LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 181

Search options

  1. Article ; Online: 2-Selenouridine, a Modified Nucleoside of Bacterial tRNAs, Its Reactivity in the Presence of Oxidizing and Reducing Reagents.

    Kulik, Katarzyna / Sadowska, Klaudia / Wielgus, Ewelina / Pacholczyk-Sienicka, Barbara / Sochacka, Elzbieta / Nawrot, Barbara

    International journal of molecular sciences

    2022  Volume 23, Issue 14

    Abstract: The 5-substituted 2-selenouridines are natural components of the bacterial tRNA epitranscriptome. Because selenium-containing biomolecules are redox-active entities, the oxidation susceptibility of 2-selenouridine (Se2U) was studied in the presence of ... ...

    Abstract The 5-substituted 2-selenouridines are natural components of the bacterial tRNA epitranscriptome. Because selenium-containing biomolecules are redox-active entities, the oxidation susceptibility of 2-selenouridine (Se2U) was studied in the presence of hydrogen peroxide under various conditions and compared with previously reported data for 2-thiouridine (S2U). It was found that Se2U is more susceptible to oxidation and converted in the first step to the corresponding diselenide (Se2U)
    MeSH term(s) Indicators and Reagents ; Nucleosides ; Organoselenium Compounds ; Oxidation-Reduction ; RNA, Transfer/metabolism ; Reducing Agents ; Selenium ; Uridine/analogs & derivatives ; Uridine/metabolism
    Chemical Substances 2-selenouridine ; Indicators and Reagents ; Nucleosides ; Organoselenium Compounds ; Reducing Agents ; RNA, Transfer (9014-25-9) ; Selenium (H6241UJ22B) ; Uridine (WHI7HQ7H85)
    Language English
    Publishing date 2022-07-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms23147973
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Can primary care physicians recognize type 1 diabetes in children on time?

    Wójcik, Małgorzata / Wasyl-Nawrot, Barbara / Starzyk, Jerzy

    Pediatric endocrinology, diabetes, and metabolism

    2021  Volume 27, Issue 4, Page(s) 245–248

    Abstract: Introduction: Although the diagnostic criteria for diabetes in children and adolescents should be widely known to all healthcare professionals, many cases of type 1 diabetes (DM1) are still not recognized in time. The aim of this study was to compare ... ...

    Title translation Czy lekarze podstawowej opieki zdrowotnej potrafią na czas rozpoznać cukrzycę typ 1 u dzieci?
    Abstract Introduction: Although the diagnostic criteria for diabetes in children and adolescents should be widely known to all healthcare professionals, many cases of type 1 diabetes (DM1) are still not recognized in time. The aim of this study was to compare the experiences with the diagnosis of DM1 of young doctors during training and specialists working as primary health care physicians in Poland.
    Material and methods: 50 specialists in paediatrics or family medicine (average professional experience of 14.6 years) and 50 doctors, and those without specialization (up to 4 years after graduating from medical studies, average professional experience of 1.4 years) participated in the survey. The questionnaires were conducted from November 2019 to November 2020.
    Results: Most of the study participants correctly answered the questions about the diagnostic criteria of diabetes and its management at the moment of diagnosis, except for the question regarding diagnostic criteria for diabetes in oral glucose tolerance test. The correct answers varied from 96% to 72% among medical specialists and physicians during the training, respectively. What is interesting is the relatively small number of diagnosed cases of DM1 during the entire professional career of specialists (0-12 cases), compared to the doctors without specialization (0-5 cases). Medical specialists declared more frequently the use of a glucometer in their daily practice (mean 6 vs. 1, p < 0.05), but after taking into account the years of experience, the difference was insignificant.
    Conclusions: It is important to educate primary care physicians, despite their professional experience. Practical education of physicians is also necessary.
    MeSH term(s) Adolescent ; Blood Glucose Self-Monitoring ; Child ; Diabetes Mellitus, Type 1/diagnosis ; Diabetes Mellitus, Type 1/therapy ; Family Practice ; Humans ; Physicians, Primary Care ; Surveys and Questionnaires
    Language English
    Publishing date 2021-11-16
    Publishing country Poland
    Document type Journal Article
    ZDB-ID 2617120-X
    ISSN 2083-8441 ; 2083-8441
    ISSN (online) 2083-8441
    ISSN 2083-8441
    DOI 10.5114/pedm.2021.107723
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: EGFR-Targeted Cellular Delivery of Therapeutic Nucleic Acids Mediated by Boron Clusters.

    Kaniowski, Damian / Suwara, Justyna / Ebenryter-Olbińska, Katarzyna / Jakóbik-Kolon, Agata / Nawrot, Barbara

    International journal of molecular sciences

    2022  Volume 23, Issue 23

    Abstract: New boron carriers with high boron content and targeted cancer-cell delivery are considered the first choice for boron neutron capture therapy (BNCT) for cancer treatment. Previously, we have shown that composites of antisense oligonucleotide and boron ... ...

    Abstract New boron carriers with high boron content and targeted cancer-cell delivery are considered the first choice for boron neutron capture therapy (BNCT) for cancer treatment. Previously, we have shown that composites of antisense oligonucleotide and boron clusters are functional nanoparticles for the downregulation of expression of epidermal growth factor receptor (EGFR) and can be loaded into EGFR-overexpressing cancer cells without a transfection factor. In this study, we hypothesize that free cellular uptake is mediated by binding and activation of the EGFR by boron clusters. Proteomic analysis of proteins pulled-down from various EGFR-overexpressing cancer cells using short oligonucleotide probes, conjugated to 1,2-dicarba-
    Language English
    Publishing date 2022-11-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms232314793
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Boron Clusters as Enhancers of RNase H Activity in the Smart Strategy of Gene Silencing by Antisense Oligonucleotides.

    Kaniowski, Damian / Kulik, Katarzyna / Suwara, Justyna / Ebenryter-Olbińska, Katarzyna / Nawrot, Barbara

    International journal of molecular sciences

    2022  Volume 23, Issue 20

    Abstract: Boron cluster-conjugated antisense oligonucleotides (B-ASOs) have already been developed as therapeutic agents with "two faces", namely as potential antisense inhibitors of gene expression and as boron carriers for boron neutron capture therapy (BNCT). ... ...

    Abstract Boron cluster-conjugated antisense oligonucleotides (B-ASOs) have already been developed as therapeutic agents with "two faces", namely as potential antisense inhibitors of gene expression and as boron carriers for boron neutron capture therapy (BNCT). The previously observed high antisense activity of some B-ASOs targeting the epidermal growth factor receptor (EGFR) could not be rationally assigned to the positioning of the boron cluster unit: 1,2-dicarba-
    MeSH term(s) Oligonucleotides, Antisense/pharmacology ; Boron/metabolism ; Kinetics ; RNA, Complementary ; Ribonuclease H/genetics ; Ribonuclease H/metabolism ; Gene Silencing ; Oligonucleotides ; ErbB Receptors/metabolism ; Pyrimidines ; Iron/metabolism
    Chemical Substances Oligonucleotides, Antisense ; Boron (N9E3X5056Q) ; RNA, Complementary ; Ribonuclease H (EC 3.1.26.4) ; Oligonucleotides ; ErbB Receptors (EC 2.7.10.1) ; Pyrimidines ; Iron (E1UOL152H7)
    Language English
    Publishing date 2022-10-13
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms232012190
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: Corrigendum to "Biochemical, crystallographic and biophysical characterization of histidine triad nucleotide-binding protein 2 with different ligands including a non-hydrolyzable analog of Ap4A" [BBA - General Subjects 1865 (2021) 129968-129983].

    Dolot, Rafał / Krakowiak, Agnieszka / Kaczmarek, Renata / Włodarczyk, Artur / Pichlak, Marta / Nawrot, Barbara

    Biochimica et biophysica acta. General subjects

    2021  Volume 1866, Issue 2, Page(s) 130035

    Language English
    Publishing date 2021-10-24
    Publishing country Netherlands
    Document type Published Erratum
    ZDB-ID 60-7
    ISSN 1872-8006 ; 1879-2596 ; 1879-260X ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    ISSN (online) 1872-8006 ; 1879-2596 ; 1879-260X ; 1879-2642 ; 1879-2618 ; 1879-2650
    ISSN 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    DOI 10.1016/j.bbagen.2021.130035
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.247: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: Different Oxidation Pathways of 2-Selenouracil and 2-Thiouracil, Natural Components of Transfer RNA.

    Kulik, Katarzyna / Sadowska, Klaudia / Wielgus, Ewelina / Pacholczyk-Sienicka, Barbara / Sochacka, Elzbieta / Nawrot, Barbara

    International journal of molecular sciences

    2020  Volume 21, Issue 17

    Abstract: Sulfur- and selenium-modified uridines present in the wobble position of transfer RNAs (tRNAs) play an important role in the precise reading of genetic information and tuning of protein biosynthesis in all three domains of life. Both sulfur and selenium ... ...

    Abstract Sulfur- and selenium-modified uridines present in the wobble position of transfer RNAs (tRNAs) play an important role in the precise reading of genetic information and tuning of protein biosynthesis in all three domains of life. Both sulfur and selenium chalcogens functionally operate as key elements of biological molecules involved in the protection of cells against oxidative damage. In this work, 2-thiouracil (S2Ura) and 2-selenouracil (Se2Ura) were treated with hydrogen peroxide at 1:0.5, 1:1, and 1:10 molar ratios and at selected pH values ranging from 5 to 8. It was found that Se2Ura was more prone to oxidation than its sulfur analog, and if reacted with H
    MeSH term(s) Hydrogen Peroxide/chemistry ; Oxidation-Reduction ; Thiouracil/chemistry ; Uracil/analogs & derivatives ; Uracil/chemistry
    Chemical Substances 2-selenouracil (16724-03-1) ; Uracil (56HH86ZVCT) ; Thiouracil (59X161SCYL) ; Hydrogen Peroxide (BBX060AN9V)
    Language English
    Publishing date 2020-08-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms21175956
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article: In Vitro Gingival Wound Healing Activity of Extracts from

    Nawrot-Hadzik, Izabela / Matkowski, Adam / Pitułaj, Artur / Sterczała, Barbara / Olchowy, Cyprian / Szewczyk, Anna / Choromańska, Anna

    Pharmaceutics

    2021  Volume 13, Issue 11

    Abstract: ... Rhizomes ... ...

    Abstract Rhizomes of
    Language English
    Publishing date 2021-10-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527217-2
    ISSN 1999-4923
    ISSN 1999-4923
    DOI 10.3390/pharmaceutics13111764
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article ; Online: Synthesis and properties of the anticodon stem-loop of human mitochondrial tRNA

    Podskoczyj, Karolina / Kulik, Katarzyna / Wasko, Joanna / Nawrot, Barbara / Suzuki, Tsutomu / Leszczynska, Grazyna

    Chemical communications (Cambridge, England)

    2021  Volume 57, Issue 93, Page(s) 12540–12543

    Abstract: A single point mutation (A4435G) in the human mitochondrial ... ...

    Abstract A single point mutation (A4435G) in the human mitochondrial tRNA
    MeSH term(s) Codon ; Diabetes Mellitus, Type 2/genetics ; Diabetes Mellitus, Type 2/pathology ; Guanosine/analogs & derivatives ; Guanosine/chemistry ; Humans ; Hypertension/genetics ; Hypertension/pathology ; Mitochondria/metabolism ; Nucleic Acid Conformation ; Optic Atrophy, Hereditary, Leber/genetics ; Optic Atrophy, Hereditary, Leber/pathology ; RNA, Transfer, Met/chemistry ; RNA, Transfer, Met/genetics
    Chemical Substances Codon ; RNA, Transfer, Met ; Guanosine (12133JR80S) ; 1-methylguanosine (2140-65-0)
    Language English
    Publishing date 2021-11-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 1472881-3
    ISSN 1364-548X ; 1359-7345 ; 0009-241X
    ISSN (online) 1364-548X
    ISSN 1359-7345 ; 0009-241X
    DOI 10.1039/d1cc05215b
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  9. Article ; Online: Synthesis, crystallization and preliminary crystallographic analysis of a 52-nucleotide DNA/2'-OMe-RNA oligomer mimicking 10-23 DNAzyme in the complex with a substrate.

    Dolot, Rafał / Sobczak, Milena / Mikołajczyk, Barbara / Nawrot, Barbara

    Nucleosides, nucleotides & nucleic acids

    2017  Volume 36, Issue 4, Page(s) 292–301

    Abstract: A 52-nucleotide DNA/2'-OMe-RNA oligomer mimicking 10-23 DNAzyme in the complex with its substrate was synthesized, purified and crystallized by the hanging-drop method using 0.8 M sodium potassium tartrate as a precipitant. A data set to 1.21 Å ... ...

    Abstract A 52-nucleotide DNA/2'-OMe-RNA oligomer mimicking 10-23 DNAzyme in the complex with its substrate was synthesized, purified and crystallized by the hanging-drop method using 0.8 M sodium potassium tartrate as a precipitant. A data set to 1.21 Å resolution was collected from a monocrystal at 100 K using synchrotron radiation on a beamline BL14.1 at BESSY. The crystal belonged to the P2
    MeSH term(s) Base Sequence ; Biomimetic Materials/chemical synthesis ; Biomimetic Materials/chemistry ; Chemistry Techniques, Synthetic ; Crystallization ; DNA, Catalytic/metabolism ; Nucleotides/chemistry ; RNA/chemistry ; RNA/genetics
    Chemical Substances DNA, Catalytic ; Nucleotides ; RNA (63231-63-0)
    Language English
    Publishing date 2017-04-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2008956-9
    ISSN 1532-2335 ; 1525-7770
    ISSN (online) 1532-2335
    ISSN 1525-7770
    DOI 10.1080/15257770.2016.1276291
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 3870: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  10. Article ; Online: Escherichia coli tRNA 2-selenouridine synthase SelU selects its prenyl substrate to accomplish its enzymatic function.

    Szczupak, Patrycja / Radzikowska-Cieciura, Ewa / Kulik, Katarzyna / Madaj, Rafał / Sierant, Małgorzata / Krakowiak, Agnieszka / Nawrot, Barbara

    Bioorganic chemistry

    2022  Volume 122, Page(s) 105739

    Abstract: Bacterial tRNA 2-selenouridine synthase (SelU) in vitro converts S2U-RNA to its selenium analog (Se2U-RNA) in a two-step process: (i) geranylation of S2U-RNA (with geranyl pyrophosphate, gePP), and (ii) selenation of the resulting geS2U-RNA (with the ... ...

    Abstract Bacterial tRNA 2-selenouridine synthase (SelU) in vitro converts S2U-RNA to its selenium analog (Se2U-RNA) in a two-step process: (i) geranylation of S2U-RNA (with geranyl pyrophosphate, gePP), and (ii) selenation of the resulting geS2U-RNA (with the selenophosphate anion, SePO
    MeSH term(s) Escherichia coli/enzymology ; Escherichia coli/genetics ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/metabolism ; Neoprene ; Selenium ; Sulfurtransferases/genetics ; Sulfurtransferases/metabolism
    Chemical Substances Escherichia coli Proteins ; prenyl ; Neoprene (9010-98-4) ; Sulfurtransferases (EC 2.8.1.-) ; tRNA 2-selenouridine synthase (EC 2.8.1.-) ; Selenium (H6241UJ22B)
    Language English
    Publishing date 2022-03-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 120080-x
    ISSN 1090-2120 ; 0045-2068
    ISSN (online) 1090-2120
    ISSN 0045-2068
    DOI 10.1016/j.bioorg.2022.105739
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 4207: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top