Article: Vasodilation of isolated vessels and the isolation of the extracellular matrix of tight-skin mice
Journal of visualized experiments. 2017 Mar. 24, , no. 121
2017
Abstract: The interferon regulatory factor 5 (IRF5) is crucial for cells to determine if they respond in a pro-inflammatory or anti-inflammatory fashion. IRF5's ability to switch cells from one pathway to another is highly attractive as a therapeutic target. We ... ...
Abstract | The interferon regulatory factor 5 (IRF5) is crucial for cells to determine if they respond in a pro-inflammatory or anti-inflammatory fashion. IRF5's ability to switch cells from one pathway to another is highly attractive as a therapeutic target. We designed a decoy peptide IRF5D with a molecular modeling software for designing small molecules and peptides. IRF5D inhibited IRF5, reduced alterations in extracellular matrix, and improved endothelial vasodilation in the tight-skin mouse (Tsk/+). The Kd of IRF5D for recombinant IRF5 is 3.72 ± 0.74 x 10-6 M as determined by binding experiments using biolayer interferometry experiments. Endothelial cells (EC) proliferation and apoptosis were unchanged using increasing concentrations of IRF5D (0 to 100 μg/mL, 24 h). Tsk/+ mice were treated with IRF5D (1 mg/kg/d subcutaneously, 21 d). IRF5 and ICAM expressions were decreased after IRF5D treatment. Endothelial function was improved as assessed by vasodilation of facialis arteries from Tsk/+ mice treated with IRF5D compared to Tsk/+ mice without IRF5D treatment. As a transcription factor, IRF5 traffics from the cytosol to the nucleus. Translocation was assessed by immunohistochemistry on cardiac myocytes cultured on the different cardiac extracellular matrices. IRF5D treatment of the Tsk/+ mouse resulted in a reduced number of IRF5 positive nuclei in comparison to the animals without IRF5D treatment (50 μg/mL, 24 h). These findings demonstrate the important role that IRF5 plays in inflammation and fibrosis in Tsk/+ mice. |
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Keywords | apoptosis ; arteries ; cardiomyocytes ; computer software ; cytosol ; endothelial cells ; extracellular matrix ; fibrosis ; immunohistochemistry ; inflammation ; interferometry ; interferon regulatory factor-5 ; mice ; molecular models ; peptides ; therapeutics ; vasodilation |
Language | English |
Dates of publication | 2017-0324 |
Size | p. e55036. |
Publishing place | Journal of Visualized Experiments |
Document type | Article |
ZDB-ID | 2259946-0 |
ISSN | 1940-087X |
ISSN | 1940-087X |
DOI | 10.3791/55036 |
Database | NAL-Catalogue (AGRICOLA) |
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