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  1. Article ; Online: Using Adherence and Competence Measures Based on Practice Elements to Evaluate Treatment Fidelity for Two CBT Programs for Youth Anxiety.

    Violante, Stephanie / McLeod, Bryce D / Southam-Gerow, Michael A / Chorpita, Bruce F / Weisz, John R

    Behavior therapy

    2023  Volume 55, Issue 3, Page(s) 605–620

    Abstract: ... the CBAY Competence Scale (CBAY-C), can assess the quantity (the degree to which prescribed therapeutic ... 796) from 55 youth participants (M age = 9.89 years, SD = 1.71; 46% female; 55% White) with primary ... training program), and (c) usual clinical care. Interrater reliability for the CBAY-A items was good across ...

    Abstract Measures designed to assess the quantity and quality of practices found across treatment programs for specific youth emotional or behavioral problems may be a good fit for evaluating treatment fidelity in effectiveness and implementation research. Treatment fidelity measures must demonstrate certain reliability and validity characteristics to realize this potential. This study examines the extent to which two observational measures, the Cognitive-Behavioral Treatment for Anxiety in Youth Adherence Scale (CBAY-A) and the CBAY Competence Scale (CBAY-C), can assess the quantity (the degree to which prescribed therapeutic techniques are delivered as intended) or quality (the competence with which prescribed techniques are delivered) of practices found in two distinct treatment programs for youth anxiety. Treatment sessions (N = 796) from 55 youth participants (M age = 9.89 years, SD = 1.71; 46% female; 55% White) with primary anxiety problems who participated in an effectiveness study were independently coded by raters who coded quantity, quality, and the youth-clinician alliance. Youth received one of three treatments: (a) standard (i.e., cognitive-behavioral therapy program), (b) modular (i.e., a cognitive-behavioral and parent-training program), and (c) usual clinical care. Interrater reliability for the CBAY-A items was good across the standard and modular conditions but mixed for the CBAY-C items. Across the standard and modular conditions, the CBAY-A Model subscale scores demonstrated evidence of construct validity, but the CBAY-C Model subscale scores showed mixed evidence. The results provide preliminary evidence that the CBAY-A can be used across different treatment programs but raise concerns about the generalizability of the CBAY-C.
    MeSH term(s) Humans ; Cognitive Behavioral Therapy/methods ; Female ; Male ; Child ; Anxiety Disorders/therapy ; Reproducibility of Results ; Adolescent ; Anxiety/therapy ; Anxiety/psychology ; Patient Compliance/statistics & numerical data
    Language English
    Publishing date 2023-09-26
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 211996-1
    ISSN 1878-1888 ; 0005-7894
    ISSN (online) 1878-1888
    ISSN 0005-7894
    DOI 10.1016/j.beth.2023.09.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Antibiotic activity of extract of western red cedar heartwood.

    SOUTHAM, C M

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)

    2010  Volume 61, Page(s) 391–396

    MeSH term(s) Anti-Bacterial Agents ; Antibiotics, Antitubercular ; Dermatologic Agents ; Humans ; Thuja ; Wood
    Chemical Substances Anti-Bacterial Agents ; Antibiotics, Antitubercular ; Dermatologic Agents
    Language English
    Publishing date 2010-10-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 4015-0
    ISSN 1535-3699 ; 1525-1373 ; 0037-9727
    ISSN (online) 1535-3699 ; 1525-1373
    ISSN 0037-9727
    DOI 10.3181/00379727-61-15330
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Primary osteoarthritis chondrocyte map of chromatin conformation reveals novel candidate effector genes.

    Bittner, Norbert / Shi, Chenfu / Zhao, Danyun / Ding, James / Southam, Lorraine / Swift, Diane / Kreitmaier, Peter / Tutino, Mauro / Stergiou, Odysseas / Cheung, Jackson T S / Katsoula, Georgia / Hankinson, Jenny / Wilkinson, Jeremy Mark / Orozco, Gisela / Zeggini, Eleftheria

    Annals of the rheumatic diseases

    2024  

    Abstract: ... genome chromosome conformation analysis (Hi-C) map of primary osteoarthritis chondrocytes and identify ... patients with knee osteoarthritis underwent Hi-C analysis to link chromosomal structure to genomic sequence ... We have constructed the first Hi-C map of primary human chondrocytes and have made it available ...

    Abstract Objectives: Osteoarthritis is a complex disease with a huge public health burden. Genome-wide association studies (GWAS) have identified hundreds of osteoarthritis-associated sequence variants, but the effector genes underpinning these signals remain largely elusive. Understanding chromosome organisation in three-dimensional (3D) space is essential for identifying long-range contacts between distant genomic features (e.g., between genes and regulatory elements), in a tissue-specific manner. Here, we generate the first whole genome chromosome conformation analysis (Hi-C) map of primary osteoarthritis chondrocytes and identify novel candidate effector genes for the disease.
    Methods: Primary chondrocytes collected from 8 patients with knee osteoarthritis underwent Hi-C analysis to link chromosomal structure to genomic sequence. The identified loops were then combined with osteoarthritis GWAS results and epigenomic data from primary knee osteoarthritis chondrocytes to identify variants involved in gene regulation via enhancer-promoter interactions.
    Results: We identified 345 genetic variants residing within chromatin loop anchors that are associated with 77 osteoarthritis GWAS signals. Ten of these variants reside directly in enhancer regions of 10 newly described active enhancer-promoter loops, identified with multiomics analysis of publicly available chromatin immunoprecipitation sequencing (ChIP-seq) and assay for transposase-accessible chromatin using sequencing (ATAC-seq) data from primary knee chondrocyte cells, pointing to two new candidate effector genes
    Conclusions: We have constructed the first Hi-C map of primary human chondrocytes and have made it available as a resource for the scientific community. By integrating 3D genomics with large-scale genetic association and epigenetic data, we identify novel candidate effector genes for osteoarthritis, which enhance our understanding of disease and can serve as putative high-value novel drug targets.
    Language English
    Publishing date 2024-03-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 7090-7
    ISSN 1468-2060 ; 0003-4967
    ISSN (online) 1468-2060
    ISSN 0003-4967
    DOI 10.1136/ard-2023-224945
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Assessing Stakeholder Perceptions of the Utility of Genetic Information for the Clinical Care of Mental Health Disorders: We Have a Will but Need to See the Way.

    Bourdon, Jessica L / Hettema, John M / Prom-Wormley, Elizabeth C / Southam-Gerow, Michael A

    Administration and policy in mental health

    2020  Volume 48, Issue 2, Page(s) 363–376

    Abstract: Academic stakeholders' (primarily mental health researchers and clinicians) practices and attitudes related to the translation of genetic information into mental health care were assessed. A three-part survey was administered at two large, urban ... ...

    Abstract Academic stakeholders' (primarily mental health researchers and clinicians) practices and attitudes related to the translation of genetic information into mental health care were assessed. A three-part survey was administered at two large, urban universities. Response frequencies were calculated. Participants (N = 64) reported moderate levels of translational practice, adequate levels of genetic knowledge, and variable levels of genetic competence. They held positive attitudes toward translating genetic information about mental health broadly but negative attitudes about the impact that such information would have on specific aspects of care. The current study lays the groundwork for further inquiry into translating genetic information to mental health care.
    MeSH term(s) Health Knowledge, Attitudes, Practice ; Humans ; Mental Disorders/genetics ; Mental Disorders/therapy ; Mental Health ; Perception ; Surveys and Questionnaires
    Language English
    Publishing date 2020-06-19
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 1025319-1
    ISSN 1573-3289 ; 0894-587X
    ISSN (online) 1573-3289
    ISSN 0894-587X
    DOI 10.1007/s10488-020-01058-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: CORM-3 induces DNA damage through Ru(II) binding to DNA.

    Lyon, Rhiannon F / Southam, Hannah M / Trevitt, Clare R / Liao, Chunyan / El-Khamisy, Sherif F / Poole, Robert K / Williamson, Mike P

    The Biochemical journal

    2022  Volume 479, Issue 13, Page(s) 1429–1439

    Abstract: When the 'CO-releasing molecule-3', CORM-3 (Ru(CO)3Cl(glycinate)), is dissolved in water it forms a range of ruthenium complexes. These are taken up by cells and bind to intracellular ligands, notably thiols such as cysteine and glutathione, where the Ru( ...

    Abstract When the 'CO-releasing molecule-3', CORM-3 (Ru(CO)3Cl(glycinate)), is dissolved in water it forms a range of ruthenium complexes. These are taken up by cells and bind to intracellular ligands, notably thiols such as cysteine and glutathione, where the Ru(II) reaches high intracellular concentrations. Here, we show that the Ru(II) ion also binds to DNA, at exposed guanosine N7 positions. It therefore has a similar cellular target to the anticancer drug cisplatin, but not identical, because Ru(II) shows no evidence of forming intramolecular crossbridges in the DNA. The reaction is slow, and with excess Ru, intermolecular DNA crossbridges are formed. The addition of CORM-3 to human colorectal cancer cells leads to strand breaks in the DNA, as assessed by the alkaline comet assay. DNA damage is inhibited by growth media containing amino acids, which bind to extracellular Ru and prevent its entry into cells. We conclude that the cytotoxicity of Ru(II) is different from that of platinum, making it a promising development target for cancer therapeutics.
    MeSH term(s) Antineoplastic Agents/chemistry ; DNA ; DNA Damage ; Humans ; Neoplasms ; Ruthenium/chemistry ; Ruthenium/metabolism ; Ruthenium/pharmacology
    Chemical Substances Antineoplastic Agents ; Ruthenium (7UI0TKC3U5) ; DNA (9007-49-2)
    Language English
    Publishing date 2022-06-21
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2969-5
    ISSN 1470-8728 ; 0006-2936 ; 0306-3275 ; 0264-6021
    ISSN (online) 1470-8728
    ISSN 0006-2936 ; 0306-3275 ; 0264-6021
    DOI 10.1042/BCJ20220254
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: A 3-mirror surface force balance for the investigation of fluids confined to nanoscale films between two ultra-smooth polarizable electrodes.

    van Engers, C D / Balabajew, M / Southam, A / Perkin, S

    The Review of scientific instruments

    2018  Volume 89, Issue 12, Page(s) 123901

    Abstract: We present a new technique, based on the Surface Force Balance (SFB), for the direct measurement of surface forces between two ultra-smooth and polarizable gold electrode surfaces across thin fluid films. Combining the direct interferometric measurement ... ...

    Abstract We present a new technique, based on the Surface Force Balance (SFB), for the direct measurement of surface forces between two ultra-smooth and polarizable gold electrode surfaces across thin fluid films. Combining the direct interferometric measurement of surface separation and contact geometry with smooth electrode surfaces has proved challenging in the past, and for this reason, previous measurements with the SFB typically involved two insulating mica surfaces, or one mica surface and one electrode surface, or an alternative less direct measure of the surface separation. Here, we demonstrate that a 3-mirror interferometer can overcome these difficulties: the setup involves two ultra-smooth electrode/mirror surfaces between which the fluid is confined and a third mirror to allow for interferometric detection of the liquid thickness with nanometer resolution and at thicknesses much smaller than the diffraction limit of the light. We conclude with a proof-of-concept measurement across dry nitrogen gas. The technique should prove useful for studying the properties of fluids confined at the nanoscale inside a slit-pore of controlled electrical potential or subject to applied electric fields.
    Language English
    Publishing date 2018-10-24
    Publishing country United States
    Document type Journal Article
    ZDB-ID 209865-9
    ISSN 1089-7623 ; 0034-6748
    ISSN (online) 1089-7623
    ISSN 0034-6748
    DOI 10.1063/1.5045485
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  7. Article: Intermittent Surface Oxygenation Results in Similar Mitochondrial Protection and Maintenance of Aerobic Metabolism as Compared to Continuous Oxygenation during Hypothermic Machine Kidney Machine Perfusion.

    Darius, Tom / Vergauwen, Martial / Maistriaux, Louis / Evrard, Robin / Schlegel, Andrea / Mueller, Matteo / O'Neil, Donna / Southam, Andrew / Aydin, Selda / Devresse, Arnaud / De Meyer, Martine / Gianello, Pierre / Ludwig, Christian / Dutkowski, Philipp / Mourad, Michel

    Journal of clinical medicine

    2023  Volume 12, Issue 11

    Abstract: Short bubble and subsequent surface oxygenation is an innovative oxygenation technique and alternative for membrane oxygenation during hypothermic machine perfusion (HMP). The metabolic effect of the interruption of surface oxygenation for 4 h (mimicking ...

    Abstract Short bubble and subsequent surface oxygenation is an innovative oxygenation technique and alternative for membrane oxygenation during hypothermic machine perfusion (HMP). The metabolic effect of the interruption of surface oxygenation for 4 h (mimicking organ transport) during HMP was compared to continuous surface and membrane oxygenation in a pig kidney ex situ preservation model. After 30 min of warm ischemia by vascular clamping, a kidney of a ±40 kg pig was procured and subsequently preserved according to one of the following groups: (1) 22-h HMP + intermittent surface oxygenation (
    Language English
    Publishing date 2023-05-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm12113731
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Demonstrating the reliability of in vivo metabolomics based chemical grouping: towards best practice.

    Viant, Mark R / Amstalden, E / Athersuch, T / Bouhifd, M / Camuzeaux, S / Crizer, D M / Driemert, P / Ebbels, T / Ekman, D / Flick, B / Giri, V / Gómez-Romero, M / Haake, V / Herold, M / Kende, A / Lai, F / Leonards, P E G / Lim, P P / Lloyd, G R /
    Mosley, J / Namini, C / Rice, J R / Romano, S / Sands, C / Smith, M J / Sobanski, T / Southam, A D / Swindale, L / van Ravenzwaay, B / Walk, T / Weber, R J M / Zickgraf, F M / Kamp, H

    Archives of toxicology

    2024  Volume 98, Issue 4, Page(s) 1111–1123

    Abstract: While grouping/read-across is widely used to fill data gaps, chemical registration dossiers are often rejected due to weak category justifications based on structural similarity only. Metabolomics provides a route to robust chemical categories via ... ...

    Abstract While grouping/read-across is widely used to fill data gaps, chemical registration dossiers are often rejected due to weak category justifications based on structural similarity only. Metabolomics provides a route to robust chemical categories via evidence of shared molecular effects across source and target substances. To gain international acceptance, this approach must demonstrate high reliability, and best-practice guidance is required. The MetAbolomics ring Trial for CHemical groupING (MATCHING), comprising six industrial, government and academic ring-trial partners, evaluated inter-laboratory reproducibility and worked towards best-practice. An independent team selected eight substances (WY-14643, 4-chloro-3-nitroaniline, 17α-methyl-testosterone, trenbolone, aniline, dichlorprop-p, 2-chloroaniline, fenofibrate); ring-trial partners were blinded to their identities and modes-of-action. Plasma samples were derived from 28-day rat tests (two doses per substance), aliquoted, and distributed to partners. Each partner applied their preferred liquid chromatography-mass spectrometry (LC-MS) metabolomics workflows to acquire, process, quality assess, statistically analyze and report their grouping results to the European Chemicals Agency, to ensure the blinding conditions of the ring trial. Five of six partners, whose metabolomics datasets passed quality control, correctly identified the grouping of eight test substances into three categories, for both male and female rats. Strikingly, this was achieved even though a range of metabolomics approaches were used. Through assessing intrastudy quality-control samples, the sixth partner observed high technical variation and was unable to group the substances. By comparing workflows, we conclude that some heterogeneity in metabolomics methods is not detrimental to consistent grouping, and that assessing data quality prior to grouping is essential. We recommend development of international guidance for quality-control acceptance criteria. This study demonstrates the reliability of metabolomics for chemical grouping and works towards best-practice.
    MeSH term(s) Rats ; Male ; Female ; Animals ; Reproducibility of Results ; Metabolomics/methods ; Liquid Chromatography-Mass Spectrometry ; Workflow
    Language English
    Publishing date 2024-02-18
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 124992-7
    ISSN 1432-0738 ; 0340-5761
    ISSN (online) 1432-0738
    ISSN 0340-5761
    DOI 10.1007/s00204-024-03680-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: The acceptability of the Fire and Rescue Service working with primary care to improve identification of mental health problems in older adults. A mixed-method qualitative study.

    Fisher, Tamsin / Chew-Graham, Carolyn A / Corp, Nadia / Farooq, Saeed / Kingston, Paul / Read, Ian / Southam, Jane / Spolander, Gary / Stevens, Dean / Walchester, Mark / Warren, Carmel / Kingstone, Tom

    BJGP open

    2023  Volume 7, Issue 4

    Abstract: Background: Mental ill-health in older adults (aged 60 years and over) is often underdiagnosed and undertreated. Older adults are less likely to access mental health services owing to perceived stigma and fear of being a burden. Non-traditional ... ...

    Abstract Background: Mental ill-health in older adults (aged 60 years and over) is often underdiagnosed and undertreated. Older adults are less likely to access mental health services owing to perceived stigma and fear of being a burden. Non-traditional providers of health care, such as the Fire and Rescue Services (FRS), provide a possible solution to facilitate early detection of problems and help-seeking among older adults, especially in the context of pressured statutory services.
    Aim: To examine whether and how FRS Home Fire Safety Visits (HFSV) could be optimised to include detection and signposting for mental health problems - particularly anxiety and depression - in older adults.
    Design & setting: This mixed-method qualitative study took place in the West Midlands, UK in 2022.
    Method: This study involved focus groups (
    Results: FRS staff were open to expanding their HFSVs to include mental health, provided they had sufficient training and support from partner agencies in primary and social care settings to accept referrals for service users presenting with symptoms of anxiety and/or depression.
    Conclusion: The positive reputation of FRS staff and engagement with older adults suggests that HFSV could support the detection of anxiety and depression in older adults, and appropriate signposting to other services including primary care.
    Language English
    Publishing date 2023-12-19
    Publishing country England
    Document type Journal Article
    ISSN 2398-3795
    ISSN (online) 2398-3795
    DOI 10.3399/BJGPO.2023.0059
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  10. Article ; Online: Characterization of Monophasic Solvent-Based Tissue Extractions for the Detection of Polar Metabolites and Lipids Applying Ultrahigh-Performance Liquid Chromatography-Mass Spectrometry Clinical Metabolic Phenotyping Assays.

    Southam, Andrew D / Pursell, Harriet / Frigerio, Gianfranco / Jankevics, Andris / Weber, Ralf J M / Dunn, Warwick B

    Journal of proteome research

    2020  Volume 20, Issue 1, Page(s) 831–840

    Abstract: ... and nonpolar extracts by C ...

    Abstract Metabolic phenotyping of tissues uses metabolomics and lipidomics to measure the relative polar and nonpolar (lipid) metabolite levels in biological samples. This approach aims to understand disease biochemistry and identify biochemical markers of disease. Sample preparation methods must be reproducible, sensitive (high metabolite and lipid yield), and ideally rapid. We evaluated three biphasic methods for polar and nonpolar compound extraction (chloroform/methanol/water, dichloromethane/methanol/water, and methyl tert-butyl ether [MTBE]/methanol/water), a monophasic method for polar compound extraction (acetonitrile/methanol/water), and a monophasic method for nonpolar compound extraction (isopropanol/water). All methods were applied to mammalian heart, kidney, and liver tissues. Polar extracts were analyzed by hydrophilic interaction chromatography (HILIC) ultrahigh-performance liquid chromatography-mass spectrometry (UHPLC-MS) and nonpolar extracts by C
    MeSH term(s) Animals ; Chromatography, High Pressure Liquid ; Chromatography, Liquid ; Lipids ; Reproducibility of Results ; Solvents ; Tandem Mass Spectrometry
    Chemical Substances Lipids ; Solvents
    Language English
    Publishing date 2020-11-25
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2078618-9
    ISSN 1535-3907 ; 1535-3893
    ISSN (online) 1535-3907
    ISSN 1535-3893
    DOI 10.1021/acs.jproteome.0c00660
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