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Article: Phosphatidylinositol 4,5-Bisphosphate and Cholesterol Regulators of the Calcium-Activated Chloride Channels TMEM16A and TMEM16B.

Arreola, Jorge / López-Romero, Ana Elena / Pérez-Cornejo, Patricia / Rodríguez-Menchaca, Aldo A

Advances in experimental medicine and biology

2023 Volume 1422, Page(s) 279–304

Abstract: Chloride fluxes through homo-dimeric calcium-activated channels TMEM16A and TMEM16B are critical to blood pressure, gastrointestinal motility, hormone, fluid and electrolyte secretion, pain sensation, sensory transduction, and neuronal and muscle ... ...

Abstract	Chloride fluxes through homo-dimeric calcium-activated channels TMEM16A and TMEM16B are critical to blood pressure, gastrointestinal motility, hormone, fluid and electrolyte secretion, pain sensation, sensory transduction, and neuronal and muscle excitability. Their gating depends on the voltage-dependent binding of two intracellular calcium ions to a high-affinity site formed by acidic residues from α-helices 6-8 in each monomer. Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), a low-abundant lipid of the inner leaflet, supports TMEM16A function; it allows TMEM16A to evade the down-regulation induced by calcium, poly-L-lysine, or PI(4,5)P2 5-phosphatase. In stark contrast, adding or removing PI(4,5)P2 diminishes or increases TMEM16B function, respectively. PI(4,5)P2-binding sites on TMEM16A, and presumably on TMEM16B, are on the cytosolic side of α-helices 3-5, opposite the calcium-binding sites. This modular structure suggested that PI(4,5)P2 and calcium cooperate to maintain the conductive state in TMEM16A. Cholesterol, the second-largest constituent of the plasma membrane, also regulates TMEM16A though the mechanism, functional outcomes, binding site(s), and effects on TMEM16A and TMEM16B remain unknown.
MeSH term(s)	Humans ; Chloride Channels/genetics ; Chloride Channels/chemistry ; Chloride Channels/metabolism ; Anoctamin-1/metabolism ; Phosphatidylinositols ; Calcium/metabolism ; Cholesterol ; Calcium Channels ; HEK293 Cells
Chemical Substances	Chloride Channels ; Anoctamin-1 ; Phosphatidylinositols ; Calcium (SY7Q814VUP) ; Cholesterol (97C5T2UQ7J) ; Calcium Channels
Language	English
Publishing date	2023-03-29
Publishing country	United States
Document type	Journal Article
ISSN	2214-8019 ; 0065-2598
ISSN (online)	2214-8019
ISSN	0065-2598
DOI	10.1007/978-3-031-21547-6_10
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Article ; Online: TRP ion channels: Proteins with conformational flexibility.

López-Romero, Ana Elena / Hernández-Araiza, Ileana / Torres-Quiroz, Francisco / Tovar-Y-Romo, Luis B / Islas, León D / Rosenbaum, Tamara

Channels (Austin, Tex.)

2019 Volume 13, Issue 1, Page(s) 207–226

Abstract: Ion channels display conformational changes in response to binding of their agonists and antagonists. The study of the relationships between the structure and the function of these proteins has witnessed considerable advances in the last two decades ... ...

Abstract	Ion channels display conformational changes in response to binding of their agonists and antagonists. The study of the relationships between the structure and the function of these proteins has witnessed considerable advances in the last two decades using a combination of techniques, which include electrophysiology, optical approaches (i.e. patch clamp fluorometry, incorporation of non-canonic amino acids, etc.), molecular biology (mutations in different regions of ion channels to determine their role in function) and those that have permitted the resolution of their structures in detail (X-ray crystallography and cryo-electron microscopy). The possibility of making correlations among structural components and functional traits in ion channels has allowed for more refined conclusions on how these proteins work at the molecular level. With the cloning and description of the family of Transient Receptor Potential (TRP) channels, our understanding of several sensory-related processes has also greatly moved forward. The response of these proteins to several agonists, their regulation by signaling pathways as well as by protein-protein and lipid-protein interactions and, in some cases, their biophysical characteristics have been studied thoroughly and, recently, with the resolution of their structures, the field has experienced a new boom. This review article focuses on the conformational changes in the pores, concentrating on some members of the TRP family of ion channels (TRPV and TRPA subfamilies) that result in changes in their single-channel conductances, a phenomenon that may lead to fine-tuning the electrical response to a given agonist in a cell.
MeSH term(s)	Animals ; Humans ; Multigene Family ; Protein Conformation ; Signal Transduction ; Transient Receptor Potential Channels/chemistry ; Transient Receptor Potential Channels/genetics ; Transient Receptor Potential Channels/metabolism
Chemical Substances	Transient Receptor Potential Channels
Language	English
Publishing date	2019-06-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2262854-X
ISSN	1933-6969 ; 1933-6950
ISSN (online)	1933-6969
ISSN	1933-6950
DOI	10.1080/19336950.2019.1626793
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Article: Physical Activity in Work and Leisure Time during Pregnancy, and Its Influence on Maternal Health and Perinatal Outcomes.

González-Cazorla, Ernesto / Brenes-Romero, Ana Pilar / Sánchez-Gómez, María José / Estévez-Ruiz, Elena / Díaz-Enjuto, Antonio / Cantón-Cisneros, Ana / Lubián-López, Daniel / Mozas-Moreno, Juan / González-Mesa, Ernesto S

Journal of clinical medicine

2024 Volume 13, Issue 3

Abstract: Background: Physical inactivity during pregnancy has been shown to be linked to an increased risk of complications. However, during pregnancy, doubts arise about what type, intensity and frequency of physical activity are most recommended.: Objective!# ...

Abstract	Background: Physical inactivity during pregnancy has been shown to be linked to an increased risk of complications. However, during pregnancy, doubts arise about what type, intensity and frequency of physical activity are most recommended. Objective: Our main objective was to know the level of physical activity (PA) and sedentary lifestyle in a representative sample of pregnant women in Málaga, one of the most populated cities in Spain. Also, we aimed to find out the effects of PA on obstetric and perinatal outcomes and on the mental health of pregnant women, differentiated according to PA intensity and domain. Methods: Five hundred and forty full-term pregnant women who had their obstetric checks in the maternity ward of the Regional University Hospital of Málaga were recruited through consecutive sampling. Participants answered a questionnaire that included the WHO Global Physical Activity Questionnaire (GPAQ), the Edinburgh Depression Scale (EDS), the Generalized Anxiety Disorder Scale (GAD-7) and some other sociodemographic and health-related questions. Subsequently, information about perinatal outcomes was obtained after birth. Results: Only 50.8% of women followed the WHO recommendations on activity. We found a high proportion of obese pregnant women and a direct effect of a sedentary lifestyle on the rate of cesarean sections and vulvovaginal tears in spontaneous births, as well as on the mental health of future mothers. Women's age, the number of children, BMI at the beginning of pregnancy and leisure time physical activity (LTPA) explained anxiety scores, and age, LTPA, BMI at the end of pregnancy and intense work-related physical activity (WTPA) predicted depression scores. Conclusions: LTPA improves obstetric outcomes, helping to reduce the rate of cesarean sections and vulvovaginal tears, as well as reducing prenatal anxiety and depression.
Language	English
Publishing date	2024-01-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662592-1
ISSN	2077-0383
ISSN	2077-0383
DOI	10.3390/jcm13030723
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Article ; Online: Impact of Multiple Sclerosis on Foot Health and Quality of Life: A Prospective Case-Control Investigation.

Ruiz-Sánchez, Francisco Javier / Martins, Maria do Rosário / Losa-Iglesias, Marta Elena / Becerro-de-Bengoa-Vallejo, Ricardo / Gómez-Salgado, Juan / Romero-Morales, Carlos / Jiménez-Cebrián, Ana María / López-López, Daniel

International journal of public health

2023 Volume 68, Page(s) 1605593

Abstract: Objectives: ...

Abstract	Objectives:
MeSH term(s)	Male ; Humans ; Female ; Quality of Life ; Multiple Sclerosis ; Health Status ; Pain ; Case-Control Studies
Language	English
Publishing date	2023-02-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2274130-6
ISSN	1661-8564 ; 1661-8556
ISSN (online)	1661-8564
ISSN	1661-8556
DOI	10.3389/ijph.2023.1605593
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Article ; Online: A novel biocompatible polymer derived from D-mannitol used as a vector in the field of genetic engineering of eukaryotic cells.

Pérez, David / Moyá, María Luisa / Bautista, María / León, Rosa / Molina-Márquez, Ana / Vila, Marta / Romero-Azogil, Lucía / Benito, Elena / de Gracia García-Martín, María / Moreno-Gordillo, Paula / Rosado, Iván V / Balestra, Fernando R / Huertas, Pablo / López-López, Manuel / López-Cornejo, Pilar

Colloids and surfaces. B, Biointerfaces

2023 Volume 224, Page(s) 113219

Abstract: The design and preparation of new vectors to transport genetic material and increase the transfection efficiency continue being an important research line. Here, a novel biocompatible sugar-based polymer derived from D-mannitol has been synthesized to be ...

Abstract	The design and preparation of new vectors to transport genetic material and increase the transfection efficiency continue being an important research line. Here, a novel biocompatible sugar-based polymer derived from D-mannitol has been synthesized to be used as a gene material nanocarrier in human (gene transfection) and microalga cells (transformation process). Its low toxicity allows its use in processes with both medical and industrial applications. A multidisciplinary study about the formation of polymer/p-DNA polyplexes has been carried out using techniques such as gel electrophoresis, zeta potential, dynamic light scattering, atomic force microscopy, and circular dichroism spectroscopy. The nucleic acids used were the eukaryotic expression plasmid pEGFP-C1 and the microalgal expression plasmid Phyco69, which showed different behaviors. The importance of DNA supercoiling in both transfection and transformation processes was demonstrated. Better results were obtained in microalga cells nuclear transformation than in human cells gene transfection. This was related to the plasmid's conformational changes, in particular to their superhelical structure. It is noteworthy that the same nanocarrier has been used with eukaryotic cells from both human and microalga.
MeSH term(s)	Humans ; Polymers/chemistry ; Eukaryotic Cells ; Mannitol ; Transfection ; Plasmids/genetics ; DNA/chemistry ; Genetic Engineering ; Genetic Vectors/genetics
Chemical Substances	Polymers ; Mannitol (3OWL53L36A) ; DNA (9007-49-2)
Language	English
Publishing date	2023-02-23
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500523-9
ISSN	1873-4367 ; 0927-7765
ISSN (online)	1873-4367
ISSN	0927-7765
DOI	10.1016/j.colsurfb.2023.113219
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Article: Reliability of Frail and Barthel Tests for Detecting Frailty in Palliative Oncological Patients in a Home Hospitalization Unit: A Comparative Study.

León-Ramón, Susana / Navarro-Flores, Emmanuel / Losa-Iglesias, Marta Elena / Becerro-de-Bengoa-Vallejo, Ricardo / Jiménez-Cebrián, Ana María / Romero-Morales, Carlos / Palomo-López, Patricia / López-López, Daniel

Life (Basel, Switzerland)

2022 Volume 12, Issue 2

Abstract: Cancer is a condition that can increase the risk of frailty. In addition, palliative oncological patients in home hospitalization can find their activities of daily living affected. The main objective was to measure the degree of frailty in the ... ...

Abstract	Cancer is a condition that can increase the risk of frailty. In addition, palliative oncological patients in home hospitalization can find their activities of daily living affected. The main objective was to measure the degree of frailty in the oncological population in home hospitalization comparing Barthel and Frail-VIG Indexes. This is a descriptive cross-sectional study. A sample of oncological patients in home hospitalization (
Language	English
Publishing date	2022-02-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662250-6
ISSN	2075-1729
ISSN	2075-1729
DOI	10.3390/life12020286
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Article ; Online: The implications of the foot health status in Parkinson patients: A case-control study.

Jiménez-Cebrián, Ana María / López-López, Luis / Losa-Iglesias, Marta Elena / Becerro-de-Bengoa-Vallejo, Ricardo / Romero-Morales, Carlos / López-López, Daniel / Montiel-Luque, Alonso / Navarro-Flores, Emmanuel / de Labra, Carmen

International wound journal

2022

Abstract: Parkinson's disease (PD) is a neurodegenerative disorder that affects both health of the feet, as to gait patterns. This study aimed to find out about foot problems and their impact on self-perceived quality of life and related to foot health in ... ...

Abstract	Parkinson's disease (PD) is a neurodegenerative disorder that affects both health of the feet, as to gait patterns. This study aimed to find out about foot problems and their impact on self-perceived quality of life and related to foot health in Parkinson's patients compared to a group of healthy subjects and to measure it with Spanish Podiatry Health Questionnaire (PHQ-S). It is about a case-control study in a sample of Parkinson's patients n = 62, healthy controls n = 62. The PHQ-S was reported, it describes perception the subject has in each of podiatric 6 dimensions consulted, assessing appreciation of health status of interviewee's feet and a self-rated the foot health score on the visual analog scale (VAS). There were statistically significant differences (P < 0.05) in the dimensions that assessed problems with walking and moving, nail trimming, concern feet state, and affectation of quality of life related foot health. Regarding the self-perception of state of their feet, Parkinson's patients perceive a worse state of health of their feet than healthy subjects. The mean value was 4.8 (SD 2.2) for Parkinson's patients and 3.8 (SD 2.3) for healthy subjects. In conclusion, patients with PD have problems in walking or moving, foot pain, difficulties in foot hygiene and in cutting for their nails, as well as the concern they suffer from deterioration in state of their feet affect them and decrease their quality of life. Podiatric problems in Parkinson's patients have a great impact in reducing quality of life related to foot health.
Language	English
Publishing date	2022-05-17
Publishing country	England
Document type	Journal Article
ZDB-ID	2170920-8
ISSN	1742-481X ; 1742-4801
ISSN (online)	1742-481X
ISSN	1742-4801
DOI	10.1111/iwj.13844
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Article: Repeatability and reliability of the footwear assessment tool in Spanish patients: A transcultural adaptation.

Navarro-Flores, Emmanuel / Losa-Iglesias, Marta Elena / Casado-Hernández, Israel / Becerro-de-Bengoa-Vallejo, Ricardo / Romero-Morales, Carlos / Palomo-López, Patricia / López-López, Daniel / Jiménez-Cebrián, Ana María

Journal of tissue viability

2022 Volume 32, Issue 1, Page(s) 26–32

Abstract: Background: The footwear assessment tool was designed to advise an appropriate footwear for each situation and patient. Footwear alterations structures can influence in musculoskeletal disorders, developing foot ulcers, increase the peak plantar ... ...

Abstract	Background: The footwear assessment tool was designed to advise an appropriate footwear for each situation and patient. Footwear alterations structures can influence in musculoskeletal disorders, developing foot ulcers, increase the peak plantar pressure, bacterial growth, low back pain. Methods: To validate the study 101 subjects were recruited. The study was tested by two expert podiatrists using the tool for the assessment of footwear characteristics that is composed by five domains, fit, general features, general structure, motion control properties and cushioning system. Each domain analyzes different shoe items. Results: An excellent agreement between the test-retest. A suitable Cronbach's α was suggested for the five domains of fit (α = 0.952), general features (α = 0.953), general structure (α = 0.947), motion control properties (α = 0.951), and cushioning system (α = 0.951). Test-retest reliability was excellent for all domains. There were no significant differences between any domain (p > 0.05). There was only statistically significant difference in the item forefoot height (p = 0.011). For all the domains items there were no statistically significant difference (p > 0.05). Conclusions: The tool for the assessment European footwear is a suitable repeatability and reliability footwear tool that can be used in Spanish language subjects.
MeSH term(s)	Humans ; Reproducibility of Results ; Pressure ; Foot ; Shoes
Language	English
Publishing date	2022-12-20
Publishing country	England
Document type	Journal Article
ZDB-ID	1282604-2
ISSN	0965-206X
ISSN	0965-206X
DOI	10.1016/j.jtv.2022.12.006
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Zs.A 4357: Show issues

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Article: Role of Female Research at the Asociacion Mexicana de Cirugia General Annual Meeting: A Retrospective Analysis From 2013 to 2019.

Mejía-Fernández, Lorelí / Romero-Hernández, Fernanda / López-Ruiz, Ana / Lopez-Verdugo, Fidel / Sanchez-Garcia, Jorge / Martinez-Ordaz, Jose L / Moreno-Paquentin, Eduardo / Lopez-Gavito, Elena

Frontiers in surgery

2022 Volume 9, Page(s) 900076

Abstract: Background: Academic surgery has been a traditionally male-dominated field. Female contribution remains challenging. In Mexico, there is no published evidence regarding gender disparity in academic surgery. We aimed to analyze the female role in ... ...

Abstract	Background: Academic surgery has been a traditionally male-dominated field. Female contribution remains challenging. In Mexico, there is no published evidence regarding gender disparity in academic surgery. We aimed to analyze the female role in clinical research submitted to the Asociación Mexicana de Cirugía General (AMCG). Methods: Retrospective study evaluating abstracts submitted to AMCG annual meetings from 2013 to 2019. Categorical variables were compared using χ Results: Overall, 7,439 abstracts were analyzed of which 24.2% were submitted by females. Female-submitted abstracts increased from 22.5% to 25.3% during 2013-2019 ( Conclusion: In Mexico, the female role in academic surgery is still limited. These results should 55 encourage professors and program directors to identify and address factors contributing to gender 56 disparities.
Language	English
Publishing date	2022-05-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2773823-1
ISSN	2296-875X
ISSN	2296-875X
DOI	10.3389/fsurg.2022.900076
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Article ; Online: Effectiveness of a multimodal intervention for improving chronic pain care.

Ruiz Romero, María Victoria / López Tarrida, Ángeles Carmen / Porrúa Del Saz, Ana / Gómez Hernández, María Begoña / Martínez Monrobé, María Blanca / Sánchez Villar, Elena / Cruz Valero, Carlos / Pereira Delgado, Consuelo

Revista espanola de salud publica

2023 Volume 97

Abstract: Objective: The prevalence of chronic pain in Spain is 17%, which causes suffering and significant loss of quality of life. Therapies should not focus only on pain reduction, to improve function and quality of life are necessary. Currently, it is ... ...

Title translation	Efectividad de una intervención multimodal para la mejora de la atención al dolor crónico.
Abstract	Objective: The prevalence of chronic pain in Spain is 17%, which causes suffering and significant loss of quality of life. Therapies should not focus only on pain reduction, to improve function and quality of life are necessary. Currently, it is committed to combining drugs and other therapies such as relaxation, meditation, cognitive behavioral therapy, targeted exercises, healthy lifestyles and techniques to increase self-esteem and motivation for change. These therapies can be used as part of a multimodal approach, forming part of multicomponent programs or workshops. In this paper we proposed to evaluate the effectiveness of a workshop developed from the Hospital San Juan de Dios del Ajarafe, that integrated different non-pharmacological therapies in the control of chronic non-oncologic pain and to analyze patients' perceptions of the techniques applied and how they affected pain and lifestyles. Methods: An intragroup before-after study (beginning-end of the workshop) was carried out, comparing pain, well-being, drug use, quality of life, and self-esteem. Surveys were conducted to deepen more qualitative aspects and identify improvements. The workshop consisted of psychoeducational action and training for the mental control of pain and emotions, based on the active participation of the patient in the management of his disease, promoting self-care and self-esteem, and aiming to improve well-being and quality of life. It consisted of 5 sessions of 3 hours (1 per week); of a group, interactive and practical nature. Statistical analysis was performed with SPSS vs.27.0. To compare related measures (before-after) we used Student's T tests for paired samples and Wilcoxon's test and to compare independent groups, Student's T and Mann Whitney's U; for the qualitative variables, we used Chi-Square and Fisher's test. Results: Four workshops were evaluated in which 63 patients participated, with a mean age of 57.6 (SD:11.37) years, 60 (95.2%) of them were women. At the end of the workshop pain decreased 1.5 median (-2.0-0) and well-being increased 2.0 (0-2.0); quality of life increased a median of 0.121 (SD: 0.209), health status 16.8 (23.78) and self-esteem 2.74 (4.73); [p<0.001]. The best valued techniques were meditations, mainly mental analgesia, affirmations in the mirror and self-esteem techniques. Conclusions: Overall satisfaction with the workshop is 9.8 out of 10. There is a pain control and improvement in quality of life, self-perception of health status, well-being and self-esteem.
MeSH term(s)	Humans ; Female ; Middle Aged ; Male ; Quality of Life ; Spain ; Pain ; Pain Management ; Life Style ; Retrospective Studies
Language	Spanish
Publishing date	2023-09-06
Publishing country	Spain
Document type	English Abstract ; Journal Article
ZDB-ID	1288657-9
ISSN	2173-9110 ; 0034-8899 ; 1135-5727
ISSN (online)	2173-9110
ISSN	0034-8899 ; 1135-5727
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