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Article: Kinetic modelling of 2,3-butanediol production by Raoultella terrigena CECT 4519 resting cells: Effect of fluid dynamics conditions and initial glycerol concentration

Ripoll, Vanessa / Ladero, Miguel / Santos, Victoria E.

Biochemical engineering journal. 2021 Dec., v. 176

2021

Abstract: Biodiesel-derived glycerol was biologically converted to 2,3-butanediol, an organic compound with multiple industrial applications. Recent studies showed that Raoultella terrigena CECT 4519 is an effective biocatalyst for the bioprocess under ... ...

Abstract	Biodiesel-derived glycerol was biologically converted to 2,3-butanediol, an organic compound with multiple industrial applications. Recent studies showed that Raoultella terrigena CECT 4519 is an effective biocatalyst for the bioprocess under conventional growing conditions. In the present work, a novel biocatalyst composed by R. terrigena resting cells was evaluated for the first time. Fluid dynamic conditions has been optimized to maximize 2,3-butanediol production in terms of titre, yield, and selectivity. Regarding the effect of initial glycerol concentration in batch runs, no substrate inhibition was detected in the studied conditions (concentrations between 45 and 250 g/L were used). Employing pure glycerol as carbon source, 82.0 g/L 2,3-butanediol titre was achieved, whereas 76.5 g/L were reached using raw glycerol. These numbers involve an achieved yield respect to maximal theoretic yield of 79% and 77%, respectively. A successful kinetic modelling of the bioprocess was developed and it is able to describe both the evolution of concentrations of relevant components with time and the rates calculated at experimental time values. Estimated specific 2,3-butanediol production rate was 0.034 g₂,₃₋BDO/gX·h, while the estimated empirical pseudo-stoichiometric coefficient glycerol/2,3-butanediol was 2.52 gGₗy/g₂,₃₋BDO. The obtained results showed that R. terrigena resting cells is a promising biocatalyst, which provides new opportunities for developing and scaling-up glycerol biorefineries.
Keywords	Raoultella terrigena ; biocatalysts ; bioprocessing ; biorefining ; carbon ; evolution ; fluid mechanics ; glycerol
Language	English
Dates of publication	2021-12
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2012139-8
ISSN	1369-703X
ISSN	1369-703X
DOI	10.1016/j.bej.2021.108185
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Study on the Operational Modes Using Both Growing and Resting Cells for Succinic Acid Production from Xylose Kinetic Modelling

Escanciano, Itziar A. / Ripoll, Vanessa / Ladero, Miguel / Santos, Victoria E.

Fermentation. 2023 July 14, v. 9, no. 7

2023

Abstract: Succinic acid (SA) is one of the most prominent C4 biomass-based platform chemicals that can be biologically obtained. This article verifies, for the first time, the possibility of producing succinic acid with fed-batch or repeated batch operations with ... ...

Abstract	Succinic acid (SA) is one of the most prominent C4 biomass-based platform chemicals that can be biologically obtained. This article verifies, for the first time, the possibility of producing succinic acid with fed-batch or repeated batch operations with Actinobacillus succinogenes in a resting state, that is, in the absence of a nitrogen source. In this work it is possible to optimise separately the stages of cell growth and production in the fed-batch or repeated batch modes, minimising the costs associated with the nitrogen source and facilitating the subsequent purification of SA. These experiments were carried out with xylose, the most abundant monosaccharide in hemicelluloses, with the results subsequently being compared to those obtained in equivalent operations carried out with cells in a state of growth. First, a cost-effective synthetic growth medium was proposed and successfully employed for SA production. Biocatalysts’ reutilisation showed that the bioprocess can be carried out successfully in repeated batch and fed-batch modes. The best mode for growing cells is repeated batch, achieving a maximum productivity of 0.77 g‧L⁻¹‧h⁻¹, a selectivity of 53% and a yield of 51% with respect to xylose consumed. In contrast, the fed-batch mode was found to be the most convenient mode with resting cell biocatalyst, reaching a maximum productivity of 0.83 g‧L⁻¹‧h⁻¹, a selectivity of 0.78 g‧g⁻¹ and a yield of 68% with respect to the xylose consumed. In addition, by-product formation is significantly reduced when employing resting cells. An unstructured non-segregated kinetic model was developed for both biocatalysts, capable of simulating cell growth, xylose consumption, SA production and by-product generation, with successful estimation of kinetic parameters supported by statistical criteria.
Keywords	Actinobacillus succinogenes ; biocatalysts ; bioprocessing ; byproducts ; cell growth ; cost effectiveness ; culture media ; fermentation ; hemicellulose ; kinetics ; nitrogen ; succinic acid ; xylose
Language	English
Dates of publication	2023-0714
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2813985-9
ISSN	2311-5637
ISSN	2311-5637
DOI	10.3390/fermentation9070663
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Teaching Chemical Engineering to Biotechnology students in the time of COVID-19

Ripoll, Vanessa / Godino-Ojer, Marina / Calzada, Javier

Education for Chemical Engineers ; ISSN 1749-7728

assessment of the adaptation to digitalization

2020

Keywords	General Chemical Engineering ; Education ; covid19
Language	English
Publisher	Elsevier BV
Publishing country	us
Document type	Article ; Online
DOI	10.1016/j.ece.2020.11.001
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides.

Maranha, Ana / Costa, Mafalda / Ripoll-Rozada, Jorge / Manso, José A / Miranda, Vanessa / Mendes, Vera M / Manadas, Bruno / Macedo-Ribeiro, Sandra / Ventura, M Rita / Pereira, Pedro José Barbosa / Empadinhas, Nuno

Communications biology

2023 Volume 6, Issue 1, Page(s) 108

Abstract: The steep increase in nontuberculous mycobacteria (NTM) infections makes understanding their unique physiology an urgent health priority. NTM synthesize two polysaccharides proposed to modulate fatty acid metabolism: the ubiquitous 6-O-methylglucose ... ...

Abstract	The steep increase in nontuberculous mycobacteria (NTM) infections makes understanding their unique physiology an urgent health priority. NTM synthesize two polysaccharides proposed to modulate fatty acid metabolism: the ubiquitous 6-O-methylglucose lipopolysaccharide, and the 3-O-methylmannose polysaccharide (MMP) so far detected in rapidly growing mycobacteria. The recent identification of a unique MMP methyltransferase implicated the adjacent genes in MMP biosynthesis. We report a wide distribution of this gene cluster in NTM, including slowly growing mycobacteria such as Mycobacterium avium, which we reveal to produce MMP. Using a combination of MMP purification and chemoenzymatic syntheses of intermediates, we identified the biosynthetic mechanism of MMP, relying on two enzymes that we characterized biochemically and structurally: a previously undescribed α-endomannosidase that hydrolyses MMP into defined-sized mannoligosaccharides that prime the elongation of new daughter MMP chains by a rare α-(1→4)-mannosyltransferase. Therefore, MMP biogenesis occurs through a partially conservative replication mechanism, whose disruption affected mycobacterial growth rate at low temperature.
MeSH term(s)	Mycobacterium/genetics ; Lipopolysaccharides ; Mannosyltransferases ; Methyltransferases
Chemical Substances	methylmannoside (51023-63-3) ; Lipopolysaccharides ; Mannosyltransferases (EC 2.4.1.-) ; Methyltransferases (EC 2.1.1.-)
Language	English
Publishing date	2023-01-27
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2399-3642
ISSN (online)	2399-3642
DOI	10.1038/s42003-023-04448-3
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Teaching Chemical Engineering to Biotechnology students in the time of COVID-19: assessment of the adaptation to digitalization

Ripoll, Vanessa / Godino-Ojer, Marina / Calzada, Javier

Abstract: With the global outbreak of COVID-19 in March 2020, there was an immediate shutdown of face-to-face classes and a sudden shift to on-line learning. Confinement required finding innovative approaches to teaching and student assessment. This paper aims to ... ...

Abstract	With the global outbreak of COVID-19 in March 2020, there was an immediate shutdown of face-to-face classes and a sudden shift to on-line learning. Confinement required finding innovative approaches to teaching and student assessment. This paper aims to share the experience of adapting the course in Biochemical Engineering, part of the Biotechnology program at Francisco de Vitoria University (Madrid, Spain), to remote learning. A sequence of collaborative learning activities, with active student participation, was designed to replace the traditional mid-term exam. Activities were carefully implemented, considering the range of learning styles. Engineering skills, transversal competences and higher-order thinking skills were fostered through these activities. The analysis of the teaching/learning experience was based on teacher observations, academic performance and student surveys. All indicators showed that the adopted methodology had a positive impact of student performance. Student participation, especially among those repeating the course, also improved. Furthermore, students gained a more accurate and positive perception of the link between Chemical Engineering and Biotechnology, which may have a favourable impact on the teaching of Bioreactors in the coming academic year.
Keywords	covid19
Publisher	Elsevier
Document type	Article ; Online
DOI	10.1016/j.ece.2020.11.005
Database	COVID19

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Article ; Online: Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides

Ana Maranha / Mafalda Costa / Jorge Ripoll-Rozada / José A. Manso / Vanessa Miranda / Vera M. Mendes / Bruno Manadas / Sandra Macedo-Ribeiro / M. Rita Ventura / Pedro José Barbosa Pereira / Nuno Empadinhas

Communications Biology, Vol 6, Iss 1, Pp 1-

2023 Volume 17

Abstract: The mechanism of methylmannose polysaccharide (MMP) biogenesis in nontuberculous mycobacteria (NTM) is characterized, which involves an MMP-specific hydrolase (MmpH) and a rare mannosyltransferase (ManT). MmpH and ManT structures are presented. ...

Abstract	The mechanism of methylmannose polysaccharide (MMP) biogenesis in nontuberculous mycobacteria (NTM) is characterized, which involves an MMP-specific hydrolase (MmpH) and a rare mannosyltransferase (ManT). MmpH and ManT structures are presented.
Keywords	Biology (General) ; QH301-705.5
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: High 2,3-butanediol production from glycerol by Raoultella terrigena CECT 4519.

Ripoll, Vanessa / Rodríguez, Alberto / Ladero, Miguel / Santos, Victoria E

Bioprocess and biosystems engineering

2019 Volume 43, Issue 4, Page(s) 685–692

Abstract: Bioconversion of biodiesel-derived glycerol into 2,3-butanediol has received recently much attention due to its increasing surplus and its multiple uses in industry as bulk chemical. The influence of initial glycerol concentration on 2,3-butanediol ... ...

Abstract	Bioconversion of biodiesel-derived glycerol into 2,3-butanediol has received recently much attention due to its increasing surplus and its multiple uses in industry as bulk chemical. The influence of initial glycerol concentration on 2,3-butanediol production in batch runs has been studied. A concentration higher than 140 g/L produces an inhibitory effect on the final 2,3-butanediol concentration and its production rate. In batch mode, the highest yield respect to the theoretical maximum yield (71%) was reached employing 140 g/L as initial concentration 140 g/L. Based on these results, a high 2,3-butanediol production has been achieved through a fed-batch strategy. The reached 2,3-butanediol concentration was 90.5 g/L from pure glycerol and 80.5 g/L from raw glycerol. The 2,3-butanediol yield respect to the theoretical maximum yield was also improved through the fed-batch operation (90%). To date, this concentration is the highest produced amount employing as biocatalyst a non-pathogenic bacterium (level 1).
MeSH term(s)	Batch Cell Culture Techniques ; Bioreactors ; Butylene Glycols/metabolism ; Enterobacteriaceae/metabolism ; Glycerol/metabolism
Chemical Substances	Butylene Glycols ; 2,3-butylene glycol (45427ZB5IJ) ; Glycerol (PDC6A3C0OX)
Language	English
Publishing date	2019-12-17
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1476357-6
ISSN	1615-7605 ; 1432-0797 ; 1615-7591
ISSN (online)	1615-7605 ; 1432-0797
ISSN	1615-7591
DOI	10.1007/s00449-019-02266-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Improvement of biomethane potential of sewage sludge anaerobic co-digestion by addition of “sherry-wine” distillery wastewater

Ripoll, Vanessa / Agabo-García, Cristina / Perez, Montserrat / Solera, Rosario

Journal of cleaner production. 2020 Apr. 01, v. 251

2020

Abstract: Co-digestion of sewage sludge (SS) with other unusually treated residues has been reported as an efficient method to improve biomethane production. In this work, Sherry-wine distillery wastewater (SW-DW) has been proposed as co-substrate in order to ... ...

Abstract	Co-digestion of sewage sludge (SS) with other unusually treated residues has been reported as an efficient method to improve biomethane production. In this work, Sherry-wine distillery wastewater (SW-DW) has been proposed as co-substrate in order to increase biomethane production and as a breakthrough solution in the management of both types of waste. In order to achieve this goal, different SS:SW-DW mixtures were employed as substrates in Biomethane Potential (BMP) tests. The biodegradability and biomethane potential of each mixture was determined selecting the optimal co-substrate ratio. Results showed that the addition of SW-DW as a co-substrate improves the anaerobic digestion of SS in a proportionally way in terms of CODs and biomethane production The optimal co-substrates ratio was 50:50 of SS:SW-DW obtaining %VSremoval = 54.5%; YCH4 = 225.1 L CH4/kgsv or 154 L CH4/kgCODt and microbial population of 5.5 times higher than sole SS. In this case, %VSremoval = 48.1%; YCH4 = 183 L CH4/kgsv or 135 L CH4/kgCODt. The modified Gompertz equation was used for the kinetic modelling of biogas production with successful fitting results (r2 = 0.99). In this sense, at optimal conditions, the maximum productivity reached at an infinite digestion time was (YCH4MAX) = 229 ± 5.0 NL/kgSV; the specific constant was K = 25.0 ± 2.3 NL/kgSV·d and the lag phase time constant was (λ) = 2.49 ± 0.19.
Keywords	anaerobic digestion ; biodegradability ; biogas ; chemical oxygen demand ; distillery effluents ; equations ; gas production (biological) ; methane ; models ; sewage sludge
Language	English
Dates of publication	2020-0401
Publishing place	Elsevier Ltd
Document type	Article
ISSN	0959-6526
DOI	10.1016/j.jclepro.2019.119667
Database	NAL-Catalogue (AGRICOLA)

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Article: High 2,3-butanediol production from glycerol by Raoultella terrigena CECT 4519

Ripoll, Vanessa / Rodríguez, Alberto / Ladero, Miguel / Santos, Victoria E

Bioprocess and biosystems engineering. 2020 Apr., v. 43, no. 4

2020

Abstract	Bioconversion of biodiesel-derived glycerol into 2,3-butanediol has received recently much attention due to its increasing surplus and its multiple uses in industry as bulk chemical. The influence of initial glycerol concentration on 2,3-butanediol production in batch runs has been studied. A concentration higher than 140 g/L produces an inhibitory effect on the final 2,3-butanediol concentration and its production rate. In batch mode, the highest yield respect to the theoretical maximum yield (71%) was reached employing 140 g/L as initial concentration 140 g/L. Based on these results, a high 2,3-butanediol production has been achieved through a fed-batch strategy. The reached 2,3-butanediol concentration was 90.5 g/L from pure glycerol and 80.5 g/L from raw glycerol. The 2,3-butanediol yield respect to the theoretical maximum yield was also improved through the fed-batch operation (90%). To date, this concentration is the highest produced amount employing as biocatalyst a non-pathogenic bacterium (level 1).
Keywords	Raoultella terrigena ; bacteria ; biocatalysts ; biotransformation ; glycerol ; industry
Language	English
Dates of publication	2020-04
Size	p. 685-692.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	1476357-6
ISSN	1432-0797 ; 1615-7605 ; 1615-7591
ISSN (online)	1432-0797 ; 1615-7605
ISSN	1615-7591
DOI	10.1007/s00449-019-02266-8
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Apoplejía hipofisaria en pacientes pediátricos: revisión sistemática.

Picón Jaimes, Yelson Alejandro / Orozco Chinome, Javier Esteban / López Cepeda, Daniela / Quintana Pájaro, Loraine / Galindo-Velásquez, Hather / Ramos-Villegas, Yancarlos / Ripoll-Zapata, Vanessa / Morales-Núñez, María Angélica / Moscote Salazar, Luis Rafael

Revista de la Facultad de Ciencias Medicas (Cordoba, Argentina)

2022 Volume 79, Issue 2, Page(s) 141–145

Abstract: Introduction: Necrosis or hemorrhage associated with pituitary tumors is an infrequent complication. The term pituitary apoplexy is related to the development of an inflammatory process that leads to irritation of the meningeal and periselar areas. ... ...

Title translation	Pituitary apoplexy in pediatric patients: systematic review.
Abstract	Introduction: Necrosis or hemorrhage associated with pituitary tumors is an infrequent complication. The term pituitary apoplexy is related to the development of an inflammatory process that leads to irritation of the meningeal and periselar areas. Regardless of the mechanism (hemorrhage or infarction), the extent of the hemorrhage and necrosis will produce an increase in intraselar pressure which, in turn, will lead to a more or less pronounced compression of neighboring structures, thus explaining the broad spectrum clinical. Methods: We carried out a systematic review of cases published internationally. The analysis of 8 cases published in the literature was carried out, 5 cases in the male pediatric population (62.5%), and 3 cases of the female gender (37.5%). Results: The mean age was 12.8 years (SD ± 2.5). The clinical manifestations included: headache, impaired vision, nausea and vomiting, fever, cranial nerve involvement, and, to a lesser extent, compromise of consciousness. Seventy-five percent (n = 6) of the patients had a headache, 16.6% had a frontal location, 16.6% accompanied by retroorbital pain, and 66.6% did not specify the location. On the other hand, only 37.5% of the patients had nausea and/or vomiting. While 25% were admitted with fever and 12.5% (n = 1) had changes in the state of consciousness. Conclusion: Pituitary apoplexy is a pathology that requires a multidisciplinary approach at the pediatric level. Management directed by pediatric neurosurgery and endocrinology will allow us to offer our patients the best, evidence-based treatment available.
MeSH term(s)	Child ; Humans ; Pituitary Apoplexy ; Retrospective Studies
Language	Spanish
Publishing date	2022-06-06
Publishing country	Argentina
Document type	Journal Article ; Systematic Review
ZDB-ID	390127-0
ISSN	1853-0605 ; 0014-6722 ; 0301-7281
ISSN (online)	1853-0605
ISSN	0014-6722 ; 0301-7281
DOI	10.31053/1853.0605.v79.n2.27984
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