clinical, and research settings to overcome obstacles in sampling via traditional venepuncture. However, venepuncture remains the sampling gold standard for the metabolic phenotyping of blood. This presents several challenges in metabolic phenotyping workflows: accessibility for individuals in rural and remote areas (due to the need for trained personnel), the unamenable nature to frequent sampling protocols in longitudinal research (for its invasive nature), and sample collection difficulty in the young and elderly. Furthermore, venous sample stability may be compromised when the temperate conditions necessary for cold-chain transport are beyond control. Alternatively, research utilising microsamples extends phenotyping possibilities to inborn errors of metabolism, therapeutic drug monitoring, nutrition, as well as sport and anti-doping. Although the application of microsamples in metabolic phenotyping exists, it is still in its infancy, with whole blood being overwhelmingly the primary biofluid collected through the collection method of dried blood spots. Research into the metabolic phenotyping of microsamples is limited; however, with advances in commercially available microsampling devices, common barriers such as volumetric inaccuracies and the ‘haematocrit effect’ in dried blood spot microsampling can be overcome. In this review, we provide an overview of the common uses and workflows for microsampling in metabolic phenotyping research. We discuss the advancements in technologies, highlighting key considerations and remaining knowledge gaps for the employment of microsamples in metabolic phenotyping research. This review supports the translation of research from the ‘bench to ...
Keywords	microsampling ; sample miniaturisation ; dried blood spot (DBS) ; dried plasma spot (DPS) ; metabolic phenotyping ; gas chromatography–mass spectrometry (GC–MS) ; Physics ; QC1-999 ; Chemistry ; QD1-999
Subject code	070
Language	English
Publishing date	2022-07-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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Article ; Online: Sensitive and quantitative determination of short-chain fatty acids in human serum using liquid chromatography mass spectrometry.

Shafaei, Armaghan / Vamathevan, Veronica / Pandohee, Jessica / Lawler, Nathan G / Broadhurst, David / Boyce, Mary C

Analytical and bioanalytical chemistry

2021 Volume 413, Issue 25, Page(s) 6333–6342

Abstract: Short-chain fatty acids (SCFAs) are increasingly being monitored to elucidate the link between gut health and disease. These metabolites are routinely measured in faeces, but their determination in serum is more challenging due to their low ... ...

Abstract	Short-chain fatty acids (SCFAs) are increasingly being monitored to elucidate the link between gut health and disease. These metabolites are routinely measured in faeces, but their determination in serum is more challenging due to their low concentrations. A method for the determination of eight SCFAs in serum is described here. High-resolution mass spectrometry and gas chromatography were used to identify the presence of isomeric interferences, which were then overcome through a combination of chromatographic separation and judicious choice of MS fragment ion. The SCFAs were derivatised to form 3-nitrophenylhydrazones before being separated on a reversed-phase column and then detected using liquid chromatography tandem mass spectrometry (LC-QQQ-MS). The LODs and LOQs of SCFAs using this method were in the range 1 to 7 ng mL
MeSH term(s)	Blood Chemical Analysis/methods ; Chromatography, Liquid/methods ; Fatty Acids, Volatile/blood ; Humans ; Mass Spectrometry/methods ; Reproducibility of Results ; Sensitivity and Specificity
Chemical Substances	Fatty Acids, Volatile
Language	English
Publishing date	2021-08-11
Publishing country	Germany
Document type	Journal Article
ZDB-ID	201093-8
ISSN	1618-2650 ; 0016-1152 ; 0372-7920
ISSN (online)	1618-2650
ISSN	0016-1152 ; 0372-7920
DOI	10.1007/s00216-021-03589-w
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Article ; Online: Comprehensive Lipidomic Workflow for Multicohort Population Phenotyping Using Stable Isotope Dilution Targeted Liquid Chromatography-Mass Spectrometry.

Ryan, Monique J / Grant-St James, Alanah / Lawler, Nathan G / Fear, Mark W / Raby, Edward / Wood, Fiona M / Maker, Garth L / Wist, Julien / Holmes, Elaine / Nicholson, Jeremy K / Whiley, Luke / Gray, Nicola

Journal of proteome research

2023 Volume 22, Issue 5, Page(s) 1419–1433

Abstract: Dysregulated lipid metabolism underpins many chronic diseases including cardiometabolic diseases. Mass spectrometry-based lipidomics is an important tool for understanding mechanisms of lipid dysfunction and is widely applied in epidemiology and clinical ...

Abstract	Dysregulated lipid metabolism underpins many chronic diseases including cardiometabolic diseases. Mass spectrometry-based lipidomics is an important tool for understanding mechanisms of lipid dysfunction and is widely applied in epidemiology and clinical studies. With ever-increasing sample numbers, single batch acquisition is often unfeasible, requiring advanced methods that are accurate and robust to batch-to-batch and interday analytical variation. Herein, an optimized comprehensive targeted workflow for plasma and serum lipid quantification is presented, combining stable isotope internal standard dilution, automated sample preparation, and ultrahigh performance liquid chromatography-tandem mass spectrometry with rapid polarity switching to target 1163 lipid species spanning 20 subclasses. The resultant method is robust to common sources of analytical variation including blood collection tubes, hemolysis, freeze-thaw cycles, storage stability, analyte extraction technique, interinstrument variation, and batch-to-batch variation with 820 lipids reporting a relative standard deviation of <30% in 1048 replicate quality control plasma samples acquired across 16 independent batches (total injection count = 6142). However, sample hemolysis of ≥0.4% impacted lipid concentrations, specifically for phosphatidylethanolamines (PEs). Low interinstrument variability across two identical LC-MS systems indicated feasibility for intra/inter-lab parallelization of the assay. In summary, we have optimized a comprehensive lipidomic protocol to support rigorous analysis for large-scale, multibatch applications in precision medicine. The mass spectrometry lipidomics data have been deposited to massIVE: data set identifiers MSV000090952 and 10.25345/C5NP1WQ4S.
MeSH term(s)	Humans ; Lipidomics/methods ; Workflow ; Hemolysis ; Lipids ; Chromatography, Liquid/methods ; Mass Spectrometry/methods
Chemical Substances	Lipids
Language	English
Publishing date	2023-02-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2078618-9
ISSN	1535-3907 ; 1535-3893
ISSN (online)	1535-3907
ISSN	1535-3893
DOI	10.1021/acs.jproteome.2c00682
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