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  1. Article ; Online: Immunofluorescent Antibody Techniques in the Diagnosis of SARS-CoV-2 Infection in Humans.

    Hueston, Linda

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2452, Page(s) 99–107

    Abstract: Immunofluorescence (IF) is an important technique used in the diagnosis of many infectious diseases. In virology, it has proven to be particularly suited to detecting antibody directed against newly emerging viruses able to be cultivated in cell culture. ...

    Abstract Immunofluorescence (IF) is an important technique used in the diagnosis of many infectious diseases. In virology, it has proven to be particularly suited to detecting antibody directed against newly emerging viruses able to be cultivated in cell culture. It permits visualization of antibody and allows for antibody class to be determined which is critical to understanding the timing of infection. The procedure used to determine IgG, IgA, and IgM antibody directed against SARS-CoV-2 in humans is described in this chapter. These methods were developed for routine diagnosis of SARS-CoV-2 infection in Australia at the start of the global pandemic in 2020.
    MeSH term(s) Antibodies, Viral ; COVID-19/diagnosis ; Fluorescent Antibody Technique ; Humans ; Immunoglobulin G ; Immunoglobulin M ; SARS-CoV-2 ; Sensitivity and Specificity
    Chemical Substances Antibodies, Viral ; Immunoglobulin G ; Immunoglobulin M
    Language English
    Publishing date 2022-05-12
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2111-0_7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Seroprevalence of Japanese encephalitis virus-specific antibodies in Australia following novel epidemic spread: protocol for a national cross-sectional study.

    Winkler, Noni Ella / Koirala, Archana / Kaur, Guddu / Prasad, Shayal / Hirani, Rena / Baker, Jannah / Hoad, Veronica / Gosbell, Iain B / Irving, David O / Hueston, Linda / O'Sullivan, Matthew Vn / Kok, Jen / Dwyer, Dominic E / Macartney, Kristine

    BMJ open

    2024  Volume 14, Issue 2, Page(s) e075569

    Abstract: Introduction: Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that causes encephalitis and other morbidity in Southeast Asia. Since February 2022, geographically dispersed JEV human, animal and vector detections occurred on the ... ...

    Abstract Introduction: Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that causes encephalitis and other morbidity in Southeast Asia. Since February 2022, geographically dispersed JEV human, animal and vector detections occurred on the Australian mainland for the first time. This study will determine the prevalence of JEV-specific antibodies in human blood with a focus on populations at high risk of JEV exposure and determine risk factors associated with JEV seropositivity by location, age, occupation and other factors.
    Method: Samples are collected using two approaches: from routine blood donors (4153 samples), and active collections targeting high-risk populations (convenience sampling). Consent-based sampling for the latter includes a participant questionnaire on demographic, vaccination and exposure data. Samples are tested for JEV-specific total antibody using a defined epitope-blocking ELISA, and total antibody to Australian endemic flaviviruses Murray Valley encephalitis and Kunjin viruses.
    Analysis: Two analytic approaches will occur: descriptive estimates of seroprevalence and multivariable logistic regression using Bayesian hierarchical models. Descriptive analyses will include unadjusted analysis of raw data with exclusions for JEV-endemic country of birth, travel to JEV-endemic countries, prior JEV-vaccination, and sex-standardised and age-standardised analyses. Multivariable logistic regression will determine which risk factors are associated with JEV seropositivity likely due to recent transmission within Australia and the relative contribution of each factor when accounting for effects within the model.
    Ethics: National Mutual Acceptance ethical approval was obtained from the Sydney Children's Hospitals Network Human Research Ethics Committee (HREC). Local approvals were sought in each jurisdiction. Ethical approval was also obtained from the Australian Red Cross Lifeblood HREC.
    Dissemination: Findings will be communicated to participants and their communities, and human and animal health stakeholders and policy-makers iteratively and after final analyses. Understanding human infection rates will inform procurement and targeted allocation of limited JEV vaccine, and public health strategies and communication campaigns, to at-risk populations.
    MeSH term(s) Humans ; Animals ; Child ; Encephalitis Virus, Japanese ; Encephalitis, Japanese/epidemiology ; Encephalitis, Japanese/prevention & control ; Cross-Sectional Studies ; Seroepidemiologic Studies ; Bayes Theorem ; Australia/epidemiology ; Antibodies, Viral
    Chemical Substances Antibodies, Viral
    Language English
    Publishing date 2024-02-07
    Publishing country England
    Document type Journal Article
    ZDB-ID 2599832-8
    ISSN 2044-6055 ; 2044-6055
    ISSN (online) 2044-6055
    ISSN 2044-6055
    DOI 10.1136/bmjopen-2023-075569
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  3. Article ; Online: Inhibition of Interleukin 1β Signaling by Anakinra Ameliorates Proinflammatory Cytokine Responses in Zika Virus-Infected Human Blood-Brain Barrier Endothelial Cells.

    Hueston, Linda / Grau, Georges E / Mahalingam, Suresh

    The Journal of infectious diseases

    2019  Volume 220, Issue 9, Page(s) 1539–1540

    MeSH term(s) Blood-Brain Barrier ; Cytokines ; Endothelial Cells ; Humans ; Inflammasomes ; Inflammation ; Interleukin 1 Receptor Antagonist Protein ; Interleukin-1beta ; NLR Family, Pyrin Domain-Containing 3 Protein ; Zika Virus ; Zika Virus Infection
    Chemical Substances Cytokines ; Inflammasomes ; Interleukin 1 Receptor Antagonist Protein ; Interleukin-1beta ; NLR Family, Pyrin Domain-Containing 3 Protein ; NLRP3 protein, human
    Language English
    Publishing date 2019-05-22
    Publishing country United States
    Document type Letter ; Research Support, Non-U.S. Gov't ; Comment
    ZDB-ID 3019-3
    ISSN 1537-6613 ; 0022-1899
    ISSN (online) 1537-6613
    ISSN 0022-1899
    DOI 10.1093/infdis/jiz276
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  4. Article ; Online: Characterizing the acute antibody response of monkeypox and MVA-BN vaccine following an Australian outbreak.

    Asquith, Will / Hueston, Linda / Dwyer, Dominic / Kok, Jen / Ko, Danny / Fennel, Michael / Rockett, Rebecca / Rai, Neela Joshi / Li, Ying / Sriramoju, Shirisha / Sutor, Allison / O'Sullivan, Matthew

    Journal of medical virology

    2024  Volume 96, Issue 1, Page(s) e29407

    Abstract: In response to the emergence of the monkeypox virus (MPXV) in Australia in May 2022, we developed and evaluated indirect immunofluorescence assays (IFA) for MPXV and Vaccinia virus (VACV) IgG and IgM antibodies using serum samples from patients with ... ...

    Abstract In response to the emergence of the monkeypox virus (MPXV) in Australia in May 2022, we developed and evaluated indirect immunofluorescence assays (IFA) for MPXV and Vaccinia virus (VACV) IgG and IgM antibodies using serum samples from patients with nucleic acid amplification test (NAAT)-confirmed mpox and uninfected unvaccinated controls. Additionally, 47 healthcare workers receiving two doses of the third-generation smallpox vaccine Modified Vaccinia Ankara-Bavarian Nordic (MVA-BN) undertook serial serum collection to describe the serological response to vaccination. MPXV antibodies were detected in 16/18 individuals with NAAT-confirmed mpox (sensitivity 0.89, specificity 1.00), and VACV antibodies were detected in 28/29 individuals who received two doses of MVA-BN vaccine (sensitivity 0.97, specificity 1.00). Detectable antibody in subjects historically vaccinated with early-generation vaccines against smallpox was found in 7/7 subjects, at a median of 48 years following vaccination. MPXV NAAT-positive patients with serum samples collected within the first 14 days after rash onset had detectable IgG and IgM in 9/12 and 5/12 of patients, respectively, with maintenance of IgG and disappearance of IgM titers after 60 days. While specificity was high when testing unvaccinated and uninfected subjects, significant cross-reactivity between MPXV and VACV antibodies was observed.
    MeSH term(s) Humans ; Vaccinia virus ; Vaccinia ; Mpox (monkeypox)/epidemiology ; Mpox (monkeypox)/prevention & control ; Antibody Formation ; Australia/epidemiology ; Antibodies, Viral ; Monkeypox virus ; Immunoglobulin M ; Immunoglobulin G ; Smallpox Vaccine ; Vaccines, Attenuated
    Chemical Substances smallpox and monkeypox vaccine modified vaccinia ankara-bavarian nordic (TU8J357395) ; Antibodies, Viral ; Immunoglobulin M ; Immunoglobulin G ; Smallpox Vaccine ; Vaccines, Attenuated
    Language English
    Publishing date 2024-01-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 752392-0
    ISSN 1096-9071 ; 0146-6615
    ISSN (online) 1096-9071
    ISSN 0146-6615
    DOI 10.1002/jmv.29407
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  5. Article ; Online: Extending Graduation From Medical School.

    Hueston, William J / Grosse, Kerry J / Paschal, Linda L

    Academic medicine : journal of the Association of American Medical Colleges

    2018  Volume 93, Issue 3, Page(s) 345–346

    MeSH term(s) Schools, Medical ; Students, Medical
    Language English
    Publishing date 2018-02-27
    Publishing country United States
    Document type Letter ; Comment
    ZDB-ID 96192-9
    ISSN 1938-808X ; 1040-2446
    ISSN (online) 1938-808X
    ISSN 1040-2446
    DOI 10.1097/ACM.0000000000002063
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  6. Article ; Online: SARS-CoV-2-specific IgM screening has low sensitivity for identifying potentially infectious travellers.

    Hasan, Tasnim / Lim, H Ling / Hueston, Linda / Dwyer, Dominic E / O'Sullivan, Matthew

    Pathology

    2021  Volume 53, Issue 7, Page(s) 917–919

    MeSH term(s) Antibodies, Viral/analysis ; Antibodies, Viral/blood ; COVID-19/diagnosis ; COVID-19/immunology ; COVID-19/prevention & control ; COVID-19/transmission ; COVID-19 Serological Testing/methods ; COVID-19 Serological Testing/standards ; COVID-19 Serological Testing/statistics & numerical data ; Cross-Sectional Studies ; Disease Transmission, Infectious ; Humans ; Immunoglobulin M/analysis ; Immunoglobulin M/blood ; Mass Screening ; Predictive Value of Tests ; Retrospective Studies ; SARS-CoV-2/immunology ; SARS-CoV-2/isolation & purification ; Sensitivity and Specificity ; Travel
    Chemical Substances Antibodies, Viral ; Immunoglobulin M
    Language English
    Publishing date 2021-09-23
    Publishing country England
    Document type Letter
    ZDB-ID 7085-3
    ISSN 1465-3931 ; 0031-3025
    ISSN (online) 1465-3931
    ISSN 0031-3025
    DOI 10.1016/j.pathol.2021.08.002
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  7. Article ; Online: New Detection of Locally Acquired Japanese Encephalitis Virus Using Clinical Metagenomics, New South Wales, Australia.

    Maamary, Joel / Maddocks, Susan / Barnett, Yael / Wong, Stephen / Rodriguez, Michael / Hueston, Linda / Jeoffreys, Neisha / Eden, John-Sebastian / Dwyer, Dominic E / Floyd, Tony / Plit, Marshall / Kok, Jen / Brew, Bruce

    Emerging infectious diseases

    2023  Volume 29, Issue 3, Page(s) 627–630

    Abstract: In the context of an emerging Japanese encephalitis outbreak within Australia, we describe a novel locally acquired case in New South Wales. A man in his 70s had rapidly progressive, fatal meningoencephalitis, diagnosed as caused by Japanese encephalitis ...

    Abstract In the context of an emerging Japanese encephalitis outbreak within Australia, we describe a novel locally acquired case in New South Wales. A man in his 70s had rapidly progressive, fatal meningoencephalitis, diagnosed as caused by Japanese encephalitis virus by RNA-based metagenomic next-generation sequencing performed on postmortem brain tissue.
    MeSH term(s) Male ; Humans ; Encephalitis Virus, Japanese ; New South Wales ; Metagenomics ; Brain ; Australia/epidemiology ; Encephalitis, Japanese
    Language English
    Publishing date 2023-02-23
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1380686-5
    ISSN 1080-6059 ; 1080-6040
    ISSN (online) 1080-6059
    ISSN 1080-6040
    DOI 10.3201/eid2903.220632
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  8. Article ; Online: Enhancement of Zika Infection by Dengue Virus-Specific Antibody Is Associated With Low Levels of Antiviral Factors.

    Hueston, Linda / Ramirez, Ruben / Mahalingam, Suresh

    The Journal of infectious diseases

    2017  Volume 216, Issue 5, Page(s) 612–614

    MeSH term(s) Antiviral Agents ; Brazil ; Dengue Virus ; Humans ; Zika Virus ; Zika Virus Infection
    Chemical Substances Antiviral Agents
    Language English
    Publishing date 2017-09-13
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Comment
    ZDB-ID 3019-3
    ISSN 1537-6613 ; 0022-1899
    ISSN (online) 1537-6613
    ISSN 0022-1899
    DOI 10.1093/infdis/jix344
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  9. Article ; Online: SARS-CoV-2 Seroprevalence in a Cohort of International Travellers Returning to Rural Australia: Enablers and Barriers to Containment of COVID-19.

    Jackson, Justin / Chan, Chun / McBurnie, Jacqueline / La Hera-Fuentes, Gina / Burston, John / Bridges, Leisa / Underhill, Craig / Eek, Richard / Hueston, Linda / O'Sullivan, Matthew / Dwyer, Dominic E

    Australian and New Zealand journal of public health

    2023  Volume 47, Issue 1, Page(s) 100003

    Abstract: Objective: To describe the effectiveness of the public health response to COVID-19 in our local region by documenting detection of SARS-CoV-2 infection by nucleic acid testing (NAT) positivity and seroprevalence.: Methods: In this prospective study ( ... ...

    Abstract Objective: To describe the effectiveness of the public health response to COVID-19 in our local region by documenting detection of SARS-CoV-2 infection by nucleic acid testing (NAT) positivity and seroprevalence.
    Methods: In this prospective study (ACTRN12620000487910), symptomatic adult international travellers returning to regional Australia in March 2020 underwent SARS-CoV-2 NAT and SARS-CoV-2-specific serology.
    Results: Ninety-nine eligible participants were included. Nine participants had laboratory confirmed SARS-CoV-2, all returning between 16-20 March 2020. Eight (89%) had a positive NAT and seven (78%) had a positive serology test. The majority returned from New Zealand. Participants most frequently presented with cough (100%), headache (66.7%) and sore throat (44.4%). No community cases were detected from 1 March to 30 June 2020.
    Conclusions: The study cohort of international travellers returning to regional Australia in March 2020 returned eight positive SARS-CoV-2 NAT results over a five-day window. Serology identified one additional case and was negative in two cases who were PCR positive. Longitudinal data confirmed an absence of local community transmission to 30 June 2020.
    Implications for public health: A combination of local, national and environmental factors were necessary to prevent the establishment of community transmission in our local region.
    MeSH term(s) Adult ; Humans ; COVID-19/diagnosis ; COVID-19/epidemiology ; SARS-CoV-2 ; Seroepidemiologic Studies ; Prospective Studies ; Rural Population
    Language English
    Publishing date 2023-01-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1323548-5
    ISSN 1753-6405 ; 1326-0200
    ISSN (online) 1753-6405
    ISSN 1326-0200
    DOI 10.1016/j.anzjph.2022.100003
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  10. Article: The increase in presentations of dengue fever in New South Wales.

    Hueston, Linda

    New South Wales public health bulletin

    2005  Volume 15, Issue 11-12, Page(s) 204–207

    MeSH term(s) Aedes/virology ; Animals ; Dengue/epidemiology ; Disease Notification ; Humans ; Incidence ; Insect Vectors/virology ; New South Wales/epidemiology ; Prevalence ; Surveys and Questionnaires
    Language English
    Publishing date 2005-02-11
    Publishing country Australia
    Document type Journal Article
    ZDB-ID 2114630-5
    ISSN 1034-7674
    ISSN 1034-7674
    DOI 10.1071/nb04044
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