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Article: The Effect of 3D Printing Tilt Angle on the Penetration of 3D-Printed Microneedle Arrays.

Razzaghi, Mahmood / Akbari, Mohsen

2023 Volume 14, Issue 6

Abstract: Microneedle arrays (MNAs) are emerging devices that are mainly used for drug delivery and diagnostic applications through the skin. Different methods have been used to fabricate MNAs. Recently developed fabrication methods based on 3D printing have many ... ...

Abstract	Microneedle arrays (MNAs) are emerging devices that are mainly used for drug delivery and diagnostic applications through the skin. Different methods have been used to fabricate MNAs. Recently developed fabrication methods based on 3D printing have many advantages compared to conventional fabrication methods, such as faster fabrication in one step and the ability to fabricate complex structures with precise control over their geometry, form, size, and mechanical and biological properties. Despite the several advantages that 3D printing offers for the fabrication of microneedles, their poor penetration capability into the skin should be improved. MNAs need a sharp needle tip to penetrate the skin barrier layer, the stratum corneum (SC). This article presents a method to improve the penetration of 3D-printed microneedle arrays by investigating the effect of the printing angle on the penetration force of MNAs. The penetration force needed to puncture the skin for MNAs fabricated using a commercial digital light processing (DLP) printer, with different printing tilt angles (0-60°), was measured in this study. The results showed that the minimum puncture force was achieved using a 45° printing tilt angle. Using this angle, the puncture force was reduced by 38% compared to MNAs printed with a tilting angle of 0°. We also identified that a tip angle of 120° resulted in the smallest penetration force needed to puncture the skin. The outcomes of the research show that the presented method can significantly improve the penetration capability of 3D-printed MNAs into the skin.
Language	English
Publishing date	2023-05-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2620864-7
ISSN	2072-666X
ISSN	2072-666X
DOI	10.3390/mi14061157
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Immunohistochemistry (IHC) staining of in-vitro cancer cell-generated tumoroids.

Amereh, Meitham / Akbari, Mohsen

MethodsX

2023 Volume 10, Page(s) 102242

Abstract: Targeting different pathways in combinational therapy may lead to synergistic effects with higher drug efficiency. Due to a large number of candidate drugs and the variability in the genomic landscape of the disease, conventional cell culture models have ...

Abstract	Targeting different pathways in combinational therapy may lead to synergistic effects with higher drug efficiency. Due to a large number of candidate drugs and the variability in the genomic landscape of the disease, conventional cell culture models have limited success. Three-dimensional (3D) cell culture platforms such as tumoroids not only provide a pathophysiological relevant condition but also allow for low-cost and high-throughput drug screening strategies. Immunostaining of targeted proteins within a tumoroid is challenging as the interior cells are difficult to access via a non-destructive method. Immunohistochemistry (IHC) is an important technique in clinical research to explore the expression of various biomarkers. IHC staining of tumoroids allows non-destructive detection of unstable proteins by direct fixation of cells at the state of tumor microenvironment (TME) context, providing two main advantages. First, the target protein can be fixed without dissociating cells and disintegration of tumoroids into a single-cell suspension. Second, staining the preserved structure of tumoroids helps identify the location of the target proteins as well as the spatial distribution throughout the tumoroid geometry. In this protocol, we describe the detailed methodology of a non-destructive IHC staining of cancer biomarkers which minimizes the manipulation of tumoroids prior to fixation by eliminating multiple centrifugations and shaking steps typically required for removing excess hydrogel and collecting tumoroids. The protocol can be used in studies involving prognostic and predictive biomarker investigations in new anti-tumor drug development strategies.
Language	English
Publishing date	2023-06-03
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2830212-6
ISSN	2215-0161
ISSN	2215-0161
DOI	10.1016/j.mex.2023.102242
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Investigating the temperature distribution behavior and flow parameters of argon fluid in a nanochannel with changing dimensions of the obstacle using the molecular dynamics (MD) method.

Akbari, Omid Ali / Shirani, Ebrahim / Saghafian, Mohsen

Heliyon

2024 Volume 10, Issue 2, Page(s) e24065

Abstract: This article, examines the flow of argon inside a nanochannel with respect to the molecular dynamics (MD) in the free molecular flow regime using LAMMPS software. The nanochannel is made of copper featuring a square cross-section and obstacles of varying ...

Abstract	This article, examines the flow of argon inside a nanochannel with respect to the molecular dynamics (MD) in the free molecular flow regime using LAMMPS software. The nanochannel is made of copper featuring a square cross-section and obstacles of varying dimensions and values. In this study, the flow of argon fluid is three-dimensional. To gain a deeper understanding of the effect of solid walls within the nanochannel and their influence on flow behavior, the research is simulated in a nanochannel with all side walls for the 3D model and without side walls for the 2D model. This research assesses the effect of the obstacles' dimensions and values on the nanochannel wall surface and areas above the wall surface. The total dimensions of all simulated two- and three-dimensional atomic structures with a square cross-section are assumed to be 60 × 60 × 100 Å
Language	English
Publishing date	2024-01-06
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2024.e24065
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Article ; Online: Immunohistochemistry (IHC) staining of in-vitro cancer cell-generated tumoroids

Meitham Amereh / Mohsen Akbari

MethodsX, Vol 10, Iss , Pp 102242- (2023)

2023

Abstract	Targeting different pathways in combinational therapy may lead to synergistic effects with higher drug efficiency. Due to a large number of candidate drugs and the variability in the genomic landscape of the disease, conventional cell culture models have limited success. Three-dimensional (3D) cell culture platforms such as tumoroids not only provide a pathophysiological relevant condition but also allow for low-cost and high-throughput drug screening strategies. Immunostaining of targeted proteins within a tumoroid is challenging as the interior cells are difficult to access via a non-destructive method. Immunohistochemistry (IHC) is an important technique in clinical research to explore the expression of various biomarkers. IHC staining of tumoroids allows non-destructive detection of unstable proteins by direct fixation of cells at the state of tumor microenvironment (TME) context, providing two main advantages. First, the target protein can be fixed without dissociating cells and disintegration of tumoroids into a single-cell suspension. Second, staining the preserved structure of tumoroids helps identify the location of the target proteins as well as the spatial distribution throughout the tumoroid geometry. In this protocol, we describe the detailed methodology of a non-destructive IHC staining of cancer biomarkers which minimizes the manipulation of tumoroids prior to fixation by eliminating multiple centrifugations and shaking steps typically required for removing excess hydrogel and collecting tumoroids. The protocol can be used in studies involving prognostic and predictive biomarker investigations in new anti-tumor drug development strategies.
Keywords	Immunohistochemistry (IHC) staining of in-vitro cancer cell-generated tumoroids ; Science ; Q
Subject code	500
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Tissue bioprinting for biology and medicine.

Akbari, Mohsen / Khademhosseini, Ali

Cell

2022 Volume 185, Issue 15, Page(s) 2644–2648

Abstract: Bioprinting merges additive manufacturing and tissue engineering to generate functional tissues and organs. The field has experienced tremendous growth over the past few years. Here, we highlight recent breakthroughs in bioprinting and discuss the ... ...

Abstract	Bioprinting merges additive manufacturing and tissue engineering to generate functional tissues and organs. The field has experienced tremendous growth over the past few years. Here, we highlight recent breakthroughs in bioprinting and discuss the challenges that are yet to be addressed before this technology can be widely utilized in biology and medicine.
MeSH term(s)	Biology ; Bioprinting ; Printing, Three-Dimensional ; Regenerative Medicine ; Tissue Engineering
Language	English
Publishing date	2022-07-19
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	187009-9
ISSN	1097-4172 ; 0092-8674
ISSN (online)	1097-4172
ISSN	0092-8674
DOI	10.1016/j.cell.2022.06.015
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Article ; Online: Investigating the temperature distribution behavior and flow parameters of argon fluid in a nanochannel with changing dimensions of the obstacle using the molecular dynamics (MD) method

Omid Ali Akbari / Ebrahim Shirani / Mohsen Saghafian

Heliyon, Vol 10, Iss 2, Pp e24065- (2024)

2024

Abstract	This article, examines the flow of argon inside a nanochannel with respect to the molecular dynamics (MD) in the free molecular flow regime using LAMMPS software. The nanochannel is made of copper featuring a square cross-section and obstacles of varying dimensions and values. In this study, the flow of argon fluid is three-dimensional. To gain a deeper understanding of the effect of solid walls within the nanochannel and their influence on flow behavior, the research is simulated in a nanochannel with all side walls for the 3D model and without side walls for the 2D model. This research assesses the effect of the obstacles’ dimensions and values on the nanochannel wall surface and areas above the wall surface. The total dimensions of all simulated two- and three-dimensional atomic structures with a square cross-section are assumed to be 60 × 60 × 100 Å3. and the presence of square obstacles (with dimensions of 8 × 8 × 8 Å3) and rectangular obstacles (with dimensions of 8 × 18 × 8 Å3) is examined. This study seeks to understand the influence on flow behavior, temperature distribution, density, heat flux, velocity, and thermal conductivity coefficient. This study is simulated using a time step of 1 fs for 10,000 time steps, involving approximately 10,000–15,000 argon and copper atoms. The results of this research indicate that obstacles with structures of P and R and larger dimensions increase the number of solid atoms exhibiting stronger attractive forces. Compared to a smooth nanochannel, the thermal exchange between fluid and solid atoms results in a density increase of 17.5 % and 17.3 %, respectively. On the other hand, in the 3D nanochannel, the sidewalls of the nanochannel have reduced the effect of the presence of R and P obstacles with larger dimensions, which comparing to a smooth nanochannel, have increased the density by 8.21 % and 7.53 %, respectively. The obstacles with different spatial positions (P and R structures) in the two-dimensional nanochannel cause a rise in the thermal conductivity ...
Keywords	Nanochannel ; LAMMPS software ; Rectangular obstacles ; Density distribution ; Heat flux ; Molecular dynamics ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
Subject code	541
Language	English
Publishing date	2024-01-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: In Vitro Glioblastoma Model on a Plate for Localized Drug Release Study from a 3D-Printed Drug-Eluted Hydrogel Mesh.

Chehri, Behnad / Liu, Kaiwen / Vaseghi, Golnaz / Seyfoori, Amir / Akbari, Mohsen

Cells

2024 Volume 13, Issue 4

Abstract: Glioblastoma multiforme (GBM) is an aggressive type of brain tumor that has limited treatment options. Current standard therapies, including surgery followed by radiotherapy and chemotherapy, are not very effective due to the rapid progression and ... ...

Abstract	Glioblastoma multiforme (GBM) is an aggressive type of brain tumor that has limited treatment options. Current standard therapies, including surgery followed by radiotherapy and chemotherapy, are not very effective due to the rapid progression and recurrence of the tumor. Therefore, there is an urgent need for more effective treatments, such as combination therapy and localized drug delivery systems that can reduce systemic side effects. Recently, a handheld printer was developed that can deliver drugs directly to the tumor site. In this study, the feasibility of using this technology for localized co-delivery of temozolomide (TMZ) and deferiprone (DFP) to treat glioblastoma is showcased. A flexible drug-loaded mesh (GlioMesh) loaded with poly (lactic-co-glycolic acid) (PLGA) microparticles is printed, which shows the sustained release of both drugs for up to a month. The effectiveness of the printed drug-eluting mesh in terms of tumor toxicity and invasion inhibition is evaluated using a 3D micro-physiological system on a plate and the formation of GBM tumoroids within the microenvironment. The proposed in vitro model can identify the effective combination doses of TMZ and DFP in a sustained drug delivery platform. Additionally, our approach shows promise in GB therapy by enabling localized delivery of multiple drugs, preventing off-target cytotoxic effects.
MeSH term(s)	Humans ; Glioblastoma/drug therapy ; Glioblastoma/pathology ; Hydrogels/therapeutic use ; Drug Liberation ; Temozolomide/therapeutic use ; Printing, Three-Dimensional ; Tumor Microenvironment
Chemical Substances	Hydrogels ; Temozolomide (YF1K15M17Y)
Language	English
Publishing date	2024-02-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2661518-6
ISSN	2073-4409 ; 2073-4409
ISSN (online)	2073-4409
ISSN	2073-4409
DOI	10.3390/cells13040363
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Article: Tissue bioprinting for biology and medicine

Akbari, Mohsen / Khademhosseini, Ali

Cell. 2022 July 21, v. 185, no. 15

2022

Abstract	Bioprinting merges additive manufacturing and tissue engineering to generate functional tissues and organs. The field has experienced tremendous growth over the past few years. Here, we highlight recent breakthroughs in bioprinting and discuss the challenges that are yet to be addressed before this technology can be widely utilized in biology and medicine.
Keywords	additives ; bioprinting ; cells ; fields ; manufacturing ; medicine ; tissue engineering ; tissues
Language	English
Dates of publication	2022-0721
Size	p. 2644-2648.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	187009-9
ISSN	1097-4172 ; 0092-8674
ISSN (online)	1097-4172
ISSN	0092-8674
DOI	10.1016/j.cell.2022.06.015
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: In-silico study of asymmetric remodeling of tumors in response to external biochemical stimuli.

Amereh, Meitham / Akbari, Mohsen / Nadler, Ben

Scientific reports

2023 Volume 13, Issue 1, Page(s) 941

Abstract: Among different hallmarks of cancer, understanding biomechanics of tumor growth and remodeling benefits the most from the theoretical framework of continuum mechanics. Tumor remodeling initiates when cancer cells seek new homeostasis in response to the ... ...

Abstract	Among different hallmarks of cancer, understanding biomechanics of tumor growth and remodeling benefits the most from the theoretical framework of continuum mechanics. Tumor remodeling initiates when cancer cells seek new homeostasis in response to the microenvironmental stimuli. Cells within a growing tumor are capable to remodel their inter- and intra-connections and become more mobile to achieve a new homeostasis. This mobility enables the tumor to undergo large deformation. In this work, we studied the remodeling of homogeneous tumors, at their early stage of growth, in the context of continuum mechanics. We developed an evolution law for the remodeling-associated deformation which correlates the remodeling to a characteristic tensor of external stimuli. The asymmetric remodeling and the induced mechanical stresses were analyzed for different types of biochemical distributions. To experimentally investigate the model, we studied the remodeling of human glioblastoma (hGB) tumoroids in response to the gradient of nutrients. Using a tumoroid-on-a-chip platform, the degree of remodeling was estimated for the ellipsoidal tumoroids over time. It was observed that higher gradient of nutrients induces higher degree of ellipticity suggesting that the gradient of nutrient is a characteristic property of nutrient distribution that derives the remodeling. We also showed that remodeling gives rise to heterogeneity in cell distribution forming circumferentially aligned cells within the tumors. Compared to the existing studies on tumor growth, our work provides a biomechanical module that relates the remodeling to biochemical stimuli, and allows for large deformation. It also includes experimental component, a necessary but challenging step, that connects the theory and reality to evaluate the practicability of the model.
MeSH term(s)	Humans ; Biomechanical Phenomena ; Stress, Mechanical ; Glioblastoma
Language	English
Publishing date	2023-01-18
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-26891-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Insight into the roles of hematite iron oxide nanoparticles on microalgae growth, urban wastewater treatment and bioproducts generation: Gompertz simulation, nutrient mass balance and gene expression.

Akbari, Sara / Zabihollahi, Shaghayegh / Yaqoubnejad, Poone / Palandi, Zahra Khodabakhshi / Taghavijeloudar, Mohsen

Bioresource technology

2024 Volume 394, Page(s) 130300

Abstract: In this study, the effect of α- ... ...

Abstract	In this study, the effect of α-Fe
MeSH term(s)	Microalgae/metabolism ; Nutrients ; Biomass ; Water Purification ; Magnetic Iron Oxide Nanoparticles ; Gene Expression ; Chlorella vulgaris/metabolism ; Ferric Compounds
Chemical Substances	ferric oxide (1K09F3G675) ; Ferric Compounds
Language	English
Publishing date	2024-01-06
Publishing country	England
Document type	Journal Article
ZDB-ID	1065195-0
ISSN	1873-2976 ; 0960-8524
ISSN (online)	1873-2976
ISSN	0960-8524
DOI	10.1016/j.biortech.2024.130300
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Z 3258: Show issues

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