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  1. Article: A review of conventional and sustained-release formulations of oral natural micronized progesterone in obstetric indications.

    Wagh, Girija Narendrakumar / Kundavi Shankar, K M / Bachani, Sumitra

    Drugs in context

    2021  Volume 10

    Abstract: Background: Exogenous progesterone is a treatment option for obstetric indications associated with reduced progesterone activity. Oral natural micronized progesterone (NMP) is effective, although it requires multiple daily doses and may cause adverse ... ...

    Abstract Background: Exogenous progesterone is a treatment option for obstetric indications associated with reduced progesterone activity. Oral natural micronized progesterone (NMP) is effective, although it requires multiple daily doses and may cause adverse events due to its active metabolites. A sustained-release formulation of NMP (NMP-SR) has been developed to overcome the limitations of conventional oral NMP.
    Methods: This narrative review examines the available evidence for oral NMP and NMP-SR in several obstetric indications of interest.
    Results: Literature searches identified 17 studies of oral NMP (luteal phase support during assisted reproduction, prevention of threatened miscarriage, prevention of preterm delivery), and clinical studies supporting use of NMP-SR (luteal phase support during intrauterine insemination, maintenance of high-risk pregnancy). Oral NMP was effective for luteal phase support during
    Conclusions: By releasing progesterone gradually and circumventing first-pass metabolism, NMP-SR elicits the desired therapeutic effect with benefits over conventional oral NMP in terms of bioavailability, once-daily dosing and improved tolerability. Oral NMP-SR appears to be a valuable option for treating obstetric conditions associated with insufficient progesterone exposure.
    Language English
    Publishing date 2021-10-13
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2719560-0
    ISSN 1740-4398 ; 1745-1981
    ISSN (online) 1740-4398
    ISSN 1745-1981
    DOI 10.7573/dic.2021-7-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A comprehensive study of the estuary sea environment in the Bay of Bengal, near the Mahanadi River confluence

    Sharada Shrinivas Pati / Sumitra Nayak / Sangeeta Mishra / Bhabani Shankar Panda / Suravi Susmita Mahala / Sarat Kumar Mohanty / Rajaram Behera / Niranjan Mallick / Kailasam Murugesan

    Discover Water, Vol 3, Iss 1, Pp 1-

    2023  Volume 25

    Abstract: Abstract This study focuses on the environmental conditions of the Mahanadi Estuary near Paradeep Harbor and the adjacent sea. Data collected from May 2013 to April 2020 from 32 GPS fixed stations was analyzed to assess the water quality in different ... ...

    Abstract Abstract This study focuses on the environmental conditions of the Mahanadi Estuary near Paradeep Harbor and the adjacent sea. Data collected from May 2013 to April 2020 from 32 GPS fixed stations was analyzed to assess the water quality in different zones (estuarine, mixed zone, mixed zone south, and mixed zone north) of study area. Parameters such as pH, SST, TSS, nitrite, phosphate, silicate, TOC, chlorophyll, fecal coliform, and heavy metals were used to estimate the Water Quality Index (WQI) for each zone. The study found a deterioration (> 30%) in the overall water quality of the Mahanadi Estuary from 2013 to 2020, potentially attributed to river inflows, port activities, and industrial outflows in to the coastal ecosystem. Seasonal variations in temperature, salinity, turbidity, nitrite, nitrate, and ammonia were observed. The water quality showed a deteriorating trend in estuarine, mixed zone, mixed zone south, and mixed zone north. Based on the water quality indices, the ecosystem shows moderate levels of stress. The degraded water quality highlights the need for a targeted mitigation plan to reduce external pressures and enhance the overall ecosystem quality. Graphical Abstract
    Keywords Mahanadi River ; Bay of Bengal ; Water Quality Index ; Ecological Indicator ; Anthropogenic Pollutant ; Physicochemical factors ; Water supply for domestic and industrial purposes ; TD201-500 ; Environmental sciences ; GE1-350
    Language English
    Publishing date 2023-10-01T00:00:00Z
    Publisher Springer
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article: Genome editing of oncogenes with ZFNs and TALENs: caveats in nuclease design.

    Shankar, Sumitra / Sreekumar, Ahalya / Prasad, Deepti / Das, Ani V / Pillai, M Radhakrishna

    Cancer cell international

    2018  Volume 18, Page(s) 169

    Abstract: Background: Gene knockout technologies involving programmable nucleases have been used to create knockouts in several applications. Gene editing using Zinc-finger nucleases (ZFNs), Transcription activator like effectors (TALEs) and CRISPR/Cas systems ... ...

    Abstract Background: Gene knockout technologies involving programmable nucleases have been used to create knockouts in several applications. Gene editing using Zinc-finger nucleases (ZFNs), Transcription activator like effectors (TALEs) and CRISPR/Cas systems has been used to create changes in the genome in order to make it non-functional. In the present study, we have looked into the possibility of using six fingered CompoZr ZFN pair to target the E6 gene of HPV 16 genome.
    Methods: HPV 16
    Results: Although ZFN editing activity in yeast based MEL-I assay was high, it yielded very low activity in tumor cell lines; only 6% editing in CaSki and negligible activity in SiHa cell lines. Though editing efficiency was better in CaSki, no significant reduction in E6 protein levels was observed in immunocytochemical analysis. Further, in silico analysis of DNA binding prediction revealed that some of the ZFN modules bound to sequence that did not match the target sequence. Hence, alternate ZFN pairs for E6 and E7 were not synthesized since no further active sites could be identified by in silico analyses. Then we designed TALENs to target E6 and E7 gene. TALENs designed to target E7 gene led to reduction of E7 levels in CaSki and SiHa cervical cancer cell lines. However, TALEN designed to target E6 gene did not yield any editing activity.
    Conclusions: Our study highlights that designed nucleases intended to obtain bulk effect should have a reasonable editing activity which reflects phenotypically as well. Nucleases with low editing efficiency, intended for generation of knockout cell lines nucleases could be obtained by single cell cloning. This could serve as a criterion for designing ZFNs and TALENs.
    Language English
    Publishing date 2018-10-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 2091573-1
    ISSN 1475-2867
    ISSN 1475-2867
    DOI 10.1186/s12935-018-0666-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Translating cancer research by synthetic biology.

    Shankar, Sumitra / Pillai, M Radhakrishna

    Molecular bioSystems

    2011  Volume 7, Issue 6, Page(s) 1802–1810

    Abstract: Synthetic biology concerns applying engineering principles to biological systems. Engineering properties such as fine tuning, novel specificity, and modularity could be components of a synthetic toolkit that can be exploited to explore various issues in ... ...

    Abstract Synthetic biology concerns applying engineering principles to biological systems. Engineering properties such as fine tuning, novel specificity, and modularity could be components of a synthetic toolkit that can be exploited to explore various issues in cancer research such as elucidation of mechanisms and pathways, creating new diagnostic tools and novel therapeutic approaches. A repertoire of synthetic biology toolkits involving DNA, RNA and protein bio-parts, have been applied to address the issues of drug target identification, drug discovery and therapeutic treatment in cancer research, thereby projecting a new dimension in oncology research.
    MeSH term(s) Animals ; Antineoplastic Agents/administration & dosage ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents/therapeutic use ; Bioengineering/methods ; Drug Delivery Systems ; Drug Discovery/methods ; Drug Screening Assays, Antitumor/methods ; Humans ; Neoplasms/diagnosis ; Neoplasms/drug therapy ; Neoplasms/pathology ; Protein Engineering/methods ; Synthetic Biology/methods ; Translational Medical Research
    Chemical Substances Antineoplastic Agents
    Language English
    Publishing date 2011-06
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2188635-0
    ISSN 1742-2051 ; 1742-206X
    ISSN (online) 1742-2051
    ISSN 1742-206X
    DOI 10.1039/c1mb05016h
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Stigma and discrimination in individuals with severe and persistent mental illness in an assertive community treatment team: Perceptions of families and healthcare professionals.

    O'Reilly, Claire L / Paul, Diane / McCahon, Rebecca / Shankar, Sumitra / Rosen, Alan / Ramzy, Thomas

    The International journal of social psychiatry

    2019  Volume 65, Issue 7-8, Page(s) 570–579

    Abstract: Aim: To explore family member and staff perceptions of clients' experiences of stigma and discrimination, in those living with severe and persistent mental illness in an Assertive Community Treatment Team.: Method: This qualitative study used the ... ...

    Abstract Aim: To explore family member and staff perceptions of clients' experiences of stigma and discrimination, in those living with severe and persistent mental illness in an Assertive Community Treatment Team.
    Method: This qualitative study used the Discrimination and Stigma Scale to conduct structured face-to-face and telephone interviews of family members and healthcare professionals, working with the Assertive Outreach Team (AOT) (an Assertive Community Treatment Team) of a northern inner suburban catchment of Sydney, New South Wales, Australia.
    Results: Forty-one people participated in the study (23 AOT clinical staff members and 18 family members). Family and clinical staff commonly reported stigma and discrimination amongst their relatives and clients, respectively. Four overarching themes emerged from the data: (1) appearance and behaviour, (2) avoidance and being shunned, (3) key areas of life affected by discrimination and (4) impacts of discrimination and skills to cope with discrimination.
    Conclusion: Reports of stigma and discrimination were common, yet varied between groups with clinical staff commonly witnessing experiences and impacts of discrimination in everyday life, with families' reports being substantially less. Due to the strong advocacy and support provided by the AOT model, clinical staff often buffered experiences of stigma and discrimination. Further research is needed to explore effective interventions to reduce experiences of discrimination in this population group.
    MeSH term(s) Adult ; Community Mental Health Services ; Family ; Female ; Health Personnel ; Humans ; Interviews as Topic ; Male ; Mental Disorders/psychology ; Mental Disorders/therapy ; New South Wales ; Qualitative Research ; Social Discrimination/psychology ; Social Stigma
    Language English
    Publishing date 2019-08-03
    Publishing country England
    Document type Journal Article
    ZDB-ID 3062-4
    ISSN 1741-2854 ; 0020-7640
    ISSN (online) 1741-2854
    ISSN 0020-7640
    DOI 10.1177/0020764019867358
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  6. Article ; Online: TALEN based HPV-E7 editing triggers necrotic cell death in cervical cancer cells

    Sumitra Shankar / Deepti Prasad / Rahul Sanawar / Ani V. Das / M. Radhakrishna Pillai

    Scientific Reports, Vol 7, Iss 1, Pp 1-

    2017  Volume 12

    Abstract: Abstract Human Papillomavirus E7 and E6 oncoproteins have been considered as suitable candidate anti-viral targets since they cause malignant conversion in cervical cancers. Transcription Activator-Like Effector Nucleases (TALENs) are recent editing ... ...

    Abstract Abstract Human Papillomavirus E7 and E6 oncoproteins have been considered as suitable candidate anti-viral targets since they cause malignant conversion in cervical cancers. Transcription Activator-Like Effector Nucleases (TALENs) are recent editing tools to knockout genes by inducing double stranded breaks at specific sites in the genome. In here, we have designed specific TALENs to target E7 and analyzed their efficiency in inducing cell death in cervical cancer cells. We found that designed TALENs could yield about 10–12% editing activity as observed from T7E1 and nuclease resistance assays. Down-regulation of E7 and E6 was further evident at the transcript as well as proteins levels indicating that the selected TALENs were effective. TALEN-mediated E7 editing led to cell death as ascertained by cell cycle and Annexin V assays. Annexin profiling suggested that cell death could be due to necrosis as observed by upregulation of necrotic markers such as LDH A, Rip-1, and Cyclophilin A. Necrosis appears to be a better therapeutic response as it could further activate pro-inflammatory cytokines to attract immune cells to eliminate HPV-integrated cells and therefore TALEN editing strategy has the potential to be a promising tool as an adjuvant therapy in cervical cancer along with surgery.
    Keywords Medicine ; R ; Science ; Q
    Subject code 610
    Language English
    Publishing date 2017-07-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: TALEN based HPV-E7 editing triggers necrotic cell death in cervical cancer cells.

    Shankar, Sumitra / Prasad, Deepti / Sanawar, Rahul / Das, Ani V / Pillai, M Radhakrishna

    Scientific reports

    2017  Volume 7, Issue 1, Page(s) 5500

    Abstract: Human Papillomavirus E7 and E6 oncoproteins have been considered as suitable candidate anti-viral targets since they cause malignant conversion in cervical cancers. Transcription Activator-Like Effector Nucleases (TALENs) are recent editing tools to ... ...

    Abstract Human Papillomavirus E7 and E6 oncoproteins have been considered as suitable candidate anti-viral targets since they cause malignant conversion in cervical cancers. Transcription Activator-Like Effector Nucleases (TALENs) are recent editing tools to knockout genes by inducing double stranded breaks at specific sites in the genome. In here, we have designed specific TALENs to target E7 and analyzed their efficiency in inducing cell death in cervical cancer cells. We found that designed TALENs could yield about 10-12% editing activity as observed from T7E1 and nuclease resistance assays. Down-regulation of E7 and E6 was further evident at the transcript as well as proteins levels indicating that the selected TALENs were effective. TALEN-mediated E7 editing led to cell death as ascertained by cell cycle and Annexin V assays. Annexin profiling suggested that cell death could be due to necrosis as observed by upregulation of necrotic markers such as LDH A, Rip-1, and Cyclophilin A. Necrosis appears to be a better therapeutic response as it could further activate pro-inflammatory cytokines to attract immune cells to eliminate HPV-integrated cells and therefore TALEN editing strategy has the potential to be a promising tool as an adjuvant therapy in cervical cancer along with surgery.
    MeSH term(s) Apoptosis/drug effects ; Apoptosis/genetics ; Cell Line, Tumor ; Female ; Gene Editing ; Gene Expression Regulation, Neoplastic/drug effects ; Genome, Viral ; Humans ; Imidazoles/pharmacology ; Indoles/pharmacology ; Models, Biological ; Necrosis ; Papillomaviridae/genetics ; Papillomavirus E7 Proteins/genetics ; Transcription Activator-Like Effector Nucleases/metabolism ; Uterine Cervical Neoplasms/genetics ; Uterine Cervical Neoplasms/pathology ; Uterine Cervical Neoplasms/virology
    Chemical Substances Imidazoles ; Indoles ; Papillomavirus E7 Proteins ; necrostatin-1 ; Transcription Activator-Like Effector Nucleases (EC 3.1.-)
    Language English
    Publishing date 2017-07-14
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-017-05696-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Stigma and discrimination experienced by people living with severe and persistent mental illness in assertive community treatment settings.

    Ye, Jing / Chen, Timothy F / Paul, Diane / McCahon, Rebecca / Shankar, Sumitra / Rosen, Alan / O'Reilly, Claire L

    The International journal of social psychiatry

    2016  Volume 62, Issue 6, Page(s) 532–541

    Abstract: Aims: To describe the perceived experiences of stigma and discrimination among people living with severe and persistent mental illness in assertive community treatment (ACT teams) settings in New South Wales (NSW), Australia.: Methods: The ... ...

    Abstract Aims: To describe the perceived experiences of stigma and discrimination among people living with severe and persistent mental illness in assertive community treatment (ACT teams) settings in New South Wales (NSW), Australia.
    Methods: The Discrimination and Stigma Scale (DISC) was used in this cross-sectional study with people living with severe and persistent mental illness. The DISC is a reliable and valid, quantitative and qualitative instrument used to explore and measure levels of negative, anticipated and positive discrimination. Relevant clinical history and socio-demographic information were also collected.
    Results: A total of 50 clients participated, with 40 (80%) reporting experienced negative discrimination in at least one life area. Negative discrimination was most commonly experienced in being avoided or shunned (n=25, 50%), by neighbours (n=24, 48%) and family (n=23, 46%). Anticipated discrimination was common, with half of participants (n=25, 50%) feeling the need to conceal their mental health diagnosis.
    Conclusion: Discrimination was highly prevalent in everyday aspects of life. While healthcare professionals often tend to increase perceived stigma and discrimination, this was only experienced in interactions with general health professionals, while interactions with ACT team members decreased perceived stigma and increased positive discrimination. This indicates that healthcare professionals potentially have a significant role in reducing stigma and discrimination in mental health and that such an effect may be optimised in an ACT team setting.
    MeSH term(s) Adult ; Community Mental Health Services ; Cross-Sectional Studies ; Female ; Health Personnel ; Humans ; Interviews as Topic ; Male ; Mental Disorders/psychology ; Mental Disorders/therapy ; Mental Health ; Middle Aged ; New South Wales ; Social Discrimination/statistics & numerical data ; Social Stigma
    Language English
    Publishing date 2016-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 3062-4
    ISSN 1741-2854 ; 0020-7640
    ISSN (online) 1741-2854
    ISSN 0020-7640
    DOI 10.1177/0020764016651459
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 181: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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