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Book: TGF-ß signaling / [1] / ed. by Xin-Hua Feng ; Pinglong Xu ; Xia Lin

Feng, Xin-Hua / Zi, Zhike

methods and protocols

(Methods in molecular biology ; 1344)

2016

Author's details	edited by Xin-Hua Feng, Zhike Zi [und weiteren]
Series title	Methods in molecular biology ; 1344 TGF-ß signaling
Collection	TGF-ß signaling
Language	English
Size	xii, 394 Seiten., Illustrationen, Diagramme
Publishing country	United States
Document type	Book
HBZ-ID	HT018812171
ISBN	978-1-4939-2965-8 ; 9781493929665 ; 1-4939-2965-8 ; 1493929666
Database	Catalogue ZB MED Medicine, Health

In stock of ZB MED Cologne/Königswinter

Shelf mark	Loan status	Location
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Article ; Online: Xin-Ji-Er-Kang protects myocardial and renal injury in hypertensive heart failure in mice.

Ling, Xin-Xin / Chen, Hua / Fu, Bei-Bei / Ruan, Cheng-Shao / Pana, Ming / Zhou, Kai / Fang, Zhi-Rui / Shao, Jun-Tang / Zhu, Feng-Qin / Gao, Shan

Phytomedicine : international journal of phytotherapy and phytopharmacology

2021 Volume 91, Page(s) 153675

Abstract: Background: Xin-Ji-Er-Kang (XJEK) as a herbal formula of traditional Chinese medicine (TCM) has ...

Abstract	Background: Xin-Ji-Er-Kang (XJEK) as a herbal formula of traditional Chinese medicine (TCM) has shown the protective effects on myocardial function as well as renal function in mouse models of myocardial infarction. Hypothesis/purpose: We investigated the effects of XJEK on cardiovascular- and renal-function in a heart failure mouse model induced by high salt (HS) and the associated mechanisms. Study design: For the purpose of assessing the effects of XJEK on a hypertensive heart failure model, mice were fed with 8% high salt diet. XJEK was administered by oral gavage for 8 weeks. Cardiovascular function parameters, renal function associated biomarkers and XJEK's impact on renin-angiotensin-aldosterone system (RAAS) activation were assessed. To determine the underlying mechanism, the calpain1/junctophilin-2 (JP2)/sarcoplasmic reticulum Ca Results: Mice on HS-diet exhibited hypertensive heart failure along with progressive kidney injury. Similar to fosinopril, XJEK ameliorated hypertension, cardiovascular-and renal- dysfunction in mice of HS-diet group. XJEK inhibited HS-induced activation of RAAS and reversed the abnormal expression pattern of calpain1and JP2 protein in heart tissues. XJEK significantly improved cell viability of angiotensin II-challenged AC16 cells. Moreover, XJEK's impact on calpain1/JP2 pathway was partly diminished in AC16 cells transfected with calpastatin siRNA. Conclusion: XJEK was found to exert cardiovascular- and renal protection in HS-diet induced heart failure mouse model. XJEK inhibited HS-diet induced RAAS activation by inhibiting the activity and expression of calpain1 and protected the junctional membrane complex (JMC) in cardiomyocytes.
MeSH term(s)	Animals ; Blood Pressure ; Calpain ; Drugs, Chinese Herbal/pharmacology ; Heart Failure/drug therapy ; Hypertension/drug therapy ; Kidney/drug effects ; Kidney/physiology ; Membrane Proteins ; Mice ; Muscle Proteins ; Oxidative Stress ; Sarcoplasmic Reticulum Calcium-Transporting ATPases ; Signal Transduction
Chemical Substances	Drugs, Chinese Herbal ; Membrane Proteins ; Muscle Proteins ; junctophilin-2 protein, mouse ; xin-ji-er-kang ; Calpain (EC 3.4.22.-) ; Capn1 protein, mouse (EC 3.4.22.52) ; Sarcoplasmic Reticulum Calcium-Transporting ATPases (EC 3.6.3.8) ; Atp2a2 protein, mouse (EC 7.2.2.10)
Language	English
Publishing date	2021-07-18
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2021.153675
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Article ; Online: Study on the chemical constituents and mechanism of Kai-Xin-San based on UPLC-Q-Exactive MS and network pharmacology.

Shang, Bingxian / Jia, Shuhe / Zhang, Tong / Gao, Feng / Lu, Mingjun / Chen, Kedian / Jiao, Jingyi / Dai, Ziqi / Zeng, Qi / Xu, Bing / Lei, Haimin

Journal of ethnopharmacology

2023 Volume 322, Page(s) 117652

Abstract: ... the characteristics of multi-components and multi-targets to treat diseases. Kai-Xin-San is a TCM formula applied ... of the study: To explore the effective components of Kai-Xin-San, investigate the effect of Kai-Xin-San ... on angiogenesis, screen and verify the related targets and possible mechanisms of Kai-Xin-San against VD ...

Abstract	Ethnopharmacological relevance: Vascular disease (VD) is a kind of common disease harmful to the health of the middle-aged and elderly, which has the characteristics of long treatment cycle and high recurrence rate, and without effective method to treat so far. Traditional Chinese medicine (TCM) has the characteristics of multi-components and multi-targets to treat diseases. Kai-Xin-San is a TCM formula applied for treating psychiatric diseases such as depression in China for thousands of years, and it has been used in clinical treatment of VD. But up to now, its active composition and mechanism are not clear. Aim of the study: To explore the effective components of Kai-Xin-San, investigate the effect of Kai-Xin-San on angiogenesis, screen and verify the related targets and possible mechanisms of Kai-Xin-San against VD. Materials and methods: UPLC-Q-Exactive Orbitrap MS was performed to identify the chemical components of Kai-Xin-San. The mechanism of multi-components, multi-targets, and multi-pathways of Kai-Xin-San in the treatment of VD were explored by network pharmacology. And then, quail chick chorioallantoic membrane (qCAM) assays were used to evaluate the vascular protective activity of Kai-Xin-San. Evaluation of angiogenesis by calculating the relative vessels area. The levels of VEGFA and Akt1 in qCAM were measured by RT-PCR. Twenty-five male SD rats were randomly divided into the sham group, model group, Donepezil (0.45 mg/kg) group, Kai-Xin-San low dose group (0.1575 g/kg), Kai-Xin-San high dose group (0.63 g/kg). Two-vessel occlusion (2-VO) rat model is established to evaluate the therapeutic effect of Kai-Xin-San pretreatment. Hematoxylin-eosin (HE) staining is conducted to detect the morphological changes of neurons in the hippocampus. Results: Data showed that 62 compounds were identified in Kai-Xin-San. The network pharmacology results showed 73 compounds in Kai-Xin-San play a role in the treatment of VD, such as Ginsenoside Rh Conclusion: The complex chemical components of Kai-Xin-San play a synergistic role in the treatment of VD, and involve multiple pathways and targets. To protect blood vessels by promoting angiogenesis is one of the potential mechanisms of Kai-Xin-San in the treatment of VD. This study reveals that Kai-Xin-San protects the 2-VO model rats from ischemic injury by alleviating neuron damage in the hippocampus.
MeSH term(s)	Humans ; Aged ; Middle Aged ; Rats ; Male ; Animals ; Chromatography, High Pressure Liquid/methods ; Rats, Sprague-Dawley ; Network Pharmacology ; Phosphatidylinositol 3-Kinases ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Drugs, Chinese Herbal/analysis ; Molecular Docking Simulation
Chemical Substances	Kai-Xin-San ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Drugs, Chinese Herbal
Language	English
Publishing date	2023-12-25
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.117652
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Article ; Online: Toyoncin, a Novel Leaderless Bacteriocin That Is Produced by Bacillus toyonensis XIN-YC13 and Specifically Targets B. cereus and Listeria monocytogenes.

Wang, Juanjuan / Xu, Haitao / Liu, Shu / Song, Baolong / Liu, Hualin / Li, Feng / Deng, Shulin / Wang, Guangli / Zeng, Huawei / Zeng, Xin / Xu, Dayong / Zhang, Biao / Xin, Bingyue

Applied and environmental microbiology

2021 Volume 87, Issue 12, Page(s) e0018521

Abstract: ... a laboratory-based screening strategy, we identified a strain in the B. cereus group, Bacillus toyonensis XIN ... toyoncin, was purified from the culture supernatant of strain XIN-YC13, and its molecular mass was found ...

Abstract	Bacteriocins have attracted increasing interest because of their potential as natural preservatives. Recent studies showed that the Bacillus cereus group is a prominent producer of bacteriocins. Using a laboratory-based screening strategy, we identified a strain in the B. cereus group, Bacillus toyonensis XIN-YC13, with antimicrobial activity against B. cereus. A novel, 70-amino-acid-long leaderless bacteriocin, toyoncin, was purified from the culture supernatant of strain XIN-YC13, and its molecular mass was found to be 7,817.1012 Da. Toyoncin shares no similarity with any other known bacteriocins, and its N-terminal amino acid is formylmethionine rather than methionine. Toyoncin shows good pH and heat stability and exhibits specific antimicrobial activity against two important foodborne pathogens, B. cereus and Listeria monocytogenes. Additionally, toyoncin exerts bactericidal activity and induces cell membrane damage. Toyoncin can also inhibit the outgrowth of B. cereus spores. Preservation assays showed that toyoncin effectively suppressed or eradicated B. cereus and L. monocytogenes in pasteurized skim milk. These results suggest that toyoncin can be used as a new biopreservative against B. cereus and L. monocytogenes in the food industry.
MeSH term(s)	Amino Acid Sequence ; Animals ; Bacillus/metabolism ; Bacillus cereus/drug effects ; Bacillus cereus/growth & development ; Bacteriocins/chemistry ; Bacteriocins/genetics ; Bacteriocins/isolation & purification ; Bacteriocins/pharmacology ; Biological Control Agents ; Food Microbiology ; Food Preservatives/chemistry ; Food Preservatives/isolation & purification ; Food Preservatives/pharmacology ; Hydrogen-Ion Concentration ; Listeria monocytogenes/drug effects ; Listeria monocytogenes/growth & development ; Milk/microbiology ; Multigene Family ; Spores, Bacterial/drug effects ; Spores, Bacterial/growth & development ; Temperature
Chemical Substances	Bacteriocins ; Biological Control Agents ; Food Preservatives
Language	English
Publishing date	2021-05-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	223011-2
ISSN	1098-5336 ; 0099-2240
ISSN (online)	1098-5336
ISSN	0099-2240
DOI	10.1128/AEM.00185-21
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Article ; Online: Kai-Xin-San Improves Cognitive Impairment via Wnt/β-Catenin and IRE1/XBP1s Signalings in APP/PS1 Mice.

Xu, Yu-Min / Lu, Fang-Mei / Xu, Hong-Cai / Zhang, Jie / Hei, Shang-Yan / Qiu, Yu-Hui / Cai, Ye-Feng / Zhang, Shi-Jie / Zhao, Min

Rejuvenation research

2023 Volume 26, Issue 3, Page(s) 105–115

Abstract: ... progression. Kai-Xin-San (KXS) has been reported to be effective in improving cognitive impairment in AD ...

Abstract	Alzheimer's disease (AD) is the most common type of dementia with an insidious onset and slow progression. Kai-Xin-San (KXS) has been reported to be effective in improving cognitive impairment in AD. However, the mechanism is still confused. In this study, we employed APP/PS1 mice to explore the neuroprotective mechanism of KXS. Forty-eight male APP/PS1 mice were randomly divided into model group, KXS groups (0.7, 1.4, and 2.8 g/kg/d, p.o.) and the wild-type mice were assigned to the normal control group (
MeSH term(s)	Animals ; Male ; Mice ; Alzheimer Disease/drug therapy ; Alzheimer Disease/metabolism ; beta Catenin ; Cognitive Dysfunction/drug therapy ; Disease Models, Animal ; Glycogen Synthase Kinase 3 beta ; Mice, Transgenic ; Protein Serine-Threonine Kinases ; Wnt Signaling Pathway/drug effects
Chemical Substances	beta Catenin ; Glycogen Synthase Kinase 3 beta (EC 2.7.11.1) ; Kai-Xin-San ; Protein Serine-Threonine Kinases (EC 2.7.11.1) ; Ern2 protein, mouse (EC 2.7.1.-) ; Xbp1 protein, mouse
Language	English
Publishing date	2023-05-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	2150779-X
ISSN	1557-8577 ; 1549-1684
ISSN (online)	1557-8577
ISSN	1549-1684
DOI	10.1089/rej.2022.0063
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Article: Xin-Ji-Er-Kang Alleviates Isoproterenol-Induced Myocardial Hypertrophy in Mice through the Nrf2/HO-1 Signaling Pathway.

Yu, Ting-Ting / Sun, Li-Jun / Chen, Chen / Wang, Zi-Jian / Liu, Xue-Sheng / Zhu, Feng-Qin / Gao, Shan

Evidence-based complementary and alternative medicine : eCAM

2022 Volume 2022, Page(s) 7229080

Abstract: Xin-Ji-Er-Kang (XJEK) inhibited cardiovascular remodeling in hypertensive mice in our previous ...

Abstract	Xin-Ji-Er-Kang (XJEK) inhibited cardiovascular remodeling in hypertensive mice in our previous studies. We hypothesized that XJEK may prevent isoproterenol (ISO)-induced myocardial hypertrophy (MH) in mice by ameliorating oxidative stress (OS) through a mechanism that may be related to the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1(HO-1) pathways. Forty SPF male Kunming mice were randomized into 5 groups (
Language	English
Publishing date	2022-08-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	2171158-6
ISSN	1741-4288 ; 1741-427X
ISSN (online)	1741-4288
ISSN	1741-427X
DOI	10.1155/2022/7229080
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Article: Xin-Ji-Er-Kang protects heart from ischemia-reperfusion injury by rebalancing lipid metabolism.

Sun, Li-Jun / Wang, Xiao-Yu / Xia, Jie / Xu, Yan-Mei / Liao, Yu-Feng / Qin, Yuan-Yuan / Ge, Xue-Wan / Zhao, Pei-Wen / Xu, Tong / Zhu, Xiao-Ling / Gao, Shan / Xiao, Rui / Liu, Xue-Sheng / Zhou, Kai

Frontiers in pharmacology

2022 Volume 13, Page(s) 981766

Abstract: Background and Purpose: ...

Abstract	Background and Purpose:
Language	English
Publishing date	2022-08-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2022.981766
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Article: Identification and characterization of a novel circular bacteriocin, bacicyclicin XIN-1, from Bacillus sp. Xin1

Xin, Bingyue / Xu, Haitao / Liu, Hualin / Liu, Shu / Wang, Juanjuan / Xue, Jianping / Zhang, Fei / Deng, Shulin / Zeng, Huawei / Zeng, Xin / Xu, Dayong / Zhao, Yi / Li, Feng / Wang, Guangli

Food control. 2021 Mar., v. 121

2021

Abstract: ... several food-borne pathogens. A novel circular bacteriocin, bacicyclicin XIN-1 (MW: 5848.1719 Da), was purified ... Staphylococcus aureus, three important foodborne pathogens, as well as other pathogens. Bacicyclicin XIN-1 also ... inhibited the outgrowth of Bacillus cereus spores and did not show hemolysis activity. Bacicyclicin XIN-1 ...

Abstract	In recent years, bacteriocins have attracted considerable interest for use as natural food preservatives against spoilage and pathogenic microorganisms. Recent studies have shown that the strains of Bacillus cereus group produce a range of highly diverse bacteriocins. In this study, we screened a large number of Bacillus cereus group strains and isolated one strain, Bacillus sp. Xin1, with antimicrobial activity against several food-borne pathogens. A novel circular bacteriocin, bacicyclicin XIN-1 (MW: 5848.1719 Da), was purified from the culture supernatant of Bacillus sp. Xin1. This bacteriocin exhibited high thermal stability, wide pH tolerance, and a wide range of antimicrobial activities against Bacillus cereus, Listeria monocytogenes, and Staphylococcus aureus, three important foodborne pathogens, as well as other pathogens. Bacicyclicin XIN-1 also inhibited the outgrowth of Bacillus cereus spores and did not show hemolysis activity. Bacicyclicin XIN-1 effectively inhibited or eliminated B. cereus ATTCC14579, S. aureus ATCC6538, and L. monocytogenes LM201 in skim milk. Our findings indicate that bacicyclicin XIN-1 has a remarkable potential for use as a natural food preservative in the food industry.
Keywords	Bacillus cereus ; Listeria monocytogenes ; Staphylococcus aureus ; antimicrobial properties ; bacteriocins ; food industry ; food pathogens ; food preservatives ; food safety ; hemolysis ; microorganisms ; pH ; skim milk ; spoilage ; spores ; strains ; thermal stability
Language	English
Dates of publication	2021-03
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	1027805-9
ISSN	0956-7135
ISSN	0956-7135
DOI	10.1016/j.foodcont.2020.107696
Database	NAL-Catalogue (AGRICOLA)

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Article: Xin-Ji-Er-Kang protects myocardial and renal injury in hypertensive heart failure in mice

Ling, Xin-xin / Chen, Hua / Fu, Bei-bei / Ruan, Cheng-shao / Pana, Ming / Zhou, Kai / Fang, Zhi-rui / Shao, Jun-tang / Zhu, Feng-qin / Gao, Shan

Phytomedicine. 2021 Oct., v. 91

2021

Abstract: Xin-Ji-Er-Kang (XJEK) as a herbal formula of traditional Chinese medicine (TCM) has shown ...

Abstract	Xin-Ji-Er-Kang (XJEK) as a herbal formula of traditional Chinese medicine (TCM) has shown the protective effects on myocardial function as well as renal function in mouse models of myocardial infarction.We investigated the effects of XJEK on cardiovascular- and renal-function in a heart failure mouse model induced by high salt (HS) and the associated mechanisms.For the purpose of assessing the effects of XJEK on a hypertensive heart failure model, mice were fed with 8% high salt diet. XJEK was administered by oral gavage for 8 weeks. Cardiovascular function parameters, renal function associated biomarkers and XJEK's impact on renin-angiotensin–aldosterone system (RAAS) activation were assessed. To determine the underlying mechanism, the calpain1/junctophilin-2 (JP2)/sarcoplasmic reticulum Ca²⁺ ATPase (SERCA2a) pathway was further studied in AC16 cells after angiotensin II-challenge or after calpastatin small interfering RNA (siRNA) transfection.Mice on HS-diet exhibited hypertensive heart failure along with progressive kidney injury. Similar to fosinopril, XJEK ameliorated hypertension, cardiovascular-and renal- dysfunction in mice of HS-diet group. XJEK inhibited HS-induced activation of RAAS and reversed the abnormal expression pattern of calpain1and JP2 protein in heart tissues. XJEK significantly improved cell viability of angiotensin II-challenged AC16 cells. Moreover, XJEK's impact on calpain1/JP2 pathway was partly diminished in AC16 cells transfected with calpastatin siRNA.XJEK was found to exert cardiovascular- and renal protection in HS-diet induced heart failure mouse model. XJEK inhibited HS-diet induced RAAS activation by inhibiting the activity and expression of calpain1 and protected the junctional membrane complex (JMC) in cardiomyocytes.
Keywords	Oriental traditional medicine ; adenosinetriphosphatase ; biomarkers ; calcium ; calpastatin ; cardiomyocytes ; cell viability ; diet ; heart failure ; hypertension ; kidneys ; mice ; renal function ; renoprotective effect ; reticulum
Language	English
Dates of publication	2021-10
Publishing place	Elsevier GmbH
Document type	Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2021.153675
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Targeted pharmacokinetics and bioinformatics screening strategy reveals JAK2 as the main target for Xin-Ji-Er-Kang in treatment of MIR injury.

Zhou, Kai / Chen, Hua / Wang, Xiao-Yu / Xu, Yan-Mei / Liao, Yu-Feng / Qin, Yuan-Yuan / Ge, Xue-Wan / Zhang, Ting-Ting / Fang, Zhong-Lin / Fu, Bei-Bei / Xiao, Qing-Zhong / Zhu, Feng-Qin / Chen, Si-Rui / Liu, Xue-Sheng / Luo, Qi-Chao / Gao, Shan

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

2022 Volume 155, Page(s) 113792

Abstract: Background and purpose: Xin-Ji-Er-Kang (XJEK) is traditional Chinese formula presented excellent ...

Abstract	Background and purpose: Xin-Ji-Er-Kang (XJEK) is traditional Chinese formula presented excellent protective effects on several heart diseases, but the potential components and targets are still unclear. The aim of this study is to elucidate the effective components of XJEK and reveal its potential mechanism of cardioprotective effect in myocardial ischemia-reperfusion (MIR) injury. Experimental approach: Firstly, the key compounds in XJEK, plasma and heart tissue were analyzed by high resolution mass spectrometry. Bioinformatics studies were also involved to disclose the potential targets and the binding sites for the key compounds. Secondly, to study the protective effect of XJEK on MIR injury and related mechanism, mice subjected to MIR surgery and gavage administered with XJEK for 6 weeks. Cardiac function parameters and apoptosis level of cardiac tissue were assessed. The potential mechanism was further verified by knock down of target protein in vitro. Results: Pharmacokinetics studies showed that Sophora flavescens alkaloids, primarily composed with matrine, are the key component of XJEK. And, through bioinformatic analysis, we speculated JAK2 could be the potential target for XJEK, and could form stable hydrogen bonds with matrine. Administration of XJEK and matrine significantly improved heart function and reduced apoptosis of cardiomyocytes by increasing the phosphorylation of JAK2 and STAT3. The anti-apoptosis effect of XJEK and matrine was also observed on AC16 cells, and could be reversed by co-treatment with JAK2 inhibitor AG490 or knock-down of JAK2. Conclusion: XJEK exerts cardioprotective effect on MIR injury, which may be associated with the activation of JAK2/STAT3 signaling pathway.
MeSH term(s)	Animals ; Mice ; Myocardial Reperfusion Injury/drug therapy ; Myocardial Reperfusion Injury/genetics ; Myocardial Reperfusion Injury/metabolism ; Computational Biology ; Janus Kinase 2/metabolism ; STAT3 Transcription Factor/metabolism ; Alkaloids ; Myocytes, Cardiac/metabolism
Chemical Substances	xin-ji-er-kang ; Janus Kinase 2 (EC 2.7.10.2) ; STAT3 Transcription Factor ; Alkaloids
Language	English
Publishing date	2022-10-07
Publishing country	France
Document type	Journal Article
ZDB-ID	392415-4
ISSN	1950-6007 ; 0753-3322 ; 0300-0893
ISSN (online)	1950-6007
ISSN	0753-3322 ; 0300-0893
DOI	10.1016/j.biopha.2022.113792
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